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The use of unilateral force under George W. Bush is not a new phenomenon in US foreign policy. As the author argues, it is merely a continuation of Bill Clinton’s foreign policy and is deeply rooted in both the foreign policy traditions of Jacksonianism and Wilsonianism. The analysis concludes that Clinton used unilateralist foreign policy with a 'smile' whereas the Bush administration uses it with an attitude.
In this work, some new results to exploit the recurrence properties of quasiperiodic dynamical systems are presented by means of a two dimensional visualization technique, Recurrence Plots(RPs). Quasiperiodicity is the simplest form of dynamics exhibiting nontrivial recurrences, which are common in many nonlinear systems. The concept of recurrence was introduced to study the restricted three body problem and it is very useful for the characterization of nonlinear systems. I have analyzed in detail the recurrence patterns of systems with quasiperiodic dynamics both analytically and numerically. Based on a theoretical analysis, I have proposed a new procedure to distinguish quasiperiodic dynamics from chaos. This algorithm is particular useful in the analysis of short time series. Furthermore, this approach demonstrates to be efficient in recognizing regular and chaotic trajectories of dynamical systems with mixed phase space. Regarding the application to real situations, I have shown the capability and validity of this method by analyzing time series from fluid experiments.
Contrastive focus
(2007)
The article puts forward a discourse-pragmatic approach to the notoriously evasive phenomena of contrastivity and emphasis. It is argued that occurrences of focus that are treated in terms of ‘contrastive focus’, ‘kontrast’ (Vallduví & Vilkuna 1998) or ‘identificational focus’ (É. Kiss 1998) in the literature should not be analyzed in familiar semantic terms like introduction of alternatives or exhaustivity. Rather, an adequate analysis must take into account discourse-pragmatic notions like hearer expectation or discourse expectability of the focused content in a given discourse situation. The less expected a given content is judged to be for the hearer, relative to the Common Ground, the more likely a speaker is to mark this content by means of special grammatical devices, giving rise to emphasis.
As land-cover conversion continues to expand into ever more remote areas in the humid tropics, montane rainforests are increasingly threatened. In the south Ecuadorian Andes, they are not only subject to man-made disturbances but also to naturally occurring landslides. I was interested in the impact of this ecosystem dynamics on a key parameter of the hydrologic cycle, the soil saturated hydraulic conductivity (synonym: permeability; Ks from here on), because it is a sensitive indicator for soil disturbances. My general objective was to quantify the effects of the regional natural and human disturbances on the saturated hydraulic conductivity and to describe the resulting spatial-temporal patterns. The main hypotheses were: 1) disturbances cause an apparent displacement of the less permeable soil layer towards the surface, either due to a loss of the permeable surface soil after land-sliding, or as a consequence of the surface soil compaction under cattle pastures; 2) ‘recovery’ from disturbance, either because of landslide re-vegetation or because of secondary succession after pasture abandonment, involves an apparent displacement of the less permeable layer back towards the original depth an 3) disturbances cause a simplification of the Ks spatial structure, i.e. the spatially dependent random variation diminishes; the subsequent recovery entails the re-establishment of the original structure. In my first study, I developed a synthesis of recent geostatistical research regarding its applicability to soil hydraulic data, including exploratory data analysis and variogram estimation techniques; I subsequently evaluated the results in terms of spatial prediction uncertainty. Concerning the exploratory data analysis, my main results were: 1) Gaussian uni- and bivariate distributions of the log-transformed data; 2) the existence of significant local trends; 3) no need for robust estimation; 4) no anisotropic variation. I found partly considerable differences in covariance parameters resulting from different variogram estimation techniques, which, in the framework of spatial prediction, were mainly reflected in the spatial connectivity of the Ks-field. Ignoring the trend component and an arbitrary use of robust estimators, however, would have the most severe consequences in this respect. Regarding variogram modeling, I encouraged restricted maximum likelihood estimation because of its accuracy and independence on the selected lags needed for experimental variograms. The second study dealt with the Ks spatial-temporal pattern in the sequences of natural and man-made disturbances characteristic for the montane rainforest study area. To investigate the disturbance effects both on global means and the spatial structure of Ks, a combined design-and model-based sampling approach was used for field-measurements at soil depths of 12.5, 20, and 50 cm (n=30-150/depth) under landslides of different ages (2 and 8 years), under actively grazed pasture, fallows following pasture abandonment (2 to 25 years of age), and under natural forest. Concerning global means, our main findings were 1) global means of the soil permeability generally decrease with increasing soil depth; 2) no significant Ks differences can be observed among landslides and compared to the natural forest; 3) a distinct permeability decrease of two orders of magnitude occurs after forest conversion to pasture at shallow soil depths, and 4) the slow regeneration process after pasture abandonment requires at least one decade. Regarding the Ks spatial structure, we found that 1) disturbances affect the Ks spatial structure in the topsoil, and 2) the largest differences in spatial patterns are associated with the subsoil permeability. In summary, the regional landslide activity seems to affect soil hydrology to a marginal extend only, which is in contrast to the pronounced drop of Ks after forest conversion. We used this spatial-temporal information combined with local rain intensities to assess the partitioning of rainfall into vertical and lateral flowpaths under undisturbed, disturbed, and regenerating land-cover types in the third study. It turned out that 1) the montane rainforest is characterized by prevailing vertical flowpaths in the topsoil, which can switch to lateral directions below 20 cm depth for a small number of rain events, which may, however, transport a high portion of the annual runoff; 2) similar hydrological flowpaths occur under the landslides except for a somewhat higher probability of impermeable layer formation in the topsoil of a young landslide, and 3) pronounced differences in runoff components can be observed for the human disturbance sequence involving the development of near-surface impeding layers for 24, 44, and 8 % of rain events for pasture, a two-year-old fallow, and a ten-year-old fallow, respectively.
We compute the local spectrum of the magnetic field near a metallic microstructure at finite temperature. Our main focus is on deviations from a plane-layered geometry for which we review the main properties. Arbitrary geometries are handled with the help of numerical calculations based on surface integral equations. The magnetic noise shows a significant polarization anisotropy above flat wires with finite lateral width, in stark contrast to an infinitely wide wire. Within the limits of a two-dimensional setting, our results provide accurate estimates for loss and dephasing rates in so-called `atom chip traps' based on metallic wires. A simple approximation based on the incoherent summation of local current elements gives qualitative agreement with the numerics, but fails to describe current correlations among neighboring objects.
The mammalian brain is, with its numerous neural elements and structured complex connectivity, one of the most complex systems in nature. Recently, large-scale corticocortical connectivities, both structural and functional, have received a great deal of research attention, especially using the approach of complex networks. Here, we try to shed some light on the relationship between structural and functional connectivities by studying synchronization dynamics in a realistic anatomical network of cat cortical connectivity. We model the cortical areas by a subnetwork of interacting excitable neurons (multilevel model) and by a neural mass model (population model). With weak couplings, the multilevel model displays biologically plausible dynamics and the synchronization patterns reveal a hierarchical cluster organization in the network structure. We can identify a group of brain areas involved in multifunctional tasks by comparing the dynamical clusters to the topological communities of the network. With strong couplings of multilevel model and by using neural mass model, the dynamics are characterized by well-defined oscillations. The synchronization patterns are mainly determined by the node intensity (total input strengths of a node); the detailed network topology is of secondary importance. The biologically improved multilevel model exhibits similar dynamical patterns in the two regimes. Thus, the study of synchronization in a multilevel complex network model of cortex can provide insights into the relationship between network topology and functional organization of complex brain networks.
[1] Observations of hydrological response often exhibit considerable scatter that is difficult to interpret. In this paper, we examine runoff production of 53 sprinkling experiments on the water-repellent soils in the southern Alps of Switzerland; simulated plot scale tracer transport in the macroporous soils at the Weiherbach site, Germany; and runoff generation data from the 2.3-km(2) Tannhausen catchment, Germany, that has cracking soils. The response at the three sites is highly dependent on the initial soil moisture state as a result of the threshold dynamics of the systems. A simple statistical model of threshold behavior is proposed to help interpret the scatter in the observations. Specifically, the model portrays how the inherent macrostate uncertainty of initial soil moisture translates into the scatter of the observed system response. The statistical model is then used to explore the asymptotic pattern of predictability when increasing the number of observations, which is normally not possible in a field study. Although the physical and chemical mechanisms of the processes at the three sites are different, the predictability patterns are remarkably similar. Predictability is smallest when the system state is close to the threshold and increases as the system state moves away from it. There is inherent uncertainty in the response data that is not measurement error but is related to the observability of the initial conditions.
CAMPHOR: A GOOD MODEL FOR ILLUSTRATING NMR TECHNIQUES. The use of Nuclear Magnetic Resonance spectroscopy to establish the three-dimensional structures of molecules is an important component of modern Chemistry courses. The combination of techniques that can be used for this purpose is conveniently illustrated by their application to the camphor molecule. This paper presents applications of several techniques used in NMR spectral interpretation in an increasing order of complexity. The result of individual experiments is illustrated in order to familiarize the user with the way connectivity through bonds and through space is established from 1D/2D-NMR spectra and molecular stereochemistry is determined from different NMR experiments
In this paper, we discuss the global existence of solutions for Chemotaxis models with saturation growth. If the coe±cients of the equations are all positive smooth T-periodic functions, then the problem has a positive T-periodic solution, and meanwhile we discuss here the stability problems for the T-periodic solutions.
In this article we construct the fundamental solutions for the wave equation arising in the de Sitter model of the universe. We use the fundamental solutions to represent solutions of the Cauchy problem and to prove the Lp − Lq-decay estimates for the solutions of the equation with and without a source term.
