Abhishek Srivastava, Jayaseelan Murugaiyan, Juan A. L. Garcia, Daniele De Corte, Matthias Hoetzinger, Murat Eravci, Christoph Weise, Yadhu Kumar, Uwe Roesler, Martin W. Hahn, Hans-Peter Grossart
- Polynucleobacter asymbioticus strain QLW-P1DMWA-1T represents a group of highly successful heterotrophic ultramicrobacteria that is frequently very abundant (up to 70% of total bacterioplankton) in freshwater habitats across all seven continents. This strain was originally isolated from a shallow Alpine pond characterized by rapid changes in water temperature and elevated UV radiation due to its location at an altitude of 1300 m. To elucidate the strain’s adjustment to fluctuating environmental conditions, we recorded changes occurring in its transcriptomic and proteomic profiles under contrasting experimental conditions by simulating thermal conditions in winter and summer as well as high UV irradiation. To analyze the potential connection between gene expression and regulation via methyl group modification of the genome, we also analyzed its methylome. The methylation pattern differed between the three treatments, pointing to its potential role in differential gene expression. An adaptive process due to evolutionary pressure in thePolynucleobacter asymbioticus strain QLW-P1DMWA-1T represents a group of highly successful heterotrophic ultramicrobacteria that is frequently very abundant (up to 70% of total bacterioplankton) in freshwater habitats across all seven continents. This strain was originally isolated from a shallow Alpine pond characterized by rapid changes in water temperature and elevated UV radiation due to its location at an altitude of 1300 m. To elucidate the strain’s adjustment to fluctuating environmental conditions, we recorded changes occurring in its transcriptomic and proteomic profiles under contrasting experimental conditions by simulating thermal conditions in winter and summer as well as high UV irradiation. To analyze the potential connection between gene expression and regulation via methyl group modification of the genome, we also analyzed its methylome. The methylation pattern differed between the three treatments, pointing to its potential role in differential gene expression. An adaptive process due to evolutionary pressure in the genus was deduced by calculating the ratios of non-synonymous to synonymous substitution rates for 20 Polynucleobacter spp. genomes obtained from geographically diverse isolates. The results indicate purifying selection.…
MetadatenAuthor details: | Abhishek Srivastava, Jayaseelan MurugaiyanORCiDGND, Juan A. L. Garcia, Daniele De CorteORCiD, Matthias HoetzingerORCiD, Murat EravciORCiD, Christoph WeiseORCiD, Yadhu Kumar, Uwe RoeslerORCiD, Martin W. HahnORCiD, Hans-Peter GrossartORCiDGND |
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URN: | urn:nbn:de:kobv:517-opus4-481993 |
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DOI: | https://doi.org/10.25932/publishup-48199 |
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ISSN: | 1866-8372 |
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Title of parent work (German): | Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe |
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Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (1011) |
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Publication type: | Postprint |
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Language: | English |
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Date of first publication: | 2020/11/11 |
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Publication year: | 2020 |
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Publishing institution: | Universität Potsdam |
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Release date: | 2020/11/11 |
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Tag: | DNA modification; UV radiation; freshwater heterotrophic bacteria; gene expression; purifying selection |
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Issue: | 1011 |
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Number of pages: | 23 |
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Source: | Frontiers in Microbiology 11 (2020) 544785 DOI: 10.3389/fmicb.2020.544785 |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Peer review: | Referiert |
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Publishing method: | Open Access / Green Open-Access |
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License (German): | CC-BY - Namensnennung 4.0 International |
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External remark: | Bibliographieeintrag der Originalveröffentlichung/Quelle |
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