Extern
Refine
Year of publication
Document Type
- Article (193) (remove)
Is part of the Bibliography
- yes (193) (remove)
Keywords
- football (5)
- Reflexion (4)
- exercise (4)
- Lehrkräftebildung (3)
- kinetics (3)
- obesity (3)
- stunting (3)
- COVID-19 (2)
- Feedback (2)
- Physical activity (2)
- SEPE (2)
- SNARC (2)
- Summer Schools (2)
- achilles tendinopathy (2)
- attention (2)
- body height (2)
- climate change (2)
- diabetes (2)
- diffusion (2)
- electromyography (2)
- handgrip strength (2)
- health care (2)
- language acquisition (2)
- literature review (2)
- longitudinal (2)
- machine learning (2)
- malnutrition (2)
- maturation (2)
- pre-school (2)
- reflexion (2)
- school mathematics (2)
- skills (2)
- virtual reality (2)
- wheat (2)
- 3D thermal model (1)
- 5-methoxycarbonylmethyl-2-thiouridine (1)
- ACWR (1)
- AFLP (1)
- ALOS World 3D (1)
- APP (1)
- ASTER GDEM (1)
- Adipose tissue (1)
- Adolescents (1)
- Adult-child interaction (1)
- Advection and convection (1)
- Aftercare (1)
- Aging (1)
- Akan (1)
- Akt pathway (1)
- Animal personality (1)
- Anthropologie (1)
- Arabica coffee (1)
- Arabidopsis thaliana (1)
- Bacteria (1)
- Barriers (1)
- Bayesian modelling (1)
- Biomarker (1)
- Brandenburg (1)
- CDOM (1)
- COVID-19 pandemic (1)
- CRISPR editing validation (1)
- Central European Basin System (1)
- Chew Bahir (1)
- Chlamydomonas (1)
- Chronic conditions (1)
- Chronic low back pain (1)
- Cognitive fatigue (1)
- Conversation Analysis (1)
- Copernicus DEM (1)
- Coping (1)
- Cross-frequency PLV (1)
- Cystic fibrosis (1)
- Cytochrome b (1)
- DEM noise (1)
- Damage assessment (1)
- Data pooling (1)
- Delphi study (1)
- Developmental hyperscanning (1)
- Digital Health (1)
- Digital intervention (1)
- Digitalisierung (1)
- Disturbance impacts (1)
- Disturbance indicator (1)
- Dual EEG analysis (1)
- Dynamometry (1)
- EEG (1)
- Eating behavior (1)
- Eccentric muscle action (1)
- Electronic Health (1)
- Elephant disturbance (1)
- Embodied cognition (1)
- Energy expenditure (1)
- Erkenntnisgewinnung (1)
- European hare (1)
- Exertion (1)
- Feature selection (1)
- FieldTrip (1)
- Fluorescence (1)
- Forschungsmethodik (1)
- Forschungsorientierung (1)
- Fortbildung (1)
- Fourier analysis (1)
- Fresh water fish (1)
- GPS (1)
- Gait (1)
- Gene expression (1)
- Genetics (1)
- Geothermal field (1)
- Germany (1)
- Glucose homeostasis (1)
- Governance (1)
- Grip force (1)
- H2S biosynthesis (1)
- HDL (1)
- HIV (1)
- Hamstring-quadriceps ratio (1)
- Handlungsempfehlungen (1)
- Health insurance (1)
- Heat transport by conduction (1)
- Hippo signaling (1)
- Human physical conditioning (1)
- ICDP (1)
- Individualized therapy (1)
- Injury (1)
- Injury risk (1)
- Inklusion (1)
- Insulin resistance (1)
- Inter-individual differences (1)
- Interactional Linguistics (1)
- Internet of Things (1)
- Interoception (1)
- Intuitive eating (1)
- Iron Age (1)
- Irradiation (1)
- Island biogeography (1)
- Jurkat cells (1)
- Just so stories (1)
- Juvenile idiopathic arthritis (1)
- KI-Anwendung (1)
- Klimapolitik (1)
- Kommune (1)
- Künstliche Intelligenz (1)
- K−12 teachers (1)
- LC-MS/MS (1)
- LC/HRMS (1)
- LCSM (1)
- LC–MS/MS (1)
- LDL (1)
- Lake Holzmaar (1)
- Lake Malawi (1)
- Lehramtsstudium Biologie (1)
- Levy walks (1)
- Locally structured correlation (1)
- Locally structured standard deviation (1)
- M1/M2 differentiation (1)
- MALDI-TOF-MS (1)
- MCI (1)
- MSAP (1)
- Mandarin-English bilinguals (1)
- Martial arts (1)
- Maschinelles Lernen (1)
- Matthew effect (1)
- Mental number line (1)
- Metabolic syndrome (1)
- MiSpEx (1)
- MiSpEx-network (1)
- Microtus arvalis (1)
- Mindfulness (1)
- Mitochondria (1)
- Mixed methods (1)
- Mobile Health (1)
- Mobile diagnostics (1)
- Moco biosynthesis (1)
- Monte Carlo method (1)
- Monte-Carlo simulation (1)
- Motor system (1)
- Movement (1)
- Movement ecology (1)
- Muscle strength (1)
- Musculoskeletal and neural physiological phenomena (1)
- N400 (1)
- NAFLD/MAFLD (1)
- Natural Language Processing (1)
- Non-invasive vagus nerve stimulation (1)
- Noradrenaline (1)
- Number processing (1)
- NutriAct Family Study (1)
- Nutrition (1)
- ODBA (1)
- Obesity (1)
- Older adults (1)
- Omicron (1)
- Ontologie (1)
- Overhead athletes (1)
- PLV (1)
- Partial Little Square (1)
- Partnership (1)
- Phase Locking Value (1)
- Philosophie (1)
- Photodynamics (1)
- Phylogeny (1)
- Physical training (1)
- Pipistrellus nathusii (1)
- Plyometric exercise (1)
- Postural control (1)
- Potsdam (1)
- Prevention (1)
- Prior knowledge (1)
- Professionalisierung (1)
- Professionswissen (1)
- Prospective (1)
- Protective factors (1)
- Quality of life (1)
- Quantitative Trait Locus (1)
- Quantitative Trait Locus analysis (1)
- Randomized controlled trial (1)
- Randomized-controlled trial (1)
- Referendariat (1)
- Reflexivität (1)
- Rehabilitation (1)
- Repetition (1)
- Reproducible benchmarking (1)
- Resiliency (1)
- Resistance training (1)
- Rotifera (1)
- Rural health (1)
- SARS-CoV-2 (1)
- SEPE Factors (1)
- SEPS factors (1)
- SFON (1)
- SRT (1)
- SRTM (1)
- Sammelband (1)
- Sedimentary basin (1)
- Selbstreflexion (1)
- Seminarkonzept (1)
- Shire River (1)
- Shoulder (1)
- Social-Economic-Political-Emotional (SEPE) factors (1)
- South-Eastern Africa (1)
- Soziale Medien (1)
- Specific wood density (1)
- Sport-specific performance (1)
- St. Nicolas House Analysis (1)
- Stadt (1)
- Standard deviation (1)
- Statistical Exercise (1)
- Stress response (1)
- Studienabbruch (1)
- Studieneingang (1)
- Studienerfolg (1)
- Sway (1)
- TAVI (1)
- TLR signaling (1)
- TMAO reductase (1)
- TMS (1)
- Tagung (1)
- TanDEM-X (1)
- Telehealth (1)
- Telemedicine (1)
- Theodor W. Adorno (1)
- Tiredness (1)
- Transformation (1)
- Tree allometry (1)
- Twitter (1)
- Type 1 diabetes (1)
- Ulmener Maar Tephra (1)
- Ulrich Sonnemann (1)
- Union (1)
- Unterrichtsanalyse (1)
- Unterrichtsmethoden (1)
- VR (1)
- Variance (1)
- Vignetten (1)
- Wearable electronic device (1)
- Wearable technology (1)
- Wirkungsforschung (1)
- Wood specific gravity (1)
- Woody aboveground biomass (1)
- WorldDEM (1)
- X-ray (1)
- Yamal (1)
- Yap1/Wwtr1 (Taz) (1)
- accelerometer (1)
- acclimation (1)
- acid mine drainage (1)
- acoustic communication (1)
- acyclic diene metathesis (ADMET) polymerization (1)
- adaptive management (1)
- addition (1)
- adipose tissue (1)
- adolescents (1)
- advanced dynamic flow (1)
- aerobic power (1)
- aggression (1)
- aging (1)
- agricultural landscape (1)
- allocation policies (1)
- alpha-2 (1)
- amino acids (1)
- animal migration (1)
- animal personality (1)
- anomalous diffusion (1)
- anterior PNP (1)
- anterior cruciate ligament (1)
- anthropology (1)
- anthropometry (1)
- anti-cancer drugs (1)
- antibody (1)
- antidepressants (1)
- apoptosis (1)
- application of artificial intelligence (1)
- approximate methods (1)
- arable weeds (1)
- artificial intelligence (1)
- asymptotic method (1)
- athletes (1)
- athletic performance (1)
- augmented reality (1)
- automated radio telemetry (1)
- balance (1)
- baroclinicity (1)
- basic need satisfaction and frustration (1)
- bats (1)
- behavior problems (1)
- behavioral and self-report measures (1)
- behavioral type (1)
- behaviour (1)
- bibliometric analysis (1)
- biodiversity conservation (1)
- biological age (1)
- biology (1)
- biomechanics (1)
- birth weight (1)
- bis-MGD (1)
- blockchain (1)
- body mass index (1)
- body proportions (1)
- bound phenolic compounds (1)
- brain health (1)
- breastfeeding (1)
- buffer zones (1)
- burnout (1)
- business process management (1)
- business processes (1)
- cTBS (1)
- callous-unemotional traits (1)
- callousness (1)
- camelid antibody (1)
- camelid heavy-chain-only antibodies (1)
- capabilities (1)
- carbon cycling (1)
- cardiac catheterization (CC) (1)
- cardiac development (1)
- cardiology (1)
- cardiomyopathy (1)
- cardiovascular diseases (1)
- catch-up-growth (1)
- cellular bioenergetics (1)
- cellulose polymeric organic matter (1)
- central and peripheral vision (1)
- cereal leaf beetle (1)
- change of direction speed (1)
- changepoint analysis (1)
- changepoint detection (1)
- chaperone (1)
- child growth (1)
- children and adolescents (1)
- chimera (1)
- cholesterol (1)
- chronic health condition (1)
- chronic undernutrition (1)
- citation analysis (1)
- climate change adaptation (1)
- climate policy (1)
- clinical sample (1)
- coffee processing (1)
- cognitive bias (1)
- cognitive/muscular fatigue (1)
- collegial casework (1)
- color (1)
- community model (1)
- composition (1)
- constraint (1)
- consultation (1)
- content knowledge (1)
- continuing education activities (1)
- copy number analyses (1)
- corpus dataset (1)
- cost optimisation (1)
- counting (1)
- creativity (1)
- cross-species capture (1)
- cyberaggression (1)
- cytosine methylation (1)
- cytosolic tRNA thiolation (1)
- daily home-made measurements (1)
- deep neural networks (1)
- deferred choice (1)
- dental eruption (1)
- depressive disorder (1)
- depressive symptoms (1)
- derivational complexity (1)
- design of experiment (1)
- design thinking (1)
- development (1)
- diabetes mellitus (1)
- diabetic (1)
- digital technologies (1)
- digital transformation (1)
- digitalization (1)
- diketopiperazine (1)
- disaster risk reduction (1)
- discrepancy principle (1)
- disorder recognition (1)
- distress (1)
- drought events (1)
- drought intensity (1)
- drought projections (1)
- drug delivery (1)
- drug-sensitivity prediction (1)
- eavesdropping (1)
- eccentric-plyometric (1)
- echolocation (1)
- economy (1)
- ecophysiology (1)
- ecosystem restoration (1)
- education (1)
- eicosanoids (1)
- elderly (1)
- electrochemistry (1)
- elevated plus-maze (1)
- elf-determination theory (1)
- embodied cognition (1)
- emergency management (1)
- emotional regulation (1)
- emotional stress (1)
- endocardium (1)
- endophytes (1)
- energy expenditure (1)
- energy policy (1)
- enhancement (1)
- entropy (1)
- environmental pollution (1)
- erythropoiesis (1)
- europe (1)
- european (1)
- everyday life (1)
