- Treffer 1 von 1
Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration
- Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R'(SHG)) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R'(SHG) values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules. (C) 2015 Optical Society of America
| Verfasserangaben: | Richard Cisek, Danielle Tokarz, Martin SteupORCiDGND, Ian J. Tetlow, Michael J. Emes, Kim H. Hebelstrup, Andreas Blennow, Virginijus Barzda |
|---|---|
| DOI: | https://doi.org/10.1364/BOE.6.003694 |
| ISSN: | 2156-7085 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/26504621 |
| Titel des übergeordneten Werks (Englisch): | Biomedical optics express |
| Verlag: | Optical Society of America |
| Verlagsort: | Washington |
| Publikationstyp: | Wissenschaftlicher Artikel |
| Sprache: | Englisch |
| Jahr der Erstveröffentlichung: | 2015 |
| Erscheinungsjahr: | 2015 |
| Datum der Freischaltung: | 27.03.2017 |
| Band: | 6 |
| Ausgabe: | 10 |
| Seitenanzahl: | 7 |
| Erste Seite: | 3694 |
| Letzte Seite: | 3700 |
| Fördernde Institution: | Natural Sciences and Engineering Research Council of Canada (NSERC) |
| Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer Review: | Referiert |
| Publikationsweg: | Open Access |
