Refine
Year of publication
- 2024 (383)
- 2023 (1207)
- 2022 (2611)
- 2021 (2369)
- 2020 (2684)
- 2019 (2470)
- 2018 (2665)
- 2017 (2399)
- 2016 (2217)
- 2015 (2001)
- 2014 (1680)
- 2013 (1715)
- 2012 (1791)
- 2011 (1713)
- 2010 (1196)
- 2009 (1537)
- 2008 (1129)
- 2007 (1089)
- 2006 (1474)
- 2005 (1629)
- 2004 (1737)
- 2003 (1373)
- 2002 (1208)
- 2001 (1274)
- 2000 (1544)
- 1999 (1746)
- 1998 (1598)
- 1997 (1470)
- 1996 (1497)
- 1995 (1426)
- 1994 (967)
- 1993 (367)
- 1992 (194)
- 1991 (143)
- 1989 (3)
- 1972 (1)
Document Type
- Article (32899)
- Doctoral Thesis (6451)
- Monograph/Edited Volume (5432)
- Postprint (2266)
- Review (1799)
- Part of a Book (1053)
- Other (941)
- Conference Proceeding (398)
- Part of Periodical (361)
- Preprint (260)
Language
- English (29686)
- German (22030)
- Spanish (310)
- French (264)
- Italian (111)
- Russian (71)
- Multiple languages (42)
- Hebrew (36)
- Portuguese (25)
- Polish (24)
Is part of the Bibliography
- yes (52632) (remove)
Keywords
- climate change (176)
- Germany (131)
- machine learning (90)
- diffusion (79)
- Deutschland (73)
- Arabidopsis thaliana (70)
- German (70)
- gender (62)
- anomalous diffusion (59)
- stars: massive (59)
Institute
- Institut für Biochemie und Biologie (5486)
- Institut für Physik und Astronomie (5367)
- Institut für Geowissenschaften (3667)
- Institut für Chemie (3417)
- Wirtschaftswissenschaften (2487)
- Department Psychologie (2232)
- Historisches Institut (2093)
- Sozialwissenschaften (1839)
- Institut für Mathematik (1834)
- Institut für Romanistik (1766)
There is increasing evidence linking the mass-extinction event at the Cretaceous-Paleogene boundary to an asteroid impact near Chicxulub, Mexico. Here we use model simulations to explore the combined effect of sulfate aerosols, carbon dioxide and dust from the impact on the oceans and the marine biosphere in the immediate aftermath of the impact. We find a strong temperature decrease, a brief algal bloom caused by nutrients from both the deep ocean and the projectile, and moderate surface ocean acidification. Comparing the modeled longer-term post-impact warming and changes in carbon isotopes with empirical evidence points to a substantial release of carbon from the terrestrial biosphere. Overall, our results shed light on the decades to centuries after the Chicxulub impact which are difficult to resolve with proxy data.
Plain Language Summary The sudden disappearance of the dinosaurs and many other species during the end-Cretaceous mass extinction 66 million years ago marks one of the most profound events in the history of life on Earth. The impact of a large asteroid near Chicxulub, Mexico, is increasingly recognized as the trigger of this extinction, causing global darkness and a pronounced cooling. However, the links between the impact and the changes in the biosphere are not fully understood. Here, we investigate how life in the ocean reacts to the perturbations in the decades and centuries after the impact. We find a short-lived algal bloom caused by the upwelling of nutrients from the deep ocean and nutrient input from the impactor.
Previous studies suggest that associations between numbers and space are mediated by shifts of visuospatial attention along the horizontal axis. In this study, we investigated the effect of vertical shifts of overt attention, induced by optokinetic stimulation (OKS) and monitored through eye-tracking, in two tasks requiring explicit (number comparison) or implicit (parity judgment) processing of number magnitude. Participants were exposed to black-and-white stripes (OKS) that moved vertically (upward or downward) or remained static (control condition). During the OKS, participants were asked to verbally classify auditory one-digit numbers as larger/smaller than 5 (comparison task; Exp. 1) or as odd/even (parity task; Exp. 2). OKS modulated response times in both experiments. In Exp.1, upward attentional displacement decreased the Magnitude effect (slower responses for large numbers) and increased the Distance effect (slower responses for numbers close to the reference). In Exp.2, we observed a complex interaction between parity, magnitude, and OKS, indicating that downward attentional displacement slowed down responses for large odd numbers. Moreover, eye tracking analyses revealed an influence of number processing on eye movements both in Exp. 1, with eye gaze shifting downwards during the processing of small numbers as compared to large ones; and in Exp. 2, with leftward shifts after large even numbers (6,8) and rightward shifts after large odd numbers (7,9). These results provide evidence of bidirectional links between number and space and extend them to the vertical dimension. Moreover, they document the influence of visuo-spatial attention on processing of numerical magnitude, numerical distance, and parity. Together, our findings are in line with grounded and embodied accounts of numerical cognition.
Forage supply of savanna grasslands plays a crucial role for local food security and consequently, a reliable monitoring system could help to better manage vital forage resources. To help installing such a monitoring system, we investigated whether in-situ hyperspectral data could be resampled to match the spectral resolution of multi- and hyperspectral satellites; if the type of sensor affected model transfer; and if spatio-temporal patterns of forage characteristics could be related to environmental drivers. We established models for forage quantity (green biomass) and five forage quality proxies (metabolisable energy, acid/neutral detergent fibre, ash, phosphorus). Hyperspectral resolution of the Hyperion satellite mostly resulted in higher accuracies (i.e. higher R-2, lower RMSE). When applied to satellite data, though, the greater quality of the multispectral Sentinel-2 satellite data leads to more realistic forage maps. By analysing a three-year time series, we found plant phenology and cumulated precipitation to be the most important environmental drivers of forage supply. We conclude that none of the investigated satellites provide optimal conditions for monitoring purposes. Future hyperspectral satellite missions like EnMAP, combining the high information level of Hyperion with the good data quality and resolution of Sentinel-2, will provide the prerequisites for installing a regular monitoring service.
The history of the Messiah in Judaism is a history of disappointed hopes. Again and again, there were salvation fi gures to whom this role was ascribed. But redemption from occupation and foreign rule, exile, oppression and persecution failed to materialize. Therefore, the expectation of the Messiah fell to the periphery of Jewish theology. This article examinesin what ways the messianic concept plays a role in modern times and what it contributes to describing the relationship between God and humanity in Judaism. The author intends to show the development from the abandonment of a personal Messiah towards the affi rmation of the prophets’ hope for a universal messianic age in which the duty of all people to participate in the healing of the world becomes central. What becomes also clear is: The messiah idea cannot be a bridge between Christianity and Judaism.
Rhythmicity characterizes both interpersonal synchrony and spoken language. Emotions and language are forms of interpersonal communication, which interact with each other throughout development. We investigated whether and how emotional synchrony between mothers and their 9-month-old infants relates to infants' word segmentation as an early marker of language development. Twenty-six 9-month-old infants and their German-speaking mothers took part in the study. To measure emotional synchrony, we coded positive, neutral and negative emotional expressions of the mothers and their infants during a free play session. We then calculated the degree to which the mothers' and their infants' matching emotional expressions followed a predictable pattern. To measure word segmentation, we familiarized infants with auditory text passages and tested how long they looked at the screen while listening to familiar versus novel words. We found that higher levels of predictability (i.e. low entropy) during mother-infant interaction is associated with infants' word segmentation performance. These findings suggest that individual differences in word segmentation relate to the complexity and predictability of emotional expressions during mother-infant interactions.
Kein Messias in Sicht
(2024)
In You Shall Be as Gods, Erich Fromm (1900–1980) defines his position as nontheistic mysticism. This research clarifies the term, considers its importance within Fromm’s humanism, and explores its potential origins. The nontheistic mystical position plays a central role in Fromm’s understanding of the relationship between mysticism and organized religion, religion and religiosity, and it clarifies the relationship between religion, philosophy, and social psychoanalysis, whose combination constitutes his humanistic ethics. Nontheistic mysticism relates, as well, to Fromm’s understanding of human nature; it involves the question of the relationship between language, perception, and experience. The nontheistic mystical position is linked to Fromm’s negative theology, the x experience, and idolatry. Hence, the nontheistic mystical position is relevant to Fromm’s understanding of self-realization and his vision of a sane society. Unlike some scholarly opinion, the conclusions of this paper suggest that Fromm’s humanism is not radical, as long as radical is defined as an absolute atheistic secular feature that eliminates the range of religious language and experience. Rather, it is a broad and cautious humanism that, on the one hand, internalizes the transcendent divinity into the human subject and transforms it into anthropological–ethical phenomena, but, on the other, implies that atheism carries the risk of an idolatrous identification of the human being with God. Consequently, this humanism requires a religious–mystical component to adequately portray the spiritual and ethical potentials of humanity and its challenges. Nontheistic mysticism is a consciousness mechanism aimed at the fine-tuning of the individual’s moral compass, which is affected by the pathologies of normalcy that prevail in all societies.
Convolutional neural networks (CNNs) have been used for a wide range of applications in astronomy, including for the restoration of degraded images using a spatially invariant point spread function (PSF) across the field of view. Most existing development techniques use a single PSF in the deconvolution process, which is unrealistic when spatially variable PSFs are present in real observation conditions. Such conditions are simulated in this work to yield more realistic data samples. We propose a method that uses a simulated spatially variable PSF for the T80-South (T80-S) telescope, an 80-cm survey imager at Cerro Tololo (Chile). The synthetic data use real parameters from the detector noise and atmospheric seeing to recreate the T80-S observational conditions for the CNN training. The method is tested on real astronomical data from the T80-S telescope. We present the simulation and training methods, the results from real T80-S image CNN prediction, and a comparison with space observatory Gaia. A CNN can fix optical aberrations, which include image distortion, PSF size and profile, and the field position variation while preserving the source's flux. The proposed restoration approach can be applied to other optical systems and to post-process adaptive optics static residual aberrations in large-diameter telescopes.
This longitudinal study aimed to investigate how motivational transitions of adolescents in the domain of mathematics from Grades 9 to 10 were related to student-perceived mathematics teacher support and student -oriented teaching. Data were drawn from the 2012 Programme for International Student Assessment (PISA) and its German national extension called PISA Plus 2012-2013. We used a subsample of 2605 students (51.0 % girls) from 198 classrooms. Using latent profile analyses, we identified three motivational patterns based on expectancy-value theory that were meaningfully associated with students' mathematics test scores and work ethics. Latent transition analyses showed that these patterns were mostly stable across time. Occurring changes were characterized by a decrease in mathematics motivation across time. Student-oriented teaching as reported by students in Grade 9 impeded maladaptive motivational transitions. Students with particularly low interest and utility value benefitted from teachers who direct their instruction at students' motivational characteristics.
Purpose: Coarticulatory effects in speech vary across development, but the sources of this variation remain unclear. This study investigated whether developmental differences in intrasyllabic coarticulation degree could be explained by differences in children's articulatory patterns compared to adults.
Method: To address this question, we first compared the tongue configurations of 3-to 7-year-old German children to those of adults. The observed developmental differences were then examined through simulations with Task Dynamics Application, a Task Dynamics simulation system, to establish which articulatory modifications could best reproduce the empirical results. To generate syllables simulating the lack of tongue gesture differentiation, we tested three simulation scenarios.
Results: We found that younger speakers use less differentiated articulatory patterns to achieve alveolar constrictions than adults. The simulations corresponding to undifferentiated control of tongue tip and tongue body resulted in (a) tongue shapes similar to those observed in natural speech and (b) higher degrees of intrasyllabic coarticulation in children when compared to adults.
Conclusions: Results provide evidence that differences in articulatory patterns contribute to developmental differences in coarticulation degree. This study further shows that empirically informed modeling can advance our understanding of changes in coarticulatory patterns across age.
The article explores the philosophical exegesis in Obadiah Sforno's sixteenth-century Psalms commentary and its reception in Berlin of the late eighteenth century, where it was reprinted in the Haskalah's biggest bestseller-an edition of Moses Mendelssohn's Psalms translation with Hebrew commentary.
While the inclusion of entire commentaries by earlier exegetes was unique among all Haskalah Bible editions, I argue that the choice to include Sforno's commentary alongside Mendelssohn's translation of Psalms, itself an expression of Mendelssohn's political-theological defense of Judaism, was intended to buttress shared philosophical doctrines and concepts located by the two scholars in Psalms, notwithstanding temporal and cultural divergences: imitatio Dei, the salvation of the individual soul, and Israel's eternity.
In this work, we present a comprehensive evaluation of a stochastic multi-site, multi-variate weather generator at the scale of entire Germany and parts of the neighbouring countries covering the major German river basins Elbe, Upper Danube, Rhine, Weser and Ems with a total area of approximately 580,000 km(2). The regional weather generator, which is based on a first-order multi-variate auto-regressive model, is setup using 53-year long daily observational data at 528 locations. The performance is evaluated by investigating the ability of the weather generator to replicate various important statistical properties of the observed variables including precipitation occurrence and dry/wet transition probabilities, mean daily and extreme precipitation, multi-day precipitation sums, spatial correlation structure, areal precipitation, mean daily and extreme temperature and solar radiation. We explore two marginal distributions for daily precipitation amount: mixed Gamma-Generalized Pareto and extended Generalized Pareto. Furthermore, we introduce a new procedure to estimate the spatial correlation matrix and model mean daily temperature and solar radiation. The extensive evaluation reveals that the weather generator is greatly capable of capturing most of the crucial properties of the weather variables, particularly of extreme precipitation at individual locations. Some deficiencies are detected in capturing spatial precipitation correlation structure that leads to an overestimation of areal precipitation extremes. Further improvement of the spatial correlation structure is envisaged for future research. The mixed marginal model found to outperform the extended Generalized Pareto in our case. The use of power transformation in combination with normal distribution significantly improves the performance for non-precipitation variables. The weather generator can be used to generate synthetic event footprints for large-scale trans-basin flood risk assessment.