The intergalactic medium is kept highly photoionised by the intergalactic UV background radiation field generated by the overall population of quasars and galaxies. In the vicinity of sources of UV photons, such as luminous high-redshift quasars, the UV radiation field is enhanced due to the local source contribution. The higher degree of ionisation is visible as a reduced line density or generally as a decreased level of absorption in the Lyman alpha forest of neutral hydrogen. This so-called proximity effect has been detected with high statistical significance towards luminous quasars. If quasars radiate rather isotropically, background quasar sightlines located near foreground quasars should show a region of decreased Lyman alpha absorption close to the foreground quasar. Despite considerable effort, such a transverse proximity effect has only been detected in a few cases. So far, studies of the transverse proximity effect were mostly limited by the small number of suitable projected pairs or groups of high-redshift quasars. With the aim to substantially increase the number of quasar groups in the vicinity of bright quasars we conduct a targeted survey for faint quasars around 18 well-studied quasars at employing slitless spectroscopy. Among the reduced and calibrated slitless spectra of 29000 objects on a total area of 4.39 square degrees we discover in total 169 previously unknown quasar candidates based on their prominent emission lines. 81 potential z>1.7 quasars are selected for confirmation by slit spectroscopy at the Very Large Telescope (VLT). We are able to confirm 80 of these. 64 of the newly discovered quasars reside at z>1.7. The high success rate of the follow-up observations implies that the majority of the remaining candidates are quasars as well. In 16 of these groups we search for a transverse proximity effect as a systematic underdensity in the HI Lyman alpha absorption. We employ a novel technique to characterise the random absorption fluctuations in the forest in order to estimate the significance of the transverse proximity effect. Neither low-resolution spectra nor high-resolution spectra of background quasars of our groups present evidence for a transverse proximity effect. However, via Monte Carlo simulations the effect should be detectable only at the 1-2sigma level near three of the foreground quasars. Thus, we cannot distinguish between the presence or absence of a weak signature of the transverse proximity effect. The systematic effects of quasar variability, quasar anisotopy and intrinsic overdensities near quasars likely explain the apparent lack of the transverse proximity effect. Even in absence of the systematic effects, we show that a statistically significant detection of the transverse proximity effect requires at least 5 medium-resolution quasar spectra of background quasars near foreground quasars whose UV flux exceeds the UV background by a factor 3. Therefore, statistical studies of the transverse proximity effect require large numbers of suitable pairs. Two sightlines towards the central quasars of our survey fields show intergalactic HeII Lyman alpha absorption. A comparison of the HeII absorption to the corresponding HI absorption yields an estimate of the spectral shape of the intergalactic UV radiation field, typically parameterised by the HeII/HI column density ratio eta. We analyse the fluctuating UV spectral shape on both lines of sight and correlate it with seven foreground quasars. On the line of sight towards Q0302-003 we find a harder radiation field near 4 foreground quasars. In the direct vicinity of the quasars eta is consistent with values of 25-100, whereas at large distances from the quasars eta>200 is required. The second line of sight towards HE2347-4342 probes lower redshifts where eta is directly measurable in the resolved HeII forest. Again we find that the radiation field near the 3 foreground quasars is significantly harder than in general. While eta still shows large fluctuations near the quasars, probably due to radiative transfer, the radiation field is on average harder near the quasars than far away from them. We interpret these discoveries as the first detections of the transverse proximity effect as a local hardness fluctuation in the UV spectral shape. No significant HI proximity effect is predicted for the 7 foreground quasars. In fact, the HI absorption near the quasars is close to or slightly above the average, suggesting that the weak signature of the transverse proximity effect is masked by intrinsic overdensities. However, we show that the UV spectral shape traces the transverse proximity effect even in overdense regions or at large distances. Therefore, the spectral hardness is a sensitive physical measure of the transverse proximity effect that is able to break the density degeneracy affecting the traditional searches.
Electrically charged cellular polymer films can exhibit very high piezoelectric activity and are therefore more and more often employed in advanced electromechanical and electro-acoustical transducers. In this paper, we report an optimized sequence of steps for preparing such ferroelectrets from commercial nonvoided ploy(ethylene terephthalate) (PETP) films by means of foaming with CO2 biaxial mechanical stretching, controlled void inflation, and bipolar electric charging. The nonvoid PETP films foamed with supercritical CO2 at a suitably high pressure and subsequently annealed for stabilization. The cellular foam structure was further optimized by means of well controlled biaxial stretching in a commercial stretcher and sometimes subsequent inflamation in a pressure chamber. Bipolar electric charging of the internal voids was achieved through the application of high electric fields in an SF0 atmosphere. The new optimized PETP ferroelectric exhibit quite large piezoelectric coefficients up to almost 500 pCN(-1), for which unusually low elastic stiffness of only around 0.3 MPa are essential. The PETP foam ferroelectrics posses unclamped thickenss-extension resonance frequences between approximately 120 and 250 kHz, and are thus highly suitable for several established as well as novel ultrasonic-transductant applications.
The paper presents a simulation and parameter-estimation approach for evaluating stochastic patterns of population growth and spread of an annual forest herb, Melampyrum pratense (Orobanchaceae). The survival of a species during large-scale changes in land use and climate will depend, to a considerable extent, on its dispersal and colonisation abilities. Predictions on species migration need a combination of field studies and modelling efforts. Our study on the ability of M. pratense to disperse into so far unoccupied areas is based on experiments in secondary woodland in NE Germany. Experiments started in 1997 at three sites where the species was not yet present, with 300 seeds sown within 1m2. Population development was then recorded until 2001 by mapping of individuals with a resolution of 5 cm. Additional observations considered density dependence of seed production. We designed a spatially explicit individual-based computer simulation model to explain the spatial patterns of population development and to predict future population spread. Besides primary drop of seeds (barochory) it assumed secondary seed transport by ants (myrmecochory) with an exponentially decreasing dispersal tail. An important feature of population-pattern explanation was the simultaneous estimation of both population-growth and dispersal parameters from consistent spatio-temporal data sets. As the simulation model produced stochastic time series and random spatially discrete distributions of individuals we estimated parameters by minimising the expectation of weighted sum of squares. These sums of squares criteria considered population sizes, radial population distributions around the area of origin and distributions of individuals within squares of 25cm×25 cm, the range of density action. Optimal parameter values, together with the precision of the estimates, were obtained from calculating sum of squares in regular grids of parameter values. Our modelling results showed that transport of fractions of seeds by ants over distances of 1-2m was indispensable for explaining the observed population spread that led to distances of at most 8mfrom population origin within 3 years. Projections of population development over four additional years gave a diffusion-like increase of population area without any "outposts". This prediction generated by the simulation model gave a hypothesis which should be revised by additional field observations. Some structural deviations between observations and model output already indicated that for full understanding of population spread the set of dispersal mechanisms assumed in the model may have to be extended by additional features of plant-animal mutualism.
The paper presents a simulation and parameter-estimation approach for evaluating stochastic patterns of population growth and spread of an annual forest herb, Melampyrum pratense (Orobanchaceae). The survival of a species during large-scale changes in land use and climate will depend, to a considerable extent, on its dispersal and colonisation abilities. Predictions on species migration need a combination of field studies and modelling efforts. Our study on the ability of M. pratense to disperse into so far unoccupied areas was based on experiments in secondary woodland in NE Germany. Experiments started in 1997 at three sites where the species was not yet present, with 300 seeds sown within one square meter. Population development was then recorded until 2001 by mapping of individuals with a resolution of 5 cm. Additional observations considered density dependence of seed production. We designed a spatially explicit individual-based computer simulation model to explain the spatial patterns of population development and to predict future population spread. Besides primary drop of seeds (barochory) it assumed secondary seed transport by ants (myrmecochory) with an exponentially decreasing dispersal tail. An important feature of populationpattern explanation was the simultaneous estimation of both population-growth and dispersal parameters from consistent spatio-temporal data sets. As the simulation model produced stochastic time series and random spatially discrete distributions of individuals we estimated parameters by minimising the expectation of weighted sums of squares. These sums-ofsquares criteria considered population sizes, radial population distributions around the area of origin and distributions of individuals within squares of 25*25 cm, the range of density action. Optimal parameter values, together with the precision of the estimates, were obtained from calculating sums of squares in regular grids of parameter values. Our modelling results showed that transport of fractions of seeds by ants over distances of 1…2 m was indispensable for explaining the observed population spread that led to distances of at most 8 m from population origin within 3 years. Projections of population development over 4 additional years gave a diffusion-like increase of population area without any “outposts”. This prediction generated by the simulation model gave a hypothesis which should be revised by additional field observations. Some structural deviations between observations and model output already indicated that for full understanding of population spread the set of dispersal mechanisms assumed in the model may have to be extended by additional features of plant-animal mutualism.
