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Institute
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Indoor mesocosm experiments were conducted to test for potential climate change effects on the spring succession of Baltic Sea plankton. Two different temperature (Delta 0 A degrees C and Delta 6 A degrees C) and three light scenarios (62, 57 and 49 % of the natural surface light intensity on sunny days), mimicking increasing cloudiness as predicted for warmer winters in the Baltic Sea region, were simulated. By combining experimental and modeling approaches, we were able to test for a potential dietary mismatch between phytoplankton and zooplankton. Two general predator-prey models, one representing the community as a tri-trophic food chain and one as a 5-guild food web were applied to test for the consequences of different temperature sensitivities of heterotrophic components of the plankton. During the experiments, we observed reduced time-lags between the peaks of phytoplankton and protozoan biomass in response to warming. Microzooplankton peak biomass was reached by 2.5 day A degrees C-1 earlier and occurred almost synchronously with biomass peaks of phytoplankton in the warm mesocosms (Delta 6 A degrees C). The peak magnitudes of microzooplankton biomass remained unaffected by temperature, and growth rates of microzooplankton were higher at Delta 6 A degrees C (mu(a dagger 0 A degrees C) = 0.12 day(-1) and mu(a dagger 6 A degrees C) = 0.25 day(-1)). Furthermore, warming induced a shift in microzooplankton phenology leading to a faster species turnover and a shorter window of microzooplankton occurrence. Moderate differences in the light levels had no significant effect on the time-lags between autotrophic and heterotrophic biomass and on the timing, biomass maxima and growth rate of microzooplankton biomass. Both models predicted reduced time-lags between the biomass peaks of phytoplankton and its predators (both microzooplankton and copepods) with warming. The reduction of time-lags increased with increasing Q(10) values of copepods and protozoans in the tritrophic food chain. Indirect trophic effects modified this pattern in the 5-guild food web. Our study shows that instead of a mismatch, warming might lead to a stronger match between protist grazers and their prey altering in turn the transfer of matter and energy toward higher trophic levels.
Label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization/denaturation by means of an array of individually addressable field-effect-based nanoplate silicon-on-insulator (SOI) capacitors modified with gold nanoparticles (Au-NP) is investigated. The proposed device detects charge changes on Au-NP/DNA hybrids induced by the hybridization or denaturation event. DNA hybridization was performed in a high ionic-strength solution to provide a high hybridization efficiency. On the other hand, to reduce the screening of the DNA charge by counter ions and to achieve a high sensitivity, the sensor signal induced by the hybridization and denaturation events was measured in a low ionic-strength solution. High sensor signals of about 120, 90, and 80 mV were registered after the DNA hybridization, denaturation, and re-hybridization events, respectively. Fluorescence microscopy has been applied as reference method to verify the DNA immobilization, hybridization, and denaturation processes. An electrostatic charge-plane model for potential changes at the gate surface of a nanoplate field-effect sensor induced by the DNA hybridization has been developed taking into account both the Debye length and the distance of the DNA charge from the gate surface.
The 2010 very high energy gamma-ray flare and 10 years ofmulti-wavelength oservations of M 87
(2012)
The giant radio galaxy M 87 with its proximity (16 Mpc), famous jet, and very massive black hole ((3-6) x 10(9) M-circle dot) provides a unique opportunity to investigate the origin of very high energy (VHE; E > 100 GeV) gamma-ray emission generated in relativistic outflows and the surroundings of supermassive black holes. M 87 has been established as a VHE gamma-ray emitter since 2006. The VHE gamma-ray emission displays strong variability on timescales as short as a day. In this paper, results from a joint VHE monitoring campaign on M 87 by the MAGIC and VERITAS instruments in 2010 are reported. During the campaign, a flare at VHE was detected triggering further observations at VHE (H.E.S.S.), X-rays (Chandra), and radio (43 GHz Very Long Baseline Array, VLBA). The excellent sampling of the VHE gamma-ray light curve enables one to derive a precise temporal characterization of the flare: the single, isolated flare is well described by a two-sided exponential function with significantly different flux rise and decay times of tau(rise)(d) = (1.69 +/- 0.30) days and tau(decay)(d) = (0.611 +/- 0.080) days, respectively. While the overall variability pattern of the 2010 flare appears somewhat different from that of previous VHE flares in 2005 and 2008, they share very similar timescales (similar to day), peak fluxes (Phi(>0.35 TeV) similar or equal to (1-3) x 10(-11) photons cm(-2) s(-1)), and VHE spectra. VLBA radio observations of 43 GHz of the inner jet regions indicate no enhanced flux in 2010 in contrast to observations in 2008, where an increase of the radio flux of the innermost core regions coincided with a VHE flare. On the other hand, Chandra X-ray observations taken similar to 3 days after the peak of the VHE gamma-ray emission reveal an enhanced flux from the core (flux increased by factor similar to 2; variability timescale <2 days). The long-term (2001-2010) multi-wavelength (MWL) light curve of M 87, spanning from radio to VHE and including data from Hubble Space Telescope, Liverpool Telescope, Very Large Array, and European VLBI Network, is used to further investigate the origin of the VHE gamma-ray emission. No unique, common MWL signature of the three VHE flares has been identified. In the outer kiloparsec jet region, in particular in HST-1, no enhanced MWL activity was detected in 2008 and 2010, disfavoring it as the origin of the VHE flares during these years. Shortly after two of the three flares (2008 and 2010), the X-ray core was observed to be at a higher flux level than its characteristic range (determined from more than 60 monitoring observations: 2002-2009). In 2005, the strong flux dominance of HST-1 could have suppressed the detection of such a feature. Published models for VHE gamma-ray emission from M 87 are reviewed in the light of the new data.
A growing literature has suggested that processing of visual information presented near the hands is facilitated. In this study, we investigated whether the near-hands superiority effect also occurs with the hands moving. In two experiments, participants performed a cyclical bimanual movement task requiring concurrent visual identification of briefly presented letters. For both the static and dynamic hand conditions, the results showed improved letter recognition performance with the hands closer to the stimuli. The finding that the encoding advantage for near-hand stimuli also occurred with the hands moving suggests that the effect is regulated in real time, in accordance with the concept of a bimodal neural system that dynamically updates hand position in external space.
In both eukaryotic and prokaryotic DNA sequences of 30-100 base-pairs rich in AT base-pairs have been identified at which the double helix preferentially unwinds. Such DNA unwinding elements are commonly associated with origins for DNA replication and transcription, and with chromosomal matrix attachment regions. Here we present a quantitative study of local DNA unwinding based on extensive single DNA plasmid imaging. We demonstrate that long-lived single-stranded denaturation bubbles exist in negatively supercoiled DNA, at the expense of partial twist release. Remarkably, we observe a linear relation between the degree of supercoiling and the bubble size, in excellent agreement with statistical modelling. Furthermore, we obtain the full distribution of bubble sizes and the opening probabilities at varying salt and temperature conditions. The results presented herein underline the important role of denaturation bubbles in negatively supercoiled DNA for biological processes such as transcription and replication initiation in vivo.
Structural changes at the intra- as well as intermicellar level were induced by the LCST-type collapse transition of poly(N-isopropyl acrylamide) in ABA triblock copolymer micelles in water. The distinct process kinetics was followed in situ and in real-time using time-resolved small-angle neutron scattering (SANS), while a micellar solution of a triblock copolymer, consisting of two short deuterated polystyrene endblocks and a long thermoresponsive poly(N-isopropyl acrylamide) middle block, was heated rapidly above its cloud point. A very fast collapse together with a multistep aggregation behavior is observed. The findings of the transition occurring at several size and time levels may have implications for the design and application of such thermoresponsive self-assembled systems.