More than 30 years have passed since Mehler et al. (1988) proposed that newborns can discriminate between languages that belong to different rhythm classes: stress-, syllable- or mora-timed. Thereupon they developed the hypothesis that infants are sensitive to differences in vowel and consonant interval durations as acoustic correlates of rhythm classes. It remains unknown exactly which durational computations infants use when perceiving speech for the purposes of distinguishing languages. Here, a meta-analysis of studies on infants' language discrimination skills over the first year of life was conducted, aiming to quantify how language discrimination skills change with age and are modulated by rhythm classes or durational metrics. A systematic literature search identified 42 studies that tested infants' (birth to 12 months) discrimination or preference of two language varieties, by presenting infants with auditory or audio-visual continuous speech. Quantitative data synthesis was conducted using multivariate random effects meta-analytic models with the factors rhythm class difference, age, stimulus manipulation, method, and metrics operationalising proportions of and variability in vowel and consonant interval durations, to explore which factors best account for language discrimination or preference. Results revealed that smaller differences in vowel interval variability (oV) and larger differences in successive consonantal interval variability (rPVI-C) were associated with more successful language discrimination, and better accounted for discrimination results than the factor rhythm class. There were no effects of age for discrimination but results on preference studies were affected by age: the older infants get, the more they prefer non-native languages that are rhythmically similar to their native language, but not non-native languages that are rhythmically distinct. These findings can inform theories on language discrimination that have previously focussed on rhythm class, by providing a novel way to operationalise rhythm in language in the extent to which it accounts for infants' language discrimination abilities.
Temperature is an important factor governing microbe-mediated carbon feedback from permafrost soils. The link between taxonomic and functional microbial responses to temperature change remains elusive due to the lack of studies assessing both aspects of microbial ecology. Our previous study reported microbial metabolic and trophic shifts in response to short-term temperature increases in Arctic peat soil, and linked these shifts to higher CH4 and CO2 production rates (Proceedings of the National Academy of Sciences of the United States of America, 112, E2507-E2516). Here, we studied the taxonomic composition and functional potential of samples from the same experiment. We see that along a high-resolution temperature gradient (1-30 degrees C), microbial communities change discretely, but not continuously or stochastically, in response to rising temperatures. The taxonomic variability may thus in part reflect the varied temperature responses of individual taxa and the competition between these taxa for resources. These taxonomic responses contrast the stable functional potential (metagenomic-based) across all temperatures or the previously observed metabolic or trophic shifts at key temperatures. Furthermore, with rising temperatures we observed a progressive decrease in species diversity (Shannon Index) and increased dispersion of greenhouse gas (GHG) production rates. We conclude that the taxonomic variation is decoupled from both the functional potential of the community and the previously observed temperature-dependent changes in microbial function. However, the reduced diversity at higher temperatures might help explain the higher variability in GHG production at higher temperatures.
Trade-offs between traits are present across different levels of biological systems and ultimately reflect constraints imposed by physicochemical laws and the structure of underlying biochemical networks. Yet, mechanistic explanation of how trade-offs between molecular traits arise and how they relate to optimization of fitness-related traits remains elusive. Here, we introduce the concept of relative flux trade-offs and propose a constraint-based approach, termed FluTOr, to identify metabolic reactions whose fluxes are in relative trade-off with respect to an optimized fitness-related cellular task, like growth. We then employed FluTOr to identify relative flux trade-offs in the genome-scale metabolic networks of Escherichia coli, Saccharomyces cerevisiae, and Arabidopsis thaliana. For the metabolic models of E. coli and S. cerevisiae we showed that: (i) the identified relative flux trade-offs depend on the carbon source used and that (ii) reactions that participated in relative trade-offs in both species were implicated in cofactor biosynthesis. In contrast to the two microorganisms, the relative flux trade-offs for the metabolic model of A. thaliana did not depend on the available nitrogen sources, reflecting the differences in the underlying metabolic network as well as the considered environments. Lastly, the established connection between relative flux trade-offs allowed us to identify overexpression targets that can be used to optimize fitness-related traits. Altogether, our computational approach and findings demonstrate how relative flux trade-offs can shape optimization of metabolic tasks, important in biotechnological applications.
Formal constraints on crossing dependencies have played a large role in research on the formal complexity of natural language grammars and parsing. Here we ask whether the apparent evidence for constraints on crossing dependencies in treebanks might arise because of independent constraints on trees, such as low arity and dependency length minimization. We address this question using two sets of experiments. In Experiment 1, we compare the distribution of formal properties of crossing dependencies, such as gap degree, between real trees and baseline trees matched for rate of crossing dependencies and various other properties. In Experiment 2, we model whether two dependencies cross, given certain psycholinguistic properties of the dependencies. We find surprisingly weak evidence for constraints originating from the mild context-sensitivity literature (gap degree and well-nestedness) beyond what can be explained by constraints on rate of crossing dependencies, topological properties of the trees, and dependency length. However, measures that have emerged from the parsing literature (e.g., edge degree, end-point crossings, and heads' depth difference) differ strongly between real and random trees. Modeling results show that cognitive metrics relating to information locality and working-memory limitations affect whether two dependencies cross or not, but they do not fully explain the distribution of crossing dependencies in natural languages. Together these results suggest that crossing constraints are better characterized by processing pressures than by mildly context-sensitive constraints.
Innate or acquired?
(2021)
In this chapter, some of the findings from sociolinguistic interviews with 25 speakers of Indian English and 26 speakers of Nigerian English are presented. Emanating from a larger research project concerned with conceptualizations of gender, the current analysis focuses on conceptualizations of homosexuality and makes use of the analytical tools provided by Cultural Linguistics and Cognitive Sociolinguistics. In particular, the notions of “cultural conceptualizations” (e.g., Sharifian, 2011, 2017) and “cultural model” (e.g., Wolf & Polzenhagen, 2009; also cf. Schneider, 2014) are addressed.
At the time of data collection, discriminatory legislation concerning homosexuality was in force in India and Nigeria. Opinion polls likewise echoed a negative stance towards homosexuality among the population of the two countries. This raised the expectation that similar conceptualizations of homosexuality might be found in Indian and Nigerian English, both in terms of their negative connotation and of how homosexuality would exactly be conceptualized. However, this expectation was not fulfilled. Firstly, the acceptance among the Indian participants to this study was generally greater. Secondly, homosexuality was predominantly conceptualized as an innate condition in the Indian English data, while it was prevalently understood as an acquired condition by the Nigerian informants. Drawing from earlier findings within the context of the same project (Finzel, 2021; fc.), I suggest that these differences can be explained with culture-specific models of gender that lend their logic to conceptualizations of homosexuality.
Развитие науки, общества и медицины ведет за собой неуклонный рост средней продолжительности жизни. В результате наука сталкивается с новыми задачами, в число которых входит предотвращение деменции и поддержание эффективной работоспособности мозга при старении человека. Одним из защитных факторов, предотвращающих ослабление когнитивных функций и поддерживающих нейропластичность мозга, является билингвизм.
Однако дебаты насчет нейропротекторных свойств двуязычия до сих пор являются актуальными. В данной статье рассмотрен феномен нейрокогнитивного резерва (НКР) и определена взаимосвязь между билингвизмом и такими составляющими НКР, как мозговой резерв, нейрональный резерв и нейрональная компенсация.
Кроме этого, в статье приведен обзор исследований, посвященных изучению нейронных и когнитивных механизмов влияния билингвизма на здоровое функционирование мозга при старении. В заключении мы вкратце остановились на том, каким образом двуязычие может защищать мозг от старения благодаря взаимодействию между мозговыми и когнитивными составляющими НКР.
Most, if not all, of the studies in Cultural Linguistics have (a) taken a synchronic perspective or (b) focused on specific, intracultural conceptualizations. In my chapter, I will look at a cluster of conceptualizations that have been found to exist in different historical periods, in different languages and varieties, and on different continents. The case in point is conceptualizations of magical practices based on menstrual blood. The existence of these conceptualizations across time and space raises the challenging questions of their motivation, and, more generally, the “flow of conceptualizations.” While these questions will be pursued in my chapter, the main focus will be on an elaboration of the conceptual network of conceptualizations pertaining to menstrual blood magic.
Microbiomes have an immense potential to enhance plant resilience to various biotic and abiotic stresses. However, intrinsic microbial communities respond to changes in their host's physiology and environment during plant's life cycle. The potential of the inherent plant microbiome has been neglected for a long time, especially for the postharvest period. Currently, close to 50% of all produced fruits and vegetables are lost either during production or storage. Biological control of spoilage and storage diseases is still lacking sufficiency. Today, novel multiomics technologies allow us to study the microbiome and its responses on a community level, which will help to advance current classic approaches and develop more effective and robust microbiome-based solutions for fruit and vegetable storability, quality, and safety.
Otter shrew mitogenomes (Afrotheria, Potamogalidae) reconstructed from historical museum skins
(2022)
African otter shrews (Potamogalidae) are Afrotherian mammals adapted to a semi-aquatic lifestyle. Given their rareness, genetic data on otter shrews are limited. By applying laboratory methods tuned for the recovery of archival DNA and an iterative mapping approach, we reconstructed whole mitochondrial genomes of the Giant (Potamogale velox) and Ruwenzori pygmy otter shrew (Micropotamogale ruwenzorii) from historical museum skins. Phylogenetic analyses are consistent with previous reports in recovering a sister relationship between African otter shrews and Malagasy tenrecs. The long branches separating both lineages, however, support their recognition as separate families.
Critical role of parasite-mediated energy pathway on community response to nutrient enrichment
(2022)
Parasites form an integral part of food webs, however, they are often ignored in classic food web theory or limited to the investigation of trophic transmission pathways. Specifically, direct consumption of parasites by nonhost predators is rarely considered, while it can contribute substantially to energy flow in food webs. In aquatic systems, chytrids constitute a major group of fungal parasites whose free-living infective stages (zoospores) form a highly nutritional food source to zooplankton. Thereby, the consumption of zoospores can create an energy pathway from otherwise inedible phytoplankton to zooplankton ( "mycoloop "). This parasite-mediated energy pathway might be of special importance during phytoplankton blooms dominated by inedible or toxic primary producers like cyanobacteria, which are on the rise with eutrophication and global warming. We theoretically investigated community dynamics and energy transfer in a food web consisting of an edible nonhost and an inedible host phytoplankton species, a parasitic fungus, and a zooplankton species grazing on edible phytoplankton and fungi. Food web dynamics were investigated along a nutrient gradient contrasting nonadaptive zooplankton species representative for filter feeders like cladocerans and zooplankton with the ability to actively adapt their feeding preferences like many copepod species. Overall, the importance of the mycoloop for zooplankton increases with nutrient availability. This increase is smooth for nonadaptive consumers. For adaptive consumers, we observe an abrupt shift from an almost exclusive preference for edible phytoplankton at low nutrient levels to a strong preference for parasitic fungi at high nutrient levels. The model predicts that parasitic fungi could contribute up to 50% of the zooplankton diet in nutrient-rich environments, which agrees with empirical observations on zooplankton gut content from eutrophic systems during blooms of inedible diatoms or cyanobacteria. Our findings highlight the role of parasite-mediated energy pathways for predictions of energy flow and community composition under current and future environmental change.
Ethik-Komitees gehören zum festen Bestandteil des Ethikmanagements und der Organisationsethik in klinischen Einrichtungen des Gesundheitswesens. Entsprechende Ethikstrukturen und die damit verbundenen Angebote stoßen hinsichtlich ihrer Wirksamkeit allerdings an ihre Grenzen. Ihre Arbeitsweisen sind häufig reaktiv und eine Verankerung in den entsprechenden Organisationsebenen fehlt. Ausgehend von diesen Limitationen der klinischen Ethikberatung hat sich die multiprofessionelle „Arbeitsgruppe Ethik“ am Universitätsklinikum Tübingen (UKT) um die Konzeption und Implementierung eines neuen Ansatzes zur nachhaltigen Integration von ethischen Reflexions- und Entscheidungsprozessen auf den Stationen des UKT bemüht. Mit dem Tübinger Modell der Ethikbeauftragten der Station verfolgt sie ein Pilotprojekt, das speziell geschulte Pflegekräfte aus allen Stationen des UKT als AnsprechpartnerInnen für ethische Fragen einsetzt. Damit stellen die Ethikbeauftragten eine Erweiterung zu etablierten Strukturen der Ethikberatung dar und ergänzen vorhandene Top-Down-Strategien. Der vorliegende Beitrag stellt die Zielsetzungen des Tübinger Modells dar und schildert erste Erfahrungen in der Umsetzung. Neben der Einbettung in organisationale Strukturen der Ethikberatung werden die stationsinternen und stationsübergreifenden Aufgaben der Ethikbeauftragten dargestellt. Zudem wird das Qualifikationsprogramm für Ethikbeauftragte (Basis- und Aufbauschulung) sowie ein Train-the-Trainer-Konzept vorgestellt, welche eine vertiefende Entwicklung von pflege- und medizinethischer Kompetenzen unterstützen und Sicherheit in den stationsbezogenen Reflexions- und Entscheidungsprozessen vermitteln.