- exercise cognition (1)
- exercise test (1)
- exercise training (1)
- external load (1)
- extracellular enzymes (1)
- extremophiles (1)
- eye movements (1)
- fear (1)
- feature selection (1)
- federated learning (1)
- feedback (1)
- feeling of presence (1)
- fence ecology (1)
- fiction (1)
- finite-size effects (1)
- first-passage time (1)
- first-reaction time (1)
- fitness (1)
- flexibility (1)
- floods (1)
- food access (1)
- food web (1)
- formal semantics (1)
- fractionation (1)
- frailty tool (1)
- frame index (1)
- frames of reference (1)
- freshwater (1)
- frustration (1)
- functional performance (1)
- functional traits (1)
- fungal pathogens (1)
- fungi (1)
- gait (1)
- games (1)
- genome scan (1)
- global change (1)
- governance (1)
- graphs (1)
- ground reaction forces (1)
- growth references (1)
- growth standards (1)
- growth tempo (1)
- guideline (1)
- happiness (1)
- healthcare (1)
- heart rate (1)
- heavy-chain-only antibody (1)
- height in history (1)
- heliozoa (1)
- herbivory (1)
- high density lipoprotein cholesterol (1)
- high-sodium (1)
- hippocampal-prefrontal network (1)
- historical growth (1)
- home range (1)
- homologous recombination deficiency (1)
- homology-directed repair (1)
- hospitalization (1)
- human excised skin (1)
- human physical conditioning (1)
- hybridization capture (1)
- hydrolysis (1)
- iPhone (1)
- imaginary world (1)
- implicit learning (1)
- in-service training (1)
- inclusive education (1)
- injury risk (1)
- innovation laboratories (1)
- inquiry (1)
- insulin (1)
- insulin resistance (1)
- integration (1)
- intellectual ability (1)
- intelligence (1)
- intergroup contacts (1)
- internal load (1)
- intonation units (1)
- intra-organ-communication (1)
- intraguild predation (1)
- ion-exchange chromatography (1)
- kelp (1)
- kinematics (1)
- knots (1)
- kollegiale Fallbesprechung (1)
- lake catchments (1)
- lakes (1)
- landscape transience (1)
- language courses (1)
- linear enamel hypoplasia (1)
- linear sprint (1)
- lipid peroxidation (1)
- lipids (1)
- local governance (1)
- locus coeruleus (1)
- lokale Governance (1)
- long-term effects (1)
- low back pain (1)
- low density lipoprotein cholesterol (1)
- low-back-pain (1)
- macrostructure (1)
- magnitude estimation (1)
- major depressive disorder (1)
- math (1)
- mathematical development (1)
- mathematical precursor (1)
- mcgraph (1)
- measurement (1)
- mechanobiology (1)
- mental health (1)
- mental imagery (1)
- mental number line (1)
- mental simulation (1)
- meta-science (1)
- metabolic disease (1)
- metathesis (1)
- meteorological drought (1)
- methods (1)
- miRNA (1)
- miRNAs (1)
- microfacies (1)
- microplastics (1)
- microstructure (1)
- migrants (1)
- migration (1)
- mild cognitive impairment (1)
- mindfulness-based stress reduction (1)
- mini growth spurt (1)
- mining lakes (1)
- mitochondrial adaptation (1)
- molecular species identification (1)
- molecular weaving (1)
- molybdenum cofactor (1)
- monensin (1)
- mortality (1)
- motor-control-exercise (1)
- movement (1)
- multidisciplinary intervention (1)
- multidisciplinary-therapy (1)
- multiple modalities (1)
- muscle metabolism (1)
- muscle strength (1)
- musculoskeletal and neural physiological phenomena (1)
- museum specimens (1)
- myocardium (1)
- nanobodies (1)
- nanogels (1)
- narrative (1)
- narrative skills (1)
- natural language processing (1)
- natural particle (1)
- nature of science (1)
- need profiles (1)
- neovascularization (1)
- network reconstruction (1)
- networks (1)
- neurological disorders (1)
- neuromuscular (1)
- neutralization (1)
- non-adjacent dependencies (1)
- nonlinear operator (1)
- novel biomarkers (1)
- nucleic acids (1)
- number knowledge (1)
- numerical development (1)
- numerical skills (1)
- nutrition (1)
- nutritional status (1)
- object search (1)
- older patients (1)
- oncology (1)
- ontology (1)
- open-field (1)
- optimal rate (1)
- oracles (1)
- oxidative stress (1)
- oxygen consumption (1)
- pace of life (1)
- paleoclimate (1)
- pandemic (1)
- parentage (1)
- past biosphere (1)
- peak height velocity (1)
- pedagogical content knowledge (PCK) (1)
- pedagogical reasoning (1)
- peptide biomarkers (1)
- performance (1)
- performance evaluation (1)
- periodic entanglement (1)
- permafrost (1)
- philosophy (1)
- phonetics (1)
- phonotaxis (1)
- photoresponse (1)
- photosynthesis (1)
- physical fitness (1)
- physical time (1)
- physiology (1)
- playback (1)
- plyometric exercise (1)
- policy recommendations (1)
- political ideology (1)
- polyenoic fatty acids (1)
- post-natal (1)
- posterior P600 (1)
- pre-natal (1)
- pre-service teacher (1)
- precaution (1)
- precuneus (1)
- predictability (1)
- predictive models (1)
- preschool children (1)
- preschoolers (1)
- prevention (1)
- priming (1)
- privacy and security (1)
- privacy attack (1)
- probabilistic processing (1)
- problem solving (1)
- professional development (1)
- professional knowledge (1)
- professionalization (1)
- prosocial (1)
- prosody (1)
- protein modification (1)
- psycho-oncology (1)
- psychoeducation (1)
- psychopathology (1)
- psychophysiological (1)
- psychotherapy process (1)
- pubertal timing (1)
- public health (1)
- pupil diameter (1)
- purification (1)
- quality (1)
- questioning solutions (1)
- random sampling (1)
- recombinant inbred line (1)
- redox state (1)
- refined consensus model (RCM) (1)
- refugees (1)
- regularization (1)
- regulation of growth (1)
- remote sensing data (1)
- remote teaching (1)
- renewable energy (1)
- repetition (1)
- reproductive success (1)
- research design (1)
- resistance training (1)
- resource-tracking (1)
- response inhibition (1)
- response styles theory (1)
- response time (1)
- resting-state fMRI (1)
- return-to-sport (1)
- reversed-phase chromatography (1)
- review (1)
- rheumatic diseases (1)
- risk management (1)
- role-play (1)
- rotifer (1)
- rule learning (1)
- rumination (1)
- runners (1)
- running mechanics (1)
- rural (1)
- sAA (1)
- scene memorization (1)
- scene viewing (1)
- science teaching (1)
- secular changes (1)
- sedaDNA (1)
- sedentary (1)
- sediment core (1)
- selenite (1)
- self-control (1)
- seminar concept (1)
- sensitivity (1)
- serial reaction time (SRT) task (1)
- shared leadership (1)
- shell-like geometries (1)
- short-term growth (1)
- signal propagation (1)
- single case analysis (1)
- single domain antibodies (1)
- single-case design (1)
- single-case experimental design (1)
- skeletal age (1)
- skin penetration (1)
- smart contracts (1)
- social class (1)
- social environment (1)
- social network (1)
- social status insecurity (1)
- socioeconomy (1)
- solitary bees (1)
- soliton (1)
- sonography (1)
- source-to-sink (1)
- spatial autocorrelation (1)
- spatial frequencies (1)
- spatial language (1)
- spatial updating (1)
- spatially explicit (1)
- special education (1)
- speech (1)
- speed (1)
- sport-specific performance (1)
- sports (1)
- stability (1)
- standardized patient (1)
- standardized patient information (1)
- standards (1)
- standing long jump (1)
- starch granule (1)
- starch granule morphology (1)
- starch granule size (1)
- starch metabolism (1)
- step-growth polymerization (1)
- stochastic resetting (1)
- storm tracks (1)
- stratigraphy (1)
- stress (1)
- stress intervention (1)
- stress management (1)
- striking combat sports (1)
- structural equation model (1)
- study designs (1)
- study protocol (1)
- subtraction (1)
- sulfite oxidase (1)
- sustainability (1)
- syntax (1)
- tVNS (1)
- tacrolimus formulation (1)
- taekwondo electronic scoring system (1)
- taekwondo-specific testing (1)
- talk-in-interaction (1)
- tangles (1)
- target capture (1)
- teacher learning (1)
- teacher quality (1)
- teacher training (1)
- teaching education (1)
- teaching practice (1)
- tendinosis (1)
- therapist competence (1)
- theta (1)
- time-efficient exercise (1)
- toxicity (1)
- training (1)
- training adaptation (1)
- transformation (1)
- transformation products (1)
- translation (1)
- treatment integrity (1)
- triglycerides (1)
- type 2 diabetes (1)
- type specimens (1)
- ultrasound (1)
- uncaring (1)
- unemotional (1)
- ungulate (1)
- usability (1)
- varves (1)
- veterinary cordon fence (1)
- veterinary drugs (1)
- vicious cycle (1)
- vignettes (1)
- virus infection (1)
- voice (1)
- water sports (1)
- web-based (1)
- wh-ex-situ (1)
- wh-in-situ (1)
- wh-questions (1)
- wildlife and habitat management (1)
- workflow patterns (1)
- working memory (1)
- yellow flags (1)
- youth soccer (1)
- youth sport (1)
- youth sports (1)
- zebrafish (1)
- zweite Ausbildungsphase (1)
- α-amylase/trypsin inhibitors (1)
- „Natur der Naturwissenschaften“ (1)
- social network analysis (1)
- team creativity (1)
- intrapreneurship (1)
Institute
- Extern (193) (remove)
Intelligence, as well as working memory and attention, affect the acquisition of mathematical competencies. This paper aimed to examine the influence of working memory and attention when taking different mathematical skills into account as a function of children’s intellectual ability. Overall, intelligence, working memory, attention and numerical skills were assessed twice in 1868 German pre-school children (t1, t2) and again at 2nd grade (t3). We defined three intellectual ability groups based on the results of intellectual assessment at t1 and t2. Group comparisons revealed significant differences between the three intellectual ability groups. Over time, children with low intellectual ability showed the lowest achievement in domain-general and numerical and mathematical skills compared to children of average intellectual ability. The highest achievement on the aforementioned variables was found for children of high intellectual ability. Additionally, path modelling revealed that, depending on the intellectual ability, different models of varying complexity could be generated. These models differed with regard to the relevance of the predictors (t2) and the future mathematical skills (t3). Causes and conclusions of these findings are discussed.