Organic geochemical proxy data from surface sediment samples and a sediment core from Lake Donggi Cona were used to infer environmental changes on the northeastern Tibetan Plateau spanning the last 18.4 kyr. Long-chain n-alkanes dominate the aliphatic hydrocarbon fraction of the sediment extract from most surface sediment samples and the sediment core. Unsaturated mid-chain n-alkanes (nC(23:1) and nC(25:1)) have high abundances in some samples, especially in core samples from the late glacial and early Holocene. TOC contents, organic biomarker and non-pollen-palynomorph concentrations and results from organic petrologic analysis on selected samples suggest three major episodes in the history of Lake Donggi Cona. Before ca. 12.6 cal ka BP samples contain low amounts of organic matter due to cold and arid conditions during the late glacial. After 12.6 cal ka BP, relatively high contents of TOC and concentrations of Botryococcus fossils, as well as enhanced concentrations of mid-chain n-alkanes and n-alkenes suggest a higher primary and macrophyte productivity than at present This is supported by high contents of palynomorphs derived from higher plants and algae and was possibly triggered by a decrease of salinity and amelioration of climate during the early Holocene. Since 6.8 cal ka BP Lake Donggi Cona has been an oligotrophic freshwater lake. Proxy data suggest that variations in insolation drive ecological changes in the lake, with increased aquatic productivity during the early Holocene summer insolation maximum. Short-term drops of TOC contents or biomarker concentrations (at 9.9 cal ka BP, after 8.0 and between 3.5 and 1.7 cal ka BP) can possibly be related to relatively cool and dry episodes reported from other sites on the north-eastern Tibetan Plateau, which are hypothesized to occur in phase with Northern Hemisphere cooling events.
Afyon Zone, which was derived from the Anatolide-Tauride platform during closure of the Neo-Tethys, is made up of pre-Mesozoic basement and unconformably overlying Triassic-Early Tertiary cover series. The Afyon Zone contains widespread metavolcanic rocks, which are dominated by rhyolite, dacite, and trachyandesite. They form a distinct volcanic succession, which is separated from the underlying Silurian-Lower Carboniferous metacarbonates and meta-siliciclastics by a regional unconformity. Trachyandesitic metavolcanics are made up of massive lava flows, pyroclastics and epiclastics, less frequently, domes and dikes, which were developed on a deeply eroded subaerial landmass. U/Pb and Pb/Pb zircon geochronology yielded Lower Triassic (similar to 250 Ma) ages, which are interpreted as extrusion age of trachyandesitic volcanics. Based on the stratigraphic, geochronological, and geochemical data, we suggest that these Lower Triassic magmatic rocks represent an extensional tectonic setting on the northern active margin of the Gondwana, which led to the development of the northern branch of the Neo-Tethys.
Evaluation of groundwater dynamics and quality in the Najd aquifers located in the Sultanate of Oman
(2012)
The Najd, Oman, is located in one of the most arid environments in the world. The groundwater in this region is occurring in four different aquifers A to D of the Hadhramaut Group consisting mainly of different types of limestone and dolomite. The quality of the groundwater is dominated by the major ions sodium, calcium, magnesium, sulphate, and chloride, but the hydrochemical character is varying among the four aquifers. Mineralization within the separate aquifers increases along the groundwater flow direction from south to north-northeast up to high saline sodium-chloride water in aquifer D in the northeast area of the Najd. Environmental isotope analyses of hydrogen and oxygen were conducted to monitor the groundwater dynamics and to evaluate the recharge conditions of groundwater into the Najd aquifers. Results suggest an earlier recharge into these aquifers as well as ongoing recharge takes place in the region down to present day. Mixing of modern and submodern waters was detected by water isotopes in aquifer D in the mountain chain (Jabal) area and along the northern side of the mountain range. In addition, delta H-2 and delta O-18 variations suggest that aquifers A, B, and C are assumed to be connected by faults and fractures, and interaction between the aquifers may occur. Low tritium concentrations support the mixing assumption in the recharge area. The knowledge about the groundwater development is an important factor for the sustainable use of water resources in the Dhofar region.
A comprehensive numerical device simulation of the electrical and optical characteristics accompanied with experimental measurements of a new highly efficient system for polymer-based light-emitting diodes doped with phosphorescent dyes is presented. The system under investigation comprises an electron transporter attached to a polymer backbone blended with an electronically inert small molecule and an iridium-based green phosphorescent dye which serves as both emitter and hole transporter. The device simulation combines an electrical and an optical model. Based on the known highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) levels of all components as well as the measured electrical and optical characteristics of the devices, we model the emissive layer as an effective medium using the dye's HOMO as hole transport level and the polymer LUMO as electron transport level. By fine-tuning the injection barriers at the electron and hole-injecting contact, respectively, in simulated devices, unipolar device characteristics were fitted to the experimental data. Simulations using the so-obtained set of parameters yielded very good agreement to the measured currentvoltage, luminancevoltage characteristics, and the emission profile of entire bipolar light-emitting diodes, without additional fitting parameters. The simulation was used to gain insight into the physical processes and the mechanisms governing the efficiency of the organic light-emitting diode, including the position and extent of the recombination zone, carrier concentration profiles, and field distribution inside the device. The simulations show that the device is severely limited by hole injection, and that a reduction of the hole-injection barrier would improve the device efficiency by almost 50%.
Francesca Y. Albertini (1974-2011) compares Maimonides' idea of peace, as developed in MT Sefer shofetim (Book of Judges), with Kant's work on the notion of "eternal peace" (Zum ewigen Frieden). Both authors develop a historical vision pointed against the use of force and war in light of a framework not limited by historical time (messianic age, eternity). Despite all differences in method and historical context, the authors agree on the notion that universal ethics provides the basis of a determination of right grounded in the will. Maimonides' universal messianism as well as Kant's universal history emphasize the pivotal role and decisive responsibility of the human being in realizing, through reason, the reign of peace and prosperity on earth first envisioned by the biblical prophets. These utopias continue to challenge us, especially in this day and age.
A novel fluorinated copolymer (F-PCPDTBT) is introduced and shown to exhibit significantly higher power conversion efficiency in bulk heterojunction solar cells with PC70BM compared to the well-known low-band-gap polymer PCPDTBT. Fluorination lowers the polymer HOMO level, resulting in high open-circuit voltages well exceeding 0.7 V. Optical spectroscopy and morphological studies with energy-resolved transmission electron microscopy reveal that the fluorinated polymer aggregates more strongly in pristine and blended layers, with a smaller amount of additives needed to achieve optimum device performance. Time-delayed collection field and charge extraction by linearly increasing voltage are used to gain insight into the effect of fluorination on the field dependence of free charge-carrier generation and recombination. F-PCPDTBT is shown to exhibit a significantly weaker field dependence of free charge-carrier generation combined with an overall larger amount of free charges, meaning that geminate recombination is greatly reduced. Additionally, a 3-fold reduction in non-geminate recombination is measured compared to optimized PCPDTBT blends. As a consequence of reduced non-geminate recombination, the performance of optimized blends of fluorinated PCPDTBT with PC70BM is largely determined by the field dependence of free-carrier generation, and this field dependence is considerably weaker compared to that of blends comprising the non-fluorinated polymer. For these optimized blends, a short-circuit current of 14 mA/cm(2), an open-circuit voltage of 0.74 V, and a fill factor of 58% are achieved, giving a highest energy conversion efficiency of 6.16%. The superior device performance and the low band-gap render this new polymer highly promising for the construction of efficient polymer-based tandem solar cells.