Multi-species biofilms are more resistant against stress compared to single-species biofilms. However, the mechanisms underlying this common observation remain elusive. Therefore, we studied biofilm formation of well-known opportunistic pathogens (Acinetobacter baumanii, Enterococcus faecium, Escherichia coli, Staphylococcus haemolyticus and Stenotrophomonas maltophilia) in various approaches. Synergistic effects in their multi species biofilms were observed. Using metatranscriptomics, changes in the gene expression of the involved members became evident, and provided explanations for the improved survivability under nutrient limitation and exposure to disinfectants. Genes encoding proteins for vitamin B6 synthesis and iron uptake were linked to synergism in the multi-species biofilm under nutrient-limited conditions. Our study indicates that sub-lethal concentrations of an alcohol-based disinfectant enhance biofilm yields in multi-species assemblages. A reduction of the dominant taxa in the multi-species biofilm under disinfectant pressure allowed minor taxa to bloom. The findings underline the importance of minor but antimicrobial-resistant species that serve as "protectors" for the whole assemblage due to upregulation of genes involved in defence mechanisms and biofilm formation. This ultimately results in an increase in the total yield of the multi-species biofilm. We conclude that inter-species interactions may be crucial for the survival of opportunistic pathogens; especially under conditions that are typically found under hospital settings.
The acquisition of clitics still remains a highly controversial issue in Greek acquisition literature despite the bulk of studies performed.
Object clitics have been shown to be early acquired by monolingual children in terms of production rates, whereas only highly proficient bilingual children achieve target-like performance.
Crucially, errors in gender marking are persistent for monolingual and bilingual children even when adult-like production rates are achieved.
This study aims to readdress the acquisition of clitics in an innovative way, by entering the variable of gender in an experimental design targeting to assess production and processing by bilingual and monolingual children.
Moreover, we examined the role of language proficiency (in terms of general verbal intelligence and syntactic production abilities). The groups had comparable performance in both tasks (in terms of correct responses and error distribution in production and reaction times in comprehension).
However, verbal intelligence had an effect on the performance of the monolingual but not of the bilingual group in the production task, and bilingual children were overall slower in the comprehension task. Syntactic production abilities did not have any effect. We argue that gender marking affects clitic processing, and we discuss the implications of our findings for bilingual acquisition.
A marine sediment record from the central Bering Sea, spanning the last 20 thousand years (ka), was studied to unravel the depositional history with regard to terrigenous sediment supply and biogenic sedimentation. Methodic approaches comprised the inference of accumulation rates of siliciclastic and biogenic components, grain-size analysis, and (clay) mineralogy, as well as paleoclimatic modelling. Changes in the depositional history provides insight into land-ocean linkages of paleoenvironmental changes. During the finale of the Last Glacial Maximum, the depositional environment was characterized by hemipelagic background sedimentation. A marked change in the terrigenous sediment provenance during the late Heinrich 1 Stadial (15.7-14.5 ka), indicated by increases in kaolinite and a high glaciofluvial influx of clay, gives evidence of the deglaciation of the Brooks Range in the hinterland of Alaska. This meltwater pulse also stimulated the postglacial onset of biological productivity. Glacial melt implies regional climate warming during a time of widespread cooling on the northern hemisphere. Our simulation experiment with a coupled climate model suggests atmospheric teleconnections to the North Atlantic, with impacts on the dynamics of the Aleutian Low system that gave rise to warmer winters and an early onset of spring during that time. The late deglacial period between 14.5 and 11.0 ka was characterized by enhanced fluvial runoff and biological productivity in the course of climate amelioration, sea-level rise, seasonal sea-ice retreat, and permafrost thaw in the hinterland. The latter processes temporarily stalled during the Younger Dryas stadial (12.9-11.7 ka) and commenced again during the Preboreal (earliest Holocene), after 11.7 ka. High river runoff might have fertilized the Bering Sea and contributed to enhanced upper ocean stratification. Since 11.0 ka, advanced transgression has shifted the coast line and fluvial influence of the Yukon River away from the study site. The opening of the Bering Strait strengthened contour currents along the continental slope, leaving behind winnowed sand-rich sediments through the early to mid-Holocene, with non-deposition occurring since about 6.0 ka.
Rapid humidity changes across the Northern South China Sea during the last similar to 40 kyrs
(2021)
A key aspect of East Asian climate is its summer monsoonal system which influences nearly one-third of the world's population. Recent results indicate that the primary response of the East Asian summer monsoon (EASM) to anthropogenic forced climate warming may be a shift in geographical range instead of an intensity change, which would lead to spatial coexistence of floods and droughts over southeastern Asia. The predicted EASM variability in the future has made it paramount to study its past changes and the associated tempo-spatial pattern of aridity and humidity in its purview. In order to decipher past changes in EASM, we applied a multi-proxy geochemical approach to the sediment core ORI-891-16-P1 located in the northern South China Sea. The position of this sediment core on top of a seamount makes it uniquely sensitive to changes in the terrigenous input into northern South China Sea unbiased by sea level-induced downslope transport processes. Utilizing the ln(Ti/Ca) ratio throughout the sediment sequence we trace terrigenous influx changes reflecting EASM prevalence during the last similar to 40 kyrs. Based on the comparison of our results to previous studies we infer that the Last Glacial Maximum (LGM; similar to 20 ka BP) was characterized by a steep N-S humidity gradient. This spatial pattern was in line with a southward shift or contraction of the summer monsoonal trough of 10-15 degrees from its current position toward the centre of the South China Sea. Superimposed on orbital time scale fluctuations we also find strong indication of millennial-scale variability related to Heinrich Stadials. The impact of Heinrich Stadials on the EASM seems amplified during insolation minima, while high summer insolation seems to buffer the monsoonal system to such perturbations. We infer that (i) the humidity-aridity distribution during the LGM mimics predictions of the proposed future EASM configuration, and (ii) that the sensitivity of the EASM to weakening in the Atlantic Meridional Overturning Circulation is the strongest since the last glacial.
We consider the synchrotron emission from electrons out in the Galactic halo bubble region where the Fermi bubble structures reside.
Utilizing a simple analytical expression for the non-thermal electron distribution and a toy magnetic field model, we simulate polarized synchrotron emission maps at a frequency of 30 GHz.
Comparing these maps with the observational data, we obtain constraints on the parameters of our toy Galactic halo bubble magnetic field model.
Utilizing this parameter value range for the toy magnetic field model, we determine the corresponding range of arrival directions and suppression factors of ultra high energy cosmic rays (UHECRs) from potential local source locations.
We find that high levels of flux suppression (down to 2 per cent) and large deflection angles (>= 80 degrees) are possible for source locations whose line of sight pass through the Galactic halo bubble region.
We conclude that the magnetic field out in the Galactic halo bubble region can strongly dominate the level of deflection UHECRs experience whilst propagating from local sources to Earth.
Large agricultural streams receive excessive inputs of nitrogen.
However, quantifying the role of these streams in nitrogen processing remains limited because continuous direct measurements of the interacting and highly time-varying nitrogen processing pathways in larger streams and rivers are very complex.
Therefore, we employed a monitoring-driven modelling approach with high-frequency in situ data and the river water quality model Water Quality Analysis Simulation Program (WASP) 7.5.2 in the 27.4 km reach of the sixth-order agricultural stream called Lower Bode (central Germany) for a 5-year period (2014-2018).
Paired high-frequency sensor data (15 min interval) of discharge, nitrate, dissolved oxygen, and chlorophyll a at upstream and downstream stations were used as model boundaries and for setting model constraints.
The WASP model simulated 15 min intervals of discharge, nitrate, and dissolved oxygen with Nash-Sutcliffe efficiency values higher than 0.9 for calibration and validation, enabling the calculation of gross and net dissolved inorganic nitrogen uptake and pathway rates on a daily, seasonal, and multiannual scale.
Results showed daily net uptake rate of dissolved inorganic nitrogen ranged from -17.4 to 553.9 mgNm(-2)d(-1). The highest daily net uptake could reach almost 30 % of the total input loading, which occurred at extreme low flow in summer 2018.
The growing season (spring and summer) accounted for 91 % of the average net annual uptake of dissolved inorganic nitrogen in the measured period. In spring, both the DIN gross and net uptake were dominated by the phytoplankton uptake pathway. In summer, benthic algae assimilation dominated the gross uptake of dissolved inorganic nitrogen.
Conversely, the reach became a net source of dissolved inorganic nitrogen with negative daily net uptake values in autumn and winter, mainly because the release from benthic algae surpassed uptake processes.
Over the 5 years, average gross and net uptake rates of dissolved inorganic nitrogen were 124.1 and 56.8 mgNm(-2)d(-1), which accounted for only 2.7 % and 1.2 % of the total loadings in the Lower Bode, respectively. The 5-year average gross DIN uptake decreased from assimilation by benthic algae through assimilation by phytoplankton to denitrification.
Our study highlights the value of combining river water quality modelling with high-frequency data to obtain a reliable budget of instream dissolved inorganic nitrogen processing which facilitates our ability to manage nitrogen in aquatic systems.
This study provides a methodology that can be applied to any large stream to quantify nitrogen processing pathway dynamics and complete our understanding of nitrogen cycling.
Temperature impacts on hate speech online: evidence from 4 billion geolocated tweets from the USA
(2022)
Background - A link between weather and aggression in the offline world has been established across a variety of societal settings. Simultaneously, the rapid digitalisation of nearly every aspect of everyday life has led to a high frequency of interpersonal conflicts online. Hate speech online has become a prevalent problem that has been shown to aggravate mental health conditions, especially among young people and marginalised groups.
We examine the effect of temperature on the occurrence of hate speech on the social media platform Twitter and interpret the results in the context of the interlinkage between climate change, human behaviour, and mental health.
Methods - In this quantitative empirical study, we used a supervised machine learning approach to identify hate speech in a dataset containing around 4 billion geolocated tweets from 773 cities across the USA between May 1, 2014 and May 1, 2020.
We statistically evaluated the changes in daily hate tweets against changes in local temperature, isolating the temperature influence from confounding factors using binned panel-regression models.
Findings - The prevalence of hate tweets was lowest at moderate temperatures (12 to 21?) and marked increases in the number of hate tweets were observed at hotter and colder temperatures, reaching up to 12middot5% (95% CI 8middot0-16middot5) for cold temperature extremes (-6 to -3?) and up to 22middot0% (95% CI 20middot5-23middot5) for hot temperature extremes (42 to 45?). Outside of the moderate temperature range, the hate tweets also increased as a proportion of total tweeting activity. The quasi-quadratic shape of the temperature-hate tweet curve was robust across varying climate zones, income quartiles, religious and political beliefs, and both city-level and state-level aggregations.
However, temperature ranges with the lowest prevalence of hate tweets were centred around the local temperature mean and the magnitude of the increases in hate tweets for hot and cold temperatures varied across the climate zones.
Interpretation - Our results highlight hate speech online as a potential channel through which temperature alters interpersonal conflict and societal aggression. We provide empirical evidence that hot and cold temperatures can aggravate aggressive tendencies online. The prevalence of the results across climatic and socioeconomic subgroups points to limitations in the ability of humans to adapt to temperature extremes.
Soil microbial communities are crucial for plant growth and are already depleted by anthropogenic activities.
The application of microbial transplants provides a strategy to restore beneficial soil traits, but less is known about the microbiota of traditional inoculants used in biodynamic agriculture.
In this study, we used amplicon sequencing and quantitative PCR to decipher microbial communities of composts, biodynamic manures, and plant preparations from Austria and France.
In addition, we investigated the effect of extracts derived from biodynamic manure and compost on the rhizosphere microbiome of apple trees. Microbiota abundance, composition, and diversity of biodynamic manures, plant preparations, and composts were distinct. Microbial abundances ranged between 1010-1011 (bacterial 16S rRNA genes) and 109-1011 (fungal ITS genes). The bacterial diversity was significantly higher in biodynamic manures compared to compost without discernible differences in abundance. Fungal diversity was not significantly different while abundance was increased in biodynamic manures. The microbial communities of biodynamic manures and plant preparations were specific for each production site, but all contain potentially plant-beneficial bacterial genera.
When applied in apple orchards, biodynamic preparations (extracts) had the non-significant effect of reducing bacterial and fungal abundance in apple rhizosphere (4 months post-application), while increasing fungal and lowering bacterial Shannon diversity.
One to four months after inoculation, individual taxa indicated differential abundance. We observed the reduction of the pathogenic fungus Alternaria, and the enrichment of potentially beneficial bacterial genera such as Pseudomonas.
Our study paves way for the science-based adaptation of empirically developed biodynamic formulations under different farming practices to restore the vitality of agricultural soils.
Das Erbe der Aufklärung
(2024)
Patient monitoring technology has been used to guide therapy and alert staff when a vital sign leaves a predefined range in the intensive care unit (ICU) for decades. However, large amounts of technically false or clinically irrelevant alarms provoke alarm fatigue in staff leading to desensitisation towards critical alarms.
With this systematic review, we are following the Preferred Reporting Items for Systematic Reviews (PRISMA) checklist in order to summarise scientific efforts that aimed to develop IT systems to reduce alarm fatigue in ICUs. 69 peer-reviewed publications were included. The majority of publications targeted the avoidance of technically false alarms, while the remainder focused on prediction of patient deterioration or alarm presentation.
