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Occludin is a self-associating transmembrane tight junction protein affected in oxidative stress. However, its function is unknown. The cytosolic C-terminal tail contains a coiled coil-domain forming dimers contributing to the self- association. Studying the hypothesis that the self-association is redox-sensitive, we found that the dimerization of the domain depended on the sulfhydryl concentration of the environment in low-millimolar range. Under physiological conditions, monomers and dimers were detected. Masking the sulfhydryl residues in the domain prevented the dimerization but affected neither its helical structure nor cylindric shape. Incubation of cell extracts containing full-length occludin with sulfhydryl reagents prevented the dimerization; a cysteine/alanine exchange mutant also did not show dimer formation. This demonstrates, for the first time, that disulfide bridge formation of the domain is involved in the occludin dimerization. It is concluded that the redox-dependent dimerization of occludin may play a regulatory role in the tight junction assembly under physiological and pathological conditions.
The transmembrane tight junction protein occludin is sensitive to oxidative stress. Occludin oligomerizes; however, its function in the tight junction is unknown. The cytosolic C-terminal tail contains a coiled coil-domain and forms dimers contributing to the oligomerization. The regulation of the oligomerization remains unclear. As the domain area contains sulfhydryl residues, we tested the hypothesis that the dimerization of the coiled coil-domain depends on these residues. We showed that the dimerization is modulated by the thiol concentration in the low-millimolar range, which is relevant both for physiological and pathophysiological conditions. Masking the sulfhydryl residues in the fragment by covalent binding of 4-vinyl pyridine prevented the dimerization but did not affect its helical structure and cylindric shape. The data demonstrate, for the first time, that disulfide bridge formation of murine cystein 408 is involved in the dimerization. This process is redox-sensitive but the secondary structure of the domain is not. It is concluded that the dimerization of occludin may play a regulatory role in the tight junction assembly under physiological and pathological conditions.
In this contribution, we gather major academic and design approaches for explaining how space in games is constructed and how it constructs games, thereby defining the conceptual dimensions of gamespace. Each concept’s major inquiry is briefly discussed, iterated if applicable, as well as named. Thus, we conclude with an overview of the locative, the representational, the programmatic, the dramaturgical, the typological, the perspectivistic, the form-functional, and the form-emotive dimensions.
Two well known phenomena associated with erupting filaments are the transient coronal holes that form on each side of the filament channel and the bright post-event arcade with its expanding double row of footpoints. Here we focus on a frequently overlooked signature of filament eruptions: the spike- or fan-shaped brightenings that appear to mark the far endpoints of the filament. From a sample of non-active-region filament events observed with the Extreme- Ultraviolet Imaging Telescope on the Solar and Heliospheric Observatory, we find that these brightenings usually occur near the outer edges of the transient holes, in contrast to the post-event arcades, which define their inner edges. The endpoints are often multiple and are rooted in and around strong network flux well outside the filament channel, a result that is consistent with the axial field of the filament being much stronger than the photospheric field inside the channel. The extreme ultraviolet brightenings, which are most intense at the time of maximum outward acceleration of the filament, can be used to determine unambiguously the direction of the axial field component from longitudinal magnetograms. Their location near the outer boundary of the transient holes suggests that we are observing the footprints of the current sheet formed at the leading edge of the erupting filament, as distinct from the vertical current sheet behind the filament which is the source of the post-event arcade.
OBJECTIVE: To examine factors associated with the maternal perception of the weight status in related and unrelated children and to examine whether associated health risks for children's physical and mental health are recognized. PATIENTS AND METHODS: Two hundred nineteen mothers with children between 3 and 6 years of age took part in this study. The participating mothers were recruited from inpatient clinics and kindergartens. Parents were presented with 9 silhouettes representing different age- and gender-specific BMI percentiles. Demographic and weight-related variables were assessed with regard to their influence on the accuracy of the maternal weight estimation in general and for their own child. RESULTS: Of the participating mothers, 64.5% identified the overweight silhouettes of preschool- aged children correctly. However, only 48.8% of the mothers identified the overweight silhouettes associated with an increased risk for physical health problems, and 38.7% identified the silhouettes associated with an increased mental health risk. Mothers with a lower educational background were more likely to misclassify the overweight silhouettes and underestimate the associated health problems. For their own child, only 40.3% of the mothers chose silhouettes that were in agreement with the objective weight status of their child. This underestimation was associated with a higher maternal and child weight status but not with a general inability to identify the weight status of children. CONCLUSIONS: Identifying unrelated overweight silhouettes is influenced by maternal education level, whereas estimating their own child's weight status is influenced by the weight status of the mother and the child. Hence, feedback on the child's risk to become overweight is necessary to increase maternal risk awareness and willingness to take part in prevention programs.
