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Dieser Beitrag vergleicht die kommunale Verwaltungsdigitalisierung in Deutschland, Österreich und der Schweiz (DACH-Länder) als Vertreter der kontinentaleuropäisch-föderalen Verwaltungstradition bei zugleich unterschiedlichen Digitalisierungsansätzen und -fortschritten. Basierend auf Interviews mit 22 Expert*innen und Beobachtungen in je einer Kommune pro Land sowie Dokumenten-, Literatur- und Sekundärdatenanalysen untersucht die Studie, wie Verwaltungsdigitalisierung im Mehrebenensystem organisiert ist und welche Rolle dabei das Verwaltungsprofil spielt sowie welche Innovationsschwerpunkte die Kommunen im Hinblick auf die Leistungserbringung und die internen Prozesse setzen. Die Ergebnisse zeigen, dass der hohe Grad lokaler Autonomie den Kommunen ermöglicht, eigene Akzente in der Verwaltungsdigitalisierung zu setzen. Zugleich wirken die stark verflochtenen komplexen Entscheidungsstrukturen und hohen Koordinationsbedarfe in verwaltungsföderalen Systemen, die in Deutschland am stärksten, in Österreich etwas schwächer und in der Schweiz am geringsten ausgeprägt sind, als Digitalisierungshemmnisse. Ferner weisen die Befunde auf eine unitarisierende Wirkung der Verwaltungsdigitalisierung als Reformbereich hin. Insgesamt trägt die Studie zu einem besseren Verständnis dafür bei, welche Problematik die Verwaltungsdigitalisierung für föderal-dezentrale Verwaltungsmodelle mit sich bringt.
‘Modern talking’
(2024)
Despite growing interest, we lack a clear understanding of how the arguably ambiguous phenomenon of agile is perceived in government practice. This study aims to alleviate this puzzle by investigating how managers and employees in German public sector organisations make sense of agile as a spreading management fashion in the form of narratives. This is important because narratives function as innovation carriers that ultimately influence the manifestations of the concept in organisations. Based on a multi-case study of 31 interviews and 24 responses to a qualitative online survey conducted in 2021 and 2022, we provide insights into what public sector managers, employees and consultants understand (and, more importantly, do not understand) as agile and how they weave it into their existing reality of bureaucratic organisations. We uncover three meta-narratives of agile government, which we label ‘renew’, ‘complement’ and ‘integrate’. In particular, the meta-narratives differ in their positioning of how agile interacts with the characteristics of bureaucratic organisations. Importantly, we also show that agile as a management fad serves as a projection surface for what actors want from a modern and digital organisation. Thus, the vocabulary of agile government within the narratives is inherently linked to other diffusing phenomena such as new work or digitalisation.
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During the last decades, therapeutical proteins have risen to great significance in the pharmaceutical industry. As non-human proteins that are introduced into the human body cause a distinct immune system reaction that triggers their rapid clearance, most newly approved protein pharmaceuticals are shielded by modification with synthetic polymers to significantly improve their blood circulation time. All such clinically approved protein-polymer conjugates contain polyethylene glycol (PEG) and its conjugation is denoted as PEGylation. However, many patients develop anti-PEG antibodies which cause a rapid clearance of PEGylated molecules upon repeated administration. Therefore, the search for alternative polymers that can replace PEG in therapeutic applications has become important. In addition, although the blood circulation time is significantly prolonged, the therapeutic activity of some conjugates is decreased compared to the unmodified protein. The reason is that these conjugates are formed by the traditional conjugation method that addresses the protein's lysine side chains. As proteins have many solvent exposed lysines, this results in a somewhat uncontrolled attachment of polymer chains, leading to a mixture of regioisomers, with some of them eventually affecting the therapeutic performance.
This thesis investigates a novel method for ligating macromolecules in a site-specific manner, using enzymatic catalysis. Sortase A is used as the enzyme: It is a well-studied transpeptidase which is able to catalyze the intermolecular ligation of two peptides. This process is commonly referred to as sortase-mediated ligation (SML). SML constitutes an equilibrium reaction, which limits product yield. Two previously reported methods to overcome this major limitation were tested with polymers without using an excessive amount of one reactant.
Specific C- or N-terminal peptide sequences (recognition sequence and nucleophile) as part of the protein are required for SML. The complementary peptide was located at the polymer chain end. Grafting-to was used to avoid damaging the protein during polymerization. To be able to investigate all possible combinations (protein-recognition sequence and nucleophile-protein as well as polymer-recognition sequence and nucleophile-polymer) all necessary building blocks were synthesized. Polymerization via reversible deactivation radical polymerization (RDRP) was used to achieve a narrow molecular weight distribution of the polymers, which is required for therapeutic use.