There is already strong evidence that temperate lakes have been highly vulnerable to human induced climate warming during the last century. Hitherto climate impact studies have mainly focussed on the impacts of the recent long-term warming in winter and spring and little is known on the influence of climate warming on temperate lakes in summer. In the present thesis, I studied some aspects, which may have been strongly involved in determining the response of a lake to climate warming in summer. Thereby I have focussed on climate induced impacts on the thermal characteristics and the phenology and abundance of summer plankton in a shallow polymictic lake (Müggelsee, Germany). First, the influence of climate warming on the phenology and abundance of the lake plankton was investigated across seasons. Fast-growing spring phytoplankton and zooplankton (Daphnia) advanced largely synchronously, whereas long-term changes in the phenology of slow-growing summer zooplankton were clearly species-specific and not synchronised. The phenology and/or abundance of several summer copepod species changed according to their individual thermal requirements at decisive developmental stages such as emergence from diapause in spring. The study emphasises that not only the degree of warming, but also its timing within the annual cycle is of great ecological importance. To analyse the impact of climate change on the thermal characteristics of the lake, I examined the long-term development of the daily epilimnetic temperature extrema during summer. The study demonstrated for the first time for lakes that the daily epilimnetic minima (during nighttime) have increased more rapidly than the daily epilimnetic maxima (during daytime), resulting in a distinct decrease in the daily epilimnetic temperature range. This day-night asymmetry in epilimnetic temperature was likely caused by an increased nighttime emission of long-wave radiation from the atmosphere. This underlines that not only increases in air temperature, but also changes in other meteorological variables such as wind speed, relative humidity and cloud cover may play an important role in determining the lake temperature with respect to further climate change. Furthermore, a short-term analysis on the mixing regime of the polymictic lake was conducted to examine the frequency and duration of stratification events and their impacts on dissolved oxygen, dissolved nutrients and summer phytoplankton. Even during the longest stratification events (heatwaves in 2003 and 2006) the thermal characteristics of the lake differed from those typically found in shallow dimictic lakes, which exhibit a continuous stratification during summer. Particularly, hypolimnetic temperatures were higher, favouring the depletion of oxygen and the accumulation of dissolved nutrient in the hypolimnion. Thermal stratification will be very likely amplified in the future, thus, I conclude that polymictic lakes will be very vulnerable to alterations in the thermal regime with respect to projections of further climate change during summer. Finally, a long-term case study on the long and short-term changes in the development of the planktonic larvae of the freshwater mussel Dreissena polymorpha was performed to analyse the impacts of simultaneous changes in the thermal and in the trophic regime of the lake. Both the climate warming and the decrease in external nutrient load were important in determining the abundance of the pelagic larvae by affecting different features of the life-history of this species throughout the warm season. The long-term increase in the abundance and length of larvae was related to the decrease in external nutrient loading and the change in phytoplankton composition. However, the recent heatwaves in 2003 and 2006 have offset this positive effect on larval abundance, due to unfavourable low oxygen concentrations that had resulted from extremely long stratification events, mimicking the effects of nutrient enrichment. Climate warming may thus induce counteracting effects in productive shallow lakes that underwent lake restoration through a decrease in external nutrient loading. I conclude that not only the nature of climate change and thus the timing of climate warming throughout the seasons and the occurrence of climatic extremes as heatwaves, but also site-specific lake conditions as the thermal mixing regime and the trophic state are crucial factors governing the impacts of climate warming on internal lake processes during summer. Consequently, further climate impact research on lake functioning should focus on how the different lake types respond to the complex environmental forcing in summer, to allow for a comprehensive understanding of human induced environmental changes in lakes.
A tight hormonal control of energy homeostasis is of pivotal relevance for animals. Recent evidence suggests an involvement of the nuclear receptor NR1i3 (CAR). Fasting induces CAR by largely unknown mechanisms and CAR-deficient mice are defective in fasting adaptation. In rat hepatocytes CAR was induced by WY14643, a PPARalpha-agonist. A DR1 motif in the CAR promoter was necessary and sufficient for this control. The PPARalpha-dependent increase in CAR potentiated the phenobarbital-induced transcription of the prototypical CAR-dependent gene CYP2B1. Since free fatty acids are natural ligands for PPARalpha, a fasting-induced increase in free fatty acids might induce CAR. In accordance with this hypothesis, CAR induction by fasting was abrogated in PPARalpha-deficient mice.
Different habitat models were created for the White Stork (Ciconia ciconia) in the region of the former German province of East Prussia (equals app. the current Russian oblast Kaliningrad and the Polish voivodship Warmia-Masuria). Different historical data sets describing the occurrence of the White Stork in the 1930s, as well as selected variables for the description of landscape and habitat, were employed. The processing and modeling of the applied data sets was done with a geographical information system (ArcGIS) and a statistical modeling approach that comes from the disciplines of machine-learning and data mining (TreeNet by Salford Systems Ltd.). Applying historical habitat descriptors, as well as data on the occurrence of the White Stork, models on two different scales were created: (i) a point scale model applying a raster with a cell size of 1 km2 and (ii) an administrative district scale model based on the organization of the former province of East Prussia. The evaluation of the created models show that the occurrence of White Stork nesting grounds in the former East Prussia for most parts is defined by the variables ‘forest’, ‘settlement area’, ‘pasture land’ and ‘proximity to coastline’. From this set of variables it can be assumed that a good food supply and nesting opportunities are provided to the White Stork in pasture and meadows as well as in the proximity to human settlements. These could be seen as crucial factors for the choice of nesting White Stork in East Prussia. Dense forest areas appear to be unsuited as nesting grounds of White Storks. The high influence of the variable ‘coastline’ is most likely explained by the specific landscape composition of East Prussia parallel to the coastline and is to be seen as a proximal factor for explaining the distribution of breeding White Storks. In a second step, predictions for the period of 1981 to 1993 could be made applying both scales of the models created in this study. In doing so, a decline of potential nesting habitat was predicted on the point scale. In contrast, the predicted White Stork occurrence increases when applying the model of the administrative district scale. The difference between both predictions is to be seen in the application of different scales (density versus suitability as breeding ground) and partly dissimilar explanatory variables. More studies are needed to investigate this phenomenon. The model predictions for the period 1981 to 1993 could be compared to the available inventories of that period. It shows that the figures predicted here were higher than the figures established by the census. This means that the models created here show rather a capacity of the habitat (potential niche). Other factors affecting the population size e.g. breeding success or mortality have to be investigated further. A feasible approach on how to generate possible habitat models was shown employing the methods presented here and applying historical data as well as assessing the effects of changes in land use on the White Stork. The models present the first of their kind, and could be improved by means of further data regarding the structure of the habitat and more exact spatially explicit information on the location of the nesting sites of the White Stork. In a further step, a habitat model of the present times should be created. This would allow for a more precise comparison regarding the findings from the changes of land use and relevant conditions of the environment on the White Stork in the region of former East Prussia, e.g. in the light of coming landscape changes brought by the European Union (EU).
The mode of nutrition of mixotrophic flagellates determines the food quality for their consumers
(2007)
According to resource allocation theory, animals face a trade off between the allocation of resources into reproduction and into individual growth/maintenance. This trade off is reinforced when food conditions decline. It is well established in biological research that many animals increase their life span when food is in suboptimal supply for growth and/or reproduction. Such a situation of reduced food availability is called dietary restriction. An increase in life span under dietary restricted conditions is seen as a strategy to tolerate periods of food shortage so that the animals can start reproduction again when food is in greater supply. In this study, the effect of dietary restriction on life span and reproduction in two rotifer species, Cephalodella sp. and Elosa worallii, was investigated using life table experiments. The food concentration under dietary restricted conditions was below the threshold for population growth. It was (1) tested whether the rotifers start reproduction again after food replenishment, and (2) estimated whether the time scale of dietary restricted conditions is relevant for the persistence of a population in the field. Only E. worallii responded to dietary restriction with an increase in life span at the expense of reproduction. After replenishment of food, E. worallii started to reproduce again within I day. With an increase in the duration of dietary restricted conditions of up to 15 days, which is longer than the median life span of E. worallii under food saturation, the life span increased and the life time reproduction decreased. These results suggest that in a temporally (or spatially) variable environment, some rotifer populations can persist even during long periods of severe food deprivation.
The most massive stars are those with the shortest but most active life. One group of massive stars, the Luminous Blue Variables (LBVs), of which only a few objects are known, are in particular of interest concerning the stability of stars. They have a high mass loss rate and are close to being instable. This is even more likely as rotation becomes an important factor in stellar evolution of these stars. Through massive stellar winds and sometimes giant eruptions, LBV nebulae are formed. Various aspects in the evolution in the LBV phase lead, beside the large scale morphological and kinematical differences, to a diversity of small structures like clumps, rims, and outflows in these nebulae.
Proteins are chain molecules built from amino acids. The precise sequence of the 20 different types of amino acids in a protein chain defines into which structure a protein folds, and the three-dimensional structure in turn specifies the biological function of the protein. The reliable folding of proteins is a prerequisite for their robust function. Misfolding can lead to protein aggregates that cause severe diseases, such as Alzheimer's, Parkinson's, or the variant Creutzfeldt-Jakob disease. Small single-domain proteins often fold without experimentally detectable metastable intermediate states. The folding dynamics of these proteins is thought to be governed by a single transition-state barrier between the unfolded and the folded state. The transition state is highly instable and cannot be observed directly. However, mutations in which a single amino acid of the protein is substituted by another one can provide indirect access. The mutations slightly change the transition-state barrier and, thus, the folding and unfolding times of the protein. The central question is how to reconstruct the transition state from the observed changes in folding times. In this habilitation thesis, a novel method to extract structural information on transition states from mutational data is presented. The method is based on (i) the cooperativity of structural elements such as alpha-helices and beta-hairpins, and (ii) on splitting up mutation-induced free-energy changes into components for these elements. By fitting few parameters, the method reveals the degree of structure formation of alpha-helices and beta-hairpins in the transition state. In addition, it is shown in this thesis that the folding routes of small single-domain proteins are dominated by loop-closure dependencies between the structural elements.