In this paper we examine the effect of uncertainty on readers’ predictions about meaning. In particular, we were interested in how uncertainty might influence the likelihood of committing to a specific sentence meaning. We conducted two event-related potential (ERP) experiments using particle verbs such as turn down and manipulated uncertainty by constraining the context such that readers could be either highly certain about the identity of a distant verb particle, such as turn the bed […] down, or less certain due to competing particles, such as turn the music […] up/down. The study was conducted in German, where verb particles appear clause-finally and may be separated from the verb by a large amount of material. We hypothesised that this separation would encourage readers to predict the particle, and that high certainty would make prediction of a specific particle more likely than lower certainty. If a specific particle was predicted, this would reflect a strong commitment to sentence meaning that should incur a higher processing cost if the prediction is wrong. If a specific particle was less likely to be predicted, commitment should be weaker and the processing cost of a wrong prediction lower. If true, this could suggest that uncertainty discourages predictions via an unacceptable cost-benefit ratio. However, given the clear predictions made by the literature, it was surprisingly unclear whether the uncertainty manipulation affected the two ERP components studied, the N400 and the PNP. Bayes factor analyses showed that evidence for our a priori hypothesised effect sizes was inconclusive, although there was decisive evidence against a priori hypothesised effect sizes larger than 1μV for the N400 and larger than 3μV for the PNP. We attribute the inconclusive finding to the properties of verb-particle dependencies that differ from the verb-noun dependencies in which the N400 and PNP are often studied.
Injuries in professional soccer are a significant concern for teams, and they are caused amongst others by high training load. This cohort study describes the relationship between workload parameters and the occurrence of non-contact injuries, during weeks with high and low workload in professional soccer players throughout the season. Twenty-one professional soccer players aged 28.3 ± 3.9 yrs. who competed in the Iranian Persian Gulf Pro League participated in this 48-week study. The external load was monitored using global positioning system (GPS, GPSPORTS Systems Pty Ltd) and the type of injury was documented daily by the team's medical staff. Odds ratio (OR) and relative risk (RR) were calculated for non-contact injuries for high- and low-load weeks according to acute (AW), chronic (CW), acute to chronic workload ratio (ACWR), and AW variation (Δ-Acute) values. By using Poisson distribution, the interval between previous and new injuries were estimated. Overall, 12 non-contact injuries occurred during high load and 9 during low load weeks. Based on the variables ACWR and Δ-AW, there was a significantly increased risk of sustaining non-contact injuries (p < 0.05) during high-load weeks for ACWR (OR: 4.67), and Δ-AW (OR: 4.07). Finally, the expected time between injuries was significantly shorter in high load weeks for ACWR [1.25 vs. 3.33, rate ratio time (RRT)] and Δ-AW (1.33 vs. 3.45, RRT) respectively, compared to low load weeks. The risk of sustaining injuries was significantly larger during high workload weeks for ACWR, and Δ-AW compared with low workload weeks. The observed high OR in high load weeks indicate that there is a significant relationship between workload and occurrence of non-contact injuries. The predicted time to new injuries is shorter in high load weeks compared to low load weeks. Therefore, the frequency of injuries is higher during high load weeks for ACWR and Δ-AW. ACWR and Δ-AW appear to be good indicators for estimating the injury risk, and the time interval between injuries.
In order to improve a recently established cell-based assay to assess the potency of botulinum neurotoxin, neuroblastoma-derived SiMa cells and induced pluripotent stem-cells (iPSC) were modified to incorporate the coding sequence of a reporter luciferase into a genetic safe harbor utilizing CRISPR/Cas9. A novel method, the double-control quantitative copy number PCR (dc-qcnPCR), was developed to detect off-target integrations of donor DNA. The donor DNA insertion success rate and targeted insertion success rate were analyzed in clones of each cell type. The dc-qcnPCR reliably quantified the copy number in both cell lines. The probability of incorrect donor DNA integration was significantly increased in SiMa cells in comparison to the iPSCs. This can possibly be explained by the lower bundled relative gene expression of a number of double-strand repair genes (BRCA1, DNA2, EXO1, MCPH1, MRE11, and RAD51) in SiMa clones than in iPSC clones. The dc-qcnPCR offers an efficient and cost-effective method to detect off-target CRISPR/Cas9-induced donor DNA integrations.
The role of the GMP nucleotides of the bis-molybdopterin guanine dinucleotide (bis-MGD) cofactor of the DMSO reductase family has long been a subject of discussion. The recent characterization of the bis-molybdopterin (bis-Mo-MPT) cofactor present in the E. coli YdhV protein, which differs from bis-MGD solely by the absence of the nucleotides, now enables studying the role of the nucleotides of bis-MGD and bis-MPT cofactors in Moco insertion and the activity of molybdoenzymes in direct comparison. Using the well-known E. coli TMAO reductase TorA as a model enzyme for cofactor insertion, we were able to show that the GMP nucleotides of bis-MGD are crucial for the insertion of the bis-MGD cofactor into apo-TorA.
The intake of high-fat diets (HFDs) containing large amounts of saturated long-chain fatty acids leads to obesity, oxidative stress, inflammation, and insulin resistance. The trace element selenium, as a crucial part of antioxidative selenoproteins, can protect against the development of diet-induced insulin resistance in white adipose tissue (WAT) by increasing glutathione peroxidase 3 (GPx3) and insulin receptor (IR) expression. Whether selenite (Se) can attenuate insulin resistance in established lipotoxic and obese conditions is unclear. We confirm that GPX3 mRNA expression in adipose tissue correlates with BMI in humans. Cultivating 3T3-L1 pre-adipocytes in palmitate-containing medium followed by Se treatment attenuates insulin resistance with enhanced GPx3 and IR expression and adipocyte differentiation. However, feeding obese mice a selenium-enriched high-fat diet (SRHFD) only resulted in a modest increase in overall selenoprotein gene expression in WAT in mice with unaltered body weight development, glucose tolerance, and insulin resistance. While Se supplementation improved adipocyte morphology, it did not alter WAT insulin sensitivity. However, mice fed a SRHFD exhibited increased insulin content in the pancreas. Overall, while selenite protects against palmitate-induced insulin resistance in vitro, obesity impedes the effect of selenite on insulin action and adipose tissue metabolism in vivo.
The Covid-19 pandemic imposed new constraints on empirical research and forced researchers to transfer from traditional laboratory research to the online environment. This study tested the validity of a web-based episodic memory paradigm by comparing participants’ memory performance for trustworthy and untrustworthy facial stimuli in a supervised laboratory setting and an unsupervised web setting. Consistent with previous results, we observed enhanced episodic memory for untrustworthy compared to trustworthy faces. Most importantly, this memory bias was comparable in the online and the laboratory experiment, suggesting that web-based procedures are a promising tool for memory research.
Cognitive resources contribute to balance control. There is evidence that mental fatigue reduces cognitive resources and impairs balance performance, particularly in older adults and when balance tasks are complex, for example when trying to walk or stand while concurrently performing a secondary cognitive task.
We conducted a systematic literature search in PubMed (MEDLINE), Web of Science and Google Scholar to identify eligible studies and performed a random effects meta-analysis to quantify the effects of experimentally induced mental fatigue on balance performance in healthy adults. Subgroup analyses were computed for age (healthy young vs. healthy older adults) and balance task complexity (balance tasks with high complexity vs. balance tasks with low complexity) to examine the moderating effects of these factors on fatigue-mediated balance performance.
We identified 7 eligible studies with 9 study groups and 206 participants. Analysis revealed that performing a prolonged cognitive task had a small but significant effect (SMDwm = −0.38) on subsequent balance performance in healthy young and older adults. However, age- and task-related differences in balance responses to fatigue could not be confirmed statistically.
Overall, aggregation of the available literature indicates that mental fatigue generally reduces balance in healthy adults. However, interactions between cognitive resource reduction, aging and balance task complexity remain elusive.
