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Deep crustal structure of the Northeast German Basin : new DEKORP-BASIN ï96 deep-profiling results
(1999)
Prostaglandin E(2) receptors (EP-Rs) belong to the family of heterotrimeric G protein-coupled ectoreceptors with seven transmembrane domains. They can be subdivided into four subtypes according to their ligand-binding and G protein-coupling specificity: EP1 couple to G(q), EP2 and EP4 to G(s), and EP3 to G(i). The EP4-R, in contrast to the EP3beta-R, shows rapid agonist-induced desensitization. The agonist-induced desensitization depends on the presence of the EP4-R carboxyl-terminal domain, which also confers desensitization in a G(i)-coupled rEP3hEP4 carboxyl-terminal domain receptor hybrid (rEP3hEP4-Ct-R). To elucidate the possible mechanism of this desensitization, in vivo phosphorylation stimulated by activators of second messenger kinases, by prostaglandin E(2), or by the EP3-R agonist M&B28767 was investigated in COS-7 cells expressing FLAG-epitope-tagged rat EP3beta-R (rEP3beta-R), hEP4-R, or rEP3hEP4- Ct-R. Stimulation of protein kinase C with phorbol-12-myristate-13-acetate led to a slight phosphorylation of the FLAG- rEP3beta-R but to a strong phosphorylation of the FLAG-hEP4-R and the FLAG-rEP3hEP4-Ct-R, which was suppressed by the protein kinase A and protein kinase C inhibitor staurosporine. Prostaglandin E(2) stimulated phosphorylation of the FLAG- hEP4-R in its carboxyl-terminal receptor domain. The EP3-R agonist M&B28767 induced a time- and dose-dependent phosphorylation of the FLAG-rEP3hEP4-Ct-R but not of the FLAG-rEP3beta-R. Agonist-induced phosphorylation of the FLAG- hEP4-R and the FLAG-rEP3hEP4-Ct-R were not inhibited by staurosporine, which implies a role of G protein-coupled receptor kinases (GRKs) in agonist-induced receptor phosphorylation. Overexpression of GRKs in FLAG-rEP3hEP4-Ct-R- expressing COS-7 cells augmented the M&B28767-induced receptor phosphorylation and receptor sequestration. These findings indicate that phosphorylation of the carboxyl-terminal hEP4-R domain possibly by GRKs but not by second messenger kinases may be involved in rapid agonist-induced desensitization of the hEP4-R and the rEP3hEP4-Ct-R.
For the five principal prostanoids PGD2, PGE2, PGF2alpha, prostacyclin and thromboxane A2 eight receptors have been identified that belong to the family of G-protein-coupled receptors. They display an overall homology of merely 30%. However, single amino acids in the transmembrane domains such as an Arg in the seventh transmembrane domain are highly conserved. This Arg has been identified as part of the ligand binding pocket. It interacts with the carboxyl group of the prostanoid. The aim of the current study was to analyze the potential role in ligand binding of His-81 in the second transmembrane domain of the rat PGF2alpha receptor, which is conserved among all PGF2alpha receptors from different species. Molecular modeling suggested that this residue is located in close proximity to the ligand binding pocket Arg 291 in the 7th transmembrane domain. The His81 (H) was exchanged by site-directed mutagenesis to Gln (Q), Asp (D), Arg (R), Ala (A) and Gly (G). The receptor molecules were N-terminally extended by a Flag epitope for immunological detection. All mutant proteins were expressed at levels between 50% and 80% of the wild type construct. The H81Q and H81D receptor bound PGF2alpha with 2-fold and 25-fold lower affinity, respectively, than the wild type receptor. Membranes of cells expressing the H81R, H81A or H81G mutants did not bind significant amounts of PGF2alpha. Wild type receptor and H81Q showed a shallow pH optimum for PGF2alpha binding around pH 5.5 with almost no reduction of binding at higher pH. In contrast the H81D mutant bound PGF2alpha with a sharp optimum at pH 4.5, a pH at which the Asp side chain is partially undissociated and may serve as a hydrogen bond donor as do His and Gln at higher pH values. The data indicate that the His-81 in the second transmembrane domain of the PGF2alpha receptor in concert with Arg-291 in the seventh transmembrane domain may be involved in ligand binding, most likely not by ionic interaction with the prostaglandin's carboxyl group but rather as a hydrogen bond donor.