The investigated alarm types were mostly associated with heart rate or arrhythmia, followed by arterial blood pressure, oxygen saturation, and respiratory rate.
Most publications focused on the development of software solutions, some on wearables, smartphones, or headmounted displays for delivering alarms to staff.
The most commonly used statistical models were tree-based. In conclusion, we found strong evidence that alarm fatigue can be alleviated by IT-based solutions.
However, future efforts should focus more on the avoidance of clinically non-actionable alarms which could be accelerated by improving the data availability.
One of the most challenging difficulties for incumbent organisations, especially small- and medium-sized enterprises (SMEs), is to manage digital transformation driven by technological change. Incumbent organisations' responses to digital transformation have been extensively studied in the current literature.
However, most research neglects digital transformation in SMEs. There are hardly any valid developed measures for the maturity of digital transformation. We present a holistic digital transformation maturity model based on an extensive literature review, qualitative computer-assisted data analysis, and empirical findings.
The digital transformation maturity model focuses on small- and medium-sized enterprises' unique features and characteristics.
We proved the practical applicability and relevance of the digital transformation maturity model in an extensive study involving various organisations, particularly German SMEs (n = 310).
Organisations can use this model to assess themselves initially and, through this process, gain a comprehensive understanding of the multiple forms of digital transformation.
It is often claimed that the entropy of a network's degree distribution is a proxy for its robustness. Here, we clarify the link between degree distribution entropy and giant component robustness to node removal by showing that the former merely sets a lower bound to the latter for randomly configured networks when no other network characteristics are specified. Furthermore, we show that, for networks of fixed expected degree that follow degree distributions of the same form, the degree distribution entropy is not indicative of robustness. By contrast, we show that the remaining degree entropy and robustness have a positive monotonic relationship and give an analytic expression for the remaining degree entropy of the log-normal distribution. We also show that degree-degree correlations are not by themselves indicative of a network's robustness for real networks. We propose an adjustment to how mutual information is measured which better encapsulates structural properties related to robustness.
The dismembered bible
(2021)
It is often presumed that biblical redaction was invariably done using conventional scribal methods, meaning that when editors sought to modify or compile existing texts, they would do so in the process of rewriting them upon new scrolls. There is, however, substantial evidence pointing to an alternative scenario: Various sections of the Hebrew Bible appear to have been created through a process of material redaction. In some cases, ancient editors simply appended new sheets to existing scrolls. Other times, they literally cut and pasted their sources, carving out patches of text from multiple manuscripts and then gluing them together like a collage. Idan Dershowitz shows how this surprising technique left behind telltale traces in the biblical text - especially when the editors made mistakes - allowing us to reconstruct their modus operandi. Material evidence from the ancient Near East and elsewhere further supports his hypothesis.
With the present study, we introduce a fast and robust method to calculate the source displacement spectra of small earthquakes on a local to regional scale. The work is based on the publicly available Qopen method of full envelope inversion, which is further tuned for the given purpose. Important source parameters-seismic moment, moment magnitude, corner frequency, and high-frequency fall off-are determined from the source spectra by fitting a simple earthquake source model. The method is demonstrated by means of a data set comprising the 2018 West Bohemia earthquake swarm. We report moment magnitudes, corner frequencies, and centroid moment tensors inverted from short-period body waves with the Grond package for all earthquakes with a local magnitude larger than 1.8. Moment magnitudes calculated by envelope inversion show a very good agreement to moment magnitudes resulting from the probabilisitc moment tensor inversion. Furthermore, source displacement spectra from envelope inversion show a good agreement with spectra obtained by multiple taper analysis of the direct onsets of body waves but are not affected by the large scatter of the second. The seismic moments obtained with the envelope inversion scale with corner frequencies according to M-0 proportional to f(c)(-4.7). Earthquakes of the present data set result in a smaller stress drop for smaller magnitudes. Self-similarity of earthquake rupture is not observed. In addition, we report frequency-dependent site amplification at the used stations.
We discuss the coherent splitting and recombining of a nanoparticle in a mesoscopic "closed-loop" Stern-Gerlach interferometer in which the observable is the spin of a single impurity embedded in the particle.
This spin, when interacting with a pulsed magnetic gradient, generates the force on the particle.
We calculate the internal decoherence, which arises as the displaced impurity excites internal degrees of freedom (phonons) that may provide WelcherWeg information and preclude interference.
We estimate the constraints this decoherence channel puts on future interference experiments with massive objects. We find that for a wide range of masses, forces, and temperatures, phonons do not inhibit Stern-Gerlach interferometry with micro-scale objects.
However, phonons do constitute a fundamental limit on the splitting of larger macroscopic objects if the applied force induces phonons.
The radiation model is a parameter-free model of human mobility that has been applied primarily for short-distance moves, such as commuting. When applied to migration, it underestimates the number of long-range moves, such as between different US states. Here we show that it additionally suffers from a conceptual inconsistency that can have substantial numerical effects on long-distance moves.
We propose a modification of the radiation model that introduces a dependence on the angle between any two alternative potential destinations, accounting for the possibility that migrants may have preferences about the approximate direction of their move.
We demonstrate that this modification mitigates the conceptual inconsistency and improves the model fit to observational migration data, without introducing any fitting parameters.
The Shanderman lamprophyre dykes crop out in the western part of the Alborz Mountains (Talesh).
These rocks are classified as camptonites, composed of primary olivine, Ti-rich diopside, kaersutite, biotite, plagioclase, K-feldspar, and minor Ti-rich spinels, magnetite, pentlandite-pyrrhotite/chalcopyrite, and powellite-scheelite. Secondary analcime-wairakite, serpentines, and prehnite are common minor minerals within the studied rocks.
Olivine, Ti-rich diopside, spinel, and amphibole show distinct chemical zoning. Spinels display a core-to-rim decrease in Cr2O3, MgO, and Al2O3 concentrations and an increase in TiO2 and FeOT (total Fe as FeO), reflecting the oxidation state increase due to hydrothermal fluid influx. Low SiO2 contents (< 42 wt%), high MgO (12.44 to 13.98 wt%), and Fe2O3T (12.76 to 13.43 wt%), Cr (318-537 mu g/g) and Ni (231-327 mu g/g) contents indicate the ultrabasic nature of the rocks.
The samples show potassic character (2.1-2.8 wt% K2O), along with elevated LREE and LILE, and also exhibit minor positive Eu anomalies (Eu/Eu* = 1.09 to 1.20).
Olivine-spinel geothermometry indicates a maximum crystallization temperature of 1227 degrees C (ave. 988 degrees C +/- 65 degrees C).
Exsolution of pentlandite-pyrrhotite/chalcopyrite solid solutions occurred during magma cooling and crystallization. At lower temperatures, analcime-wairakite and prehnite partially replaced plagioclases.
The geochemical modeling of the rocks indicates the Shanderman lamprophyre magmas were derived from low-grade melting (< 5%) of amphibole-bearing garnet lherzolite source without or with very few phlogopites.
The primary magma of Shanderman lamprophyres was derived from a depth of similar to 135 km by partial melting of a metasomatized mantle source in a post-collisional environment.
Contour scanning and process gas type are process parameters typically considered achieving second order effects compared to first order factors such as laser power and scanning speed.
The present work highlights that contour scanning is crucial to ensure geometrical accuracy and thereby the high performance under uniaxial compression of complex Alloy 718 lattice structures.
Studies of X-ray computed tomography visualizations of as-built and compression-strained structures reveal the continuous and smooth bending and compression of the walls, and the earlier onset of internal contact appearance in the denser lattices printed with contour. In contrast, the effect of addition of He to the Ar process gas appears to have limited influence on the mechanical response of the lattices and their microstructure as characterized by electron backscattered diffraction.
However, the addition of He proved to significantly enhance the cooling rate and to reduce the amount of the generated spatters as evidenced by in situ monitoring of the process emissions, which is very promising for the process stability and powder reusability during laser powder bed fusion.
In this work, we investigate the photo-aquation reaction of the ferrocyanide anion with multi-edge picosecond soft X-ray spectroscopy.
Combining the information of the iron L-edge with nitrogen and oxygen K-edges, we carry out a complete characterization of the bonding channels in the [Fe(CN)(5)(H2O)](3-) photo-product.
We observe clear spectral signatures of covalent bonding between water and the metal, reflecting the mixing of the Fe d(z)(2) orbital with the 3a(1) and 4a(1) orbitals of H2O. Additional fingerprints related to the symmetry reduction and the resulting loss in orbital degeneracy are also reported.
The implications of the elucidated fingerprints in the context of future ultra-fast experiments are also discussed.
Ground subsidence caused by natural or anthropogenic processes affects major urban areas worldwide. Sinkhole formation and infrastructure fractures have intensified in the federal capital of Maceio (Alagoas, Brazil) since early 2018, forcing authorities to relocate affected residents and place buildings under demolition. In this study, we present a 16-year history (2004-2020) of surface displacement, which shows precursory deformations in 2004-2005, reaching a maximum cumulative subsidence of approximately 200 cm near the Mundau Lagoon coast in November 2020. By integrating the displacement observations with numerical source modelling, we suggest that extensive subsidence can be primarily associated with the removal of localized, deep-seated material at the location and depth where salt is mined. We discuss the accelerating subsidence rates, influence of severe precipitation events on the aforementioned geological instability, and related hazards. This study suggests that feedback destabilization mechanisms may arise in evaporite systems due to anthropogenic activities, fostering enhanced and complex superficial ground deformation.
To plan cluster-randomized trials with sufficient statistical power to detect intervention effects on student achievement, researchers need multilevel design parameters, including measures of between-classroom and between-school differences and the amounts of variance explained by covariates at the student, classroom, and school level. Previous research has mostly been conducted in the United States, focused on two-level designs, and limited to core achievement domains (i.e., mathematics, science, reading). Using representative data of students attending grades 1-12 from three German longitudinal large-scale assessments (3,963 <= N <= 14,640), we used three- and two-level latent (covariate) models to provide design parameters and corresponding standard errors for a broad array of domain-specific (e.g., mathematics, science, verbal skills) and domain-general (e.g., basic cognitive functions) achievement outcomes. Three covariate sets were applied comprising (a) pretest scores, (b) sociodemographic characteristics, and (c) their combination. Design parameters varied considerably as a function of the hierarchical level, achievement outcome, and grade level. Our findings demonstrate the need to strive for an optimal fit between design parameters and target research context. We illustrate the application of design parameters in power analyses.
In the Universe, matter outside of stars and compact objects is mostly composed of collisionless plasma.
The interaction of a supersonic plasma flow with an obstacle results in collisionless shocks that are often associated with intense nonthermal radiation and the production of cosmic ray particles.
Motivated by simulations of non-relativistic high-Mach-number shocks in supernova remnants, we investigate the instabilities excited by relativistic electron beams in the extended foreshock of oblique shocks.
The phase-space distributions in the inner and outer foreshock regions are derived with a particle-in-cell simulation of the shock and used as initial conditions for simulations with periodic boundary conditions to study their relaxation toward equilibrium.
We find that the observed electron-beam instabilities agree very well with the predictions of a linear dispersion analysis: the electrostatic electron-acoustic instability dominates in the outer region of the foreshock, while the denser electron beams in the inner foreshock drive the gyroresonant oblique-whistler instability.
The purpose of this study was to examine the moderating effects of technology use for relationship maintenance on the longitudinal associations among self-isolation during the coronavirus-19 (COVID-19) pandemic and romantic relationship quality among adolescents. Participants were 239 (120 female; M age = 16.69, standard deviation [SD] = 0.61; 60 percent Caucasian) 11th and 12th graders from three midwestern high schools. To qualify for this study, adolescents had to be in the same romantic relationship for the duration of the study, similar to 7 months (M length of relationship = 10.03 months). Data were collected in October of 2019 (Time 1) and again 7 months later in May of 2020 (Time 2). Adolescents completed a romantic relationship questionnaire at Time 1 and again at Time 2, along with questionnaires on frequency of self-isolation during the COVID-19 pandemic and use of technology for romantic relationship maintenance. Findings revealed that increases in self-isolation during the COVID-19 pandemic related positively to the use of technology for romantic relationship maintenance and negatively to Time 2 romantic relationship quality. High use of technology for romantic relationship maintenance buffered against the negative effects of self-isolation during the COVID-19 pandemic on adolescents' romantic relationship quality 7 months later, whereas low use strengthened the negative relationship between self-isolation during the COVID-19 pandemic and romantic relationship quality. These findings suggest the importance of considering the implications of societal crisis or pandemics on adolescents' close relationships, particularly their romantic relationships.
Geochemical homogeneity in shale is often assumed when tracing subsurface fluids and characterizing sedimentary basins. This study presents measurements of the bulk gas composition, stable isotopes, and noble gas volume fraction and isotopes for shale gas samples collected from gas wells in the Wufeng-Longmaxi Shale, the southern Sichuan Basin, China. The dryness [C-1 /(C-2 + C-3)] ranging from 166.3 to 251.2, combined with delta C-13(1) and delta DC1 that vary from -28.8 to -27.3 parts per thousand and - 153 to -145 parts per thousand, respectively, point to a late mature thermogenic origin of hydrocarbon gas. He-3/He-4 ratios of gas samples are around 0.01 times the air value suggesting dominantly crust-derived He. Ne-21/Ne-22 and Ar-40/Ar-36 ratios of many gas samples are higher than the corresponding air values indicating the mixing of crustal and atmospheric noble gases. Multiple dichotomous patterns are observed in noble gas signatures of forelimb and backlimb samples, and depression and crest samples. Ne-20/Ne-22 ratios of some crest samples are higher than that of depression samples in the backlimb, pointing to the presence of diffusion-driven fractionation that is likely caused by the long-distance migration from depression to crest. Elemental ratios of air-derived noble gas isotopes - Ne-22/Ar-36, Kr-84/Ar-36, and Xe-132/Ar-36 are compared to the recharge water values, suggesting the interactions of oil, gas, and water phases in the shale over geologic time. Forelimb samples generally display older ages than backlimb samples, indicating a larger flux of external radiogenic He-4 due to the higher density of deep faults in the forelimb area caused by the basementinvolved deformation. The basement-involved deformation also causes pore collapse especially in the forelimb leading to a lower porosity that results in a more pristine noble gas signature in the forelimb due to the reduced impact of younger recharge water.