We compare standard and inverted bulk heterojunction solar cells composed of PCPDTBT:PC70BM blends. Inverted devices comprising 100 nm thick active layers exhibited short circuit currents of 15 mA/cm(2), 10% larger than in corresponding standard devices. Modeling of the optical field distribution in the different device stacks proved that this enhancement originates from an increased absorption of incident light in the active layer. Internal quantum efficiencies (IQEs) were obtained from the direct comparison of experimentally derived and modeled currents for different layer thicknesses, yielding IQEs of similar to 70% for a layer thickness of 100 nm. Simulations predict a significant increase of the light harvesting efficiency upon increasing the layer thickness to 270 nm. However, a continuous deterioration of the photovoltaic properties with layer thickness was measured for both device architectures, attributed to incomplete charge extraction. On the other hand, our optical modeling suggests that inverted devices based on PCPDTBT should be able to deliver high power conversion efficiencies (PCEs) of more than 7% provided that recombination losses can be reduced.
We have applied time-delayed collection field (TDCF) and charge extraction by linearly increasing voltage (CELIV) to investigate the photogeneration, transport, and recombination of charge carriers in blends composed of PCPDTBT/PC70BM processed with and without the solvent additive diiodooctane. The results suggest that the solvent additive has severe impacts on the elementary processes involved in the photon to collected electron conversion in these blends. First, a pronounced field dependence of the free carrier generation is found for both blends, where the field dependence is stronger without the additive. Second, the fate of charge carriers in both blends can be described with a rather high bimolecular recombination coefficients, which increase with decreasing internal field. Third, the mobility is three to four times higher with the additive. Both blends show a negative field dependence of mobility, which we suggest to cause bias-dependent recombination coefficients.
Recent observations and modeling studies emphasize the crucial role of fracture mechanics for the stability of ice shelves and thereby the evolution of ice sheets. Here we introduce a macroscopic fracture-density field into a prognostic continuum ice-flow model and compute its evolution incorporating the initiation and growth of fractures as well as their advection with two-dimensional ice flow. To a first approximation, fracture growth is assumed to depend on the spreading rate only, while fracture initiation is defined in terms of principal stresses. The inferred fracture-density fields compare well with observed elongate surface structures. Since crevasses and other deep-reaching fracture structures have been shown to influence the overall ice-shelf dynamics, we propose the fracture-density field introduced here be used as a measure for ice softening and decoupling of the ice flow in fracture-weakened zones. This may yield more accurate and realistic velocity patterns in prognostic simulations. Additionally, the memory of past fracture events links the calving front to the upstream dynamics. Thus the fracture-density field proposed here may be employed in fracture-based calving parameterizations. The aim of this study is to introduce the field and investigate which of the observed surface structures can be reproduced by the simplest physically motivated fracture source terms.
This study addresses the interactions of coffee storage proteins with coffee-specific phenolic compounds. Protein profiles, of Coffea arabica and Coffea canephora (var robusta) were compared. Major Phenolic compounds were extracted and analyzed with appropriate methods. The polyphenol-protein interactions during protein extraction have been addressed by different analytical setups [reversed-phase high-performance liquid chromatography (RP-HPLC), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS), and Trolox equivalent antioxidant capacity (TEAC) assays], with focus directed toward identification of covalent adduct formation. The results indicate that C. arabica proteins are more susceptible to these interactions and the polyphenol oxidase activity seems to be a crucial factor for the formation of these addition products. A tentative allocation of the modification type and site in the protein has been attempted. Thus, the first available in silico modeling of modified coffee proteins is reported. The extent of these modifications may contribute to the structure and function of "coffee melanoidins" and are discussed in the context of coffee flavor formation.
We report on the discovery of high-energy (HE; E > 0.1 GeV) and very high energy (VHE; E > 100 GeV) gamma-ray emission from the high-frequency-peaked BL Lac object RBS 0413. VERITAS, a ground-based gamma-ray observatory, detected VHE. rays from RBS 0413 with a statistical significance of 5.5 standard deviations (sigma) and a gamma-ray flux of (1.5 +/- 0.6(stat) +/- 0.7(syst)) x 10(-8) photons m(-2) s(-1) (similar to 1% of the Crab Nebula flux) above 250 GeV. The observed spectrum can be described by a power law with a photon index of 3.18 +/- 0.68(stat) +/- 0.30(syst). Contemporaneous observations with the Large Area Telescope (LAT) on the Fermi Gamma-ray Space Telescope detected HE gamma rays from RBS 0413 with a (stat)istical significance of more than 9 sigma, a power-law photon index of 1.57 +/- 0.12(stat-0.12sys')(+0.11) and a gamma-ray flux between 300 MeV and 300 GeV of (1.64 +/- 0.43(stat-0.22sys)(+ 0.31)) x 10(-5) photons m(-2) s(-1). We present the results from Fermi-LAT and VERITAS, including a spectral energy distribution modeling of the gamma-ray, quasi-simultaneous X-ray (Swift-XRT), ultraviolet (Swift-UVOT), and R-band optical (MDM) data. We find that, if conditions close to equipartition are required, both the combined synchrotron self-Compton/external-Compton and the lepto-hadronic models are preferred over a pure synchrotron self-Compton model.
We report on VERITAS very high energy (VHE; E >= 100 GeV) observations of six blazars selected from the Fermi Large Area Telescope First Source Catalog (1FGL). The gamma-ray emission from 1FGL sources was extrapolated up to the VHE band, taking gamma-ray absorption by the extragalactic background light into account. This allowed the selection of six bright, hard-spectrum blazars that were good candidate TeV emitters. Spectroscopic redshift measurements were attempted with the Keck Telescope for the targets without Sloan Digital Sky Survey spectroscopic data. No VHE emission is detected during the observations of the six sources described here. Corresponding TeV upper limits are presented, along with contemporaneous Fermi observations and non-concurrent Swift UVOT and X-Ray Telescope data. The blazar broadband spectral energy distributions (SEDs) are assembled and modeled with a single-zone synchrotron self-Compton model. The SED built for each of the six blazars shows a synchrotron peak bordering between the intermediate-and high-spectrum-peak classifications, with four of the six resulting in particle-dominated emission regions.
Multiwavelength observations of the AGN 1ES 0414+009 with veritas, FERMI-LAT, SWIFT-XRT, AND MDM
(2012)
We present observations of the BL Lac object 1ES 0414+009 in the >200 GeV gamma-ray band by the VERITAS array of Cherenkov telescopes. 1ES 0414+009 was observed by VERITAS between 2008 January and 2011 February, resulting in 56.2 hr of good quality pointed observations. These observations resulted in a detection of 822 events from the source corresponding to a statistical significance of 6.4 standard deviations (6.4 sigma) above the background. The source flux, showing no evidence for variability, is measured as (5.2 +/- 1.1(stat) +/- 2.6(sys)) x 10(-12) photons cm(-2) s(-1) above 200 GeV, equivalent to approximately 2% of the Crab Nebula flux above this energy. The differential photon spectrum from 230 GeV to 850 GeV is well fit by a power law with a photon index of Gamma = 3.4 +/- 0.5(stat) +/- 0.3(sys) and a flux normalization of (1.6 +/- 0.3(stat) +/- 0.8(sys)) x 10(-11) photons cm(-2) s(-1) at 300 GeV. We also present multiwavelength results taken in the optical (MDM), x-ray (Swift-XRT), and GeV (Fermi-LAT) bands and use these results to construct a broadband spectral energy distribution (SED). Modeling of this SED indicates that homogenous one-zone leptonic scenarios are not adequate to describe emission from the system, with a lepto-hadronic model providing a better fit to the data.