BACKGROUND/AIMS: Prostanoids produced by nonparenchymal cells modulate the function of parenchymal and nonparenchymal liver cells during homeostasis and inflammation via eight classes of prostanoid receptors coupled to different G-proteins. Prostanoid receptor expression in parenchymal and nonparenchymal cells was studied in order to get a better insight into the complex prostanoid-mediated intrahepatic signaling network. METHODS: RNA was isolated from freshly purified parenchymal and nonparenchymal rat liver cells and the mRNA level of all eight prostanoid receptor classes was determined by newly developed semiquantitative reverse transcription-polymerase chain reaction protocols. RESULTS: The mRNAs for the prostanoid receptors were differentially expressed. Hepatocytes were the only cell type which contained the mRNA of the Gq-linked prostaglandin F2alpha receptor; they were devoid of any mRNA for the Gs-linked prostanoid receptors. Kupffer cells possessed the largest amount of mRNA for the Gs-linked prostaglandin E2 receptor subtype 2. Endothelial cells expressed high levels of mRNA for the Gq-linked thromboxane receptor and medium levels of mRNA for the Gs-linked prostacyclin receptor, while stellate cells had the highest levels of mRNA for the prostacyclin receptor. The mRNAs for the Gq-linked prostaglandin E2 receptor subtype 1 and the Gi-linked prostaglandin E2 receptor subtype 3 were expressed in hepatocytes and all nonparenchymal cell types at similar high levels, whereas the mRNA of the Gs-linked prostaglandin D2 receptor was expressed in all nonparenchymal cells at very low levels. CONCLUSIONS: In hepatocytes the prostaglandin F2alpha receptor can mediate an increase in glucose output via an increase of intracellular InsP3 while cAMP-dependent glucose output can be inhibited via the subtype 3 prostaglandin E2 receptor. The subtype 2 prostaglandin E2 receptor can restrain the inflammatory response of Kupffer cells via an increase in intracellular cAMP The thromboxane receptor and the prostacyclin receptor in sinusoidal endothelial and the prostacyclin receptor in stellate cells may be involved in the regulation of sinusoidal blood flow and filtration.
In the perfused rat liver the anaphylatoxin C5a enhanced glucose output, reduced flow, and elevated prostanoid overflow. Because hepatocytes (HCs) do not express C5a receptors, the metabolic C5a actions must be indirect, mediated by e.g. prostanoids from Kupffer cells (KCs) and hepatic stellate cells (HSCs), which possess C5a receptors. Surprisingly, the metabolic C5a effects were not only impaired by the prostanoid synthesis inhibitor, indomethacin, but also by the thromboxane A(2) (TXA(2)) receptor antagonist, daltroban, even though HCs do not express TXA(2) receptors. TXA(2) did not induce prostaglandin (PG) or an unknown factor release from KCs or sinusoidal endothelial cells (SECs), which express TXA(2) receptors, because (1) daltroban did neither influence the C5a-induced release of prostanoids from cultured KCs nor the C5a-dependent activation of glycogen phosphorylase in KC/HC cocultures and because (2) the TXA(2) analog, U46619, failed to stimulate prostanoid release from cultured KCs or SECs or to activate glycogen phosphorylase in KC/HC or SEC/HC cocultures. In the perfused liver, Ca(2+)-deprivation inhibited not only flow reduction but also glucose output elicited by C5a to similar extents as daltroban. Similarly, in the absence of extracellular Ca(2+), flow reduction and glucose output induced by U46619 were almost completely prevented, whereas glucose output induced by the directly acting PGF(2alpha) was only slightly lowered. Thus, in the perfused rat liver PGs released after C5a- stimulation from KCs and HSCs directly activated glycogen phosphorylase in HCs, and TXA(2) enhanced glucose output indirectly mainly by causing hypoxia as a result of flow reduction.
Local Orders, Global Chaos
(1999)
We study a model describing a rotating linear rigid molicule interacting with a Bose-Einstein condensate. A generalization of the Landau criterion is established and gives the limit for which the molecule moves translationally and rotationally into the condensate without any friction. In particular, we show that the rotational energy released by the molecule is large enough to eject one atom out of the condensate. The detection of such an atom provides a direct measurement of the low-energy cross section of the scattering with the rotating molecule. Finally, increases of inertia and of centrifugal distortion of the molecule due to the surrounding condensate are estimated and compared with the experimental data obtained for a He4 droplet.
