Low-energy (5-20 eV) electron-induced single and double strand breaks in well-defined DNA sequences
- Ionizing radiation is used in cancer radiation therapy to effectively damage the DNA of tumors. The main damage is due to generation of highly reactive secondary species such as low-energy electrons (LEEs). The accurate quantification of DNA radiation damage of well-defined DNA target sequences in terms of absolute cross sections for LEE-induced DNA strand breaks is possible by the DNA origami technique; however, to date, it is possible only for DNA single strands. In the present work DNA double strand breaks in the DNA sequence 5'-d(CAC)(4)/5'd(GTG)(4) are compared with DNA single strand breaks in the oligonucleotides 5'-d(CAC)(4) and 5'-d(GTG)(4) upon irradiation with LEEs in the energy range from 5 to 20 eV. A maximum of strand break cross section was found around 7 and 10 eV independent of the DNA sequence, indicating that dissociative electron attachment is the underlying mechanism of strand breakage and confirming previous studies using plasmid DNA.
Verfasserangaben: | Kenny EbelORCiDGND, Ilko BaldORCiDGND |
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DOI: | https://doi.org/10.1021/acs.jpclett.2c00684 |
ISSN: | 1948-7185 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/35617198 |
Titel des übergeordneten Werks (Englisch): | The journal of physical chemistry letters / American Chemical Society |
Verlag: | American Chemical Society |
Verlagsort: | Washington |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Erstveröffentlichung: | 26.05.2022 |
Erscheinungsjahr: | 2022 |
Datum der Freischaltung: | 06.02.2023 |
Band: | 13 |
Ausgabe: | 22 |
Seitenanzahl: | 6 |
Erste Seite: | 4871 |
Letzte Seite: | 4876 |
Fördernde Institution: | European Research Council (ERC) [772752]; German Research Foundation; (DFG) [230710387] |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 53 Physik / 530 Physik |
Peer Review: | Referiert |
Publikationsweg: | Open Access / Hybrid Open-Access |
Lizenz (Deutsch): | CC-BY-NC-ND - Namensnennung, nicht kommerziell, keine Bearbeitungen 4.0 International |