The VERITAS array of Cherenkov telescopes has carried out a deep observational program on the nearby dwarf spheroidal galaxy Segue 1. We report on the results of nearly 48 hours of good quality selected data, taken between January 2010 and May 2011. No significant gamma-ray emission is detected at the nominal position of Segue 1, and upper limits on the integrated flux are derived. According to recent studies, Segue 1 is the most dark matter-dominated dwarf spheroidal galaxy currently known. We derive stringent bounds on various annihilating and decaying dark matter particle models. The upper limits on the velocity-weighted annihilation cross-section are <sigma upsilon >(95%) (CL) less than or similar to 10(-23) cm(3) s(-1), improving our limits from previous observations of dwarf spheroidal galaxies by at least a factor of 2 for dark matter particle masses m(chi) greater than or similar to 300 GeV. The lower limits on the decay lifetime are at the level of tau(95%) (CL) greater than or similar to 10(24) s. Finally, we address the interpretation of the cosmic ray lepton anomalies measured by ATIC and PAMELA in terms of dark matter annihilation, and show that the VERITAS observations of Segue 1 disfavor such a scenario.
We report on very high energy (E > 100 GeV) gamma-ray observations of V407 Cygni, a symbiotic binary that underwent a nova outburst producing 0.1-10 GeV gamma rays during 2010 March 10-26. Observations were made with the Very Energetic Radiation Imaging Telescope Array System during 2010 March 19-26 at relatively large zenith angles due to the position of V407 Cyg. An improved reconstruction technique for large zenith angle observations is presented and used to analyze the data. We do not detect V407 Cygni and place a differential upper limit on the flux at 1.6 TeV of 2.3 x 10(-12) erg cm(-2) s(-1) (at the 95% confidence level). When considered jointly with data from Fermi-LAT, this result places limits on the acceleration of very high energy particles in the nova.
We present the results of a joint observational campaign between the Green Bank radio telescope and the VERITAS gamma-ray telescope, which searched for a correlation between the emission of very-high-energy (VHE) gamma rays (E-gamma > 150 GeV) and giant radio pulses (GRPs) from the Crab pulsar at 8.9 GHz. A total of 15,366 GRPs were recorded during 11.6 hr of simultaneous observations, which were made across four nights in 2008 December and in 2009 November and December. We searched for an enhancement of the pulsed gamma-ray emission within time windows placed around the arrival time of the GRP events. In total, eight different time windows with durations ranging from 0.033 ms to 72 s were positioned at three different locations relative to the GRP to search for enhanced gamma-ray emission which lagged, led, or was concurrent with, the GRP event. Furthermore, we performed separate searches on main pulse GRPs and interpulse GRPs and on the most energetic GRPs in our data sample. No significant enhancement of pulsed VHE emission was found in any of the preformed searches. We set upper limits of 5-10 times the average VHE flux of the Crab pulsar on the flux simultaneous with interpulse GRPs on single-rotation-period timescales. On similar to 8 s timescales around interpulse GRPs, we set an upper limit of 2-3 times the average VHE flux. Within the framework of recent models for pulsed VHE emission from the Crab pulsar, the expected VHE-GRP emission correlations are below the derived limits.
VERITAS has been monitoring the very-high-energy (VHE; > 100 GeV) gamma-ray activity of the radio galaxy M87 since 2007. During 2008, flaring activity on a timescale of a few days was observed with a peak flux of (0.70 +/- 0.16) x 10(-11) cm(-2) s(-1) at energies above 350 GeV. In 2010 April, VERITAS detected a flare from M 87 with peak flux of (2.71 +/- 0.68) x 10(-11) cm(-2) s(-1) for E > 350 GeV. The source was observed for six consecutive nights during the flare, resulting in a total of 21 hr of good-quality data. The most rapid flux variation occurred on the trailing edge of the flare with an exponential flux decay time of 0.90(-0.15)(+0.22) days. The shortest detected exponential rise time is three times as long, at 2.87(+1.65)(-0.99) days. The quality of the data sample is such that spectral analysis can be performed for three periods: rising flux, peak flux, and falling flux. The spectra obtained are consistent with power-law forms. The spectral index at the peak of the flare is equal to 2.19 +/- 0.07. There is some indication that the spectrum is softer in the falling phase of the flare than the peak phase, with a confidence level corresponding to 3.6 standard deviations. We discuss the implications of these results for the acceleration and cooling rates of VHE electrons in M 87 and the constraints they provide on the physical size of the emitting region.
Fluoropolymer piezoelectrets with tubular channels resonance behavior controlled by channel geometry
(2012)
Ferro- or piezoelectrets are dielectric materials with two elastically very different macroscopic phases and electrically charged interfaces between them. One of the newer piezoelectret variants is a system of two fluoroethylenepropylene (FEP) films that are first laminated around a polytetrafluoroethylene (PTFE) template. Then, by removing the PTFE template, a two-layer FEP structure with open tubular channels is obtained. After electrical charging, the channels form easily deformable macroscopic electric dipoles whose changes under mechanical or electrical stress lead to significant direct or inverse piezoelectricity, respectively. Here, different PTFE templates are employed to generate channel geometries that vary in height or width. It is shown that the control of the channel geometry allows a direct adjustment of the resonance frequencies in the tubular-channel piezoelectrets. By combining several different channel widths in a single ferroelectret, it is possible to obtain multiple resonance peaks that may lead to a rather flat frequency-response region of the transducer material. A phenomenological relation between the resonance frequency and the geometrical parameters of a tubular channel is also presented. This relation may help to design piezoelectrets with a specific frequency response.
A template-based lamination technique for the manufacture of ferroelectrets from uniform electret films was recently reported. In the present work, this technique is used to prepare similar ferroelectret structures from low-density polyethylene (LDPE) films and from fluoro-ethylene-propylene (FEP) copolymer films. A comparative analysis of the pressure-, temperature-, and frequency-dependent piezoelectric properties has been performed on the two ferroelectret systems. It is observed that the FEP ferroelectrets exhibit better piezoelectric responses and are thermally more stable. The difference between the piezoelectric d(33) coefficients of the two ferroelectret systems is partially explained here by their different elastic moduli. The anti-resonance peaks of both structures have been investigated by means of dielectric resonance spectroscopy and electroacoustic sound-pressure measurements. A difference of more than 10 kHz is observed between the anti-resonance frequencies of the two ferroelectret systems.