The present study examined the moderating role of parents' victimization status during adolescence in the associations between parenting styles, depression, and anxiety among relationally victimized adolescents. There were 436 relationally victimized adolescents (M age = 13.26 years, SD = .56) included in this study, along with their parents (n = 436; M age = 47.01 years, SD = .83; 86% mothers), from the Midwestern region of the United States (N = 872). Adolescents completed questionnaires on their experiences of relational victimization, depression, and anxiety, and perceptions of their parents' parenting styles. Their parents completed a questionnaire on their relational victimization status during adolescence. Findings from the study revealed that the relationships between the permissive parenting style, depression, and anxiety among relationally victimized adolescents were stronger when adolescents' parents were also victims of relational bullying during adolescence. These findings underscore the importance of considering parents' peer victimization history during adolescence.
Understanding the assembly mechanism and function of membrane proteins is a fundamental problem in biochemical research. Among the membrane proteins, G protein-coupled receptors (GPCRs) represent the largest class in the human body and have long been considered to function as monomers.
Nowadays, the oligomeric assembly of GPCRs is widely accepted, although the functional importance and therapeutic intervention remain largely unexplored. This is partly due to difficulties in the heterologous production of membrane proteins.
Cell-free protein synthesis (CFPS) with its endogenous endoplasmic reticulum-derived structures has proven as a technique to address this issue.
In this study, we investigate for the first time the conceptual CFPS of a heteromeric GPCR, the gamma-aminobutyric acid receptor type B (GABA(B)), from its protomers BR1 and BR2 using a eukaryotic cell-free lysate. Using a fluorescence-based proximity ligation assay, we provide evidence for colocalization and thus suggesting heterodimerization.
We prove the heterodimeric assembly by a bioluminescence resonance energy transfer saturation assay providing the manufacturability of a heterodimeric GPCR by CFPS. Additionally, we show the binding of a fluorescent orthosteric antagonist, demonstrating the feasibility of combining the CFPS of GPCRs with pharmacological applications.
These results provide a simple and powerful experimental platform for the synthesis of heteromeric GPCRs and open new perspectives for the modelling of protein-protein interactions.
Accordingly, the presented technology enables the targeting of protein assemblies as a new interface for pharmacological intervention in disease-relevant dimers.
Analysis of single event transient effects in standard delay cells based on decoupling capacitors
(2022)
Single Event Transients (SETs), i.e., voltage glitches induced in combinational logic as a result of the passage of energetic particles, represent an increasingly critical reliability threat for modern complementary metal oxide semiconductor (CMOS) integrated circuits (ICs) employed in space missions.
In rad-hard ICs implemented with standard digital cells, special design techniques should be applied to reduce the Soft Error Rate (SER) due to SETs.
To this end, it is essential to consider the SET robustness of individual standard cells. Among the wide range of logic cells available in standard cell libraries, the standard delay cells (SDCs) implemented with the skew-sized inverters are exceptionally vulnerable to SETs. Namely, the SET pulses induced in these cells may be hundreds of picoseconds longer than those in other standard cells.
In this work, an alternative design of a SDC based on two inverters and two decoupling capacitors is introduced. Electrical simulations have shown that the propagation delay and SET robustness of the proposed delay cell are strongly influenced by the transistor sizes and supply voltage, while the impact of temperature is moderate. The proposed design is more tolerant to SETs than the SDCs with skew-sized inverters, and occupies less area compared to the hardening configurations based on partial and complete duplication.
Due to the low transistor count (only six transistors), the proposed delay cell could also be used as a SET filter.
Enhanced protective performance of waterborne, microcontainers-doped coatings in harsh environments
(2021)
In this study, the corrosion inhibitors Zinc oleate and 8-Hydroxyquinoline were successfully encapsulated using an interfacial polyaddition method. As such they were dispersed at different concentrations within the waterborne coating matrix. The resulting composite coatings were applied to the low carbon steel substrates. Successful synthesis and morphological characteristics of microcontainers loaded with inhibitors were confirmed using various characterization techniques. Scanning electron microscopy, dynamic light scattering, and thermogravimetric measurements are techniques used to define the surface, dimensional, and dispersive characteristics of containers, and the share of encapsulated inhibitors. The release study defined the discharge kinetics of the corrosion inhibitor from the microcontainers dispersed freely in an aqueous medium. Electrochemical impedance spectroscopy was used to determine the anticorrosive performance of the samples continuously exposed to various corrosive environments of salt and humidity chambers and NaCl solution. Special emphasis was placed on adhesion testing and visual observations during the exposure period. Significant improvements have been noted in terms of corrosion resistance, which, however, depend on the type of inhibitor used, the concentration of the containers embedded in the coating matrix and on the characteristics of the corrosive environment.
Turkish 3rd person plural subjects are frequently used with verbs that are unmarked for number, with plural suffix omission influenced by semantic and word-order related constraints.
Previous findings from judgment tasks indicate that monolingual and heritage Turkish speakers differ in the way they are affected by these constraints.
This study builds and expands upon previous research by investigating the role of word order in more detail, and by examining whether the constraint weightings obtained from Uygun and Felser's (2021) acceptability judgment data are able to predict speakers' verb form choices in a timed sentence completion task.
Besides confirming that word-order related constraints are information-structural in nature, our results show that heritage speakers over-produce plural-marked verbs in comparison to monolingual speakers, indicating between-group differences in constraint ranking.
We interpret this as reflecting a tendency among Turkish heritage speakers to regularize the agreement system, which is not necessarily observed in metalinguistic judgment tasks.
In the area of cardiac monitoring, the use of digitally driven technologies is on the rise. While the development of medical products is advancing rapidly, allowing for new use-cases in cardiac monitoring and other areas, regulatory and legal requirements that govern market access are often evolving slowly, sometimes creating market barriers. This article gives a brief overview of the existing clinical studies regarding the use of smart wearables in cardiac monitoring and provides insight into the main regulatory and legal aspects that need to be considered when such products are intended to be used in a health care setting. Based on this brief overview, the article elaborates on the specific requirements in the main areas of authorization/certification and reimbursement/compensation, as well as data protection and data security. Three case studies are presented as examples of specific market access procedures: the USA, Germany, and Belgium. This article concludes that, despite the differences in specific requirements, market access pathways in most countries are characterized by a number of similarities, which should be considered early on in product development. The article also elaborates on how regulatory and legal requirements are currently being adapted for digitally driven wearables and proposes an ongoing evolution of these requirements to facilitate market access for beneficial medical technology in the future.
Ground-based solar observations are severely affected by Earth's turbulent atmosphere. As a consequence, observed image quality and prevailing seeing conditions are closely related. Partial correction of image degradation is nowadays provided in real time by adaptive optics (AO) systems. In this study, different metrics of image quality are compared with parameters characterizing the prevailing seeing conditions, i.e. Median Filter Gradient Similarity (MFGS), Median Filter Laplacian Similarity (MFLS), Helmli-Scherer mean, granular rms-contrast, differential image motion, and Fried-parameter r(0). The quiet-Sun observations at disk center were carried out at the Vacuum Tower Telescope (VTT), Observatorio del Teide (OT), Izana, Tenerife, Spain. In July and August 2016, time series of short-exposure images were recorded with the High-resolution Fast Imager (HiFI) at various wavelengths in the visible and near-infrared parts of the spectrum. Correlation analysis yields the wavelength dependence of the image quality metrics and seeing parameters, and Uniform Manifold Approximation and Projection (UMAP) is employed to characterize the seeing on a particular observing day. In addition, the image quality metrics and seeing parameters are used to determine the field dependence of the correction provided by the AO system. Management of high-resolution imaging data from large-aperture, ground-based telescopes demands reliable image quality metrics and meaningful characterization of prevailing seeing conditions and AO performance. The present study offers guidance on how retrieving such information ex post facto.
In this paper, we take a cognitive-sociolinguistic perspective on texts from the colonial period. The texts stem from various agents in the colonial enterprise and include documents from missionaries, administrators and politicians, as well as legal and scientific texts. What we find and trace in these texts is a recurrent set of dominant systems of conceptualizations that are characteristic of the colonial mindset and the corresponding discourse at large. However, these conceptualizations were spelled out in quite different ways in discourse, depending on the ideological background and objectives of the authors and on the specific colonial setting they deal with. We will focus on two contexts, India and sub-Saharan Africa, and we will highlight conceptualizations related to the framing of the constellation between colonizers and colonial subjects in terms of, inter alia, a parent-child, an adult-child and a teacher-pupil relationship. We will then look into some examples of cultural practices among the colonized that were “disturbing” to the colonizers. The fact that they were betrays value systems as well as preoccupations and fears on the side of the colonizers. These practices triggered efforts at cultural engineering in the colonies which had lasting effects on the local culture in these settings. However, this impact was far from being one-directional. The experience with the “otherness” of the colonial subjects fueled debates on latent societal issues in the culture of the colonizers. We will consider this impact for the case of the discourse on homosexuality. The empire stroke back also in linguistic terms, most notably by a host of loan words that entered the lexicon of English. The way these loan words were “integrated” into the English language provides ample evidence of a cultural appropriation also in this direction, i.e., the process known as “contextualization” in traditional Kachruvian sociolinguistics is bi-directional as well.
Theoretical and empirical studies show increased diversity in crops, supply chains, and markets helps stabilize food systems. At the same time global commodity markets and industrial agriculture have driven homogenization of local and regional production systems, and consolidated power in fewer larger specialized farms and distributers. This is a global challenge, with no obvious global solutions. An important question therefore, is how individual countries can build their own resilience through maintaining or increasing diversity within their borders. Here we show, using farm level data from Germany, that spreading production risk by growing the same crops across different farms carries stabilizing benefits by allowing for increased spatiotemporal asynchrony within crops. We also find that increasing asynchrony between the year-to-year production of different crops has stabilizing effects on food supply. Importantly, the benefits of increasing crop diversity are lower in specialized landscapes growing the same crop on large patches. Our results illustrate clear benefits of diversified crops, producers, and agricultural landscapes to buffer supply side shocks, and for incorporation in subsidies and other regulatory measures aimed at stabilizing food systems.
This chapter presents an overview of Cognitive Sociolinguistic studies of African English. We discuss early applications of Conceptual Metaphor Theory to the study of English in Cameroon (Wolf 1999, 2001; Wolf and Simo Bobda 2001) as well as the extensive and methodologically diverse body of Cognitive Sociolinguistic research on the cultural model of COMMUNITY expressed in West and East African English (e.g., Wolf 2006, 2008; Wolf and Polzenhagen 2007; Polzenhagen and Wolf 2007; Polzenhagen 2007). Moreover, the chapter illustrates how studies such as Finzel and Wolf (2017), Peters (2021), Finzel (forthcoming) and Peters and Polzenhagen (2021) extend the Cognitive Sociolinguistic approach to further sociocultural issues, such as gender identities and culture-specific strategies of advertising in different anglophone parts of Africa. Finally, we point out possible future applications of the paradigm to socio-pragmatic aspects of African English.
Each completely regular semigroup is a semilattice of completely simple semigroups. The more specific concept of a strong semilattice provides the concrete product between two arbitrary elements.
We characterize strong semilattices of rectangular groups by so-called disjunctions of identities. Disjunctions of identities generalize the classical concept of an identity and of a variety, respectively.
The rectangular groups will be on the one hand left zero semigroups and right zero semigroups and on the other hand groups of exponent p is an element of P, where P is any set of pairwise coprime natural numbers.
This chapter compares East and West African English as two distinct regional varieties of African English. First, the historical development of English in these two regions is briefly considered. It is argued that British colonial policy contributed significantly to the sociolinguistic and, indirectly, even structural differences these varieties exhibit. Then, the discussion moves on to give a short overview of the national sub-varieties. It is found that, although united by common linguistic features, West African English is far more heterogeneous than East African English, and some explanation is provided for this phenomenon. Focusing specifically on phonetic features, the chapter summarizes and contrasts the main diagnostic and distinctive features of each regional variety, with special reference to the peculiarities of the national varieties of West African English. However, despite their structural differences, West African, East African English and, for that matter, Southern African English are rooted in a shared “African culture.” Recent findings are introduced, in which common conceptual and linguistic patterns pertaining to witchcraft, expressed in the regional varieties in question, are highlighted.
Naomi
(2022)
Naomi is one of the dramatis personae of the book of Ruth, which is set by its author “in the days that the judges judged” (Ruth 1:1). She is introduced as wife of Elimelech and mother of Mahlon and Chilion, all of whom journeyed to Moab from Bethlehem, due to famine. By the fifth verse in the book’s first chapter, her husband and sons are dead, leaving Naomi with only her Moabite daughters-in-law, Orpah and Ruth (Ruth 1:1–5). Shortly thereafter, Orpah too exits the stage (Ruth 1:14). Against Naomi’s protestations, Ruth ties her own fate to her mother-in-law’s, joining Naomi on her journey back to Bethlehem (Ruth 1:4b, 8–19a).
