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Selected enzymes (alpha-amylase, trypsin, and lysozyme) were allowed to react with some simple phenolic and related compounds (caffeic acid, chlorogenic acid, ferulic acid, gallic acid, m-, o-, and p-dihydroxybenzenes, quinic acid, and p-benzoquinone). The derivatized enzymes obtained were characterized in terms of their activity. In vitro experiments showed that the enzymatic activity of the derivatives was adversely affected. This enzyme inhibition depended on the reactivity of the phenolic and related substances tested as well as on the kind of substrate applied. The decrease in the activity was accompanied by a reduction in the amount of free amino and thiol groups, as well as tryptophan residues, which resulted from the covalent attachment of the phenolic and related compounds to these reactive nucleophilic sites in the enzymes. The enzyme inhibition correlates well with the blocking of the mentioned amino acid side chains.
Secondary plant metabolites are important native food components, which are becoming more and more interesting due to their physiological effects on humans. Some representatives of these compounds are very reactive and can interact with other main food components like proteins resp. enzymes. This work deals with the reactions of selected enzymes (trypsin, alpha-chymotrypsin and alpha-amylase) with simple phenolic and related substances (caffeic acid, chlorogenic acid, ferulic acid, gallic acid, meta-, ortho- and para-dihydroxybenzene, 1,4-benzoquinone, quinic acid). The derivatives formed were characterized in terms of their activity and selected physicochemical properties. In vitro experiments showed that the proteolytic digestion of food proteins with trypsin and alpha-chymotrypsin derivatives was adversely affected. This decrease depends on the reactivity of the phenolic and related substances tested as well as on the kind of substrate applied. Reactions of phenolic compounds with other enzymes (alpha-amylase and lysozyme) showed similar results with regard to physicochemical properties and their activities.
In the CPMAS spectra of soil samples, the paramagnetic soil constituents partially discriminate against the Signal intensity of the 13C atoms.The aim of the study was to check to what extend the signal depression is selective to a certain kind of carbon species. A strong linear correlation between the detectability of carbon and the cube root of wt.% Fe was observed for both, the total signal and the signal of the carbon species as well. For a C:Fe ratio of 1,5 - 20 non selective signal loss caused by the iron particles dominates. Despite the high percentage of non-detectable carbon the 13C CPMAS spectra correctly reflect the relative composition of soil organic matter
Selected food proteins (myoglobin and soy glycinin) were caused to react with flavonoids (apigenin and quercetin) to estimate the influence of the number and the position of hydroxy substituents. The protein derivatives formed have been charcterized in terms of their properties where they showed changes in the content of free amino groups, tryptophan, and thiol groups. The myoglobin derivatives have also been characterized in terms of their solubility at different pH-values to document the influence on the functional properties. The influence of myoglobin derivatives on the in vitro digestibility with trypsin was also demonstrated, with the digestion of the derivatized myoglobin being favored.
Towards a better understanding of laser beam melt ablation using methods of statistical analysis
(2002)
Laser beam melt ablation, as a contact free machining process, offers several advantages compared to conventional processing mechanisms. Although the idea behind it is rather simple, the process has a major limitation: with increasing ablation rate surface quality of the workpiece processed declines rapidly. The structures observed show a clear dependence of the line energy. In dependence of this parameter several regimes of the process have been separated. These are clearly distinguishable as well in the surfaces obtained as in the signals gained by the measurement of the process emissions which is the observed quantity chosen.
Recurrence-plot-based measures of complexity and its application to heart-rate-variability data
(2002)
The knowledge of transitions between regular, laminar or chaotic behavior is essential to understand the underlying mechanisms behind complex systems. While several linear approaches are often insufficient to describe such processes, there are several nonlinear methods which however require rather long time observations. To overcome these difficulties, we propose measures of complexity based on vertical structures in recurrence plots and apply them to the logistic map as well as to heart rate variability data. For the logistic map these measures enable us not only to detect transitions between chaotic and periodic states, but also to identify laminar states, i.e. chaos-chaos transitions. The traditional recurrence quantification analysis fails to detect the latter transitions. Applying our new measures to the heart rate variability data, we are able to detect and quantify the laminar phases before a life-threatening cardiac arrhythmia occurs thereby facilitating a prediction of such an event. Our findings could be of importance for the therapy of malignant cardiac arrhythmias.
Art. Kategorie prázdná
(2002)
In mathematical models, very simple communities consisting of three or more species frequently display chaotic dynamics which implies that long-term predictions of the population trajectories in time are impossible. Communities in the wild tend to be more complex, but evidence for chaotic dynamics from such communities is scarce. We used supercomputing power to test the hypothesis that chaotic dynamics become less frequent in model ecosystems when their complexity increases. We determined the dynamical stability of a universe of mathematical, nonlinear food web models with varying degrees of organizational complexity. We found that the frequency of unpredictable, chaotic dynamics increases with the number of trophic levels in a food web but decreases with the degree of complexity. Our results suggest that natural food webs possess architectural properties that may intrinsically lower the likelihood of chaotic community dynamics.
Katalytische Umwandlung von "Flur-Chlor-Kohlenwasserstoffen" zu umweltverträglichen Verbindungen
(2002)
We employ classical statistical methods of multivariate classification for the exploitation of the stellar content of the Hamburg/ESO objective prism survey (HES). In a simulation study we investigate the precision of a three- dimensional classification (Teff, log g, [Fe/H]) achievable in the HES for stars in the effective temperature range 5200 K<Teff<6800 K, using Bayes classification. The accuracy in temperature determination is better than 400 K for HES spectra with S/N>10 (typically corresponding to BJ<16.5). The accuracies in log g and [Fe/H] are better than 0.68 dex in the same S/N range. These precisions allow for a very efficient selection of metal-poor stars in the HES. We present a minimum cost rule for compilation of complete samples of objects of a given class, and a rejection rule for identification of corrupted or peculiar spectra. The algorithms we present are being used for the identification of other interesting objects in the HES data base as well, and they are applicable to other existing and future large data sets, such as those to be compiled by the DIVA and GAIA missions.
We study the optical forces due to the radiation of a thermal source. Our model consists of a particle modelled by a dipole above a half-space at temperature T. The fluctuating fields are computed using the Lifshitz model. We find two contributions to the force: a repulsive "wind" component and a dispersive force mainly due to the contribution of thermally excited surface waves. It is found that for SIC material, the latter is repulsive in the very near field. The usual van der Waals force is larger by a factor of approximately ten for submicron size particles.
Formation of a buried density grating on thermal erasure of azobenzene polymer surface gratings
(2002)
Photoaddressable alignment layers for fluorescent polymers in polarized electroluminescence devices
(2002)
Chiroptcial properties of poly(p-phenyleneethynylene) copolymers in thin films : large g-values
(2002)
We calculate the phonon excistation spectrum in a zero-temperature dilute boson-fermion gaseous mixture. We show how the sound velocity changes due to the boson-fermion interaction, and we determine the dynamical stability regime of a homogeneous mixture. We identify a resonant phonon-exchange interaction between the fermions as the physical mechanism leading to the instability.