The Garzn Complex of the Garzn Massif in SW Colombia is composed of the Vergel Granulite Unit (VG) and the Las Margaritas Migmatite Unit (LMM). Previous studies reveal peak temperature conditions for the VG of about 740 A degrees C. The present study considers the remarkable exsolution phenomena in feldspars and pyroxenes and titanium-in-quartz thermometry. Recalculated ternary feldspar compositions indicate temperatures around 900-1,000 A degrees C just at or above the ultra-high temperature-metamorphism (UHTM) boundary of granulites. The calculated temperatures range of exsolved ortho- and clinopyroxenes also supports the existence of an UHTM event. In addition, titanium-in-quartz thermometry points towards ultra-high temperatures. It is the first known UHTM crustal segment in the northern part of South America. Although a mean geothermal gradient of ca 38 A degrees C km(-1) could imply additional heat supply in the lower crust controlling this extreme of peak metamorphism, an alternative model is suggested. The formation of the Vergel Granulite Unit is supposed to be formed in a continental back-arc environment with a thinned and weakened crust behind a magmatic arc (Guapotn-Mancagua Gneiss) followed by collision. In contrast, rocks of the adjacent Las Margaritas Migmatite Unit display "normal" granulite facies temperatures and are formed in a colder lower crust outside the arc, preserved by the Guapotn-Mancagu Gneiss. Back-arc formation was followed by inversion and thickening of the basin. The three units that form the modern-day Garzn Massif, were juxtaposed upon each other during collision (at ca. 1,000 Ma) and exhumation. The collision leading to the deformation of the studied area is part of the Grenville orogeny leading to the amalgamation of Rodinia.
Background: As the prevalence of diabetes rises, its complications such as diabetic nephropathy affect an increaseing number of patients. Consequently, the need for biomarkers in rodent models which reflect the stage and course of diabetic nephropathy is high. This article focuses on Heart-type fatty acid binding protein (H-FABP), osteopontin (OPN), nephrin, and Neutrophil gelatinase-associated lipocalin (NGAL) in urine, and kidney injury molecule (KIM)-1, clusterin, and tissue inhibitior of metalloproteinases (TIMP) 1 in plasma in uni-nephrectomized rats with streptocotozin-induced type 1 diabetes mellitus, a common animal model to explore renal impairment in the setting of diabetes mellitus.
Methods: 23 male Wistar rats were uni-nephrectomized and subsequently divided into two study groups. The diabetic group received streptozotocin (STZ) via tail-vein injection, the non-diabetic group received citrate buffer without STZ. Subsequently, blood glucose, body weight, and blood pressure were checked regularly. After 18 weeks, animals were placed in metabolic cages, blood and urine obtained and subsequently organs were harvested after sacrifice.
Results: Blood glucose levels were highly increased in diabetic animals throughout the experiment, whereas systolic blood pressure did not differ between the study groups. At study end, classical biomarkers such as urinary albumin and protein and plasma cystatin c were only slightly but not significantly different between groups indicating a very early disease state. In contrast, urinary excretion of H-FABP, OPN, nephrin, and NGAL were highly increased in diabetic animals with a highly significant p-value (p<0.01 each) compared to non-diabetic animals. In plasma, differences were found for calbindin, KIM-1, clusterin, TIMP-1, and OPN. These findings were confirmed by means of the area under the receiver operating characteristic curve (ROC-AUC) analysis.
Conclusions: In summary, our study revealed elevated levels of new plasma and urinary biomarkers (urinary osteopontin, urinary nephrin, urinary NGAL, urinary H-FABP, plasma KIM-1, plasma TIMP-1) in uni-nephrectomized diabetic rats, an established rat model of diabetic nephropathy. These biomarkers appeared even before the classical biomarkers of diabetic nephropathy such as albuminuria and urinary protein excretion. The new biomarkers might offer advantage to urinary albumin and plasma cystatin c with respect to early detection.
Background: The need for an improved treatment for diabetic nephropathy is greatest in patients who do not adequately respond to angiotensin II receptor blockers (ARBs). This study investigated the effect of the novel dipeptidyl peptidase-4 inhibitor linagliptin alone and in combination with the ARB telmisartan on the progression of diabetic nephropathy in diabetic endothelial nitric oxide synthase (eNOS) knockout mice. Methods: Sixty male eNOS knockout C57BL/6J mice were divided into four groups after receiving intraperitoneal high-dose streptozotocin: telmisartan (1 mg/kg), linagliptin (3 mg/kg), linagliptin + telmisartan (3 mg/kg + 1 mg/kg) and vehicle. Fourteen mice were used as non-diabetic controls. Results: After 12 weeks, urine and blood were obtained and blood pressure measured. Glucose concentrations were increased and similar in all diabetic groups. Telmisartan alone reduced systolic blood pressure by 5.9 mmHg versus diabetic controls (111.2 +/- 2.3 mmHg vs 117.1 +/- 2.2 mmHg; mean +/- SEM; P = 0.071). Combined treatment significantly reduced albuminuria compared with diabetic controls (71.7 +/- 15.3 mu g/24 h vs 170.8 +/- 34.2 mu g/24 h; P = 0.017), whereas the effects of single treatment with either telmisartan (97.8 +/- 26.4 mu g/24 h) or linagliptin (120.8 +/- 37.7 mu g/24 h) were not statistically significant. DPP-4 inhibition, alone and in combination, led to significantly lower plasma osteopontin levels compared with telmisartan alone. Histological analysis revealed reduced glomerulosclerosis after Linagliptin alone and in combination with telmisartan in comparison to non treated diabetic animals (p < 0.01 and p < 0.05). Kidney malonaldehyde immune-reactivity, a marker of oxidative stress, was significantly lower in animals treated with linagliptin. Conclusions: DPP-4 inhibition on top of ARB treatment significantly reduced urinary albumin excretion and oxidative stress in diabetic eNOS knockout mice. Linagliptin on top of an angiotensin II receptor blocker may offer a new therapeutic approach for patients with diabetic nephropathy.
Each simulation algorithm, including Truncated Gaussian Simulation, Sequential Indicator Simulation and Indicator Kriging is characterized by different operating modes, which variably influence the facies proportion, distribution and association of digital outcrop models, as shown in clastic sediments. A detailed study of carbonate heterogeneity is then crucial to understanding these differences and providing rules for carbonate modelling. Through a continuous exposure of Bajocian carbonate strata, a study window (320 m long, 190 m wide and 30 m thick) was investigated and metre-scale lithofacies heterogeneity was captured and modelled using closely-spaced sections. Ten lithofacies, deposited in a shallow-water carbonate-dominated ramp, were recognized and their dimensions and associations were documented. Field data, including height sections, were georeferenced and input into the model. Four models were built in the present study. Model A used all sections and Truncated Gaussian Simulation during the stochastic simulation. For the three other models, Model B was generated using Truncated Gaussian Simulation as for Model A, Model C was generated using Sequential Indicator Simulation and Model D was generated using Indicator Kriging. These three additional models were built by removing two out of eight sections from data input. The removal of sections allows direct insights on geological uncertainties at inter-well spacings by comparing modelled and described sections. Other quantitative and qualitative comparisons were carried out between models to understand the advantages/disadvantages of each algorithm. Model A is used as the base case. Indicator Kriging (Model D) simplifies the facies distribution by assigning continuous geological bodies of the most abundant lithofacies to each zone. Sequential Indicator Simulation (Model C) is confident to conserve facies proportion when geological heterogeneity is complex. The use of trend with Truncated Gaussian Simulation is a powerful tool for modelling well-defined spatial facies relationships. However, in shallow-water carbonate, facies can coexist and their association can change through time and space. The present study shows that the scale of modelling (depositional environment or lithofacies) involves specific simulation constraints on shallow-water carbonate modelling methods.
The chemotaxis of eukaryotic cells depends both on the average concentration of the chemoattractant and on the steepness of its gradient. For the social amoeba Dictyostelium discoideum, we test quantitatively the prediction by Ueda and Shibata [Biophys. J. 93, 11 (2007)] that the efficacy of chemotaxis depends on a single control parameter only, namely, the signal-to-noise ratio (SNR), determined by the stochastic fluctuations of (i) the binding of the chemoattractant molecule to the transmembrane receptor and (ii) the intracellular activation of the effector of the signaling cascade. For SNR less than or similar to 1, the theory captures the experimental findings well, while for larger SNR noise sources further downstream in the signaling pathway need to be taken into account.