Mordecai (Son of Jair)
(2021)
Mordecai, son of Jair, is the Deuteragonist of the book of Esther. Some time after the death of the eponymous Esther’s parents, Mordecai – Esther’s cousin – becomes her guardian (Esth 2:7). The MT version of the book also has Mordecai adopting Esther as his daughter (Esth 2:7, 15). In LXX, on the other hand, Mordecai takes her as his wife (Esth 2:7; cf. bMeg 13a). Although the syntax of Esth 2:5–6 is convoluted, it appears Mordecai is a Benjaminite deportee from Jerusalem – exiled by Nebuchadnezzar, along with King Jeconiah and others. Presuming a coherent historical and chronological framework, this would make Mordecai 114 years old at the time of the events, at the very least, which does not accord with his depiction throughout the novella. (This biographical detail is absent in the Greek Alpha Text of Esther.)
Jonah
(2023)
In the Masoretic canon of the Tanakh the book of Jonah appears as the fifth part of Tre Assar, or Twelve Minor Prophets, between Obadiah and Micah. In the Septuagint, on the other hand, Jonah appears as the sixth book in the series, and is followed immediately by Nahum. As both Jonah and Nahum speak out against the city of Nineveh, their chronology became an issue early in their discourses of reception (Liv. Pro. 11:1; Josephus, Ant. 9:239–242; Tg.Nah 1:1).
Cain and Abel
(2021)
The biblical story of Cain and Abel in Genesis 4:1–16 appears as the first case of siblings’ rivalry in the Torah. It is the starting point of a socio-ethical process of human development within the book of Genesis. The sibling narrative also includes the first report of homicide, more precisely a fratricide, as Cain slays his own brother Abel (Gen 4:8). The Jewish and Christian reception discourse of the Cain-Abel-story developed early on to deal with a range of open questions and difficult passages provided by the biblical text. The basic assumptions of Jewish and Christian interpretations are initially similar in terms of attempting to explain God’s preference for Abel’s sacrifice and Cain’s motivation for killing his brother.
A significant fraction of massive stars move at speed through the interstellar medium of galaxies.
After their death as core-collapse supernovae, a possible final evolutionary state is that of a fast-rotating magnetized neutron star, shaping its circumstellar medium into a pulsar wind nebula.
Understanding the properties of pulsar wind nebulae requires knowledge of the evolutionary history of their massive progenitors.
Using two-dimensional magnetohydrodynamical simulations, we demonstrate that, in the context of a runaway high-mass red-supergiant supernova progenitor, the morphology of its subsequent pulsar wind nebula is strongly affected by the wind of the defunct progenitor star pre-shaping the stellar surroundings throughout its entire past life.
In particular, pulsar wind nebulae of obscured runaway massive stars harbour asymmetries as a function of the morphology of the progenitor's wind-blown cavity, inducing projected asymmetric up-down synchrotron emission.
Religion und Geschlecht sind zentrale Wissenskategorien für die Entwicklung und Ausprägung jüdischer Identität in ihrer Selbst- und Fremdwahrnehmung. Gemäß Pierre Bourdieu lassen sich entsprechende Prozesse der Identitätsbildung als Habitualisierung verstehen, an welcher auch Jugendliteratur einen Anteil haben kann. Der Artikel legt anhand der Jugendromane Bella und das Mädchen aus dem Schtetl (2015) und Dunkles Gold (2019) exemplarisch dar, mit welchen Methoden und Fragestellungen Geschlechtlichkeit und geschlechtlich markierte Sozialisationsangebote innerhalb religionsaffiner Kontexte jüdischer Jugendliteratur untersucht werden können.
Equilibrium mass-dependent ("stable") isotopic fractionation of an element during magmatic processes is driven by a contrast in bonding environment between minerals and silicate melt, which is expressed as an isotopic fractionation factor.
A quantitative understanding of such isotopic fractionation factors is vital to interpret observed isotopic variations in magmatic rocks.
It is well known that the local environment and the bond strength of an element dictate the sign and magnitude of isotopic fractionation between minerals, but it is uncertain how the structure and chemical composition of a silicate melt can affect mineral-melt isotopic fractionation factors.
To explore this, we studied the coordination environment of nickel (Ni) in different silicate glasses using extended X-ray absorption fine structure (EXAFS) measurements at the German synchrotron X-ray source (DESY).
We determined -Ni-O bond lengths in a suite of synthetic but near-natural silicate glasses using EXAFS and found that the former vary systematically with melt alkalinity, which is best described by the parameter ln[1 + (Na + K)/Ca]. With increasing melt alkalinity, Ni occupies more IV-fold coordinated sites, which are associated with a shorter -Ni-O bond length. Next, we use the ionic model, which allows to predict isotopic fractionation factors based on the difference in bond length between two phases.
We find that more alkaline melts have a stronger preference for the heavier isotopes of Ni than less alkaline melts. This implies that the magnitude of mineral-melt Ni isotope fractionation factors, for instance between olivine and melt, will depend on the alkalinity of the melt.
At magmatic temperatures, however, the variation in fractionation factors caused by melt alkalinity will rarely exceed 0.05 parts per thousand and is thus mostly negligible, in particular in the realm of basaltic melt compositions. Nevertheless, the relationship between melt alkalinity and fractionation factor reported here can be used to extrapolate empirical data for mineral-melt Ni isotope fractionation factors, once such data become available, to the full range of magma compositions on Earth and other Solar System bodies.
The body as a wonderland
(2024)
Reentrant tensegrity
(2021)
We present a three-periodic, chiral, tensegrity structure and demonstrate that it is auxetic. Our tensegrity structure is constructed using the chiral symmetry Pi(+) cylinder packing, transforming cylinders to elastic elements and cylinder contacts to incompressible rods. The resulting structure displays local reentrant geometry at its vertices and is shown to be auxetic when modeled as an equilibrium configuration of spatial constraints subject to a quasi-static deformation. When the structure is subsequently modeled as a lattice material with elastic elements, the auxetic behavior is again confirmed through finite element modeling. The cubic symmetry of the original structure means that the auxetic behavior is observed in both perpendicular directions and is close to isotropic in magnitude. This structure could be the simplest three-dimensional analog to the two-dimensional reentrant honeycomb. This, alongside the chirality of the structure, makes it an interesting design target for multifunctional materials.
Landslide hazard models aim at mitigating landslide impact by providing probabilistic forecasting, and the accuracy of these models hinges on landslide databases for model training and testing.
Landslide databases at times lack information on the underlying triggering mechanism, making these inventories almost unusable in hazard models.
We developed a Python-based unique library, Landsifier, that contains three different machine-Learning frameworks for assessing the likely triggering mechanisms of individual landslides or entire inventories based on landslide geometry.
Two of these methods only use the 2D landslide planforms, and the third utilizes the 3D shape of landslides relying on an underlying digital elevation model (DEM). The base method extracts geometric properties of landslide polygons as a feature space for the shallow learner - random forest (RF).
An alternative method relies on landslide planform images as an input for the deep learning algorithm - convolutional neural network (CNN).
The last framework extracts topological properties of 3D landslides through topological data analysis (TDA) and then feeds these properties as a feature space to the random forest classifier.
We tested all three interchangeable methods on several inventories with known triggers spread over the Japanese archipelago. To demonstrate the effectiveness of developed methods, we used two testing configurations.
The first configuration merges all the available data for the k-fold cross-validation, whereas the second configuration excludes one inventory during the training phase to use as the sole testing inventory.
Our geometric-feature-based method performs satisfactorily, with classification accuracies varying between 67 % and 92 %. We have introduced a more straightforward but data-intensive CNN alternative, as it inputs only landslide images without manual feature selection.
CNN eases the scripting process without losing classification accuracy. Using topological features from 3D landslides (extracted through TDA) in the RF classifier improves classification accuracy by 12 % on average.
TDA also requires less training data. However, the landscape autocorrelation could easily bias TDA-based classification. Finally, we implemented the three methods on an inventory without any triggering information to showcase a real-world application.
Riverine ecosystems provide various ecosystem services. One of these services is the biological control of eutrophication by grazing macroinvertebrates.
However, riverine ecosystems are subject to numerous stressors that affect community structure, functions, and stability properties. To manage rivers in response to these stressors, a better understanding of the ecological functions underlying services is needed.
This requires consideration of local and regional processes, which requires a metacommunity approach that links local food webs through drift and dispersal. This takes into account long-distance interactions that can compensate for local effects of stressors.
Our modular model MASTIFF (Multiple Aquatic STressors In Flowing Food webs) is stage-structured, spatially explicit, and includes coupled food webs consisting of benthic resource-consumer interactions between biofilm and three competing macroinvertebrate functional types. River segments are unidirectionally connected through organismal drift and bidirectionally connected through dispersal. Climate and land use stressors along the river can be accounted for. Biocontrol of biofilm eutrophication is used as an exemplary functional indicator.
We present the model and the underlying considerations, and show in an exemplary application that explicit consideration of drift and dispersal is essential for understanding the spatiotemporal biocontrol of eutrophication.
The combination of drift and dispersal reduced eutrophication events. While dispersal events were linked to specific periods in the species life cycles and therefore had limited potential to control, drift was ubiquitous and thus responded more readily to changing habitat conditions.
This indicates that drift is an important factor for coping with stress situations.
Finally, we outline and discuss the potential and possibilities of MASTIFF as a tool for mechanistic, cross-scale analyses of multiple stressors to advance knowledge of riverine ecosystem functioning.
Self-organized patterns in the actin cytoskeleton are essential for eukaryotic cellular life. They are the building blocks of many functional structures that often operate simultaneously to facilitate, for example, nutrient uptake and movement of cells. However, identifying how qualitatively distinct actin patterns can coexist remains a challenge.
Using bifurcation theory of a mass conserved activator-inhibitor system, we uncover a generic mechanism of how different actin waves-traveling waves and excitable pulses- organize and simultaneously emerge.
Live-cell imaging experiments indeed reveal that narrow, planar, and fast-moving excitable pulses may coexist with ring-shaped macropinocytic actin waves in the cortex of motile amoeboid cells.
Over the past years, NGS has become a crucial workhorse for open-view pathogen diagnostics.
Yet, long turnaround times result from using massively parallel high-throughput technologies as the analysis can only be performed after sequencing has finished. The interpretation of results can further be challenged by contaminations, clinically irrelevant sequences, and the sheer amount and complexity of the data.
We implemented PathoLive, a real-time diagnostics pipeline for the detection of pathogens from clinical samples hours before sequencing has finished.
Based on real-time alignment with HiLive2, mappings are scored with respect to common contaminations, low-entropy areas, and sequences of widespread, non-pathogenic organisms.
The results are visualized using an interactive taxonomic tree that provides an easily interpretable overview of the relevance of hits. For a human plasma sample that was spiked in vitro with six pathogenic viruses, all agents were clearly detected after only 40 of 200 sequencing cycles.
For a real-world sample from Sudan, the results correctly indicated the presence of Crimean-Congo hemorrhagic fever virus. In a second real-world dataset from the 2019 SARS-CoV-2 outbreak in Wuhan, we found the presence of a SARS coronavirus as the most relevant hit without the novel virus reference genome being included in the database.
For all samples, clinically irrelevant hits were correctly de-emphasized.
Our approach is valuable to obtain fast and accurate NGS-based pathogen identifications and correctly prioritize and visualize them based on their clinical significance: PathoLive is open source and available on GitLab and BioConda.
This joint experimental-theoretical work focuses on molecular and photophysical properties of the spiropyran-containing amphiphilic molecule in organic and aqueous solutions.
Being dissolved in tested organic solvents, the system demonstrates positive photochromism, i.e., upon UV stimulus the colorless spiropyran form is transformed into colorful merocyanine isomer.
However, the aqueous solution of the amphiphile possesses a negative photochromism: the orange-red merocyanine form becomes thermodynamically more stable in water, and both UV and vis stimuli lead to the partial or complete photobleaching of the solution.
The explanation of this phenomenon is given on the basis of density functional theory calculations and classical modeling including thermodynamic integration.
The simulations reveal that stabilization of merocyanine in water proceeds with the energy of ca. 70 kJ mol-1, and that the Helmholtz free energy of hydration of merocyanine form is 100 kJ mol-1 lower as compared to the behavior of SP isomer in water.
The explanation of such a difference lies in the molecular properties of the merocyanine: after ring-opening reaction this molecule transforms into a zwitterionic form, as evidenced by the electrostatic potential plotted around the opened form.
The presence of three charged groups on the periphery of a flat conjugated backbone stimulates the self-assembly of merocyanine molecules in water, ending up with the formation of elongated associates with stack-like building blocks, as shown in molecular dynamics simulations of the aqueous solution with the concentration above critical micelle concentration.
Our quantitative evaluation of the hydrophilicity switching in spiropyran/merocyanine containing surfactants may prompt the search for new systems, including colloidal and polymeric ones, aiming at remote tuning of their morphology, which could give new promising shapes and patterns for the needs of modern nanotechnology.
Noble metal nanostructures are known to confine photon energies to their dimensions with resonant oscillations of their conduction electrons, leading to the ultrahigh enhancement of electromagnetic fields in numerous spectroscopic methods.