How do different reset protocols affect ergodicity of a diffusion process in single-particle-tracking experiments? We here address the problem of resetting of an arbitrary stochastic anomalous-diffusion process (ADP) from the general mathematical points of view and assess ergodicity of such reset ADPs for an arbitrary resetting protocol. The process of stochastic resetting describes the events of the instantaneous restart of a particle’s motion via randomly distributed returns to a preset initial position (or a set of those). The waiting times of such resetting events obey the Poissonian, Gamma, or more generic distributions with specified conditions regarding the existence of moments. Within these general approaches, we derive general analytical results and support them by computer simulations for the behavior of the reset mean-squared displacement (MSD), the new reset increment-MSD (iMSD), and the mean reset time-averaged MSD (TAMSD). For parental nonreset ADPs with the MSD(t)∝ tμ we find a generic behavior and a switch of the short-time growth of the reset iMSD and mean reset TAMSDs from ∝ _μ for subdiffusive to ∝ _1 for superdiffusive reset ADPs. The critical condition for a reset ADP that recovers its ergodicity is found to be more general than that for the nonequilibrium stationary state, where obviously the iMSD and the mean TAMSD are equal. The consideration of the new statistical quantifier, the iMSD—as compared to the standard MSD—restores the ergodicity of an arbitrary reset ADP in all situations when the μth moment of the waiting-time distribution of resetting events is finite. Potential applications of these new resetting results are, inter alia, in the area of biophysical and soft-matter systems.
There is a large variety of goals instructors have for laboratory courses, with different courses focusing on different subsets of goals. An often implicit, but crucial, goal is to develop students’ attitudes, views, and expectations about experimental physics to align with practicing experimental physicists. The assessment of laboratory courses upon this one dimension of learning has been intensively studied in U.S. institutions using the Colorado Learning Attitudes about Science Survey for Experimental Physics (E-CLASS). However, there is no such an instrument available to use in Germany, and the influence of laboratory courses on students views about the nature of experimental physics is still unexplored at German-speaking institutions. Motivated by the lack of an assessment tool to investigate this goal in laboratory courses at German-speaking institutions, we present a translated version of the E-CLASS adapted to the context at German-speaking institutions. We call the German version of the E-CLASS, the GE-CLASS. We describe the translation process and the creation of an automated web-based system for instructors to assess their laboratory courses. We also present first results using GE-CLASS obtained at the University of Potsdam. A first comparison between E-CLASS and GE-CLASS results shows clear differences between University of Potsdam and U.S. students’ views and beliefs about experimental physics.
We introduce and study a Lévy walk (LW) model of particle spreading with a finite propagation speed combined with soft resets, stochastically occurring periods in which an harmonic external potential is switched on and forces the particle towards a specific position. Soft resets avoid instantaneous relocation of particles that in certain physical settings may be considered unphysical. Moreover, soft resets do not have a specific resetting point but lead the particle towards a resetting point by a restoring Hookean force. Depending on the exact choice for the LW waiting time density and the probability density of the periods when the harmonic potential is switched on, we demonstrate a rich emerging response behaviour including ballistic motion and superdiffusion. When the confinement periods of the soft-reset events are dominant, we observe a particle localisation with an associated non-equilibrium steady state. In this case the stationary particle probability density function turns out to acquire multimodal states. Our derivations are based on Markov chain ideas and LWs with multiple internal states, an approach that may be useful and flexible for the investigation of other generalised random walks with soft and hard resets. The spreading efficiency of soft-rest LWs is characterised by the first-passage time statistic.
Background:
Children’s spontaneous focusing on numerosity (SFON) is related to numerical skills. This study aimed to examine (1) the developmental trajectory of SFON and (2) the interrelations between SFON and early numerical skills at pre-school as well as their influence on arithmetical skills at school.
Method:
Overall, 1868 German pre-school children were repeatedly assessed until second grade. Nonverbal intelligence, visual attention, visuospatial working memory, SFON and numerical skills were assessed at age five (M = 63 months, Time 1) and age six (M = 72 months, Time 2), and arithmetic was assessed at second grade (M = 95 months, Time 3).
Results:
SFON increased significantly during pre-school. Path analyses revealed interrelations between SFON and several numerical skills, except number knowledge. Magnitude estimation and basic calculation skills (Time 1 and Time 2), and to a small degree number knowledge (Time 2), contributed directly to arithmetic in second grade. The connection between SFON and arithmetic was fully mediated by magnitude estimation and calculation skills at pre-school.
Conclusion:
Our results indicate that SFON first and foremost influences deeper understanding of numerical concepts at pre-school and—in contrast to previous findings –affects only indirectly children’s arithmetical development at school.
Studies have revealed mixed results on how antidepressant drugs affect lipid profiles of patients with major depression disorder (MDD). Even less is known about how patients respond to a switch of antidepressant medication with respect to their metabolic profile. For this, effects of a switch in antidepressants medication on lipid markers were studied in MDD patients. 15 participants (females = 86.67%; males = 13.33%; age: 49.45 ± 7.45 years) with MDD and a prescribed switch in their antidepressant medication were recruited at a psychosomatic rehabilitation clinic. Participants were characterized (with questionnaires and blood samples) at admission to the rehabilitation clinic (baseline, T0) and followed up with a blood sample two weeks (T1) later. HDL, LDL, total cholesterol, and triglycerides were determined (T0), and their change analyzed (Wilcoxon test) at follow up (T1). Decrements in HDL (p = 0.041), LDL (p < 0.001), and total cholesterol (p < 0.001) were observed two weeks after a switch in antidepressant medication. Triglycerides showed no difference (p = 0.699). Overall, LDL, HDL, and total cholesterol are affected by a change in antidepressant drugs in patients with MDD. These observations are of clinical relevance for medical practitioners in the planning and management of treatment strategies for MDD patients.
Wheat alpha-amylase/trypsin inhibitors remain a subject of interest considering the latest findings showing their implication in wheat-related non-celiac sensitivity (NCWS). Understanding their functions in such a disorder is still unclear and for further study, the need for pure ATI molecules is one of the limiting problems. In this work, a simplified approach based on the successive fractionation of ATI extracts by reverse phase and ion exchange chromatography was developed. ATIs were first extracted from wheat flour using a combination of Tris buffer and chloroform/methanol methods. The separation of the extracts on a C18 column generated two main fractions of interest F1 and F2. The response surface methodology with the Doehlert design allowed optimizing the operating parameters of the strong anion exchange chromatography. Finally, the seven major wheat ATIs namely P01083, P17314, P16850, P01085, P16851, P16159, and P83207 were recovered with purity levels (according to the targeted LC-MS/MS analysis) of 98.2 ± 0.7; 98.1 ± 0.8; 97.9 ± 0.5; 95.1 ± 0.8; 98.3 ± 0.4; 96.9 ± 0.5, and 96.2 ± 0.4%, respectively. MALDI-TOF-MS analysis revealed single peaks in each of the pure fractions and the mass analysis yielded deviations of 0.4, 1.9, 0.1, 0.2, 0.2, 0.9, and 0.1% between the theoretical and the determined masses of P01083, P17314, P16850, P01085, P16851, P16159, and P83207, respectively. Overall, the study allowed establishing an efficient purification process of the most important wheat ATIs. This paves the way for further in-depth investigation of the ATIs to gain more knowledge related to their involvement in NCWS disease and to allow the absolute quantification in wheat samples.
Older adults with amnestic mild cognitive impairment (aMCI) who in addition to their memory deficits also suffer from frontal-executive dysfunctions have a higher risk of developing dementia later in their lives than older adults with aMCI without executive deficits and older adults with non-amnestic MCI (naMCI). Handgrip strength (HGS) is also correlated with the risk of cognitive decline in the elderly. Hence, the current study aimed to investigate the associations between HGS and executive functioning in individuals with aMCI, naMCI and healthy controls. Older, right-handed adults with amnestic MCI (aMCI), non-amnestic MCI (naMCI), and healthy controls (HC) conducted a handgrip strength measurement via a handheld dynamometer. Executive functions were assessed with the Trail Making Test (TMT A&B). Normalized handgrip strength (nHGS, normalized to Body Mass Index (BMI)) was calculated and its associations with executive functions (operationalized through z-scores of TMT B/A ratio) were investigated through partial correlation analyses (i.e., accounting for age, sex, and severity of depressive symptoms). A positive and low-to-moderate correlation between right nHGS (rp (22) = 0.364; p = 0.063) and left nHGS (rp (22) = 0.420; p = 0.037) and executive functioning in older adults with aMCI but not in naMCI or HC was observed. Our results suggest that higher levels of nHGS are linked to better executive functioning in aMCI but not naMCI and HC. This relationship is perhaps driven by alterations in the integrity of the hippocampal-prefrontal network occurring in older adults with aMCI. Further research is needed to provide empirical evidence for this assumption.
We collect a network dataset of tenured economics faculty in Austria, Germany and Switzerland. We rank the 100 institutions included with a minimum violation ranking. This ranking is positively and significantly correlated with the Times Higher Education ranking of economics institutions. According to the network ranking, individuals on average go down about 23 ranks from their doctoral institution to their employing institution. While the share of females in our dataset is only 15%, we do not observe a significant gender hiring gap (a difference in rank changes between male and female faculty). We conduct a robustness check with the Handelsblatt and the Times Higher Education ranking. According to these rankings, individuals on average go down only about two ranks. We do not observe a significant gender hiring gap using these two rankings (although the dataset underlying this analysis is small and these estimates are likely to be noisy). Finally, we discuss the limitations of the network ranking in our context.
Fitness, risk taking, and spatial behavior covary with boldness in experimental vole populations
(2022)
Individuals of a population may vary along a pace-of-life syndrome from highly fecund, short-lived, bold, dispersive “fast” types at one end of the spectrum to less fecund, long-lived, shy, plastic “slow” types at the other end. Risk-taking behavior might mediate the underlying life history trade-off, but empirical evidence supporting this hypothesis is still ambiguous. Using experimentally created populations of common voles (Microtus arvalis)—a species with distinct seasonal life history trajectories—we aimed to test whether individual differences in boldness behavior covary with risk taking, space use, and fitness. We quantified risk taking, space use (via automated tracking), survival, and reproductive success (via genetic parentage analysis) in 8 to 14 experimental, mixed-sex populations of 113 common voles of known boldness type in large grassland enclosures over a significant part of their adult life span and two reproductive events. Populations were assorted to contain extreme boldness types (bold or shy) of both sexes. Bolder individuals took more risks than shyer ones, which did not affect survival. Bolder males but not females produced more offspring than shy conspecifics. Daily home range and core area sizes, based on 95% and 50% Kernel density estimates (20 ± 10 per individual, n = 54 individuals), were highly repeatable over time. Individual space use unfolded differently for sex-boldness type combinations over the course of the experiment. While day ranges decreased for shy females, they increased for bold females and all males. Space use trajectories may, hence, indicate differences in coping styles when confronted with a novel social and physical environment. Thus, interindividual differences in boldness predict risk taking under near-natural conditions and have consequences for fitness in males, which have a higher reproductive potential than females. Given extreme inter- and intra-annual fluctuations in population density in the study species and its short life span, density-dependent fluctuating selection operating differently on the sexes might maintain (co)variation in boldness, risk taking, and pace-of-life.
