Gene cluster activation in a bacterial symbiont leads to halogenated angucyclic maduralactomycins and spirocyclic actinospirols
- Growth from spores activated a biosynthetic gene cluster in Actinomadura sp. RB29, resulting in the identification of two novel groups of halogenated polyketide natural products, named maduralactomycins and actinospirols. The unique tetracyclic and spirocyclic structures were assigned based on a combination of NMR analysis, chemoinformatic calculations, X-ray crystallography, and C-13 labeling studies. On the basis of HRMS2 data, genome mining, and gene expression studies, we propose an underlying noncanonical angucycline biosynthesis and extensive post-polyketide synthase (PKS) oxidative modifications.
| Author details: | Huijuan GuoGND, Jan W. SchwitallaORCiD, René BenndorfORCiDGND, Martin BaunachORCiDGND, Christoph SteinbeckORCiDGND, Helmar GörlsGND, Z. Wilhelm de BeerORCiD, Lars RegesteinORCiDGND, Christine BeemelmannsORCiDGND |
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| DOI: | https://doi.org/10.1021/acs.orglett.0c00601 |
| ISSN: | 1523-7060 |
| ISSN: | 1523-7052 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/32193935 |
| Title of parent work (English): | Organic letters |
| Publisher: | American Chemical Society |
| Place of publishing: | Washington, DC |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2020/03/20 |
| Publication year: | 2020 |
| Release date: | 2023/11/13 |
| Volume: | 22 |
| Issue: | 7 |
| Number of pages: | 5 |
| First page: | 2634 |
| Last Page: | 2638 |
| Funding institution: | German Research Foundation (DFG)German Research Foundation (DFG) [BE; 4799/2-1, BE 4799/3-1, CRC1127] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Peer review: | Referiert |