Chemotaxis, the directed motion of a cell toward a chemical source, plays a key role in many essential biological processes. Here, we derive a statistical model that quantitatively describes the chemotactic motion of eukaryotic cells in a chemical gradient. Our model is based on observations of the chemotactic motion of the social ameba Dictyostelium discoideum, a model organism for eukaryotic chemotaxis. A large number of cell trajectories in stationary, linear chemoattractant gradients is measured, using microfluidic tools in combination with automated cell tracking. We describe the directional motion as the interplay between deterministic and stochastic contributions based on a Langevin equation. The functional form of this equation is directly extracted from experimental data by angle-resolved conditional averages. It contains quadratic deterministic damping and multiplicative noise. In the presence of an external gradient, the deterministic part shows a clear angular dependence that takes the form of a force pointing in gradient direction. With increasing gradient steepness, this force passes through a maximum that coincides with maxima in both speed and directionality of the cells. The stochastic part, on the other hand, does not depend on the orientation of the directional cue and remains independent of the gradient magnitude. Numerical simulations of our probabilistic model yield quantitative agreement with the experimental distribution functions. Thus our model captures well the dynamics of chemotactic cells and can serve to quantify differences and similarities of different chemotactic eukaryotes. Finally, on the basis of our model, we can characterize the heterogeneity within a population of chemotactic cells.
Understanding the dynamics of sediment fluxes is a key issue to constrain modern erosion rates in mountain belts and determine the still debated level of control exerted by precipitation, topography and tectonics. The well defined monsoon seasonality in the Himalayas, together with active tectonics and strong relief provide an ideal environment to assess these possible interactions. For this purpose, we present a new compilation of daily suspended sediment data for 12 stations of the major rivers of the Nepal Himalayas. We analyze the relationships of sediment transport with daily river discharge and precipitation data as well as with morphometric parameters. We show that suspended sediment concentrations vary systematically through the seasons and asynchronously to river discharge displaying a hysteresis effect. This clockwise hysteresis effect disappears when suspended sediment fluxes are directly compared with direct storm discharge. Therefore we attribute the hysteresis effect to groundwater dilution rather than a sediment supply limitation. We infer a rating model to calculate erosion rates directly from long river discharge chronicles. We show that, when normalized by drainage area and mean sediment flux, all rivers exhibit the same trend. This similarity implies that all river basins have the same erosion behavior, independent of location, size and catchment characteristics. Erosion rates calculated from suspended sediment fluxes range between 0.1 and 2.8 mm/yr. The erosion rates of the three main basins of Nepal are in the range 0.9-1.5 mm/yr. Erosion rates in the Higher Himalayas are relatively low ( <0.5 mm/yr, except for Kali Gandaki), while in the Lesser Himalayas they range from 0.2 to 2 mm/yr. We propose that material transport in the rivers depends on hillslope sediment supply, which is, in turn, controlled by those rainfalls producing direct runoff. In other words, the rivers in the Nepal Himalayas are supply-limited and the hillsopes as a contributing source are transport-limited. We also show that erosion processes are not as much controlled by infrequently occurring extreme precipitation events, than by moderate ones with a high recurrence interval.
To contribute to a further insight into heterosis we applied an integrative analysis to a systems biological network approach and a quantitative genetics analysis towards biomass heterosis in early Arabidopsis thaliana development. The study was performed on the parental accessions C24 and Col-0 and the reciprocal crosses. In an over-representation analysis it was tested if the overlap between the resulting gene lists of the two approaches is significantly larger than expected by chance. Top ranked genes in the results list of the systems biological analysis were significantly over-represented in the heterotic QTL candidate regions for either hybrid as well as regarding mid-parent and best-parent heterosis. This suggests that not only a few but rather several genes that influence biomass heterosis are located within each heterotic QTL region. Furthermore, the overlapping resulting genes of the two integrated approaches were particularly enriched in biomass related pathways. A chromosome-wise over-representation analysis gave rise to the hypothesis that chromosomes number 2 and 4 probably carry a majority of the genes involved in biomass heterosis in the early development of Arabidopsis thaliana.
Bacteriophages use specific tail proteins to recognize host cells. It is still not understood to molecular detail how the signal is transmitted over the tail to initiate infection. We have analysed in vitro DNA ejection in long-tailed siphovirus 9NA and short-tailed podovirus P22 upon incubation with Salmonella typhimurium lipopolysaccharide (LPS). We showed for the first time that LPS alone was sufficient to elicit DNA release from a siphovirus in vitro. Crystal structure analysis revealed that both phages use similar tailspike proteins for LPS recognition. Tailspike proteins hydrolyse LPS O antigen to position the phage on the cell surface. Thus we were able to compare in vitro DNA ejection processes from two phages with different morphologies with the same receptor under identical experimental conditions. Siphovirus 9NA ejected its DNA about 30 times faster than podovirus P22. DNA ejection is under control of the conformational opening of the particle and has a similar activation barrier in 9NA and P22. Our data suggest that tail morphology influences the efficiencies of particle opening given an identical initial receptor interaction event.
We have undertaken structural, geomorphological, and morphometric analyses to investigate the role of tectonism and climate in the landscape evolution in the upper Spiti valley, NW Himalayas. Geomorphometric analyses coupled with field investigations reveal active tectonic deformation in the Spiti region. The calculated geomorphic indices (steepness, concavity and Hack) demonstrate uplift/subsidence along the Kaurik-Chango fault, whereas transverse topographic index (T-index) reveals basin tilting associated with active faulting near Hansa and Lingti valley. Investigation of well-dated Mane palaeolake sediments also provides evidence of regional tectonic instability. Four episodes (ca. 7.8, 7.4, 6.5 and 6.1 cal ka) of neotectonic activity have been identified during the period of the lake's existence. We have also compiled data on the regional climate variability and compared it with the age of the Mane palaeo-landslide. Our results indicate that the landslide occurred towards the end of the early Holocene intensified monsoon phase and is located near an active fault. Our data on regional tectonic instability and the coincidences of modern and palaeo-landslides with zones of active deformation suggest that tectonism is an important factor governing landscape stability in the Spiti region.
We measured memory span for assembly instructions involving objects with handles oriented to the left or right side. Right-handed participants remembered more instructions when objects' handles were spatially congruent with the hand used in forthcoming assembly actions. No such affordance-based memory benefit was found for left-handed participants. These results are discussed in terms of motor simulation as an embodied rehearsal mechanism.
The tricarboxylic acid (TCA) cycle is a crucial component of respiratory metabolism in both photosynthetic and heterotrophic plant organs. All of the major genes of the tomato TCA cycle have been cloned recently, allowing the generation of a suite of transgenic plants in which the majority of the enzymes in the pathway are progressively decreased. Investigations of these plants have provided an almost complete view of the distribution of control in this important pathway. Our studies suggest that citrate synthase, aconitase, isocitrate dehydrogenase, succinyl CoA ligase, succinate dehydrogenase, fumarase and malate dehydrogenase have control coefficients flux for respiration of -0.4, 0.964, -0.123, 0.0008, 0.289, 0.601 and 1.76, respectively; while 2-oxoglutarate dehydrogenase is estimated to have a control coefficient of 0.786 in potato tubers. These results thus indicate that the control of this pathway is distributed among malate dehydrogenase, aconitase, fumarase, succinate dehydrogenase and 2-oxoglutarate dehydrogenase. The unusual distribution of control estimated here is consistent with specific non-cyclic flux mode and cytosolic bypasses that operate in illuminated leaves. These observations are discussed in the context of known regulatory properties of the enzymes and some illustrative examples of how the pathway responds to environmental change are given.