The protein fraction, important for coffee cup quality, is modified during post-harvest treatment prior to roasting. Proteins may interact with phenolic compounds, which constitute the major metabolites of coffee, where the processing affects these interactions. This allows the hypothesis that the proteins are denatured and modified via enzymatic and/or redox activation steps. The present study was initiated to encompass changes in the protein fraction. The investigations were limited to major storage protein of green coffee beans. Fourteen Coffea arabica samples from various processing methods and countries were used. Different extraction protocols were compared to maintain the status quo of the protein modification. The extracts contained about 4–8 µg of chlorogenic acid derivatives per mg of extracted protein. High-resolution chromatography with multiple reaction monitoring was used to detect lysine modifications in the coffee protein. Marker peptides were allocated for the storage protein of the coffee beans. Among these, the modified peptides K.FFLANGPQQGGK.E and R.LGGK.T of the α-chain and R.ITTVNSQK.I and K.VFDDEVK.Q of β-chain were detected. Results showed a significant increase (p < 0.05) of modified peptides from wet processed green beans as compared to the dry ones. The present study contributes to a better understanding of the influence of the different processing methods on protein quality and its role in the scope of coffee cup quality and aroma. View Full-Text
We study the probability density function (PDF) of the first-reaction times between a diffusive ligand and a membrane-bound, immobile imperfect target region in a restricted 'onion-shell' geometry bounded by two nested membranes of arbitrary shapes. For such a setting, encountered in diverse molecular signal transduction pathways or in the narrow escape problem with additional steric constraints, we derive an exact spectral form of the PDF, as well as present its approximate form calculated by help of the so-called self-consistent approximation. For a particular case when the nested domains are concentric spheres, we get a fully explicit form of the approximated PDF, assess the accuracy of this approximation, and discuss various facets of the obtained distributions. Our results can be straightforwardly applied to describe the PDF of the terminal reaction event in multi-stage signal transduction processes.
High-salt (HS) diets have recently been linked to oxidative stress in the brain, a fact that may be a precursor to behavioral changes, such as those involving anxiety-like behavior. However, to the best of our knowledge, no study has evaluated the amygdala redox status after consuming a HS diet in the pre- or postweaning periods. This study aimed to evaluate the amygdala redox status and anxiety-like behaviors in adulthood, after inclusion of HS diet in two periods: preconception, gestation, and lactation (preweaning); and only after weaning (postweaning). Initially, 18 females and 9 male Wistar rats received a standard (n = 9 females and 4 males) or a HS diet (n = 9 females and 5 males) for 120 days. After mating, females continued to receive the aforementioned diets during gestation and lactation. Weaning occurred at 21-day-old Wistar rats and the male offspring were subdivided: control-control (C-C)—offspring of standard diet fed dams who received a standard diet after weaning (n = 9–11), control-HS (C-HS)—offspring of standard diet fed dams who received a HS diet after weaning (n = 9–11), HS-C—offspring of HS diet fed dams who received a standard diet after weaning (n = 9–11), and HS-HS—offspring of HS diet fed dams who received a HS diet after weaning (n = 9–11). At adulthood, the male offspring performed the elevated plus maze and open field tests. At 152-day-old Wistar rats, the offspring were euthanized and the amygdala was removed for redox state analysis. The HS-HS group showed higher locomotion and rearing frequency in the open field test. These results indicate that this group developed hyperactivity. The C-HS group had a higher ratio of entries and time spent in the open arms of the elevated plus maze test in addition to a higher head-dipping frequency. These results suggest less anxiety-like behaviors. In the analysis of the redox state, less activity of antioxidant enzymes and higher levels of the thiobarbituric acid reactive substances (TBARS) in the amygdala were shown in the amygdala of animals that received a high-salt diet regardless of the period (pre- or postweaning). In conclusion, the high-salt diet promoted hyperactivity when administered in the pre- and postweaning periods. In animals that received only in the postweaning period, the addition of salt induced a reduction in anxiety-like behaviors. Also, regardless of the period, salt provided amygdala oxidative stress, which may be linked to the observed behaviors.
Mediterranean ecosystems are particularly vulnerable to climate change and the associated increase in climate anomalies. This study investigates extreme ecosystem responses evoked by climatic drivers in the Mediterranean Basin for the time span 1999–2019 with a specific focus on seasonal variations as the seasonal timing of climatic anomalies is considered essential for impact and vulnerability assessment. A bivariate vulnerability analysis is performed for each month of the year to quantify which combinations of the drivers temperature (obtained from ERA5-Land) and soil moisture (obtained from ESA CCI and ERA5-Land) lead to extreme reductions in ecosystem productivity using the fraction of absorbed photosynthetically active radiation (FAPAR; obtained from the Copernicus Global Land Service) as a proxy.
The bivariate analysis clearly showed that, in many cases, it is not just one but a combination of both drivers that causes ecosystem vulnerability. The overall pattern shows that Mediterranean ecosystems are prone to three soil moisture regimes during the yearly cycle: they are vulnerable to hot and dry conditions from May to July, to cold and dry conditions from August to October, and to cold conditions from November to April, illustrating the shift from a soil-moisture-limited regime in summer to an energy-limited regime in winter. In late spring, a month with significant vulnerability to hot conditions only often precedes the next stage of vulnerability to both hot and dry conditions, suggesting that high temperatures lead to critically low soil moisture levels with a certain time lag. In the eastern Mediterranean, the period of vulnerability to hot and dry conditions within the year is much longer than in the western Mediterranean. Our results show that it is crucial to account for both spatial and temporal variability to adequately assess ecosystem vulnerability. The seasonal vulnerability approach presented in this study helps to provide detailed insights regarding the specific phenological stage of the year in which ecosystem vulnerability to a certain climatic condition occurs.
How to cite.
Vogel, J., Paton, E., and Aich, V.: Seasonal ecosystem vulnerability to climatic anomalies in the Mediterranean, Biogeosciences, 18, 5903–5927, https://doi.org/10.5194/bg-18-5903-2021, 2021.
Background
Millions of people in Germany suffer from chronic pain, in which course and intensity are multifactorial. Besides physical injuries, certain psychosocial risk factors are involved in the disease process. The national health care guidelines for the diagnosis and treatment of non-specific low back pain recommend the screening of psychosocial risk factors as early as possible, to be able to adapt the therapy to patient needs (e.g., unimodal or multimodal). However, such a procedure has been difficult to implement in practice and has not yet been integrated into the rehabilitation care structures across the country.
Methods
The aim of this study is to implement an individualized therapy and aftercare program within the rehabilitation offer of the German Pension Insurance in the area of orthopedics and to examine its success and sustainability in comparison to the previous standard aftercare program.
The study is a multicenter randomized controlled trial including 1204 patients from six orthopedic rehabilitation clinics. A 2:1 allocation ratio to intervention (individualized and home-based rehabilitation aftercare) versus the control group (regular outpatient rehabilitation aftercare) is set. Upon admission to the rehabilitation clinic, participants in the intervention group will be screened according to their psychosocial risk profile. They could then receive either unimodal or multimodal, together with an individualized training program. The program is instructed in the clinic (approximately 3 weeks) and will continue independently at home afterwards for 3 months. The success of the program is examined by means of a total of four surveys. The co-primary outcomes are the Characteristic Pain Intensity and Disability Score assessed by the German version of the Chronic Pain Grade questionnaire (CPG).
Discussion
An improvement in terms of pain, work ability, patient compliance, and acceptance in our intervention program compared to the standard aftercare is expected. The study contributes to provide individualized care also to patients living far away from clinical centers.
Trial registration
DRKS, DRKS00020373. Registered on 15 April 2020
Recreational exercising and self-reported cardiometabolic diseases in German people living with HIV
(2021)
Exercise is known for its beneficial effects on preventing cardiometabolic diseases (CMDs) in the general population. People living with the human immunodeficiency virus (PLWH) are prone to sedentarism, thus raising their already elevated risk of developing CMDs in comparison to individuals without HIV. The aim of this cross-sectional study was to determine if exercise is associated with reduced risk of self-reported CMDs in a German HIV-positive sample (n = 446). Participants completed a self-report survey to assess exercise levels, date of HIV diagnosis, CD4 cell count, antiretroviral therapy, and CMDs. Participants were classified into exercising or sedentary conditions. Generalized linear models with Poisson regression were conducted to assess the prevalence ratio (PR) of PLWH reporting a CMD. Exercising PLWH were less likely to report a heart arrhythmia for every increase in exercise duration (PR: 0.20: 95% CI: 0.10–0.62, p < 0.01) and diabetes mellitus for every increase in exercise session per week (PR: 0.40: 95% CI: 0.10–1, p < 0.01). Exercise frequency and duration are associated with a decreased risk of reporting arrhythmia and diabetes mellitus in PLWH. Further studies are needed to elucidate the mechanisms underlying exercise as a protective factor for CMDs in PLWH.
Infants show impressive speech decoding abilities and detect acoustic regularities that highlight the syntactic relations of a language, often coded via non-adjacent dependencies (NADs, e.g., is singing). It has been claimed that infants learn NADs implicitly and associatively through passive listening and that there is a shift from effortless associative learning to a more controlled learning of NADs after the age of 2 years, potentially driven by the maturation of the prefrontal cortex. To investigate if older children are able to learn NADs, Lammertink et al. (2019) recently developed a word-monitoring serial reaction time (SRT) task and could show that 6–11-year-old children learned the NADs, as their reaction times (RTs) increased then they were presented with violated NADs. In the current study we adapted their experimental paradigm and tested NAD learning in a younger group of 52 children between the age of 4–8 years in a remote, web-based, game-like setting (whack-a-mole). Children were exposed to Italian phrases containing NADs and had to monitor the occurrence of a target syllable, which was the second element of the NAD. After exposure, children did a “Stem Completion” task in which they were presented with the first element of the NAD and had to choose the second element of the NAD to complete the stimuli. Our findings show that, despite large variability in the data, children aged 4–8 years are sensitive to NADs; they show the expected differences in r RTs in the SRT task and could transfer the NAD-rule in the Stem Completion task. We discuss these results with respect to the development of NAD dependency learning in childhood and the practical impact and limitations of collecting these data in a web-based setting.