In semi-arid savannah ecosystems, the vegetation structure and composition, i.e. the architecture of trees, shrubs, grass tussocks and herbaceous plants, offer a great variety of habitats and niches to sustain animal diversity. In the last decades intensive human land use practises like livestock farming have altered the vegetation in savannah ecosystems worldwide. Extensive grazing leads to a reduction of the perennial and herbaceous vegetation cover, which results in an increased availability of bare soil. Both, the missing competition with perennial grasses and the increase of bare soils favour shrub on open ground and lead to area-wide shrub encroachment. As a consequence of the altered vegetation structure and composition, the structural diversity declines. It has been shown that with decreasing structural diversity animal diversity decline across a variety of taxa. Knowledge on the effects of overgrazing on reptiles, which are an important part of the ecosystem, are missing. Furthermore, the impact of habitat degradation on factors of a species population dynamic and life history, e.g., birth rate, survival rate, predation risk, space requirements or behavioural adaptations are poorly known. Therefore, I investigated the impact of overgrazing on the reptile community in the southern Kalahari. Secondly I analysed population dynamics and the behaviour of the Spotted Sand Lizard, Pedioplanis l. lineoocellata. All four chapters clearly demonstrate that habitat degradation caused by overgrazing had a severe negative impact upon (i) the reptile community as a whole and (ii) on population parameters of Pedioplanis l. lineoocellata. Chapter one showed a significant decline of regional reptile diversity and abundance in degraded habitats. In chapter two I demonstrated that P. lineoocellata moves more frequently, spends more time moving and covers larger distances in degraded than in non-degraded habitats. In addition, home range size of the lizard species increases in degraded habitats as shown by chapter three. Finally, chapter four showed the negative impacts of overgrazing on several population parameters of P. lineoocellata. Absolute population size of adult and juvenile lizards, survival rate and birth rate are significantly lower in degraded habitats. Furthermore, the predation risk was greatly increased in degraded habitats. A combination of a variety of aspects can explain the negative impact of habitat degradation on reptiles. First, reduced prey availability negatively affects survival rate, the birth rate and overall abundance. Second, the loss of perennial plant cover leads to a loss of niches and to a reduction of opportunities to thermoregulate. Furthermore, a loss of cover and is associated with increased predation risk. A major finding of my thesis is that the lizard P. lineoocellata can alter its foraging strategy. Species that are able to adapt and change behaviour, such as P. lineoocellata can effectively buffer against changes in their environment. Furthermore, perennial grass cover can be seen as a crucial ecological component of the vegetation in the semi-arid savannah system of the southern Kalahari. If perennial grass cover is reduced to a certain degree reptile diversity will decline and most other aspects of reptile life history will be negatively influenced. Savannah systems are characterised by a mixture of trees, shrubs and perennial grasses. These three vegetation components determine the composition and structure of the vegetation and accordingly influence the faunal diversity. Trees are viewed as keystone structures and focal points of animal activity for a variety of species. Trees supply animals with shelter, shade and food and act as safe sites, nesting sites, observation posts and foraging sites. Recent research demonstrates a positive influence of shrub patches on animal diversity. Moreover, it would seem that intermediate shrub cover can also sustain viable populations in savannah landscapes as has been demonstrated for small carnivores and rodent species. The influence of perennial grasses on faunal diversity did not receive the same attention as the influence of trees and shrubs. In my thesis I didn’t explicitly measure the direct effects of perennial grasses but my results strongly imply that it has an important role. If the perennial grass cover is significantly depleted my results suggest it will negatively influence reptile diversity and abundance and on several populations parameters of P. lineoocellata. Perennial grass cover is associated with the highest prey abundance, reptile diversity and reptile abundance. It provides reptiles both a refuge from predators and opportunities to optimise thermoregulation. The relevance of each of the three vegetation structural elements is different for each taxa and species. In conclusion, I can all three major vegetation structures in the savannah system are important for faunal diversity.
INTEGRAL tripled the number of super-giant high-mass X-ray binaries (sgHMXB) known in the Galaxy by revealing absorbed and fast transient (SFXT) systems. Quantitative constraints on the wind clumping of massive stars can be obtained from the study of the hard X-ray variability of SFXT. A large fraction of the hard X-ray emission is emitted in the form of flares with a typical duration of 3 ksec, frequency of 7 days and luminosity of $10^{36}$ erg/s. Such flares are most probably emitted by the interaction of a compact object orbiting at $\sim10~R_*$ with wind clumps ($10^{22 ... 23}$ g) representing a large fraction of the stellar mass-loss rate. The density ratio between the clumps and the inter-clump medium is $10^{2 ... 4}$. The parameters of the clumps and of the inter-clump medium, derived from the SFXT flaring behavior, are in good agreement with macro-clumping scenario and line-driven instability simulations. SFXT are likely to have larger orbital radius than classical sgHMXB.
The Arctic plays a key role in Earth’s climate system as global warming is predicted to be most pronounced at high latitudes and because one third of the global carbon pool is stored in ecosystems of the northern latitudes. In order to improve our understanding of the present and future carbon dynamics in climate sensitive permafrost ecosystems, the present study concentrates on investigations of microbial controls of methane fluxes, on the activity and structure of the involved microbial communities, and on their response to changing environmental conditions. For this purpose an integrated research strategy was applied, which connects trace gas flux measurements to soil ecological characterisation of permafrost habitats and molecular ecological analyses of microbial populations. Furthermore, methanogenic archaea isolated from Siberian permafrost have been used as potential keystone organisms for studying and assessing life under extreme living conditions. Long-term studies on methane fluxes were carried out since 1998. These studies revealed considerable seasonal and spatial variations of methane emissions for the different landscape units ranging from 0 to 362 mg m-2 d-1. For the overall balance of methane emissions from the entire delta, the first land cover classification based on Landsat images was performed and applied for an upscaling of the methane flux data sets. The regionally weighted mean daily methane emissions of the Lena Delta (10 mg m-2 d-1) are only one fifth of the values calculated for other Arctic tundra environments. The calculated annual methane emission of the Lena Delta amounts to about 0.03 Tg. The low methane emission rates obtained in this study are the result of the used remotely sensed high-resolution data basis, which provides a more realistic estimation of the real methane emissions on a regional scale. Soil temperature and near soil surface atmospheric turbulence were identified as the driving parameters of methane emissions. A flux model based on these variables explained variations of the methane budget corresponding to continuous processes of microbial methane production and oxidation, and gas diffusion through soil and plants reasonably well. The results show that the Lena Delta contributes significantly to the global methane balance because of its extensive wetland areas. The microbiological investigations showed that permafrost soils are colonized by high numbers of microorganisms. The total biomass is comparable to temperate soil ecosystems. Activities of methanogens and methanotrophs differed significantly in their rates and distribution patterns along both the vertical profiles and the different investigated soils. The methane production rates varied between 0.3 and 38.9 nmol h-1 g-1, while the methane oxidation ranged from 0.2 to 7.0 nmol h-1 g-1. Phylogenetic analyses of methanogenic communities revealed a distinct diversity of methanogens affiliated to Methanomicrobiaceae, Methanosarcinaceae and Methanosaetaceae, which partly form four specific permafrost clusters. The results demonstrate the close relationship between methane fluxes and the fundamental microbiological processes in permafrost soils. The microorganisms do not only survive in their extreme habitat but also can be metabolic active under in situ conditions. It was shown that a slight increase of the temperature can lead to a substantial increase in methanogenic activity within perennially frozen deposits. In case of degradation, this would lead to an extensive expansion of the methane deposits with their subsequent impacts on total methane budget. Further studies on the stress response of methanogenic archaea, especially Methanosarcina SMA-21, isolated from Siberian permafrost, revealed an unexpected resistance of the microorganisms against unfavourable living conditions. A better adaptation to environmental stress was observed at 4 °C compared to 28 °C. For the first time it could be demonstrated that methanogenic archaea from terrestrial permafrost even survived simulated Martian conditions. The results show that permafrost methanogens are more resistant than methanogens from non-permafrost environments under Mars-like climate conditions. Microorganisms comparable to methanogens from terrestrial permafrost can be seen as one of the most likely candidates for life on Mars due to their physiological potential and metabolic specificity.
Both the stoichiometry and complex stability constants of crown ether complexes with metal ions have been determined by examining gradual changes in their diffusional behavior in nonaqueous solution. Diffusion coefficients, D, were evaluated by pulsed field gradient (PFG) NMR titration experiments whilst complex stability constants were determined by nonlinear curve-fitting procedures, D versus csol., which also allow the treatment of multiple complexation equilibria (1:1 to 1:2 stoichiometries). Differences in the diffusion coefficients of the various free crown ethers with respect to their metal ion complexes indicate great sensitivity to both conformational changes and changes in molecular size upon complexation.
An investigation of the conformational landscape of 1,3-dithian-2-yl bearing porphyrins and the rotational behavior of the dithianyl substituents in meso position was carried out by variable-temperature (VT) NMR spectroscopy. Additionally, theoretical results for alternative conformations and energy barriers were obtained by molecular modeling. The study revealed different NH trans tautomers with regard to the orientation of the dithianyl ligands for the free base porphyrins 1-3. Relatively ruffled porphyrin core conformations were established for the transition states of the dithianyl rotation, resulting in a lower rotational energy barrier for the nickel(II) complex 4 compared to that of the free base systems. The data obtained and the first depiction of a rotational transition state for the rotation of bulky meso-alkyl substituents illustrate the close structural interplay between meso-alkyl substituents and the macrocycle conformation in porphyrins.