Observations of radio halos and relics in galaxy clusters indicate efficient electron acceleration. Protons should likewise be accelerated and, on account of weak energy losses, can accumulate, suggesting that clusters may also be sources of very high energy (VHE; E > 100 GeV) gamma-ray emission. We report here on VHE gamma-ray observations of the Coma galaxy cluster with the VERITAS array of imaging Cerenkov telescopes, with complementing Fermi Large Area Telescope observations at GeV energies. No significant gamma-ray emission from the Coma Cluster was detected. Integral flux upper limits at the 99% confidence level were measured to be on the order of (2-5) x 10(-8) photonsm(-2) s(-1) (VERITAS, >220 GeV) and similar to 2 x 10(-6) photonsm(-2) s(-1) (Fermi, 1-3GeV), respectively. We use the gamma-ray upper limits to constrain cosmic rays (CRs) and magnetic fields in Coma. Using an analytical approach, the CR-to-thermal pressure ratio is constrained to be < 16% from VERITAS data and <1.7% from Fermi data (averaged within the virial radius). These upper limits are starting to constrain the CR physics in self-consistent cosmological cluster simulations and cap the maximum CR acceleration efficiency at structure formation shocks to be <50%. Alternatively, this may argue for non-negligible CR transport processes such as CR streaming and diffusion into the outer cluster regions. Assuming that the radio-emitting electrons of the Coma halo result from hadronic CR interactions, the observations imply a lower limit on the central magnetic field in Coma of similar to(2-5.5) mu G, depending on the radial magnetic field profile and on the gamma-ray spectral index. Since these values are below those inferred by Faraday rotation measurements in Coma (for most of the parameter space), this renders the hadronic model a very plausible explanation of the Coma radio halo. Finally, since galaxy clusters are dark matter (DM) dominated, the VERITAS upper limits have been used to place constraints on the thermally averaged product of the total self-annihilation cross section and the relative velocity of the DM particles, <sigma nu >.
The fluorescence response of a set of cyclam-triazole-dye ligands is controlled by the appended dye, but simple reversal of the triazole topology affords a novel probe for Zn2+ with a longer fluorescence lifetime and higher fluorescence quantum yield upon Zn2+ binding (<tau t > = 2.0 ns, Phi(f) = 0.76).
Companies have to adhere to compliance requirements. The compliance analysis of business operations is typically a joint effort of business experts and compliance experts. Those experts need to create a common understanding of business processes to effectively conduct compliance management. In this paper, we present a technique that aims at supporting this process. We argue that process templates generated out of compliance requirements provide a basis for negotiation among business and compliance experts. We introduce a semi-automated and iterative approach to the synthesis of such process templates from compliance requirements expressed in Linear Temporal Logic (LTL). We show how generic constraints related to business process execution are incorporated and present criteria that point at underspecification. Further, we outline how such underspecification may be resolved to iteratively build up a complete specification. For the synthesis, we leverage existing work on process mining and process restructuring. However, our approach is not limited to the control-flow perspective, but also considers direct and indirect data-flow dependencies. Finally, we elaborate on the application of the derived process templates and present an implementation of our approach. (C) 2012 Elsevier Ltd. All rights reserved.
A series of new heteroleptic MN2S2 transition metal complexes with M = Cu2+ for EPR measurements and as diamagnetic hosts Ni2+, Zn2+, and Pd2+ were synthesized and characterized. The ligands are N2 = 4, 4'-bis(tert-butyl)-2, 2'-bipyridine (tBu2bpy) and S2 =1, 2-dithiooxalate, (dto), 1, 2-dithiosquarate, (dtsq), maleonitrile-1, 2-dithiolate, or 1, 2-dicyanoethene-1, 2-dithiolate, (mnt). The CuII complexes were studied by EPR in solution and as powders, diamagnetically diluted in the isostructural planar [NiII(tBu2bpy)(S2)] or[PdII(tBu2bpy)(S2)] as well as in tetrahedrally coordinated[ZnII(tBu2bpy)(S2)] host structures to put steric stress on the coordination geometry of the central CuN2S2 unit. The spin density contributions for different geometries calculated from experimental parameters are compared with the electronic situation in the frontier orbital, namely in the semi-occupied molecular orbital (SOMO) of the copper complex, derived from quantum chemical calculations on different levels (EHT and DFT). One of the hosts, [NiII(tBu2bpy)(mnt)], is characterized by X-ray structure analysis to prove the coordination geometry. The complex crystallizes in a square-planar coordination mode in the monoclinic space group P21/a with Z = 4 and the unit cell parameters a = 10.4508(10) angstrom, b = 18.266(2) angstrom, c = 12.6566(12) angstrom, beta = 112.095(7)degrees. Oxidation and reductions potentials of one of the host complexes, [Ni(tBu2bpy)(mnt)], were obtained by cyclovoltammetric measurements.
This study examined the effects of competing speech on auditory semantic comprehension using a dichotic sentence-word priming paradigm. Lexical decision performance for target words presented in spoken sentences was compared in strongly and weakly biasing semantic contexts. Targets were either congruent or incongruent with the sentential bias. Sentences were presented to one auditory channel (right or left), either in isolation or with competing speech produced by a single talker of the same gender presented simultaneously. The competing speech signal was either presented in the same auditory channel as the sentence context, or in a different auditory channel, and was either meaningful (played forward) or unintelligible (time-reversed).
Biasing contexts presented in isolation facilitated responses to congruent targets and inhibited responses to incongruent targets, relative to a neutral baseline. Facilitation priming was reduced or eliminated by competing speech presented in the same auditory channel, supporting previous findings that semantic activation is highly sensitive to the intelligibility of the context signal. Competing speech presented in a different auditory channel affected facilitation priming differentially depending upon ear of presentation, suggesting hemispheric differences in the processing of the attended and competing signals. Results were consistent with previous claims of a right ear advantage for meaningful speech, as well as with visual word recognition findings implicating the left hemisphere in the generation of semantic predictions and the right hemisphere in the integration of newly encountered words into the sentence-level meaning. Unlike facilitation priming, inhibition was relatively robust to the energetic and informational masking effects of competing speech and was not influenced by the strength of the contextual bias or the meaningfulness of the competing signal, supporting a two-process model of sentence priming in which inhibition reflects later-stage, expectancy-driven strategic processes that may benefit from perceptual reanalysis after initial semantic activation.
We present a toy-model for an ensemble of adhering mesoscopic constituents in order to estimate the effect of the granular temperature on the sizes of embedded aggregates. The major goal is to illustrate the relation between the mean aggregate size and the granular temperature in dense planetary rings. For sake of simplicity we describe the collective behavior of the ensemble by means of equilibrium statistical mechanics, motivated by the stationary temperature established by the balance between a Kepler-shear driven viscous heating and inelastic cooling in these cosmic granular disks. The ensemble consists of N' equal constituents which can form cluster(s) or move like a gas-or both phases may coexist-depending on the (granular) temperature of the system. We assume the binding energy levels of a cluster E-c = -N-c gamma a to be determined by a certain contact number N-c, given by the configuration of N constituents of the aggregate (energy per contact: -gamma a). By applying canonical and grand-canonical ensembles, we show that the granular temperature T of a gas of constituents (their mean kinetic energy) controls the size distribution of the aggregates. They are the smaller the higher the granular temperature T is. A mere gas of single constituents is sustained for T >> gamma a. In the case of large clusters (low temperatures T << gamma a) the size distribution becomes a Poissonian.