Which event happened first?
(2021)
First come, first served: Critical choices between alternative actions are often made based on events external to an organization, and reacting promptly to their occurrence can be a major advantage over the competition. In Business Process Management (BPM), such deferred choices can be expressed in process models, and they are an important aspect of process engines. Blockchain-based process execution approaches are no exception to this, but are severely limited by the inherent properties of the platform: The isolated environment prevents direct access to external entities and data, and the non-continual runtime based entirely on atomic transactions impedes the monitoring and detection of events. In this paper we provide an in-depth examination of the semantics of deferred choice, and transfer them to environments such as the blockchain. We introduce and compare several oracle architectures able to satisfy certain requirements, and show that they can be implemented using state-of-the-art blockchain technology.
Soziale Medien sind ein wesentlicher Bestandteil des Alltags von Schüler*innen und gleichzeitig zunehmend wichtig in Wirtschaft, Politik und Wissenschaft. Am Beispiel von Twitter zeigt dieser Beitrag, dass soziale Medien im Unterricht auch für die Beantwortung geographischer Fragestellungen verwendet werden können. Hierfür eignen sich Twitter-Daten aufgrund ihrer Georeferenzierung und weiterer interessanter Inhalte besonders. Der Beitrag gibt einen Überblick über die Verwendung von Twitter für sozialwissenschaftliche und humangeographische Fragestellungen und reflektiert die Nutzung von Twitter im Unterricht. Für die Unterrichtspraxis werden Beispiele zu den Themen Braunkohle, Flutereignisse und Raumwahrnehmungen sowie Anleitungen zur Auswertung, Anwendung und Reflexion von Twitter-Analysen vorgestellt.
Cellulose and chitin are the most abundant polymeric, organic carbon source globally. Thus, microbes degrading these polymers significantly influence global carbon cycling and greenhouse gas production. Fungi are recognized as important for cellulose decomposition in terrestrial environments, but are far less studied in marine environments, where bacterial organic matter degradation pathways tend to receive more attention. In this study, we investigated the potential of fungi to degrade kelp detritus, which is a major source of cellulose in marine systems. Given that kelp detritus can be transported considerable distances in the marine environment, we were specifically interested in the capability of endophytic fungi, which are transported with detritus, to ultimately contribute to kelp detritus degradation. We isolated 10 species and two strains of endophytic fungi from the kelp Ecklonia radiata. We then used a dye decolorization assay to assess their ability to degrade organic polymers (lignin, cellulose, and hemicellulose) under both oxic and anoxic conditions and compared their degradation ability with common terrestrial fungi. Under oxic conditions, there was evidence that Ascomycota isolates produced cellulose-degrading extracellular enzymes (associated with manganese peroxidase and sulfur-containing lignin peroxidase), while Mucoromycota isolates appeared to produce both lignin and cellulose-degrading extracellular enzymes, and all Basidiomycota isolates produced lignin-degrading enzymes (associated with laccase and lignin peroxidase). Under anoxic conditions, only three kelp endophytes degraded cellulose. We concluded that kelp fungal endophytes can contribute to cellulose degradation in both oxic and anoxic environments. Thus, endophytic kelp fungi may play a significant role in marine carbon cycling via polymeric organic matter degradation.
Frailty assessment is recommended before elective transcatheter aortic valve implantation (TAVI) to determine post-interventional prognosis. Several studies have investigated frailty in TAVI-patients using numerous assessments; however, it remains unclear which is the most appropriate tool for clinical practice. Therefore, we evaluate which frailty assessment is mainly used and meaningful for ≤30-day and ≥1-year prognosis in TAVI patients. Randomized controlled or observational studies (prospective/retrospective) investigating all-cause mortality in older (≥70 years) TAVI patients were identified (PubMed; May 2020). In total, 79 studies investigating frailty with 49 different assessments were included. As single markers of frailty, mostly gait speed (23 studies) and serum albumin (16 studies) were used. Higher risk of 1-year mortality was predicted by slower gait speed (highest Hazard Ratios (HR): 14.71; 95% confidence interval (CI) 6.50–33.30) and lower serum albumin level (highest HR: 3.12; 95% CI 1.80–5.42). Composite indices (five items; seven studies) were associated with 30-day (highest Odds Ratio (OR): 15.30; 95% CI 2.71–86.10) and 1-year mortality (highest OR: 2.75; 95% CI 1.55–4.87). In conclusion, single markers of frailty, in particular gait speed, were widely used to predict 1-year mortality. Composite indices were appropriate, as well as a comprehensive assessment of frailty. View Full-Text
Background:
Research into the application of virtual reality technology in the health care sector has rapidly increased, resulting in a large body of research that is difficult to keep up with.
Objective:
We will provide an overview of the annual publication numbers in this field and the most productive and influential countries, journals, and authors, as well as the most used, most co-occurring, and most recent keywords.
Methods:
Based on a data set of 356 publications and 20,363 citations derived from Web of Science, we conducted a bibliometric analysis using BibExcel, HistCite, and VOSviewer.
Results:
The strongest growth in publications occurred in 2020, accounting for 29.49% of all publications so far. The most productive countries are the United States, the United Kingdom, and Spain; the most influential countries are the United States, Canada, and the United Kingdom. The most productive journals are the Journal of Medical Internet Research (JMIR), JMIR Serious Games, and the Games for Health Journal; the most influential journals are Patient Education and Counselling, Medical Education, and Quality of Life Research. The most productive authors are Riva, del Piccolo, and Schwebel; the most influential authors are Finset, del Piccolo, and Eide. The most frequently occurring keywords other than “virtual” and “reality” are “training,” “trial,” and “patients.” The most relevant research themes are communication, education, and novel treatments; the most recent research trends are fitness and exergames.
Conclusions:
The analysis shows that the field has left its infant state and its specialization is advancing, with a clear focus on patient usability.
Transitory starch plays a central role in the life cycle of plants. Many aspects of this important metabolism remain unknown; however, starch granules provide insight into this persistent metabolic process. Therefore, monitoring alterations in starch granules with high temporal resolution provides one significant avenue to improve understanding. Here, a previously established method that combines LCSM and safranin-O staining for in vivo imaging of transitory starch granules in leaves of Arabidopsis thaliana was employed to demonstrate, for the first time, the alterations in starch granule size and morphology that occur both throughout the day and during leaf aging. Several starch-related mutants were included, which revealed differences among the generated granules. In ptst2 and sex1-8, the starch granules in old leaves were much larger than those in young leaves; however, the typical flattened discoid morphology was maintained. In ss4 and dpe2/phs1/ss4, the morphology of starch granules in young leaves was altered, with a more rounded shape observed. With leaf development, the starch granules became spherical exclusively in dpe2/phs1/ss4. Thus, the presented data provide new insights to contribute to the understanding of starch granule morphogenesis.
Research on problem solving offers insights into how humans process task-related information and which strategies they use (Newell and Simon, 1972; Öllinger et al., 2014). Problem solving can be defined as the search for possible changes in one's mind (Kahneman, 2003). In a recent study, Adams et al. (2021) assessed whether the predominant problem solving strategy when making changes involves adding or subtracting elements. In order to do this, they used several examples of simple problems, such as editing text or making visual patterns symmetrical, either in naturalistic settings or on-line. The essence of the authors' findings is a strong preference to add rather than subtract elements across a diverse range of problems, including the stabilizing of artifacts, creating symmetrical patterns, or editing texts. More specifically, they succeeded in demonstrating that “participants were less likely to identify advantageous subtractive changes when the task did not (vs. did) cue them to consider subtraction, when they had only one opportunity (vs. several) to recognize the shortcomings of an additive search strategy or when they were under a higher (vs. lower) cognitive load” (Adams et al., 2021, p. 258).
Addition and subtraction are generally defined as de-contextualized mathematical operations using abstract symbols (Russell, 1903/1938). Nevertheless, understanding of both symbols and operations is informed by everyday activities, such as making or breaking objects (Lakoff and Núñez, 2000; Fischer and Shaki, 2018). The universal attribution of “addition bias” or “subtraction neglect” to problem solving activities is perhaps a convenient shorthand but it overlooks influential framing effects beyond those already acknowledged in the report and the accompanying commentary (Meyvis and Yoon, 2021).
Most importantly, while Adams et al.'s study addresses an important issue, their very method of verbally instructing participants, together with lack of control over several known biases, might render their findings less than conclusive. Below, we discuss our concerns that emerged from the identified biases, namely those regarding the instructions and the experimental materials. Moreover, we refer to research from mathematical cognition that provides new insights into Adams et al.'s findings.
Eastern Africa has been a prime target for scientific drilling because it is rich in key paleoanthropological sites as well as in paleolakes, containing valuable paleoclimatic information on evolutionary time scales. The Hominin Sites and Paleolakes Drilling Project (HSPDP) explores these paleolakes with the aim of reconstructing environmental conditions around critical episodes of hominin evolution. Identification of biological taxa based on their sedimentary ancient DNA (sedaDNA) traces can contribute to understand past ecological and climatological conditions of the living environment of our ancestors. However, sedaDNA recovery from tropical environments is challenging because high temperatures, UV irradiation, and desiccation result in highly degraded DNA. Consequently, most of the DNA fragments in tropical sediments are too short for PCR amplification. We analyzed sedaDNA in the upper 70 m of the composite sediment core of the HSPDP drill site at Chew Bahir for eukaryotic remnants. We first tested shotgun high throughput sequencing which leads to metagenomes dominated by bacterial DNA of the deep biosphere, while only a small fraction was derived from eukaryotic, and thus probably ancient, DNA. Subsequently, we performed cross-species hybridization capture of sedaDNA to enrich ancient DNA (aDNA) from eukaryotic remnants for paleoenvironmental analysis, using established barcoding genes (cox1 and rbcL for animals and plants, respectively) from 199 species that may have had relatives in the past biosphere at Chew Bahir. Metagenomes yielded after hybridization capture are richer in reads with similarity to cox1 and rbcL in comparison to metagenomes without prior hybridization capture. Taxonomic assignments of the reads from these hybridization capture metagenomes also yielded larger fractions of the eukaryotic domain. For reads assigned to cox1, inferred wet periods were associated with high inferred relative abundances of putative limnic organisms (gastropods, green algae), while inferred dry periods showed increased relative abundances for insects. These findings indicate that cross-species hybridization capture can be an effective approach to enhance the information content of sedaDNA in order to explore biosphere changes associated with past environmental conditions, enabling such analyses even under tropical conditions.
