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Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability.
Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability.
CovRadar
(2022)
The ongoing pandemic caused by SARS-CoV-2 emphasizes the importance of genomic surveillance to understand the evolution of the virus, to monitor the viral population, and plan epidemiological responses. Detailed analysis, easy visualization and intuitive filtering of the latest viral sequences are powerful for this purpose. We present CovRadar, a tool for genomic surveillance of the SARS-CoV-2 Spike protein. CovRadar consists of an analytical pipeline and a web application that enable the analysis and visualization of hundreds of thousand sequences. First, CovRadar extracts the regions of interest using local alignment, then builds a multiple sequence alignment, infers variants and consensus and finally presents the results in an interactive app, making accessing and reporting simple, flexible and fast.
-Christoph Markschies: Geleitwort
-Ulrich Päßler: Christian Gottfried Ehrenberg: Lebensbilder eines Naturforschers
-Mathias Grote: „Aus dem Kleinen bauen sich die Welten“ – Christian Gottfried Ehrenbergs ökologische Mikrobiologie avant la lettre
-Anne Greenwood MacKinney: Die Inszenierung naturforschender Gelehrsamkeit beim Sammeln: Christian Gottfried Ehrenbergs und Wilhelm Hemprichs nordafrikanische Forschungsreise (1820 – 1825)
-Ulrich Päßler: Reisen im Nahen Osten. Zeichnungen
-Ulrich Päßler: Christian Gottfried Ehrenberg und die Biogeographie: Die russisch-sibirische Reise mit Alexander von Humboldt (1829)
-Ulrich Päßler: Russisch-Sibirische Reise. Zeichnungen
-Wolf-Henning Kusber, Regine Jahn: Christian Gottfried Ehrenbergs Zeichnungen: Eine frühe wissenschaftliche Dokumentation mikroskopischer Organismen
-Ferdinand Damaschun: Christian Gottfried Ehrenberg und die Entwicklung der Mikroskop-Technik im 19. Jahrhundert
-Ulrich Päßler: Die Reise ins Kleinste der Natur. Zeichnungen
-Katrin Böhme: Das große Ganze: Christian Gottfried Ehrenberg und die Gesellschaft Naturforschender Freunde zu Berlin