We consider the role of weak interaction on the fluctuations of the number of condensed atoms within canonical and microanonical ensembles. Unlike the correspinding case of the ideal gas this is not a clean, well-defined problem of mathematical physics. Two related reasons are the following: there is no unique way of defining the condensate fraction of the interacting system and no exact energy levels of the interacting system are known.
We investigate the scattering of slowly moving atoms on the Bose-Einstein condensate. The condensate excitations are described by Bogolyubov-de Gennes equatins. We derive the analytic expressions for the differential cross section for both elastic and ineladtic channels. For the elastic channel we obtain analytic results for total cross sections, and discuss their scaling with the number of condensed atoms. For inelastic channels we present numerical results for the total cross section.
We derive exact thermodynamic identities relating the average number of condensed atoms and the root-mean- square fluctuations determined in different statistical ensembles for the weakly interacting Bose gas confined in a box. This is achieved by introducing the concept of auxiliary partition functions for model Hamiltonians that do conserve the total number of particles. Exploiting such thermodynamic identities, we provide the first, completely analytical prediction of the microcanonical particle number fluctuations in the weakly interacting Bose gas. Such fluctuations, as a function of the volume V of the box are found to behave normally, in contrast wiht the anomalous scaling behaviour V3/ 4 of the fluctuations in the ideal Bose gas.
We investigate the quantization of nonzero sum games. For the particular case of the Prisoners' Dilemma we show that this game ceases to pose a dilemma if quantum strategies are allowed for. We also construct a particular quantum strategy which always gives reward if played against any classical strategy.
We study the electromagnetic coupling and concomitant heating of a particle in a miniaturized trap close to a solid surface. Two dominant heating mechanisms are identified: proximity fields generated by thermally exicted currents in the absorbing solid and timedependent image potentials due to elastic surfaces distortions (Rayleigh phonons. Estimates for the lifetime of the trap ground state are given. Ions are paricularly sinsitive to electric proximity fields: for a silver substrate, we find a lifetime below one second at distrances closer than some ten 10^-6m to the surfaces. Neutral atoms may approach the surface more closely: if they have a magnetic moment, a minimum distance of one 10^-6m is estimatied in tight traps, the heat being transferred via magnetic proximity fields. For spinless atoms, heat is transferred by inelastic scattering of virtual photons off sorface phonons. The corresponding lifetime, however, is estimated to be extremely long compared to the timescale of typical experiments.
We derive the time and loss rate for a trapped atom that is coupled to fluctuating fields in the vicinity of a room-temperature metallic and/or dielectric surface. Our results indicate a clear predominance of near-field effects over ordinary blackbody radiation. We develop a theoretical framework for both charged ions and neutral atoms with and without spin. Loss processes that are due to a transition to an untrapped internal state are included.
Communicative Style
(1999)
Modelling the competitiveness of clonal plants by complementary analytical and simulation approaches
(1999)
Motivation and activity
(1999)
Clinical findings and parameter of stress and regeneration in powers before world championships
(1999)
Foreword and Introduction
(1999)
A necessary adjustment of protocol for use of DPC Coat-a-Count Total Testosterone assay with saliva
(1999)
Overshoot in giant stars
(1999)
Massive star evolution
(1999)
The gravitationally lensed quasar Q2237+0305 in X-rays: ROSAT/HRI detection of the "Einstein Cross"
(1999)
We report the first detection of the gravitationally lensed quasar Q2237+0305 in X-rays. With a ROSAT/HRI exposure of 53 ksec taken in Nov./Dec. 1997, we found a count rate of 0.006 counts per second for the combined four images. This corresponds to an X-ray flux of 2.2*E(-13) erg/cm(2) /sec and an X-ray luminosity of 4.2*E(45) erg/sec (in the ROSAT energy window 0.1-2.4 keV). The ROSAT/HRI detector is not able to resolve spatially the four quasar images (maximum separation 1.8 arcsec). The analysis is based on about 330 source photons. The signal is consistent with no variability, but with low significance. This detection is promising in view of the upcoming X-ray missions with higher spatial/spectral resolution and/or collecting power (Chandra X-ray Observatory, XMM and ASTRO-E).
Criteria of talent in sport
(1999)