Background
Relatively little is known about protective factors and the emergence and maintenance of positive outcomes in the field of adolescents with chronic conditions. Therefore, the primary aim of the study is to acquire a deeper understanding of the dynamic process of resilience factors, coping strategies and psychosocial adjustment of adolescents living with chronic conditions.
Methods/design
We plan to consecutively recruit N = 450 adolescents (12–21 years) from three German patient registries for chronic conditions (type 1 diabetes, cystic fibrosis, or juvenile idiopathic arthritis). Based on screening for anxiety and depression, adolescents are assigned to two parallel groups – “inconspicuous” (PHQ-9 and GAD-7 < 7) vs. “conspicuous” (PHQ-9 or GAD-7 ≥ 7) – participating in a prospective online survey at baseline and 12-month follow-up. At two time points (T1, T2), we assess (1) intra- and interpersonal resiliency factors, (2) coping strategies, and (3) health-related quality of life, well-being, satisfaction with life, anxiety and depression. Using a cross-lagged panel design, we will examine the bidirectional longitudinal relations between resiliency factors and coping strategies, psychological adaptation, and psychosocial adjustment. To monitor Covid-19 pandemic effects, participants are also invited to take part in an intermediate online survey.
Discussion
The study will provide a deeper understanding of adaptive, potentially modifiable processes and will therefore help to develop novel, tailored interventions supporting a positive adaptation in youths with a chronic condition. These strategies should not only support those at risk but also promote the maintenance of a successful adaptation.
Trial registration
German Clinical Trials Register (DRKS), no. DRKS00025125. Registered on May 17, 2021.
Models are useful tools for understanding and predicting ecological patterns and processes. Under ongoing climate and biodiversity change, they can greatly facilitate decision-making in conservation and restoration and help designing adequate management strategies for an uncertain future. Here, we review the use of spatially explicit models for decision support and to identify key gaps in current modelling in conservation and restoration. Of 650 reviewed publications, 217 publications had a clear management application and were included in our quantitative analyses. Overall, modelling studies were biased towards static models (79%), towards the species and population level (80%) and towards conservation (rather than restoration) applications (71%). Correlative niche models were the most widely used model type. Dynamic models as well as the gene-to-individual level and the community-to-ecosystem level were underrepresented, and explicit cost optimisation approaches were only used in 10% of the studies. We present a new model typology for selecting models for animal conservation and restoration, characterising model types according to organisational levels, biological processes of interest and desired management applications. This typology will help to more closely link models to management goals. Additionally, future efforts need to overcome important challenges related to data integration, model integration and decision-making. We conclude with five key recommendations, suggesting that wider usage of spatially explicit models for decision support can be achieved by 1) developing a toolbox with multiple, easier-to-use methods, 2) improving calibration and validation of dynamic modelling approaches and 3) developing best-practise guidelines for applying these models. Further, more robust decision-making can be achieved by 4) combining multiple modelling approaches to assess uncertainty, and 5) placing models at the core of adaptive management. These efforts must be accompanied by long-term funding for modelling and monitoring, and improved communication between research and practise to ensure optimal conservation and restoration outcomes.
Children’s physical fitness development and related moderating effects of age and sex are well documented, especially boys’ and girls’ divergence during puberty. The situation might be different during prepuberty. As girls mature approximately two years earlier than boys, we tested a possible convergence of performance with five tests representing four components of physical fitness in a large sample of 108,295 eight-year old third-graders. Within this single prepubertal year of life and irrespective of the test, performance increased linearly with chronological age, and boys outperformed girls to a larger extent in tests requiring muscle mass for successful performance. Tests differed in the magnitude of age effects (gains), but there was no evidence for an interaction between age and sex. Moreover, “physical fitness” of schools correlated at r = 0.48 with their age effect which might imply that "fit schools” promote larger gains; expected secular trends from 2011 to 2019 were replicated.
Quantitative geomorphic research depends on accurate topographic data often collected via remote sensing. Lidar, and photogrammetric methods like structure-from-motion, provide the highest quality data for generating digital elevation models (DEMs). Unfortunately, these data are restricted to relatively small areas, and may be expensive or time-consuming to collect. Global and near-global DEMs with 1 arcsec (∼30 m) ground sampling from spaceborne radar and optical sensors offer an alternative gridded, continuous surface at the cost of resolution and accuracy. Accuracy is typically defined with respect to external datasets, often, but not always, in the form of point or profile measurements from sources like differential Global Navigation Satellite System (GNSS), spaceborne lidar (e.g., ICESat), and other geodetic measurements. Vertical point or profile accuracy metrics can miss the pixel-to-pixel variability (sometimes called DEM noise) that is unrelated to true topographic signal, but rather sensor-, orbital-, and/or processing-related artifacts. This is most concerning in selecting a DEM for geomorphic analysis, as this variability can affect derivatives of elevation (e.g., slope and curvature) and impact flow routing. We use (near) global DEMs at 1 arcsec resolution (SRTM, ASTER, ALOS, TanDEM-X, and the recently released Copernicus) and develop new internal accuracy metrics to assess inter-pixel variability without reference data. Our study area is in the arid, steep Central Andes, and is nearly vegetation-free, creating ideal conditions for remote sensing of the bare-earth surface. We use a novel hillshade-filtering approach to detrend long-wavelength topographic signals and accentuate short-wavelength variability. Fourier transformations of the spatial signal to the frequency domain allows us to quantify: 1) artifacts in the un-projected 1 arcsec DEMs at wavelengths greater than the Nyquist (twice the nominal resolution, so > 2 arcsec); and 2) the relative variance of adjacent pixels in DEMs resampled to 30-m resolution (UTM projected). We translate results into their impact on hillslope and channel slope calculations, and we highlight the quality of the five DEMs. We find that the Copernicus DEM, which is based on a carefully edited commercial version of the TanDEM-X, provides the highest quality landscape representation, and should become the preferred DEM for topographic analysis in areas without sufficient coverage of higher-quality local DEMs.
Computation of the instantaneous phase and amplitude via the Hilbert Transform is a powerful tool of data analysis. This approach finds many applications in various science and engineering branches but is not proper for causal estimation because it requires knowledge of the signal’s past and future. However, several problems require real-time estimation of phase and amplitude; an illustrative example is phase-locked or amplitude-dependent stimulation in neuroscience. In this paper, we discuss and compare three causal algorithms that do not rely on the Hilbert Transform but exploit well-known physical phenomena, the synchronization and the resonance. After testing the algorithms on a synthetic data set, we illustrate their performance computing phase and amplitude for the accelerometer tremor measurements and a Parkinsonian patient’s beta-band brain activity.
Semi-natural habitats (SNHs) are becoming increasingly scarce in modern agricultural landscapes. This may reduce natural ecosystem services such as pest control with its putatively positive effect on crop production. In agreement with other studies, we recently reported wheat yield reductions at field borders which were linked to the type of SNH and the distance to the border. In this experimental landscape-wide study, we asked whether these yield losses have a biotic origin while analyzing fungal seed and fungal leaf pathogens, herbivory of cereal leaf beetles, and weed cover as hypothesized mediators between SNHs and yield. We established experimental winter wheat plots of a single variety within conventionally managed wheat fields at fixed distances either to a hedgerow or to an in-field kettle hole. For each plot, we recorded the fungal infection rate on seeds, fungal infection and herbivory rates on leaves, and weed cover. Using several generalized linear mixed-effects models as well as a structural equation model, we tested the effects of SNHs at a field scale (SNH type and distance to SNH) and at a landscape scale (percentage and diversity of SNHs within a 1000-m radius). In the dry year of 2016, we detected one putative biotic culprit: Weed cover was negatively associated with yield values at a 1-m and 5-m distance from the field border with a SNH. None of the fungal and insect pests, however, significantly affected yield, neither solely nor depending on type of or distance to a SNH. However, the pest groups themselves responded differently to SNH at the field scale and at the landscape scale. Our findings highlight that crop losses at field borders may be caused by biotic culprits; however, their negative impact seems weak and is putatively reduced by conventional farming practices.
Background
The metabolic syndrome (MetS) is a risk cluster for a number of secondary diseases. The implementation of prevention programs requires early detection of individuals at risk. However, access to health care providers is limited in structurally weak regions. Brandenburg, a rural federal state in Germany, has an especially high MetS prevalence and disease burden. This study aims to validate and test the feasibility of a setup for mobile diagnostics of MetS and its secondary diseases, to evaluate the MetS prevalence and its association with moderating factors in Brandenburg and to identify new ways of early prevention, while establishing a “Mobile Brandenburg Cohort” to reveal new causes and risk factors for MetS.
Methods
In a pilot study, setups for mobile diagnostics of MetS and secondary diseases will be developed and validated. A van will be equipped as an examination room using point-of-care blood analyzers and by mobilizing standard methods. In study part A, these mobile diagnostic units will be placed at different locations in Brandenburg to locally recruit 5000 participants aged 40-70 years. They will be examined for MetS and advice on nutrition and physical activity will be provided. Questionnaires will be used to evaluate sociodemographics, stress perception, and physical activity. In study part B, participants with MetS, but without known secondary diseases, will receive a detailed mobile medical examination, including MetS diagnostics, medical history, clinical examinations, and instrumental diagnostics for internal, cardiovascular, musculoskeletal, and cognitive disorders. Participants will receive advice on nutrition and an exercise program will be demonstrated on site. People unable to participate in these mobile examinations will be interviewed by telephone. If necessary, participants will be referred to general practitioners for further diagnosis.
Discussion
The mobile diagnostics approach enables early detection of individuals at risk, and their targeted referral to local health care providers. Evaluation of the MetS prevalence, its relation to risk-increasing factors, and the “Mobile Brandenburg Cohort” create a unique database for further longitudinal studies on the implementation of home-based prevention programs to reduce mortality, especially in rural regions.
Trial registration
German Clinical Trials Register, DRKS00022764; registered 07 October 2020—retrospectively registered.