The synthesis of the polymeric building blocks was started by synthesizing the peptide via automated solid-phase peptide synthesis (SPPS) to avoid post-polymerization attachment and to enable easy adaptation of changes in the peptide sequence. To account for the different functionalities (free N- or C-terminus) required for SML, different linker molecules between resin and peptide were used.
To facilitate purification, the chain transfer agent (CTA) for reversible addition-fragmentation chain-transfer (RAFT) polymerization was coupled to the resin-immobilized recognition sequence peptide. The acrylamide and acrylate-based monomers used in this thesis were chosen for their potential to replace PEG.
Following that, surface-initiated (SI) ATRP and RAFT polymerization were attempted, but failed. As a result, the newly developed method of xanthate-supported photo-iniferter (XPI) RAFT polymerization in solution was used successfully to obtain a library of various peptide-polymer conjugates with different chain lengths and narrow molar mass distributions.
After peptide side chain deprotection, these constructs were used first to ligate two polymers via SML, which was successful but revealed a limit in polymer chain length (max. 100 repeat units). When utilizing equimolar amounts of reactants, the use of Ni2+ ions in combination with a histidine after the recognition sequence to remove the cleaved peptide from the equilibrium maximized product formation with conversions of up to 70 %.
Finally, a model protein and a nanobody with promising properties for therapeutical use were biotechnologically modified to contain the peptide sequences required for SML. Using the model protein for C- or N-terminal SML with various polymers did not result in protein-polymer conjugates. The reason is most likely the lack of accessibility of the protein termini to the enzyme. Using the nanobody for C-terminal SML, on the other hand, was successful. However, a similar polymer chain length limit was observed as in polymer-polymer SML. Furthermore, in case of the synthesis of protein-polymer conjugates, it was more effective to shift the SML equilibrium by using an excess of polymer than by employing the Ni2+ ion strategy.
Overall, the experimental data from this work provides a good foundation for future research in this promising field; however, more research is required to fully understand the potential and limitations of using SML for protein-polymer synthesis. In future, the method explored in this dissertation could prove to be a very versatile pathway to obtain therapeutic protein-polymer conjugates that exhibit high activities and long blood circulation times.
Verträge über nicht-personenbezogene Daten werden seit dem Data Act immer wichtiger. Gleichwohl ist deren rechtliche Qualifikation immer noch nicht abschließend geklärt. Auch wenn klar ist, dass Daten in aller Regel keinem geistigen Eigentumsrecht unterliegen, nur in seltenen Fällen Know-how sind und an ihnen kein Sacheigentum bestehen kann, benötigen die Verträge eine hinreichende rechtliche Grundlage ihres Gegenstands. Der Beitrag plädiert für eine geringfügige Anpassung, jedenfalls in der Technologiegruppenfreistellungsverordnung.
This article analyses incremental institutional change and subsequent organizational and performance outcomes of the digital transformation from a comparative perspective. Through 31 expert interviews, the authors compare two digitalized public services in Germany. Two digitalization approaches are identified. The voluntary, decentralized bottom-up approach involves layering of new rules, limited organizational restructuring, and performance deficits. Conversely, the compulsory, top-down approach with centralized control facilitates displacement of existing rules and far-reaching organizational change; in this study, it is also associated with improved performance.
Zuerst erschienen in:
Alexander von Humboldt-Stiftung. Mitteilungen, 5. Jg., Heft 38, Oktober 1980, S. 27–36.
Off-road adventures
(2024)
This article focuses on the visual qualities of Alexander von Humboldt’s statistical tables in his Political Essay on the Kingdom of New Spain (1808–1811, 2nd ed. 1825–1827) with special attention to how such composites of numbers, alphabetical script, and semiotic elements relate to narrative writing. I argue that Humboldt’s tables/tableaus open up spaces inside his narrative that fragment the reading process, inviting new conversations, connections, and ideas.
Die deutsch-kubanische Forschungs- und Digitalisierungsinitiative „Proyecto Humboldt Digital“ (ProHD) hat während ihrer Projektlaufzeit (2019–2023) wichtige Quellen zum Thema „Humboldt und Kuba“ erstmals digital erschlossen. Als Kooperation zwischen der Berlin-Brandenburgischen Akademie der Wissenschaften und der Oficina del Historiador de la Ciudad de La Habana hat ProHD damit wichtige Akzente für die Archivdigitalisierung, die digitale Editionsphilologie und die digitale Wissenschaftskommunikation in Kuba gesetzt. Das Korpus der erschlossenen Bestände wird hier in fünf Schlaglichtern vorgestellt: 1) Quellen zur Humboldt’schen Forschungsreise, 2) Juan Luis de la Cuesta, 3) Materialien zu Kuba aus dem Humboldt-Nachlass, 4) Zensur und Beschlagnahme des Essai politique sur l’île de Cuba, 5) Francisco de Arango y Parreño.