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- contrast (4)
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- core (4)
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- correlations (4)
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- creep (4)
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- cue-based retrieval (4)
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- events (4)
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- fabrication (4)
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- family (4)
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- finger counting (4)
- first-passage time (4)
- fish (4)
- flood (4)
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- fluorescence microscopy (4)
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- galaxies: fundamental parameters (4)
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- locus of control (4)
- low-energy electrons (4)
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- methods: statistical (4)
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- organic matter (4)
- orthography (4)
- oxygen (4)
- p53 (4)
- palladium (4)
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- mantle transition zone (2)
- margins of error (2)
- marine (2)
- marine governance (2)
- marine terraces (2)
- marriage (2)
- mass media (2)
- master narratives (2)
- mastication (2)
- matching (2)
- materials science (2)
- maternal effects (2)
- mathematics instruction (2)
- mating (2)
- maximal subsemigroups (2)
- maximum a posteriori (2)
- maximum magnitude (2)
- mean squared displacement (2)
- measles virus (2)
- measurement error (2)
- mechanical (2)
- mechanical properties (2)
- mechanical tendinous oscillations (2)
- mechanistic models (2)
- medicine (2)
- melts (2)
- membrane proteins (2)
- memory and delay (2)
- mental imagery (2)
- meropenem (2)
- mesocosm (2)
- metaanalysis (2)
- metabolic disease (2)
- metadata (2)
- metagenome (2)
- metal coordination (2)
- metal-organic frameworks (2)
- metamorphosis of international law (2)
- metaphor (2)
- meteorological extremes (2)
- method comparison (2)
- method development (2)
- methodological quality (2)
- methods: MHD (2)
- methyl orange (2)
- methylene blue (2)
- methylmercury (2)
- mica (2)
- micelles (2)
- micro-CT (2)
- microRNA (2)
- microbes (2)
- microbial (2)
- microbial communities (2)
- microbial community (2)
- microbial interactions (2)
- microcapsules (2)
- microclimate (2)
- microcracking (2)
- microemulsion (2)
- microgel (2)
- micronutrients (2)
- microparticle (2)
- microtubule-organization (2)
- microviridins (2)
- mid-temperature transition (2)
- migrants (2)
- mild cognitive impairment (2)
- military culture (2)
- minimal pairs (2)
- minimax convergence rates (2)
- minorities (2)
- mirror (2)
- mirror illusion (2)
- mirror therapy (2)
- miscellaneous (2)
- miscibility gap (2)
- missing-VP effect (2)
- mitochondrial dysfunction (2)
- mitogenome (2)
- mitogenomes (2)
- mixtures (2)
- mobile applications (2)
- mobile health (2)
- mobile links (2)
- mobile phone (2)
- modal verbs (2)
- model coupling (2)
- model validation (2)
- model verification (2)
- modeling language (2)
- modernity (2)
- modified Mannich reaction (2)
- modular production (2)
- molecular architecture (2)
- molecular clock (2)
- molecular dynamics simulations (2)
- molecular weight (2)
- molecules (2)
- molybdenum (2)
- moment tensor (2)
- monoclonal antibodies (2)
- monolayer formation (2)
- monomer (2)
- monomers (2)
- moral disengagement (2)
- morality (2)
- morpho-orthography (2)
- morphogenesis (2)
- morphological analysis (2)
- morphological decomposition (2)
- morphological priming (2)
- morphologically complex words (2)
- morphosyntax (2)
- motion capture (2)
- motivation in mathematics (2)
- motivational interviewing (2)
- motor resonance (2)
- motor skills (2)
- motor system (2)
- movement kinematics (2)
- movement patterns (2)
- mowing (2)
- multi-agent system (2)
- multi-level system (2)
- multi-nutrient limitation (2)
- multicultural policy (2)
- multiculturalism (2)
- multidiversity (2)
- multidrug resistance (2)
- multifunctional polymers (2)
- multilateralism (2)
- multilevel analyses (2)
- multilevel analysis (2)
- multiple (2)
- multiple sclerosis (2)
- multiscale analysis (2)
- multispectral (2)
- multivariate regression (2)
- multivariate statistics (2)
- murchison meteorite (2)
- muscle action (2)
- muscle metabolism (2)
- muscle oxygen saturation (2)
- muscle physiology (2)
- muscle spindle (2)
- muscle stability (2)
- muscle weakness (2)
- musculoskeletal (2)
- musculoskeletal ultrasound (2)
- music (2)
- music cognition (2)
- music industry (2)
- music information retrieval (2)
- music perception (2)
- mutants (2)
- mutation (2)
- myocardial infarction (2)
- myopathy (2)
- n-Alkanes (2)
- naming (2)
- nanoclusters (2)
- nanocomposites (2)
- nanoelectrodes (2)
- nanogranites (2)
- nanolenses (2)
- nanoparticle dimers (2)