The olfactomotor system is especially investigated by examining the sniffing in reaction to olfactory stimuli. The motor output of respiratory-independent muscles was seldomly considered regarding possible influences of smells. The Adaptive Force (AF) characterizes the capability of the neuromuscular system to adapt to external forces in a holding manner and was suggested to be more vulnerable to possible interfering stimuli due to the underlying complex control processes. The aim of this pilot study was to measure the effects of olfactory inputs on the AF of the hip and elbow flexors, respectively. The AF of 10 subjects was examined manually by experienced testers while smelling at sniffing sticks with neutral, pleasant or disgusting odours. The reaction force and the limb position were recorded by a handheld device. The results show, inter alia, a significantly lower maximal isometric AF and a significantly higher AF at the onset of oscillations by perceiving disgusting odours compared to pleasant or neutral odours (p < 0.001). The adaptive holding capacity seems to reflect the functionality of the neuromuscular control, which can be impaired by disgusting olfactory inputs. An undisturbed functioning neuromuscular system appears to be characterized by a proper length tension control and by an earlier onset of mutual oscillations during an external force increase. This highlights the strong connection of olfaction and motor control also regarding respiratory-independent muscles.
Comprior
(2021)
Background
Reproducible benchmarking is important for assessing the effectiveness of novel feature selection approaches applied on gene expression data, especially for prior knowledge approaches that incorporate biological information from online knowledge bases. However, no full-fledged benchmarking system exists that is extensible, provides built-in feature selection approaches, and a comprehensive result assessment encompassing classification performance, robustness, and biological relevance. Moreover, the particular needs of prior knowledge feature selection approaches, i.e. uniform access to knowledge bases, are not addressed. As a consequence, prior knowledge approaches are not evaluated amongst each other, leaving open questions regarding their effectiveness.
Results
We present the Comprior benchmark tool, which facilitates the rapid development and effortless benchmarking of feature selection approaches, with a special focus on prior knowledge approaches. Comprior is extensible by custom approaches, offers built-in standard feature selection approaches, enables uniform access to multiple knowledge bases, and provides a customizable evaluation infrastructure to compare multiple feature selection approaches regarding their classification performance, robustness, runtime, and biological relevance.
Conclusion
Comprior allows reproducible benchmarking especially of prior knowledge approaches, which facilitates their applicability and for the first time enables a comprehensive assessment of their effectiveness
Objective
The Caribbean is an important global biodiversity hotspot. Adaptive radiations there lead to many speciation events within a limited period and hence are particularly prominent biodiversity generators. A prime example are freshwater fish of the genus Limia, endemic to the Greater Antilles. Within Hispaniola, nine species have been described from a single isolated site, Lake Miragoâne, pointing towards extraordinary sympatric speciation. This study examines the evolutionary history of the Limia species in Lake Miragoâne, relative to their congeners throughout the Caribbean.
Results
For 12 Limia species, we obtained almost complete sequences of the mitochondrial cytochrome b gene, a well-established marker for lower-level taxonomic relationships. We included sequences of six further Limia species from GenBank (total N = 18 species). Our phylogenies are in concordance with other published phylogenies of Limia. There is strong support that the species found in Lake Miragoâne in Haiti are monophyletic, confirming a recent local radiation. Within Lake Miragoâne, speciation is likely extremely recent, leading to incomplete lineage sorting in the mtDNA. Future studies using multiple unlinked genetic markers are needed to disentangle the relationships within the Lake Miragoâne clade.
The intensification of Northern Hemisphere glaciations at the end of the Pliocene epoch marks one of the most substantial climatic shifts of the Cenozoic. Despite global cooling, sea surface temperatures in the high latitude North Atlantic Ocean rose between 2.9–2.7 million years ago. Here we present sedimentary geochemical proxy data from the Gulf of Cadiz to reconstruct the variability of Mediterranean Outflow Water, an important heat source to the North Atlantic. We find evidence for enhanced production of Mediterranean Outflow from the mid-Pliocene to the late Pliocene which we infer could have driven a sub-surface heat channel into the high-latitude North Atlantic. We then use Earth System Models to constrain the impact of enhanced Mediterranean Outflow production on the northward heat transport in the North Atlantic. In accord with the proxy data, the numerical model results support the formation of a sub-surface channel that pumped heat from the subtropics into the high latitude North Atlantic. We further suggest that this mechanism could have delayed ice sheet growth at the end of the Pliocene.
Strong as a Hippo’s Heart: Biomechanical Hippo Signaling During Zebrafish Cardiac Development
(2021)
The heart is comprised of multiple tissues that contribute to its physiological functions. During development, the growth of myocardium and endocardium is coupled and morphogenetic processes within these separate tissue layers are integrated. Here, we discuss the roles of mechanosensitive Hippo signaling in growth and morphogenesis of the zebrafish heart. Hippo signaling is involved in defining numbers of cardiac progenitor cells derived from the secondary heart field, in restricting the growth of the epicardium, and in guiding trabeculation and outflow tract formation. Recent work also shows that myocardial chamber dimensions serve as a blueprint for Hippo signaling-dependent growth of the endocardium. Evidently, Hippo pathway components act at the crossroads of various signaling pathways involved in embryonic zebrafish heart development. Elucidating how biomechanical Hippo signaling guides heart morphogenesis has direct implications for our understanding of cardiac physiology and pathophysiology.
Implementing innovation laboratories to leverage intrapreneurship are an increasingly popular organizational practice. A typical feature in these creative environments are semi-autonomous teams in which multiple members collectively exert leadership influence, thereby challenging traditional command-and-control conceptions of leadership. An extensive body of research on the team-centric concept of shared leadership has recognized the potential for pluralized leadership structures in enhancing team effectiveness; however, little empirical work has been conducted in organizational contexts in which creativity is key. This study set out to explore antecedents of shared leadership and its influence on team creativity in an innovation lab. Building on extant shared leadership and innovation research, we propose antecedents customary to creative teamwork, that is, experimental culture, task reflexivity, and voice. Multisource data were collected from 104 team members and 49 evaluations of 29 coaches nested in 21 teams working in a prototypical innovation lab. We identify factors specific to creative teamwork that facilitate the emergence of shared leadership by providing room for experimentation, encouraging team members to speak up in the creative process, and cultivating a reflective application of entrepreneurial thinking. We provide specific exemplary activities for innovation lab teams to increase levels of shared leadership.
Objective
Insulin regulates mitochondrial function, thereby propagating an efficient metabolism. Conversely, diabetes and insulin resistance are linked to mitochondrial dysfunction with a decreased expression of the mitochondrial chaperone HSP60. The aim of this investigation was to determine the effect of a reduced HSP60 expression on the development of obesity and insulin resistance.
Methods
Control and heterozygous whole-body HSP60 knockout (Hsp60+/−) mice were fed a high-fat diet (HFD, 60% calories from fat) for 16 weeks and subjected to extensive metabolic phenotyping. To understand the effect of HSP60 on white adipose tissue, microarray analysis of gonadal WAT was performed, ex vivo experiments were performed, and a lentiviral knockdown of HSP60 in 3T3-L1 cells was conducted to gain detailed insights into the effect of reduced HSP60 levels on adipocyte homeostasis.
Results
Male Hsp60+/− mice exhibited lower body weight with lower fat mass. These mice exhibited improved insulin sensitivity compared to control, as assessed by Matsuda Index and HOMA-IR. Accordingly, insulin levels were significantly reduced in Hsp60+/− mice in a glucose tolerance test. However, Hsp60+/− mice exhibited an altered adipose tissue metabolism with elevated insulin-independent glucose uptake, adipocyte hyperplasia in the presence of mitochondrial dysfunction, altered autophagy, and local insulin resistance.
Conclusions
We discovered that the reduction of HSP60 in mice predominantly affects adipose tissue homeostasis, leading to beneficial alterations in body weight, body composition, and adipocyte morphology, albeit exhibiting local insulin resistance.
Previous studies have not considered the potential influence of maturity status on the relationship between mental imagery and change of direction (CoD) speed in youth soccer. Accordingly, this cross-sectional study examined the association between mental imagery and CoD performance in young elite soccer players of different maturity status. Forty young male soccer players, aged 10-17 years, were assigned into two groups according to their predicted age at peak height velocity (PHV) (Pre-PHV; n = 20 and Post-PHV; n = 20). Participants were evaluated on soccer-specific tests of CoD with (CoDBall-15m) and without (CoD-15m) the ball. Participants completed the movement imagery questionnaire (MIQ) with the three- dimensional structure, internal visual imagery (IVI), external visual imagery (EVI), as well as kinesthetic imagery (KI). The Post-PHV players achieved significantly better results than Pre-PHV in EVI (ES = 1.58, large; p < 0.001), CoD-15m (ES = 2.09, very large; p < 0.001) and CoDBall-15m (ES = 1.60, large; p < 0.001). Correlations were significantly different between maturity groups, where, for the pre-PHV group, a negative very large correlation was observed between CoDBall-15m and KI (r = –0.73, p = 0.001). For the post-PHV group, large negative correlations were observed between CoD-15m and IVI (r = –0.55, p = 0.011), EVI (r = –062, p = 0.003), and KI (r = –0.52, p = 0.020). A large negative correlation of CoDBall-15m with EVI (r = –0.55, p = 0.012) and very large correlation with KI (r = –0.79, p = 0.001) were also observed. This study provides evidence of the theoretical and practical use for the CoD tasks stimulus with imagery. We recommend that sport psychology specialists, coaches, and athletes integrated imagery for CoD tasks in pre-pubertal soccer players to further improve CoD related performance.
Sediment archives in the terrestrial and marine realm are regularly analyzed to infer changes in climate, tectonic, or anthropogenic boundary conditions of the past. However, contradictory observations have been made regarding whether short period events are faithfully preserved in stratigraphic archives; for instance, in marine sediments offshore large river systems. On the one hand, short period events are hypothesized to be non-detectable in the signature of terrestrially derived sediments due to buffering during sediment transport along large river systems. On the other hand, several studies have detected signals of short period events in marine records offshore large river systems. We propose that this apparent discrepancy is related to the lack of a differentiation between different types of signals and the lack of distinction between river response times and signal propagation times. In this review, we (1) expand the definition of the term ‘signal’ and group signals in sub-categories related to hydraulic grain size characteristics, (2) clarify the different types of ‘times’ and suggest a precise and consistent terminology for future use, and (3) compile and discuss factors influencing the times of signal transfer along sediment routing systems and how those times vary with hydraulic grain size characteristics. Unraveling different types of signals and distinctive time periods related to signal propagation addresses the discrepancies mentioned above and allows a more comprehensive exploration of event preservation in stratigraphy – a prerequisite for reliable environmental reconstructions from terrestrially derived sedimentary records.