Mafic magmatism in the Eastern Cordillera and Putumayo Basin, Colombia : causes and consequences
(2007)
The Eastern Cordillera of Colombia is mainly composed of sedimentary rocks deposited since early Mesozoic times. Magmatic rocks are scarce. They are represented only by a few locally restricted occurrences of dykes and sills of mafic composition presumably emplaced in the Cretaceous and of volcanic rocks of Neogene age. This work is focused on the study of the Cretaceous magmatism with the intention to understand the processes causing the genesis of these rocks and their significance in the regional tectonic setting of the Northern Andes. The magmatic rocks cut the Cretaceous sedimentary succession of black shales and marlstones that crop out in both flanks of the Eastern Cordillera. The studied rocks were classified as gabbros (Cáceres, Pacho, Rodrigoque), tonalites (Cáceres, La Corona), diorites and syenodiorites (La Corona), pyroxene-hornblende gabbros (Pacho), and pyroxene-hornblendites (Pajarito). The gabbroic samples are mainly composed of plagioclase, clinopyroxene, and/or green to brown hornblende, whereas the tonalitic rocks are mainly composed of plagioclase and quartz. The samples are highly variable in crystal sizes from fine- to coarse-grained. Accessory minerals such as biotite, titanite and zircon are present. Some samples are characterized by moderate to strong alteration, and show the presence of epidote, actinolite and chlorite. Major and trace element compositions of the rocks as well as the rock-forming minerals show significant differences in the geochemical and petrological characteristics for the different localities, suggesting that this magmatism does not result from a single melting process. The wide compositional spectrum of trace elements in the intrusions is characteristic for different degrees of mantle melting and enrichment of incompatible elements. MORB- and OIB-like compositions suggest at least two different sources of magma with tholeiitic and alkaline affinity, respectively. Evidence of slab-derived fluids can be recognized in the western part of the basin reflected in higher Ba/Nb and Sr/P ratios and also in the Sr radiogenic isotope ratios, which is possible a consequence of metasomatism in the mantle due to processes related to the presence of a previously subducted slab. The trace element patterns evidence an extensional setting in the Cretaceous basin producing a continental rift, with continental crust being stretched until oceanic crust was generated in the last stages of this extension. Electron microprobe analyses (EMPA) of the major elements and synchrotron radiation micro-X-ray fluorescence (μ-SRXRF) analyses of the trace element composition of the early crystallized minerals of the intrusions (clinopyroxenes and amphiboles) reflect the same dual character that has been found in the bulk-rock analyses. Despite the observed alteration of the rocks, the mineral composition shows evidences for an enriched and a relative depleted magma source. Even the normalization of the trace element concentrations of clinopyroxenes and amphiboles to the whole rock nearly follows the pattern predicted by published partition coefficients, suggesting that the alteration did not change the original trace element compositions of the investigated minerals. Sr-Nd-Pb isotope data reveal a large isotopic variation but still suggest an initial origin of the magmas in the mantle. Samples have moderate to highly radiogenic compositions of 143Nd/144Nd and high 87Sr/86Sr ratios and follow a trend towards enriched mantle compositions, like the local South American Paleozoic crust. The melts experienced variable degrees of contamination by sediments, crust, and seawater. The age corrected Pb isotope ratios show two separated groups of samples. This suggests that the chemical composition of the mantle below the Northern Andes has been modified by the interaction with other components resulting in a heterogeneous combination of materials of diverse origins. Although previous K/Ar age dating have shown that the magmatism took place in the Cretaceous, the high error of the analyses and the altered nature of the investigated minerals did preclude reliable interpretations. In the present work 40Ar/39Ar dating was carried out. The results show a prolonged history of magmatism during the Cretaceous over more than 60 Ma, from ~136 to ~74 Ma (Hauterivian to Campanian). Pre-Cretaceous rifting phases occurred in the Triassic-Jurassic for the western part of the basin and in the Paleozoic for the eastern part. Those previous rifting phases are decisive mechanisms controlling the localization and composition of the Cretaceous magmatism. Therefore, it is the structural position and not the age of the intrusions which preconditions the kind of magmatism and the degree of melting. The divergences on ages are the consequence of the segmentation of the basin in several sub-basins which stretching, thermal evolution and subsidence rate evolved independently. The first hypothesis formulated at the beginning of this investigation was that the Cretaceous gabbroic intrusions identified in northern Ecuador could be correlated with the intrusions described in the Eastern Cordillera. The mafic occurrences should mark the location of the most subsiding places of the large Cretaceous basin in northern South America. For this reason, the gabbroic intrusions cutting the Cretaceous succession in the Putumayo Basin, southern Colombia, were investigated. The results of the studies were quite unexpected. The petrologic and geochemical character of the magmatic rocks indicates subduction-related magmatism. K/Ar dating of amphibole yields a Late Miocene to Pliocene age (6.1 ± 0.7 Ma) for the igneous event in the basin. Although there is no correlation between this magmatic event and the Cretaceous magmatic event, the data obtained has significant tectonic and economic implications. The emplacement of the Neogene gabbroic rocks coincides with the late Miocene/Pliocene Andean orogenic uplift as well as with a significant pulse of hydrocarbon generation and expulsion.
We present the results of Monte Carlo mass-loss predictions for massive stars covering a wide range of stellar parameters. We critically test our predictions against a range of observed massloss rates – in light of the recent discussions on wind clumping. We also present a model to compute the clumping-induced polarimetric variability of hot stars and we compare this with observations of Luminous Blue Variables, for which polarimetric variability is larger than for O and Wolf-Rayet stars. Luminous Blue Variables comprise an ideal testbed for studies of wind clumping and wind geometry, as well as for wind strength calculations, and we propose they may be direct supernova progenitors.
Phonology and intonation
(2007)
Recent studies of massive O-type stars present clear evidences of inhomogeneous and clumped winds. O-type (H-rich) central stars of planetary nebulae (CSPNs) are in some ways the low mass–low luminosity analogous of those massive stars. In this contribution, we present preliminary results of our on-going multi-wavelength (FUV, UV and optical) study of the winds of Galactic CSPNs. Particular emphasis will be given to the clumping factors derived by means of optical lines (Hα and Heii 4686) and “classic” FUV (and UV) lines.
Magnetic fields influence the dynamics of hot-star winds and create large scale structure. Based on numerical magnetohydrodynamic (MHD) simulations, we model the wind of θ¹ Ori C, and then use the SEI method to compute synthetic line profiles for a range of viewing angles as function of rotational phase. The resulting dynamic spectrum for a moderately strong line shows a distinct modulation, but with a phase that seems at odds with available observations.
This work is concerned with the spatio-temporal structures that emerge when non-identical, diffusively coupled oscillators synchronize. It contains analytical results and their confirmation through extensive computer simulations. We use the Kuramoto model which reduces general oscillatory systems to phase dynamics. The symmetry of the coupling plays an important role for the formation of patterns. We have studied the ordering influence of an asymmetry (non-isochronicity) in the phase coupling function on the phase profile in synchronization and the intricate interplay between this asymmetry and the frequency heterogeneity in the system. The thesis is divided into three main parts. Chapter 2 and 3 introduce the basic model of Kuramoto and conditions for stable synchronization. In Chapter 4 we characterize the phase profiles in synchronization for various special cases and in an exponential approximation of the phase coupling function, which allows for an analytical treatment. Finally, in the third part (Chapter 5) we study the influence of non-isochronicity on the synchronization frequency in continuous, reaction diffusion systems and discrete networks of oscillators.
During the last decades, the global change of the environment has caused a dramatic loss of habitats and species. In Central Europe, open habitats are particularly affected. The main objective of this thesis was to experimentally test the suitability of wild megaherbivore grazing as a conservation tool to manage open habitats. We studied the effect of wild ungulates in a 160 ha game preserve in NE Germany in three successional stages (i) Corynephorus canescens-dominated grassland, (ii) ruderal tall forb vegetation dominated by Tanacetum vulgare and (iii) Pinus sylvestris-pioneer forest over three years. Our results demonstrate that wild megaherbivores considerably affected species composition and delayed successional pathways in open habitats. Grazing effects differed considerably between successional stages: species richness was higher in grazed ruderal and pioneer forest plots, but not in the Corynephorus sites. Species composition changed significantly in the Corynephorus and ruderal sites. Grazed ruderal sites had turned into sites with very short vegetation dominated by Agrostis spp. and the moss Brachythecium albicans, most species did not flower. Woody plant cover was significantly affected only in the pioneer forest sites. Young pine trees were severely damaged and tree height was considerably reduced, leading to a “Pinus-macchie”-appearance. Ecological patterns and processes are known to vary with spatial scale. Since grazing by megaherbivores has a strong spatial component, the scale of monitoring success of grazing may largely differ among and within different systems. Thus, the second aim of this thesis was to test whether grazing effects are consistent over different spatial scales, and to give recommendations for appropriate monitoring scales. For this purpose, we studied grazing effects on plant community structure using multi-scale plots that included three nested spatial scales (0.25 m2, 4 m2, and 40 m2). Over all vegetation types, the scale of observation directly affected grazing effects on woody plant cover and on floristic similarity, but not on the proportion of open soil and species richness. Grazing effects manifested at small scales regarding floristic similarity in pioneer forest and ruderal sites and regarding species richness in ruderal sites. The direction of scale-effects on similarity differed between vegetation types: Grazing effects on floristic similarity in the Corynephorus sites were significantly higher at the medium and large scale, while in the pioneer forest sites they were significantly higher at the smallest scale. Disturbances initiate vegetation changes by creating gaps and affecting colonization and extinction rates. The third intention of the thesis was to investigate the effect of small-scale disturbances on the species-level. In a sowing experiment, we studied early establishment probabilities of Corynephorus canescens, a key species of open sandy habitats. Applying two different regimes of mechanical ground disturbance (disturbed and undisturbed) in the three successional stages mentioned above, we focused on the interactive effects of small-scale disturbances, successional stage and year-to-year variation. Disturbance led to higher emergence in a humid and to lower emergence in a very dry year. Apparently, when soil moisture was sufficient, the main factor limiting C. canescens establishment was competition, while in the dry year water became the limiting factor. Survival rates were not affected by disturbance. In humid years, C. canescens emerged in higher numbers in open successional stages while in the dry year, emergence rates were higher in late stages, suggesting an important role of late successional stages for the persistence of C. canescens. We conclude that wild ungulate grazing is a useful tool to slow down succession and to preserve a species-rich, open landscape, because it does not only create disturbances, thereby supporting early successional stages, but at the same time efficiently controls woody plant cover. However, wild ungulate grazing considerably changed the overall appearance of the landscape. Additional measures like shifting exclosures might be necessary to allow vulnerable species to flower and reproduce. We further conclude that studying grazing impacts on a range of scales is crucial, since different parameters are affected at different spatial scales. Larger scales are suitable for assessing grazing impact on structural parameters like the proportion of open soil or woody plant cover, whereas species richness and floristic similarity are affected at smaller scales. Our results further indicate that the optimal strategy for promoting C. canescens is to apply disturbances just before seed dispersal and not during dry years. Further, at the landscape scale, facilitation by late successional species may be an important mechanism for the persistence of protected pioneer species.
Using a serial search paradigm, we observed several effects of within-object fixation position on spatial and temporal control of eye movements: the preferred viewing location, launch site effect, the optimal viewing position, and the inverted optimal viewing position of fixation duration. While these effects were first identified by eye-movement studies in reading, our approach permits an analysis of the functional relationships between the effects in a different paradigm. Our results demonstrate that the fixation position is an important predictor of the subsequent saccade by influencing both fixation duration and the selection of the next saccade target.
Extract: [...]Of all the print-media newspapers are the most commonly used. They are not literature in the sense of belles letters, but they should not be underestimated in their political, social and personal importance. No other printed product is as closely linked with everyday life as the newspapers. The day begins under their influence, and their contents mirror the events of the day with varying accuracy. Newspapers are strongly reader-oriented. They want to inform, but they also want to instil opinions. Specific choices of information shape the content level. Specific choices of language are resorted to in order to spread opinions and viewpoints. Language creates solidarity between the producers and the consumers of newspapers and thereby supports ideologies by specifically targeted linguistic means. Other strategies are employed for the same purpose, too. Visual aspects are of great importance, such as the typographical layout, the use of pictures, drawings, colours, fonts, etc.[...]