Glycolate oxidase (GO) catalyses the oxidation of glycolate to glyoxylate, thereby consuming O-2 and producing H2O2. In this work, Arabidopsis thaliana plants expressing GO in the chloroplasts (GO plants) were used to assess the expressional behavior of reactive oxygen species (ROS)-responsive genes and transcription factors (TFs) after metabolic induction of H2O2 formation in chloroplasts. In this organelle, GO uses the glycolate derived from the oxygenase activity of RubisCO. Here, to identify genes responding to an abrupt production of H2O2 in chloroplasts we used quantitative real-time PCR (qRT-PCR) to test the expression of 187 ROS-responsive genes and 1880 TFs after transferring GO and wild-type (WT) plants grown at high CO2 levels to ambient CO2 concentration. Our data revealed coordinated expression changes of genes of specific functional networks 0.5 h after metabolic induction of H2O2 production in GO plants, including the induction of indole glucosinolate and camalexin biosynthesis genes. Comparative analysis using available microarray data suggests that signals for the induction of these genes through H2O2 may originate in the chloroplast. The TF profiling indicated an up-regulation in GO plants of a group of genes involved in the regulation of proanthocyanidin and anthocyanin biosynthesis. Moreover, the upregulation of expression of IF and IF interacting proteins affecting development (e.g., cell division, stem branching, flowering time, flower development) would impact growth and reproductive capacity, resulting in altered development under conditions that promote the formation of H2O2.
The minima on the potential energy surface of 1,2-bis(o-carboxyphenoxy)ethane (CPE) molecule in its electronic ground state were searched by a molecular dynamics simulation performed with MM2 force field. For each of the found minimum-energy conformers, the corresponding equilibrium geometry, charge distribution, HOMO-LUMO energy gap, force field, vibrational normal modes and associated IR and Raman spectral data were determined by means of the density functional theory (DFT) based electronic structure calculations carried out by using B3LYP method and various Pople-style basis sets. The obtained theoretical data confirmed the significant effects of the intra- and inter-molecular hydrogen bonding interactions on the conformational structure, force field, and group vibrations of the molecule. The same data have also revealed that two of the determined stable conformers, both of which exhibit pseudo-crown structure, are considerably more favorable in energy to the others and accordingly provide the major contribution to the experimental spectra of CPE. In the light of the improved vibrational spectral data obtained within the "SQM FF" methodology and "Dual Scale Factors" approach for the monomer and dimer forms of these two conformers, a reliable assignment of the fundamental bands observed in the experimental room-temperature IR and Raman spectra of the molecule was given, and the sensitivities of its group vibrations to conformation, substitution and dimerization were discussed.
Sedimentological, provenance, and detrital thermochronological results for basin fill at the modern deformation front of the northern Andes (6 degrees N latitude) provide a long-term, Eocene to Pliocene record of foreland-basin sedimentation along the Eastern Cordillera !Janos basin boundary in Colombia. Lithofacies assemblages and paleocurrent orientations in the upward-coarsening, 5-km-thick succession of the Nunchia syncline reveal a systematic shift from craton-derived, shallow-marine distal foreland (back-bulge) accumulation in the Mirador Formation, to orogen-sourced, deltaic, and coastalinfluenced sedimentation of the distal to medial foreland (foredeep) in the Carbonera and Leon Formations, to anastomosing fluvial and distributive braided fluvial megafan systems of the proximal foreland (foredeep to wedge-top) basin in the lower and upper Guayabo Formation. These changes in depositional processes and sediment dispersal are supported by up-section variations in detrital zircon U-Pb and (U-Th)/He ages that record exhumation of evolving, compartmentalized sediment source areas in the Eastern Cordillera. The data are interpreted in terms of a progressive eastward advance in foldand-thrust deformation, with late Eocene Oligocene deformation in the axial zone of the Eastern Cordillera along the western edge of Floresta basin (Soapaga thrust), early Miocene reactivation (inversion) of the eastern margin of the Mesozoic rift system (Pajarito and Guaicaramo thrusts), and middle late Miocene propagation of a footwall shortcut fault (Vopal thrust) that created the Nunchia syncline in a wedge-top (piggyback) setting of the eastern foothills along the transition from the Eastern Cordillera to Harms foreland basin. Collectively, the data presented here for the frontal Eastern Cordillera define a general in-sequence pattern of eastwardadvancing fold-and-thrust deformation during Cenozoic east-west shortening in the Colombian Andes.
The time-dependent approach to electronic spectroscopy, as popularized by Heller and co-workers in the 1980s, is applied here in conjunction with linear-response, time-dependent density functional theory to study vibronic absorption and resonance Raman spectra of beta-carotene, with and without a solvent. Two-state models, the harmonic and the Condon approximations are used in order to do so. A new code has been developed which includes excited state displacements, vibrational frequency shifts, and Duschinsky rotation, i.e., mode mixing, for both non-adiabatic spectroscopies. It is shown that Duschinsky rotation has a pronounced effect on the resonance Raman spectra of beta-carotene. In particular, it can explain a recently found anomalous behaviour of the so-called nu(1) peak in resonance Raman spectra [N. Tschirner, M. Schenderlein, K. Brose, E. Schlodder, M. A. Mroginski, C. Thomsen, and P. Hildebrandt, Phys. Chem. Chem. Phys. 11, 11471 (2009)], which shifts with the change in excitation wavelength.
Whereas the US President signed the Kyoto Protocol, the failure of the US Congress to ratify it seriously hampered subsequent international climate cooperation. This recent US trend, of signing environmental treaties but failing to ratify them, could thwart attempts to come to a future climate agreement. Two complementary explanations of this trend are proposed. First, the political system of the US has distinct institutional features that make it difficult for presidents to predict whether the Senate will give its advice and consent to multilateral environmental agreements (MEAs) and whether Congress will pass the required enabling legislation. Second, elected for a fixed term, US presidents might benefit politically from supporting MEAs even when knowing that legislative support is not forthcoming. Four policy implications are explored, concerning the scope for unilateral presidential action, the potential for bipartisan congressional support, the effectiveness of a treaty without the US, and the prospects for a deep, new climate treaty.
Policy relevance
Why does the failure of US ratification of multilateral environmental treaties occur? This article analyses the domestic political mechanisms involved in cases of failed US ratification. US non-participation in global environmental institutions often has serious ramifications. For example, it sharply limited Kyoto's effectiveness and seriously hampered international climate negotiations for years. Although at COP 17 in Durban the parties agreed to negotiate a new agreement by 2015, a new global climate treaty may well trigger a situation resembling the one President Clinton faced in 1997 when he signed Kyoto but never obtained support for it in the Senate. US failure to ratify could thwart future climate agreements.
Density Functional Calculations of the Anisotropic Effects of Borazine and 1,3,2,4-Diazadiboretidine
(2012)
On the basis of the nucleus-independent chemical shift (NICS) concept, the anisotropic effects of two inorganic rings, namely, borazine and planar 1,3,2,4-diazadiboretidine, are quantitatively calculated and visualized as isochemical shielding surfaces (ICSSs). Dissection of magnetic shielding values along the three Cartesian axes into contributions from s and p bonds by the natural chemical shieldingnatural bond orbital (NCSNBO) method revealed that their appearance is not a simple reflection of the extent of (anti)aromaticity.