Of all the possible plasmonic nanomaterials, silver offers the most intriguing properties, such as best field enhancements and tunable resonances in visible-to-near infrared regions.
This review highlights the recent developments in silver nanostructured substrates for plasmonic sensing with the main emphasis on surface plasmon resonance (SPR) and surface-enhanced Raman spectroscopy (SERS) over the past decade.
The main focus is on the synthesis of silver nanostructured substrates via physical vapor deposition and chemical synthesis routes and their applications in each sensing regime.
A comprehensive review of recent literature on various possible silver nanostructures prepared through these methodologies is discussed and critically reviewed for various planar and optical fiber-based substrates.
Origin and migration of fluoride in the area of the Aluto Volcanic Complex (Main Ethiopian Rift)
(2022)
Fluoride-enriched ground and surface waters represent a major health risk for the local population in many areas along the East African Rift.
The present study investigates the origin of fluoride and the reason for its accumulation in the rift waters, following two hypotheses: (i) fluid-rock-interactions release fluoride from minerals into the water and (ii) magmatic-derived fluoride-containing liquids and gases migrate along permeable fault zones until they dissolve in ground-and surface water or be released to the atmosphere.
Rock-, gas, water-, soil-, and plant samples were collected from the area within and close by the Aluto Volcanic Complex, which is part of the Main Ethiopian Rift. Most analyzed waters showed fluoride concentrations above the drinking water limit (> 1.5 mg/L) with the highest values in hot springs (up to 70 mg/L) and the geothermal well (76 mg/L).
In the solid phase, a high fluoride content was found in acid volcanic rocks (ignimbrite: 4391 ppm; rhyolite: 3248 ppm) as well as in pumice (up to 1955 ppm). The fluoride content of soil samples collected within the volcanic complex varied between 82 and 1036 ppm, whereas former lake sediments from outside the Aluto Volcanic Complex showed higher fluoride contents ranging from 674 to 8171 ppm. Identified fluoride-rich minerals are various amphiboles (about 3 wt.-% F-), fluor-apatite (about 3 wt % F-), minerals of the fluorcaphite group (about 5 wt.-% F-), parisite (up to 9 wt.-% F-), and fluorite (CaF2).
Elevated fluoride concentrations were also measured in some gas samples from fumaroles (up to 50 ppm) and in plant samples collected next to the fumaroles (up to 65 ppm). Leaching experiments of solid samples with deionized water demonstrated that fluoride can easily be mobilized from pumice and sediments but hardly from consolidated rocks.
This fluoride release increased with temperature (up to 150 C) and correlated roughly with dis-solved silica indicating the binding of some fluoride to the amorphous or weakly crystalline silica fraction. Based on these results it was concluded that fluoride migrates via different pathways through the environment: At the depth of the magma chamber during magmatic differentiation fluoride enriches initially in the magmatic melt and accumulates in some late-crystallizing minerals of igneous rocks such as fluorite or mica. Upon volcanic eruption fluoride is predominantly incorporated in the glass (ignimbrite) and ash phase (pumice).
On the surface, these rocks are exposed to weathering and fluoride leaches partly out into the aqueous phase. Soft and porous rocks such as pumice release fluoride first whereas hard extrusive/igneous rocks are less prone to weathering and retain the fluoride. Pumice and (lake) sediments might act both, as source and as sink for fluoride in the area.
Although some fluoride might drain from the surface into the deeper subsoil, we conclude that magmatic fluids (liquid and gas) contribute more substantially to the overall fluoride ground water content because (i) of the much higher fluoride content in deep geothermal waters and hot springs as compared to surface-near waters; (ii) active geothermal surface manifestations located along fault zones indicate that fluids migrate from deep magmatic intrusions (as gas and liquid) towards the surface, where fluoride dissolves in groundwater; (iii) and the good correlation between bicarbonate (deriving from dissolution of magmatic CO2) and fluoride content in all analyzed water samples.
Carbon suboxide (C3O2) is a unique molecule able to polymerize spontaneously into highly conjugated light-absorbing structures at temperatures as low as 0 degrees C. Despite obvious advantages, little is known about the nature and the functional properties of this carbonaceous material. In this work, the aim is to bring "red carbon," a forgotten polymeric semiconductor, back to the community's attention.
A solution polymerization process is adapted to simplify the synthesis and control the structure.
This allows one to obtain this crystalline covalent material at low temperatures. Both spectroscopic and elemental analyses support the chemical structure represented as conjugated ladder polypyrone ribbons.
Density functional theory calculations suggest a crystalline structure of AB stacks of polypyrone ribbons and identify the material as a direct bandgap semiconductor with a medium bandgap that is further confirmed by optical analysis.
The material shows promising photocatalytic performance using blue light.
Moreover, the simple condensation-aromatization route described here allows the straightforward fabrication of conjugated ladder polymers and can be inspiring for the synthesis of carbonaceous materials at low temperatures in general.
Archive haben die Aufgabe, Wissen zu bewahren und zugänglich zu machen. Die Sammlungen des Museums für Naturkunde Berlin (MfN) wuchsen während der Zeit der europäischen Kolonialexpansion stark an. Naturalien aus der ganzen Welt gelangten nach Berlin und gleichzeitig fand ein wissenschaftlicher Austausch zu denselben statt. Die Spuren dieser Objekte und der Korrespondenzen können im Archiv des Museums nachverfolgt werden. Heute gelten koloniale Kontexte weitestgehend als Unrechtskontexte, deren Aufarbeitung gefordert wird. Um Provenienzforschung betreiben zu können, ist es daher unerlässlich, dass Museen und Archive ihre Sammlungen offenlegen (soweit rechtlich und ethisch möglich) und Außenstehenden den Zugriff ermöglichen.
Im Rahmen dieser Masterarbeit soll der respektvolle Umgang mit Archivgut aus kolonialen Kontexten kritisch reflektiert und Handlungsfelder für einen kulturell angemessenen Umgang mit sensiblen Inhalten aufgezeigt werden. Konkret beziehen sich die Handlungsoptionen auf Archivgut aus kolonialen Kontexten mit Bezug zu Australien. Dabei werden Provenienzforschung, Sensibilität, Mehrsprachigkeit, indigenes kulturelles Wissen (ICIP) sowie Plattform- und Schnittstellenoptionen für die Vernetzung von Daten und Inhalten bedacht. Ziel ist es, vor dem Hintergrund der Archive als Orte kulturellen Gedächtnisses den Umgang mit Archivgut aus kolonialen Kontexten zu reflektieren.
In this paper, we propose a revised fractional Brownian motion run with a nonlinear clock (fBm-nlc) model and utilize it to illustrate the microscopic mechanism analysis of the fractal derivative diffusion model with variable coefficient (VC-FDM).
The power-law mean squared displacement (MSD) links the fBm-nlc model and the VC-FDM via the two-parameter power law clock and the Hurst exponent is 0.5.
The MSD is verified by using the experimental points of the chloride ions diffusion in concrete.
When compared to the linear Brownian motion, the results show that the power law MSD of the fBm-nlc is much better in fitting the experimental points of chloride ions in concrete.
The fBm-nlc clearly interprets the VC-FDM and provides a microscopic strategy in characterizing different types of non-Fickian diffusion processes with more different nonlinear functions.
Paleogenomes reveal a complex evolutionary history of late Pleistocene bison in Northeastern China
(2022)
Steppe bison are a typical representative of the Mid-Late Pleistocene steppes of the northern hemisphere.
Despite the abundance of fossil remains, many questions related to their genetic diversity, population structure and dispersal route are still elusive.
Here, we present both near-complete and partial mitochondrial genomes, as well as a partial nuclear genome from fossil bison samples excavated from Late Pleistocene strata in northeastern China.
Maximum-likelihood and Bayesian trees both suggest the bison clade are divided into three maternal haplogroups (A, B and C), and Chinese individuals fall in two of them. Bayesian analysis shows that the split between haplogroup C and the ancestor of haplogroups A and B dates at 326 ky BP (95% HPD: 397-264 ky BP).
In addition, our nuclear phylogenomic tree also supports a basal position for the individual carrying haplogroup C. Admixture analyses suggest that CADG467 (haplogroup C) has a similar genetic structure to steppe bison from Siberia (haplogroup B).
Our new findings indicate that the genetic diversity of Pleistocene bison was probably even higher than previously thought and that northeastern Chinese populations of several mammalian species, including Pleistocene bison, were genetically distinct.
Forward stratigraphic modelling is a fast-developing modelling approach, used to test conceptual models, and predict stratigraphic architecture and depositional facies from basin to reservoir scales.
Published subsurface applications demonstrate its added value by integrating multidisciplinary data as well as geological concepts into its constraints.
When applied to carbonate depositional systems, composed of multiple sediment factories, the cooperating and interdependent production mechanisms remain poorly studied.
By applying the technique to a well-studied section of the Maldives carbonate platform, a specific model design-adapted to the geological age and setting, and constrained by available data-sheds light on the interaction of its carbonate producers.
The results yield a naturalistic depositional facies distribution and offer insight in the changing relationship between biotic communities during the platform evolution.
After calibration, the reference model unequivocally links the formerly proposed genetic model to the seismostratigraphic architecture.
Furthermore, the results show how environmental changes (seemingly of secondary impact compared to changes in physical accommodation in the stratigraphic record) can induce substantial fluctuations in carbonate production rates of biotic communities, affect the ecological accommodation, and thus impact the platform architecture. Therefore, it is crucial to treat carbonate production rates during periods of environmental change as variables with associated uncertainties in a forward stratigraphic model setup.
A special class of English words with tense vowel/diphthong nuclei and liquid codas receive variable syllable count judgments (one and over-one syllables).
Tilsen and Cohn (2016) showed that differences in judgments correlate with differences in production, supporting their hypothesis that meta-phonological judgments and speech motor control share a common representation.
In the present study, we further propose that syllable count judgments are related to subsegmental representation in the rime, and are independent of acoustic duration.
We test the hypothesis by comparing English and German, chosen for their similar word structures and vowel length contrast, and their crucial difference in the gestural specification of coda liquids.
In English, coda liquids have an earlier vocalic gesture relative to the consonantal one, while in German, both gestures are simultaneous.
We stipulated that sesquisyllabic (over-one) judgments are related to the count of sequentially-timed vocalic gestures in the rime. The difference in the coda liquid composition between the two languages predicts that sesquisyllables should not emerge in German.
Our predictions were confirmed by the results of parallel production (acoustic) and syllable count judgment experiments in English and German.
We propose a model accounting for these results, and we discuss its typological implications and its limitations.
Production and comprehension of prosodic boundary marking in persons with unilateral brain lesions
(2022)
Purpose: Persons with unilateral brain damage in the right hemisphere (RH) or left hemisphere (LH) show limitations in processing linguistic prosody, with yet inconclusive results on their ability to process prosodically marked structural boundaries for syntactic ambiguity resolution. We aimed at systematically investigating production and comprehension of three prosodic cues (f(0) range, final lengthening, and pause) at structural boundaries in coordinate sequences in participants with right hemisphere brain damage (RHDP) and participants with left hemisphere brain damage (LHDP). Method: Twenty RHDP and 15 LHDP participated in our study.
Comprehension experiment: Participants and a control group listened to coordinate name sequences with internal grouping by a prosodically marked structural boundary (grouped condition, e.g., "(Gabi und Leni) # und Nina") or without internal grouping (ungrouped condition, e.g., "Gabi und Leni und Nina") and had to identify the target condition. The strength and combinations of prosodic cues in the stimuli were manipulated.
Production experiment: Participants were asked to produce coordinate sequences in the two conditions (grouped, ungrouped) in two different tasks: a Reading Aloud and a Repetition experiment. Accuracy of participants' productions was subsequently assessed in a rating study and productions were analyzed with respect to use of prosodic cues.
Results: In the Comprehension experiment, RHDP and LHDP had overall lower identification accuracies than unimpaired control participants and LHDP were found to have particular problems with boundary identification when the pause cue was reduced. In production, LHDP and RHDP employed all three prosodic cues for boundary marking, but struggled to clearly mark prosodic boundaries in 28% of all productions. Both groups showed better performance in reading aloud than in repetition. LHDP relied more on using f(0) range and pause duration to prosodically mark structural boundaries, whereas RHDP employed final lengthening more vigorously than LHDP in reading aloud.
Conclusions: We conclude that processing of linguistic prosody is affected in RHDP and LHDP, but not completely impaired. Therefore, prosody can serve as a relevant communicative resource. However, it should also be considered as a target area for assessment and treatment in both groups.
The printability of artificial defects inside the additively manufactured laser powder bed fusion (LPBF) 316L stainless steel is investigated.
The printing parameters of the LPBF process are optimized to produce artificial defects with reproducible sizes at desired positions while minimizing redundant porosity. The smallest obtained artificial defect is 90 mu m in diameter.
The accuracy of the geometry of the printed defect depends on both the height and the diameter in the input model.
The effect of artificial defects on the very-high-cycle fatigue (VHCF) behavior of LPBF 316L stainless steel is also studied. The specimens printed with artificial defects in the center are tested under VHCF using an ultrasonic machine.
Crack initiation is accompanied by the formation of a fine granular area (FGA), typical of VHCF. Despite the presence of relatively large artificial defects, FGA formation is observed around accidental natural printing defects closer to the surface, which can still be considered as internal. The causes for this occurrence are discussed.