The overall program "arborescent numbers" is to similarly perform the constructions from the natural numbers (N) to the positive fractional numbers (Q+) to positive real numbers (R+) beginning with (specific) binary trees instead of natural numbers. N can be regarded as the associative binary trees. The binary trees B and the left-commutative binary trees P allow the hassle-free definition of arbitrary high arithmetic operations (hyper ... hyperpowers). To construct the division trees the algebraic structure "coppice" is introduced which is a group with an addition over which the multiplication is right-distributive. Q+ is the initial associative coppice. The present work accomplishes one step in the program "arborescent numbers". That is the construction of the arborescent equivalent(s) of the positive fractional numbers. These equivalents are the "division binary trees" and the "fractional trees". A representation with decidable word problem for each of them is given. The set of functions f:R1->R1 generated from identity by taking powers is isomorphic to P and can be embedded into a coppice by taking inverses.
This thesis addresses real-time rendering techniques for 3D information lenses based on the focus & context metaphor. It analyzes, conceives, implements, and reviews its applicability to objects and structures of virtual 3D city models. In contrast to digital terrain models, the application of focus & context visualization to virtual 3D city models is barely researched. However, the purposeful visualization of contextual data of is extreme importance for the interactive exploration and analysis of this field. Programmable hardware enables the implementation of new lens techniques, that allow the augmentation of the perceptive and cognitive quality of the visualization compared to classical perspective projections. A set of 3D information lenses is integrated into a 3D scene-graph system: • Occlusion lenses modify the appearance of virtual 3D city model objects to resolve their occlusion and consequently facilitate the navigation. • Best-view lenses display city model objects in a priority-based manner and mediate their meta information. Thus, they support exploration and navigation of virtual 3D city models. • Color and deformation lenses modify the appearance and geometry of 3D city models to facilitate their perception. The presented techniques for 3D information lenses and their application to virtual 3D city models clarify their potential for interactive visualization and form a base for further development.
While the Information Structure (IS) is most naturally interpreted as 'structure of information', some may argue that it is structure of something else, and others may object to the use of the word 'structure'. This paper focuses on the question of whether the informational component can have structural properties such that it can be called 'structure'. The preliminary conclusion is that, although there are some vague indications of structurehood in it, it is perhaps better understood to be a representation that encodes a finite set of information-based partitions, rather than structure.
The most recent trend in the studies of LF intervention effects makes crucial reference to focusing effects on the interveners, and this paper critically examines the representative analyses of the focus-based approach. While each analysis has its own merits and shortcomings, I argue that a pragmatic analysis that does not make appeal to syntactic configurations is better equipped to deal with many of the complex and delicate facts surrounding intervention effects.
While there is strong evidence for clumping in the winds of massive hot stars, very little is known about clumping in the winds from Central Stars. We have checked [WC]-type CSPN winds for clumping by inspecting the electron-scattering line wings. At least for three stars we found indications for wind inhomogeneities.
Spring algal development in deep temperate lakes is thought to be strongly influenced by surface irradiance, vertical mixing and temperature, all of which are expected to be altered by climate change. Based on long-term data from Lake Constance, we investigated the individual and combined effects of these variables on algal dynamics using descriptive statistics, multiple regression models and a processoriented dynamic simulation model. The latter considered edible and less-edible algae and was forced by observed or anticipated irradiance, temperature and vertical mixing intensity. Unexpectedly, irradiance often dominated algal net growth rather than vertical mixing for the following reason: algal dynamics depended on algal net losses from the euphotic layer to larger depth due to vertical mixing. These losses strongly depended on the vertical algal gradient which, in turn, was determined by the mixing intensity during the previous days, thereby introducing a memory effect. This observation implied that during intense mixing that had already reduced the vertical algal gradient, net losses due to mixing were small. Consequently, even in deep Lake Constance, the reduction in primary production due to low light was often more influential than the net losses due to mixing. In the regression model, the dynamics of small, fast-growing algae was best explained by vertical mixing intensity and global irradiance, whereas those of larger algae were best explained by their biomass 1 week earlier. The simulation model additionally revealed that even in late winter grazing may represent an important loss factor during calm periods when losses due to mixing are small. The importance of losses by mixing and grazing changed rapidly as it depended on the variable mixing intensity. Higher temperature, lower global irradiance and enhanced mixing generated lower algal biomass and primary production in the dynamic simulation model. This suggests that potential consequences of climate change may partly counteract each other.
Regulation of planktonic ciliate dynamics and functional composition during spring in Lake Constance
(2007)
Protozoans are among the most important grazers of phytoplankton and remineralizers of nutrients in marine and freshwater ecosystems, but less is known about the regulation of their population dynamics. We analyzed a 12 yr data set of ciliate biomass and species composition in large, deep Lake Constance to understand the factors influencing ciliate spring development. The start of ciliate net growth in spring was closely linked to that of edible algae, chlorophyll a and the vertical mixing intensity, but independent of water temperature. During ciliate spring growth, the relative contribution of ciliated interception feeders was positively related to that of cryptomonads, whereas the relative contribution of filter feeders correlated positively with that of non-cryptomonads. The duration of ciliate dominance in spring was largely controlled by the highly variable onset of the phytoplankton bloom, as the termination of the ciliate bloom was less variable. During years with an extended spring bloom of algae and ciliates, internally forced species shifts were observed in both communities. Interception feeders alternated with filter feeders in their relative importance as did cryptomonads and non-cryptomonads. Extended spring blooms were observed when vertical mixing intensity was low at low temperatures during early spring, which will become less likely under the anticipated climate change scenarios. The termination of the ciliate spring bloom occurred prior to a reduction in food concentration and mostly also prior to the mass development of daphnids alone, but coincided with increased grazing by various predators together, such as rotifers, copepods and daphnids in late May/early June.
We present projects for future space missions using new quantum devices based on ultracold atoms. They will enable fundamental physics experiments testing quantum physics, physics beyond the standard model of fundamental particles and interactions, special relativity, gravitation and general relativity.
Extract: [...]The New English Dictionary, later to become the Oxford English Dictionary, was first published between 1884 and 1928. To add new material, two supplements were issued after this, the first in 1933, and another, more extensive one between 1972 and 1986. In 1989, the Oxford English Dictionary, second edition (OED2) was published, which integrated the material from the original dictionary and the supplements into a single alphabetical sequence. However, virtually all material contained in this edition still remained in the form in which it was originally published. This is the edition most commonly used today, as it forms the basis of the Oxford English Dictionary Online and is also still being sold in print and on CD-ROM. In 1991, a new project started to revise the entire dictionary and bring its entries up to date, both in terms of English usage and in terms of associated scholarship, such as encyclopaedic information and etymologies. The scope was also widened, placing a greater emphasis on English spoken outside Britain. The revision of the dictionary began with the letter M, and the first updated entries were published online in March 2000 (OED3). Quarterly publication of further material has extended the range of revised entries as far as PROTEOSE n. (June 2007). New words from all parts of the alphabet have been published alongside the regular revision.[...]
Magnetorotational instability (MRI) is one of the most important and most common instabilities in astrophysics. Today it is widely accepted that it serves as a major source of turbulent viscosity in accretion disks, the most energy efficient objects in the universe. The importance of the MRI for astrophysics has been realized only in recent fifteen years. However, originally it was discovered much earlier, in 1959, in a very different context. Theoretical flow of a conducting liquid confined between differentially rotating cylinders in the presence of an external magnetic field was analyzed. The central conclusion is that the additional magnetic field parallel to the axis of rotation can destabilize otherwise stable flow. Theory of non-magnetized fluid motion between rotating cylinders has much longer history, though. It has been studied already in 1888 and today such setup is usually referred as a Taylor-Couette flow. To prove experimentally the existence of MRI in a magnetized Taylor-Couette flow is a demanding task and different MHD groups around the world try to achieve it. The main problem lies in the fact that laboratory liquid metals which are used in such experiments are characterized by small magnetic Prandtl number. Consequently rotation rates of the cylinders must be extremely large and vast amount of technical problems emerge. One of the most important difficulties is an influence of plates enclosing the cylinders in any experiment. For fast rotation the plates tend to dominate the whole flow and the MRI can not be observed. In this thesis we discuss a special helical configuration of the applied magnetic field which allows the critical rotation rates to be much smaller. If only the axial magnetic field is present, the cylinders must rotate with angular velocities corresponding to Reynolds numbers of order Re ≈ 10^6. With the helical field this number is dramatically reduced to Re ≈ 10^3. The azimuthal component of the magnetic field can be easily generated by letting an electric current through the axis of rotation, In a Taylor-Couette flow the (primary) instability manifests itself as Taylor vortices. The specific geometry of the helical magnetic field leads to a traveling wave solution and the vortices are drifting in a direction determined by rotation and the magnetic field. In an idealized study for infinitely long cylinders this is not a problem. However, if the cylinders have finite length and are bounded vertically by the plates the situation is different. In this dissertation it is shown, with use of numerical methods, that the traveling wave solution also exists for MHD Taylor-Couette flow at finite aspect ratio H/D, H being height of the cylinders, D width of the gap between them. The nonlinear simulations provide amplitudes of fluid velocity which are helpful in designing an experiment. Although the plates disturb the flow, parameters like the drift velocity indicate that the helical MRI operates in this case. The idea of the helical MRI was implemented in a very recent experiment PROMISE. The results provided, for the first time, an evidence that the (helical) MRI indeed exists. Nevertheless, the influence of the vertical endplates was evident and the experiment can be, in principle, improved. Exemplary methods of reduction of the end-effect are here proposed. Near the vertical boundaries develops an Ekman-Hartmann layer. Study of this layer for the MHD Taylor-Couette system as well as its impact on the global flow properties is presented. It is shown that the plates, especially if they are conducting, can disturb the flow far more then previously thought also for relatively slow rotation rates.