Prävention kindlichen übergewichts : elterliche Selbstwirksamkeit und Handlungsergebniserwartungen
(2009)
Adipositas ist ein ernstzunehmendes gesundheitliches Problem, welches das physische und psychosoziale Wohlbefinden von Kindern und deren Eltern beeintraechtigt und somit wirksamer praeventiver Ansaetze bedarf. Dabei ist es bedeutsam, welche Barrieren, Anreize und Selbstwirksamkeitserwartungen Eltern fuer oder gegen die Teilnahme an solchen Programmen entscheiden lassen. Ziel der Studie war es zu untersuchen, wie Muetter ihre Selbstwirksamkeits- und Handlungsergebniserwartungen beschreiben und inwieweit sich soziodemografische Aspekte auf diese Einschaetzungen auswirken. Insgesamt wurden 219 Muetter von Kindern im Alter von 3-6 Jahren befragt. Mehr als die Haelfte der befragten Muetter waren uebergewichtig bzw. adipoes sowie 12% der Kinder. Es ergaben sich weder fuer das Alter und das Geschlecht des Kindes noch fuer das Familieneinkommen Unterschiede in den Handlungsergebniserwartungen. Muetter mit geringerer Bildung nahmen weniger Anreize wahr und Muetter mit uebergewichtigen Kindern sahen weniger Barrieren fuer eine Programmteilnahme. uebergewichtige Muetter dagegen bewerteten die Barrieren und Anreize hoeher als normalgewichtige Muetter. Hinsichtlich der Selbstwirksamkeit ergaben sich ebenfalls Unterschiede bezueglich der Bildung und des Gewichts der Mutter sowie des Kindes. Im Vorfeld einer Intervention sollte ein Beratungsgespraech bezogen auf die muetterlichen Erwartungen stattfinden, um die Teilnahmebereitschaft und den Programmerfolg zu unterstuetzen.
Point-of-care testing (POCT) is a fast developing area in clinical diagnostics that is considered to be one of the main driving forces for the future in vitro diagnostic market. POCT means decentralized testing at the site of patient care. The most important POCT devices are handheld blood glucose sensors. In some of these sensors, after the application of less than 1 A mu l whole blood, the results are displayed in less than 10 s. For protein determination, the most commonly used devices are based on lateral flow technology. Although these devices are convenient to use, the results are often only qualitative or semiquantitative. The review will illuminate some of the current methods employed in POCT for proteins and will discuss the outlook for techniques (e.g., electrochemical immunosensors) that could have a great impact on future POCT of proteins.
There is increasing interest ill understanding the neural systems that mediate analogical thinking, which is essential for learning and fluid intelligence. The aim of the present study was to shed light on the cerebral correlates of geometric analogical processing and on training-induced changes at the behavioral and brain level. In healthy participants a bilateral fronto-parietal network was engaged in processing geometric analogies and showed greater blood oxygenation dependent (BOLD) signals as resource demands increased. This network, as well as fusiform and subcortical brain regions, additionally showed training-induced decreases in the BOLD signal over time. The general finding that brain regions were modulated by the amount of resources demanded by the task, and/or by the reduction of allocated resources due to short term training, reflects increased efficiency - in terms of more focal and more specialized brain activation - to more economically process the geometric analogies. Our data indicate a rapid adaptation of the cognitive system which is efficiently modulated by short term training based on a positive correlation of resource demands and brain activation.