Plant cell walls are versatile materials that can adopt a wide range of mechanical properties through controlled deposition of cellulose fibrils. Wall integrity requires a sufficiently homogeneous fibril distribution to cope effectively with wall stresses. Additionally, specific conditions, such as the negative pressure in water transporting xylem vessels, may require more complex wall patterns, e.g., bands in protoxylem.
The orientation and patterning of cellulose fibrils are guided by dynamic cortical microtubules.
New microtubules are predominantly nucleated from parent microtubules causing positive feedback on local microtubule density with the potential to yield highly inhomogeneous patterns. Inhomogeneity indeed appears in all current cortical array simulations that include microtubule-based nucleation, suggesting that plant cells must possess an as-yet unknown balancing mechanism to prevent it.
Here, in a combined simulation and experimental approach, we show that a limited local recruitment of nucleation complexes to microtubules can counter the positive feedback, whereas local tubulin depletion cannot.
We observe that nucleation complexes preferentially appear at the plasma membrane near microtubules. By incorporating our experimental findings in stochastic simulations, we find that the spatial behavior of nucleation complexes delicately balances the positive feedback, such that differences in local microtubule dynamics-as in developing protoxylem-can quickly turn a homogeneous array into a banded one. Our results provide insight into how the plant cytoskeleton has evolved to meet diverse mechanical requirements and greatly increase the predictive power of computational cell biology studies.
Spacing repeated study phases across multiple sessions instead of studying and restudying the learning material in one session only is an effective strategy to promote lasting learning. However, most studies demonstrating the spacing effect were conducted in the laboratory, using simple verbal material. Learning in educational contexts differs regarding the complexity and coherence of the learning material and concerning the role of motivational and affective learner characteristics. Studies conducted in educational contexts suggest that the spacing effect is not as robust here. For example, acquiring mathematical skills or nonrepeated, consecutive information does not reliably benefit from spacing. After an overview of studies addressing the spacing effect in the laboratory and in educational contexts, we discuss various open questions that need to be addressed by future research before recommending spacing as a learning strategy to promote meaningful and lasting learning at schools and universities.
As society paves its way towards device miniaturization and precision medicine, microscale actuation and transport become increasingly prominent research fields with high impact in both technological and clinical contexts.
In order to accomplish movement of micron-sized objects towards specific target sites, active biohybrid transport systems, such as motile living cells that act as smart biochemically powered microcarriers, have been suggested as an alternative to synthetic microrobots.
Inspired by the motility of leukocytes, we propose the amoeboid crawling of eukaryotic cells as a promising mechanism for transport of micron-sized cargoes and present an in-depth study of this type of composite active matter.
Its transport properties result from the interactions of an active element (cell) and a passive one (cargo) and reveal an optimal cargo size that enhances the locomotion of the load-carrying cells, even exceeding their motility in the absence of cargo.
The experimental findings are rationalized in terms of a biohybrid active particle model that describes the emergent cell-cargo dynamics and enables us to derive the long-time diffusive transport of amoeboid microcarriers.
As amoeboid locomotion is commonly observed for mammalian cells such as leukocytes, our results lay the foundations for the study of transport performance of other medically relevant cell types and for extending our findings to more advanced transport tasks in complex environments, such as tissues.
This pre-registered study examined the prevalence and correlates of sexual aggression in a sample of 530 Iranians (322 women, 208 men) with a behaviorally specific questionnaire distinguishing between different coercive strategies, victim-perpetrator relationships, and sexual acts. Significantly more women (63.0%) than men (51.0%) experienced at least one incident of sexual aggression victimization since the age of 15 years, and significantly more men (37.0%) than women (13.4%) reported at least one incident of sexual aggression perpetration. In women and men, the experience of child sexual abuse predicted sexual victimization and sexual aggression perpetration after the age of 15 years, both directly and indirectly through higher engagement in risky sexual behavior. Greater endorsement of hostile masculinity among men explained additional variance in the prediction of sexual aggression perpetration. This research is a first step towards documenting and explaining high rates of sexual aggression victimization and perpetration among Iranian women and men, providing important information for sex education as well for the prevention of sexual aggression. However, to achieve these goals, we highlight the need for systematic actions in all educational, social, and legal sectors of Iranian society.
During a survey of aquatic fungi from Anzali Lagoon in Iran, several fungal specimens were isolated from freshwater habitats. Morphological evidence and comparing sequencing based on rDNA (ITS and LSU) and protein-coding genes (TEF1 and TUB2) showed that some isolates belong to undescribed fungal species.
These isolates belong to Arthrobotrys and Sarocladium, two ascomycetes genera. Arthrobotrys hyrcanus, sp. nov., differs from closely related species such as A. dianchiensis by its larger conidia and septation of primary conidia. Sarocladium pseudokiliense, sp. nov., was similar to S. kiliense, but distinguished by its conidial shape and the absence of adelophialides and chlamydospores.
Morphological descriptions, illustrations and multilocus phylogenetic analysis for both new species are provided.
Here, we establish different ways of light-triggered droplet manipulation such as reversible wetting, splitting, merging, and transport.
The unique features of our approach are that the changes in the wetting properties of microscopic droplets of isotropic (oil) or anisotropic (liquid crystalline) liquids adsorbed on photoswitchable films can be triggered just by application of soft optical stimuli, which lead to dynamical, reversible changes in the local morphology of the structured surfaces.
The adaptive films consist of an azobenzene-containing surfactant ionically attached to oppositely charged polymer chains. Under exposure to irradiation with light, the azobenzene photoisomerizes between two states, nonpolar trans -isomer and polar cis-isomer, resulting in the corresponding changes in the surface energy and orientation of the surfactant tails at the interface.
Additionally, the local increase in the surface temperature due to absorption of light by the azobenzene groups enables diverse processes of manipulation of the adsorbed small droplets, such as the reversible increase of the droplet basal area up to 5 times, anisotropic wetting during irradiation with modulated light, and precise partition of the droplet into many small pieces, which can then be merged on demand to the desired number of larger droplets.
Moreover, using a moving focused light spot, we experimentally demonstrate and theoretically explain the locomotion of the droplet over macroscopic distances with a velocity of up to 150 mu m center dot s-1.
Our findings could lead to the ultimate application of a programmable workbench for manipulating and operating an ensemble of droplets, just using simple and gentle optical stimuli.
Meropenem is one of the most frequently used antibiotics to treat life-threatening infections in critically ill patients.
This study aimed to develop a meropenem dosing algorithm for the treatment of Gram-negative infections based on intensive care unit (ICU)-specific resistance data. Antimicrobial susceptibility testing of Gram-negative bacteria obtained from critically ill patients was carried out from 2016 to 2020 at a tertiary care hospital.
Based on the observed MIC distribution, stochastic simulations (n = 1,000) of an evaluated pharmacokinetic meropenem model, and a defined pharmacokinetic/pharmacodynamic target (100%T->4xMIC while minimum concentrations were <44.5 mg/L), dosing recommendations for patients with varying renal function were derived.
Pathogen-specific MIC distributions were used to calculate the cumulative fraction of response (CFR), and the overall MIC distribution was used to calculate the local pathogen-independent mean fraction of response (LPIFR) for the investigated dosing regimens.
A CFR/LPIFR of >90% was considered adequate. The observed MIC distribution significantly differed from the EUCAST database. Based on the 6,520 MIC values included, a three-level dosing algorithm was developed.
If the pathogen causing the infection is unknown (level 1), known (level 2), known to be neither Pseudomonas aeruginosa nor Acinetobacrer baumannii, or classified as susceptible (level 3), a continuous infusion of 1.5 g daily reached sufficient target attainment independent of renal function.
In all other cases, dosing needs to be adjusted based on renal function. ICU-specific susceptibility data should be assessed regularly and integrated into dosing decisions. The presented workflow may serve as a blueprint for other antimicrobial settings.
Devising optimal interventions for constraining stochastic systems is a challenging endeavor that has to confront the interplay between randomness and dynamical nonlinearity.
Existing intervention methods that employ stochastic path sampling scale poorly with increasing system dimension and are slow to converge.
Here we propose a generally applicable and practically feasible methodology that computes the optimal interventions in a noniterative scheme.
We formulate the optimal dynamical adjustments in terms of deterministically sampled probability flows approximated by an interacting particle system.
Applied to several biologically inspired models, we demonstrate that our method provides the necessary optimal controls in settings with terminal, transient, or generalized collective state constraints and arbitrary system dynamics.
Unspecific peroxygenases (UPOs, EC 1.11.2.1) are fungal enzymes that catalyze the oxyfunctionalization of non-activated hydrocarbons, making them valuable biocatalysts. Despite the increasing interest in UPOs that has led to the identification of thousands of putative UPO genes, only a few of these have been successfully expressed and characterized.
There is currently no universal expression system in place to explore their full potential. Cell-free protein synthesis has proven to be a sophisticated technique for the synthesis of difficult-to-express proteins.
In this work, we aimed to establish an insect-based cell-free protein synthesis (CFPS) platform to produce UPOs. CFPS relies on translationally active cell lysates rather than living cells.
The system parameters can thus be directly manipulated without having to account for cell viability, thereby making it highly adaptable.
The insect-based lysate contains translocationally active, ER-derived vesicles, called microsomes.
These microsomes have been shown to allow efficient translocation of proteins into their lumen, promoting post-translational modifications such as disulfide bridge formation and N-glycosylations.
In this study the ability of a redox optimized, vesicle-based, eukaryotic CFPS system to synthesize functional UPOs was explored. The influence of different reaction parameters as well as the influence of translocation on enzyme activity was evaluated for a short UPO from Marasmius rotula and a long UPO from Agrocybe aegerita.
The capability of the CFPS system described here was demonstrated by the successful synthesis of a novel UPO from Podospora anserina, thus qualifying CFPS as a promising tool for the identification and evaluation of novel UPOs and variants thereof.
Quantifying the influence of pollen aging on the adhesive
properties of Hypochaeris radicata pollen
(2022)
Simple Summary Pollination is the transfer of pollen from a plant's male part (anther) to the corresponding female part (stigma). It is a fundamental biological process that ensures plant reproduction. Most studies investigate pollination from a biological perspective, but the underlying physical processes are poorly understood. Many plants rely on insects to transport pollen and the forces with which pollen adhere to insects and floral surfaces are fundamental for successful pollination.
We quantified pollen adhesion by measuring the forces necessary to detach Hypochaeris radicata (catsear, a common insect-pollinated plant) pollen from glass and studied for the first time how the adhesion forces change with pollen aging.
Our results show that newly formed adhesion bonds between H. radicata pollen and glass are stronger for fresh pollen than for old ones. On the other hand, when H. radicata pollen age in contact with glass, the adhesion between pollen and glass strengthens over time. These effects are probably caused by the viscous liquid covering most pollen (pollenkitt) changing its viscoelastic properties as it dries.
Although pollination is one of the most crucial biological processes that ensures plant reproduction, its mechanisms are poorly understood. Especially in insect-mediated pollination, a pollen undergoes several attachment and detachment cycles when being transferred from anther to insect and from insect to stigma. The influence of the properties of pollen, insect and floral surfaces on the adhesion forces that mediate pollen transfer have been poorly studied.
Here, we investigate the adhesive properties of Hypochaeris radicata pollen and their dependence on pollen aging by quantifying the pull-off forces from glass slides using centrifugation and atomic force microscopy. We found that the properties of the pollenkitt-the viscous, lipid liquid on the surface of most pollen grains-influences the forces necessary to detach a pollen from hydrophilic surfaces.
Our results show that aged H. radicata pollen form weaker adhesions to hydrophilic glass than fresh ones. On the other hand, when a pollen grain ages in contact with glass, the adhesion between the two surfaces increases over time.
This study shows for the first time the pollen aging effect on the pollination mechanism.
Volatile organic compounds (VOCs) are involved in microbial interspecies communication and in the mode of action of various antagonistic interactions. They are important for balancing host-microbe interactions and provide the basis for developing biological control strategies to control plant pathogens.
We studied the interactions between the bacterial antagonist Serratia plymuthica HRO-C48 and three fungal plant pathogens Rhizoctonia solani, Leptosphaeria maculans and Verticillium longisporum. Significant differences in fungal growth inhibition by the Serratia-emitted VOCs in pairwise dual culture assays and changes in the transcriptome of the bacterium and in the volatilomes of both interacting partners were observed. Even though the rate of fungal growth inhibition by Serratia was variable, the confrontation of the bacterium with the VOCs of all three fungi changed the levels of expression of the genes involved in stress response, biofilm formation, and the production of antimicrobial VOCs. Pairwise interacting microorganisms switched between defense (downregulation of gene expression) and attack (upregulation of gene expression and metabolism followed by growth inhibition of the interacting partner) modes, subject to the combinations of microorganisms that were interacting.
In the attack mode HRO-C48 significantly inhibited the growth of R. solani while simultaneously boosting its own metabolism; by contrast, its metabolism was downregulated when HRO-C48 went into a defense mode that was induced by the L. maculans and V. longisporum VOCs. L. maculans growth was slightly reduced by the one bacterial VOC methyl acetate that induced a strong downregulation of expression of genes involved in almost all metabolic functions in S. plymuthica.
Similarly, the interaction between S. plymuthica and V. longisporum resulted in an insignificant growth reduction of the fungus and repressed the rate of bacterial metabolism on the transcriptional level, accompanied by an intense volatile dialogue. Overall, our results indicate that VOCs substantially contribute to the highly break species-specific interactions between pathogens and their natural antagonists and thus deserving of increased consideration for pathogen control.