Luminous Blue Variables show strong changes in their stellar wind on time scales of typically years to decades when they expand and contract radially at approximately constant luminosity. Micro-variability on shorter time scales and amplitudes can be observed superimposed to the larger scale radial changes. I will show long-term time series of high resolution spectra which we have collected in the past 20 years for many of the well known LBVs together with a few time series of weekly sampling (HR Car, R40, R71, R110, R127, S Dor) covering a time windows of up to a few months. Wind variability is seen on short and intermediate time scales with the line profiles changing from P Cygni to inverse P Cygni and double peeked profiles sometimes for the same star and spectral line. On longer time scales the ionisation levels for all chemical elements change drastically due to the strong change of the temperature on the stellar surface. While on the long term the characteristic radial changes may have impact on the over all mass loss rates, the variabilities and asymmetries on short and intermediate time scales may cause false estimates of the mass loss rates when confronting models with the observed line profiles
The solar tachocline is a thin transition layer between the solar radiative zone rotating uniformly and the solar convection zone, which has a mainly latitudinal differential rotation profile. This layer has a thickness of less than $0.05R_{\sun}$ and is subject to extreme radial as well as latitudinal shears. Helioseismological estimates put this layer at roughly $0.7R_{\sun}$. The tachocline mostly resides in the sub-adiabatic, non-turbulent radiative interior, except for a small overlap with the convection zone on the top. Many proposed dynamo mechanisms involve strong toroidal magnetic fields in this transition region. The exact mechanisms behind the formation of such a thin layer is still disputed. A very plausible mechanism is the one involving a weak, relic poloidal magnetic field trapped inside the radiative zone, which is responsible for expelling differential rotation outwards. This was first proposed by \citet{RK97}. The present work develops this idea with numerical simulations including additional effects like meridional circulation. It is shown that a relic field of 1~Gauss or smaller would be sufficient to explain the observed thickness of the tachocline. The stability of the solar tachocline is addressed as the next part of the problem. It is shown that the tachocline is stable up to a differential rotation of 52\% in the absence of magnetic fields. This is a new finding as compared to the earlier two dimensional models which estimated the solar differential rotation (about 28\%) to be marginally stable or even unstable. The changed stability limit is attributed to the changed stability criterion of the 3-dimensional model which also involves radial gradients of the angular velocity. In the presence of toroidal magnetic field belts, the lowest non-axisymmetric mode is shown to be the most unstable one for the radiative part of the tachocline. It is estimated that the tachocline would become unstable for toroidal fields exceeding about 100~Gauss. With both formation and stability questions satisfactorily addressed, this work presents the most comprehensive analysis of the physical processes in the solar tachocline to date.
Extract: [...]The Roman conquest of what was to become the province of Gallia Narbonensis in the second and then of the whole of Transalpine Gaul in the first century B.C. led to the incorporation into the Roman empire of a large part of the territory in which Gaulish was then spoken.1 In consequence, the vernacular rapidly lost its footing at least in public life and was soon replaced by Latin, the language of the new masters, which enjoyed higher prestige (cf. e.g. Meid 1980: 7-8). On the other hand, Gaulish continued to be written for some three centuries and was probably used in speech even longer, especially in rural areas. We must therefore posit a prolonged period of bilingualism. The effects of this situation on the Latin spoken in the provinces of Gaul seem to have been rather limited. A number of lexical items, mostly from the field of everyday life, and some phonetic characteristics are the sole testimonies of a Gaulish substratum in the variety of Latin that was later to develop into the Romance dialects of France (cf. Meid 1980: 38, fn. 77). [...]
The requirements of modern e-learning techniques change. Aspects such as community interaction, flexibility, pervasive learning and increasing mobility in communication habits become more important. To meet thesechallenges e-learning platforms must provide support on mobile learning. Most approaches try to adopt centralized and static elearning mechanisms to mobile devices. However, often technically it is not possible for all kinds of devices to be connected to a central server. Therefore we introduce an application of a mobile e-learning network which operates totally decentralized with the help of an underlying ad hoc network architecture. Furthermore the concept of ad hoc messaging network (AMNET) is used as basis system architecture for our approach to implement a platform for pervasive mobile elearning.
The requirements of modern e-learning techniques change. Aspects such as community interaction, flexibility, pervasive learning and increasing mobility in communication habits become more important. To meet these challenges e-learning platforms must provide support on mobile learning. Most approaches try to adopt centralised and static e-learning mechanisms to mobile devices. However, often technically it is not possible for all kinds of devices to be connected to a central server. Therefore we introduce an application of a mobile e-learning network which operates totally decentralised with the help of an underlying ad hoc network architecture. Furthermore the concept of ad hoc messaging network (AMNET) is used as basis system architecture for our approach to implement a platform for pervasive mobile e-learning.
Aim The aim of the present study was to examine young female volleyballers’ body build, physical abilities, technical skills and psychophysiological properties in relation to their performance at competitions. The sample consisted of 46 female volleyballers aged 13-16 years. 49 basic anthropometric measurements were measured and 65 proportions and body composition characteristics were calculated. 9 physical ability tests, 9 volleyball technical skills tests and 21 psychophysiological tests were carried out. The game performance was recorded by the computer program Game. The program enabled to fix the performance of technical elements in case of each player. The computer program Game calculated the index of proficiency in case of each girl for each element. The first control group consisted of 74 female volleyballers aged 13–15 years with whom reduced anthropometry was provided and 28 games were recorded. The second control group consisted of 586 ordinary schoolgirls aged 13–16 years with whom full anthropometry was provided. Results In order to systematize all anthropometric characteristics, we first studied the essence of the anthropometric structure of the body as a whole. It turned out to be a characteristic system where all variables are in significant correlation between one another and where the leading characteristics are height and weight. Therefore we based the classification on the mean height and weight of the whole sample. We formed a 5 class SD classification. There are three classes of concordance between height and weight: small height – small weight, medium height – medium height, big height – big weight. The other two classes were classes of disconcordance between height and weight- pycnomorphs and leptomorphs. We managed to show that gradual increase in height and weight brought about statistically significant increase in length, breadth and depth measurements, circumferences, bone thicknesses and skinfolds. There were also systematic changes in indeces and body composition characteristics. Pycnomorphs and leptomorphs also showed differences specific to their body types in body measurements and body composition. The results of all tests were submitted to basic statistical analysis and all correlations were found between all the tests (volleyball technical skills, psychophysiological abilities, physical abilities), and all basic anthropometric variables (n = 49) and all proportions and body composition characteristics (n = 65). All anthropometric measurements and test results were correlated with the index of proficiency for all elements of the game. The best linear regression models were calculated for predicting proficiency in different elements of the game. We can see that body build and all kind of tests took part in predicting the proficiency of the game. The most essential for performing attack, block and feint were anthropometric and psychophysiological models. The studied complex of body build characteristics and tests results determine the players’ proficiency at competitions, are an important tool for testing the player’s individual development, enable to choose volleyballers from among schoolgirls and represent the whole body constitutional model of a young female volleyballer. Outlook Our outlook for the future is to continue recording of all Estonian championship games with the computer program Game, to continue the players’ anthropometric measuring and psychophysiological testing at competitions and to compile a national register for assessment of development of individual players and teams.
Extract: [...] One of the often noted characteristic features of the Celtic languages is the absence of a singular verbal form with the meaning ‘to have’.1 The principal way of expressing possession is through periphrastic constructions with prepositions (such as Irish ag, Scottish Gaelic aig ‘at’; Welsh gan, Breton gant ‘at, with’) and appropriate forms of the substantive verb. Pronominal prepositions, another distinctive feature of the Celtic languages, consist of a preposition and a suffixed pronoun, or rather a pronominal personal ending. This construction may be analyzed as an instance of category fusion. Thus, the Irish and Welsh equivalents of English ‘I have money’ are Tá airgead agam or Mae arian gen i, respectively, both literally meaning ‘is money at-me/with-me’. This note discusses pronominal possessive constructions in Celtic languages (and some comparable examples from Celtic Englishes) and provides some background information on pronominal prepositions and comments on historical developments of these forms. It also discusses some terminological issues involved in labelling the construction in question. [...]
We report on new mass-loss rate estimates for O stars in six massive binaries using the amplitude of orbital-phase dependent, linear-polarimetric variability caused by electron scattering off free electrons in the winds. Our estimated mass-loss rates for luminous O stars are independent of clumping. They suggest similar clumping corrections as for WR stars and do not support the recently proposed reduction in mass-loss rates of O stars by one or two orders of magnitude.
Chlamydomonas acidophila, a dominant phytoplankton species in the very acidic Lake 111 (pH 2.7) situated in Germany, faces low concentrations of inorganic phosphorus (P-i), inorganic carbon (C-i) and potassium (K+) in its environment, which may lead to a complex colimitation by these nutrients. We performed laboratory and field investigations to test for P-i limitation and its dependence on C-i and K+ concentrations. The minimum cell quota for phosphorus (Q(0)) and phosphatase enzyme activity were similar to those for neutrophilic algae, despite the low pH and high concentrations of iron and aluminium, indicating no extra metabolic costs or inhibition of enzymes by the extreme environment. The threshold concentration of soluble reactive phosphorus for growth (SRPt), the algal C:P ratio and the alkaline phosphatase enzyme activity all suggested a moderate P-i limitation of C. acidophila in Lake 111. SRPt and Q(0) were higher at low CO2 and K+ concentrations in culture, showing a relationship between C-i and P-i acquisition. Furthermore, SRPt and Q(0) were higher under K+/P-i-colimiting conditions than under P-i-limiting conditions alone, suggesting that K+ concentrations influence P-i limitation in C. acidophila as well. Our results show that a limitation by one macronutrient requires consideration of the availability of the others as their uptake mechanisms depend on each other. Notwithstanding these interactions, C-i or K+ concentrations had no clear influence on the P-i limitation of C. acidophila in Lake 111.
Chlamydomonas acidophila faces high heavy-metal concentrations in acidic mining lakes, where it is a dominant phytoplankton species. To investigate the importance of metals to C. acidophila in these lakes, we examined the response of growth, photosynthesis, cell structure, heat-shock protein (Hsp) accumulation, and metal adsorption after incubation in metal-rich lake water and artificial growth medium enriched with metals (Fe, Zn). Incubation in both metal-rich lake water and medium caused large decreases in photosystem II function (though no differences among lakes), but no decrease in growth rate (except for medium + Fe). Concentrations of small Hsps were higher in algae incubated in metal-rich lake- water than in metal-enriched medium, whereas Hsp60 and Hsp70A were either less or equally expressed. Cellular Zn and Fe contents were lower, and metals adsorbed to the cell surface were higher, in lake-water-incubated algae than in medium- grown cells. The results indicate that high Zn or Fe levels are likely not the main or only contributor to the low primary production in mining lakes, and multiple adaptations of C. acidophila (e.g., high Hsp levels, decreased metal accumulation) increase its tolerance to metals and permit survival under such adverse environmental conditions. Supposedly, the main stress factor present in the lake water is an interaction between low P and high Fe concentrations.
The James Webb Space Telescope (JWST) is a large, infrared-optimized space telescope scheduled for launch in 2013. JWST will find the first stars and galaxies that formed in the early universe, connecting the Big Bang to our own Milky Way galaxy. JWST will peer through dusty clouds to see stars forming planetary systems, connecting the MilkyWay to our own Solar System. JWST’s instruments are designed to work primarily in the infrared range of 1 - 28 μm, with some capability in the visible range. JWST will have a large mirror, 6.5 m in diameter, and will be diffraction-limited at 2 μm (0.1 arcsec resolution). JWST will be placed in an L2 orbit about 1.5 million km from the Earth. The instruments will provide imaging, coronography, and multi-object and integral-field spectroscopy across the 1 - 28 μm wavelength range. The breakthrough capabilities of JWST will enable new studies of massive star winds from the Milky Way to the early universe.
I discuss observational evidence – independent of the direct spectral diagnostics of stellar winds themselves – suggesting that mass-loss rates for O stars need to be revised downward by roughly a factor of three or more, in line with recent observed mass-loss rates for clumped winds. These independent constraints include the large observed mass-loss rates in LBV eruptions, the large masses of evolved massive stars like LBVs and WNH stars, WR stars in lower metallicity environments, observed rotation rates of massive stars at different metallicity, supernovae that seem to defy expectations of high mass-loss rates in stellar evolution, and other clues. I pay particular attention to the role of feedback that would result from higher mass-loss rates, driving the star to the Eddington limit too soon, and therefore making higher rates appear highly implausible. Some of these arguments by themselves may have more than one interpretation, but together they paint a consistent picture that steady line-driven winds of O-type stars have lower mass-loss rates and are significantly clumped.
Functional analysis of selected DOF transcription factors in the model plant Arabidopsis thaliana
(2007)
Transcription factors (TFs) are global regulators of gene expression playing essential roles in almost all biological processes, and are therefore of great scientific and biotechnological interest. This project focused on functional characterisation of three DNA-binding-with-one-zinc-finger (DOF) TFs from the genetic model plant Arabidopsis thaliana, namely OBP1, OBP2 and AtDOF4;2. These genes were selected due to severe growth phenotypes conferred upon their constitutive over-expression. To identify biological processes regulated by OBP1, OBP2 and AtDOF4;2 in detail molecular and physiological characterization of transgenic plants with modified levels of OBP1, OBP2 and AtDOF4;2 expression (constitutive and inducible over-expression, RNAi) was performed using both targeted and profiling technologies. Additionally expression patterns of studied TFs and their target genes were analyzed using promoter-GUS lines and publicly available microarray data. Finally selected target genes were confirmed by chromatin immuno-precipitation and electrophoretic-mobility shift assays. This combinatorial approach revealed distinct biological functions of OBP1, OBP2 and AtDOF4;2. Specifically OBP2 controls indole glucosinolate / auxin homeostasis by directly regulating the enzyme at the branch of these pathways; CYP83B1 (Skirycz et al., 2006). Glucosinolates are secondary compounds important for defence against herbivores and pathogens in the plants order Caparales (e.g. Arabidopsis, canola and broccoli) whilst auxin is an essential plant hormone. Hence OBP2 is important for both response to biotic stress and plant growth. Similarly to OBP2 also AtDOF4;2 is involved in the regulation of plant secondary metabolism and affects production of various phenylpropanoid compounds in a tissue and environmental specific manner. It was found that under certain stress conditions AtDOF4;2 negatively regulates flavonoid biosynthetic genes whilst in certain tissues it activates hydroxycinnamic acid production. It was hypothesized that this dual function is most likely related to specific interactions with other proteins; perhaps other TFs (Skirycz et al., 2007). Finally OBP1 regulates both cell proliferation and cell expansion. It was shown that OBP1 controls cell cycle activity by directly targeting the expression of core cell cycle genes (CYCD3;3 and KRP7), other TFs and components of the replication machinery. Evidence for OBP1 mediated activation of cell cycle during embryogenesis and germination will be presented. Additionally and independently on its effects on cell proliferation OBP1 negatively affects cell expansion via reduced expression of cell wall loosening enzymes. Summing up this work provides an important input into our knowledge on DOF TFs function. Future work will concentrate on establishing exact regulatory networks of OBP1, OBP2 and AtDOF4;2 and their possible biotechnological applications.
Formal poincare lemma
(2007)
Formal Poincaré lemma
(2007)
We show how the multiple application of the formal Cauchy-Kovalevskaya theorem leads to the main result of the formal theory of overdetermined systems of partial differential equations. Namely, any sufficiently regular system Au = f with smooth coefficients on an open set U ⊂ Rn admits a solution in smooth sections of a bundle of formal power series, provided that f satisfies a compatibility condition in U.
Multinuclear dynamic NMR spectroscopy of 3,5-bis(trifluoromethylsulfonyl)-1,3,5-oxadiazinane (3) revealed the existence of two conformers with differently oriented CF3 groups with respect to the ring, and two dynamic processes: ring inversion and restricted rotation about the N-S bond. Two transition states connecting the two conformers and corresponding to clockwise and counterclockwise rotations about the N-S bond were found; the calculated activation barriers of about 12 kcal/mol are in excellent agreement with those measured experimentally for the related molecule 1,3,5-tris(trifluoromethylsulfonyl)-1,3,5-triazinane (1). X-ray analysis proved the existence of the symmetric isomer of 3, which is the minor isomer in solutions but the only one in the crystal due to packing effects. The normal Perlin effect (JCHax < JCHeq)observed for 2(6)-CH2 in 3, whereas the reversed Perlin effect was found for the 4-CH2 group in 3 as well as for all CH2 groups in 1 both experimentally and theoretically. The latter effect in compounds 1, 3, and 1- (methylsulfonyl)-3,5-bis(trifluoromethylsulfonyl)-1,3,5-triazinane (2) can be considered as a genuine reverse Perlin effect since larger values of 1JCH are observed for longer C-H bonds.
Lists as embedded structures and the prosody of list construction as an interactional resource
(2007)
After giving an overview of the treatment of lists in the literature, I describe lists in German talk-in- interaction. I show that, apart from the preference for three-part lists described by Jefferson (1990), lists are embedded in a larger three-component structure that the list is the middle part of. For lists proper, I suggest to differentiate between closed and open lists that are produced with different kinds of practices. It is the prosody that is used to suggest the list as made up of a closed or an open number of list items, irrespective of its syntactic embedding. I then concentrate on open lists, in particular their intonation. Open lists may be produced with different kinds of, albeit similar, intonation contours. But it is not so much the particular intonation contour that is constitutive of lists, but a variety of similar contours plus the repetition of the chosen contour for at least some or even all of the list items. Furthermore, intonation is deployed to suggest the interpretation of a potential final list item as either a designed list completer or as another designed item of the list. The design of this final list item as a completer or as another list item is used as a practice to signal the non-completion or completion of the list proper. But even after completing the list proper, the larger three-component structure also has to be closed in order to embed and accommodate the list into the surrounding sequential interaction. For the analysis of the practices of list construction I am concentrating on the role of prosody, especially intonation, giving evidence to show that intonation is indeed one of the methodically used constitutive cues that makes the production and structuring of lists recognizable for recipients.
Cloning and characterization of a single chain antibody to glucose oxidase from a murine hybridoma
(2007)
Glucose oxidase (GOD) is an oxidoreductase catalyzing the reaction of glucose and oxygen to peroxide and gluconolacton (EC 1.1.3.4.). GOD is a widely used enzyme in biotechnology. Therefore the production of monoclonal antibodies and antibody fragments to GOD are of interest in bioanalytics and even tumor therapy. We describe here the generation of a panel of monoclonal antibodies to native and heat inactivated GOD. One of the hybridomas, E13BC8, was used for cloning of a single chain antibody (scFv). This scFv was expressed in Escherichia coli XL1-blue with the help of the vector system pOPE101. The scFv was isolated from the periplasmic fraction and detected by western blotting. It reacts specifically with soluble active GOD but does not recognize denatured GOD adsorbed to the solid phase. The same binding properties were also found for the monoclonal antibody E13BC8.
New evidence is provided for a grammatical principle that singles out contrastive focus (Rooth 1996; Truckenbrodt 1995) and distinguishes it from discourse-new “informational” focus. Since the prosody of discourse-given constituents may also be distinguished from discourse-new, a three-way distinction in representation is motivated. It is assumed that an F-feature marks just contrastive focus (Jackendoff 1972, Rooth 1992), and that a G-feature marks discoursegiven constituents (Féry and Samek-Lodovici 2006), while discoursenew is unmarked. A crucial argument for G-marking comes from second occurrence focus (SOF) prosody, which arguably derives from a syntactic representation where SOF is both F-marked and G-marked. This analysis relies on a new G-Marking Condition specifying that a contrastive focus may be G-marked only if the focus semantic value of its scope is discourse-given, i.e. only if the contrast itself is given.