- nanoreactor (2)
- naphthalenes (2)
- natal dispersal (2)
- national identity (2)
- natural climate solutions (2)
- natural disasters (2)
- natural habitats (2)
- natural hazard (2)
- naturalism (2)
- naturalized species (2)
- nature conservation (2)
- nearshore zone (2)
- need satisfaction (2)
- needs (2)
- negation (2)
- negative numbers (2)
- negative strengthening (2)
- neo-institutionalism (2)
- neo-liberalism (2)
- neogene (2)
- neotectonics (2)
- network dynamics (2)
- neurodegenerative diseases (2)
- neuroenhancement (2)
- neuromuscular functioning (2)
- neuronal networks (2)
- neuropsychology (2)
- neutrality (2)
- neutron powder diffraction (2)
- neutron reflectometry (2)
- neutron stars (2)
- new combination (2)
- next generation sequencing (2)
- niche and fitness differences (2)
- niche theory (2)
- niche width (2)
- nitride materials (2)
- nitrile (2)
- nitrogen fixation (2)
- nitrogen limitation (2)
- nitrogen-doped carbon (2)
- noisy systems (2)
- nominalization (2)
- non-Gaussian (2)
- non-Langevin recombination (2)
- non-athletes (2)
- non-categoricity (2)
- non-equilibrium (2)
- non-native speech perception (2)
- non-predatory mortality (2)
- nonfullerene acceptors (2)
- nonlinear filtering (2)
- nonlinear operator (2)
- nonlocal coupling (2)
- nonmodel species (2)
- nonradiative recombination (2)
- nonradiative voltage losses (2)
- nonstationarity (2)
- normal faults (2)
- noun (2)
- novae, cataclysmic variables (2)
- novel ecosystems (2)
- novelty (2)
- nucleation (2)
- nucleosome (2)
- number and brightness (2)
- number concepts (2)
- number processing (2)
- numerical distance effect (2)
- numerical model (2)
- nursing home (2)
- nutrient (2)
- nutrient cycling (2)
- nutrient retention (2)
- nutrient stoichiometry (2)
- nutrient supply (2)
- oarsmen (2)
- object search (2)
- observation (2)
- occlusion (2)
- ocean acidification (2)
- ocean color remote sensing (2)
- ocean heat uptake (2)
- oil (2)
- oil yield (2)
- older persons (2)
- oligodepsipeptides (2)
- oligomerization (2)
- omics (2)
- on-water performance (2)
- oncology (2)
- one-carbon metabolism (2)
- online (2)
- online course (2)
- online disinhibition (2)
- ontogenesis (2)
- ontogeny (2)
- opacity (2)
- open (2)
- open clusters and associations: general (2)
- open data (2)
- open education (2)
- open innovation (2)
- open-field (2)
- operator-valued symbols (2)
- oppositions (2)
- optical imaging (2)
- optical manipulation (2)
- optical remote sensing (2)
- optical sensors (2)
- optionality (2)
- orbifolds (2)
- orchard management (2)
- orchestration (2)
- organ growth (2)
- organic carbon (2)
- organic chemistry (2)
- organic electronics (2)
- organization theory (2)
- organizational change (2)
- organizational reform (2)
- origins (2)
- oroclinal bending (2)
- orogenic peridotite (2)
- orogenic processes (2)
- orogeny (2)
- orographic rainfall (2)
- orthogroup (2)
- orthopedic (2)
- osmotic-pressure (2)
- osteoblast (2)
- other-race effect (2)
- outbreak (2)
- outcome measures (2)
- overland flow (2)
- overreaching (2)
- overtraining (2)
- overuse injuries (2)
- oxidation state (2)
- oxygen heterocycles (2)
- oxygen plasma (2)
- oxygenation (2)
- p-Aminophenol (2)
- pH-responsive (2)
- pQCT (2)
- palaeoenvironmental proxies (2)
- palaeoenvironmental reconstruction (2)
- paleo-sediment (2)
- paleoceanography (2)
- paleoecology (2)
- paleovegetation (2)
- palmitoylation (2)
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- parameter (2)
- parameterization (2)
- parameters (2)
- parasitoid (2)
- parchment (2)
- parental leave (2)
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- parenting (2)
- parents (2)
- partial least squares (2)
- particle filter (2)
- particles (2)
- particulate matter (2)
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- party competition (2)
- pasture (2)
- pathway (2)
- pathways (2)
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- pea (2)
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- peak height velocity (2)
- peak torque (2)
- pediatrics (2)
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- peptide (2)
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- perception of robots (2)
- perceptual reorganization (2)
- performance budget (2)
- performance information (2)
- performance measurement (2)
- periphyton (2)
- permittivity enhancement (2)
- permutation entropy (2)
- perovskite solar cell (2)
- personality change (2)
- personality traits (2)
- perspective taking (2)
- persulfide (2)
- pesticides (2)
- phagocytosis (2)
- pharmacokinetics (2)
- phase behavior (2)
- phase dynamics (2)
- phase morphology (2)
- phase transfer (2)
- phenolic compounds (2)
- phenology (2)
- phenomics (2)
- phonological awareness (2)
- phosphatidylserine (2)
- phosphoglucan (2)
- phosphoproteomics (2)
- phosphorylase (2)
- photo-isomerization (2)
- photocatalysts (2)
- photochromism (2)
- photodehydro-Diels-Alder reaction (2)
- photoelectron (2)
- photogrammetry (2)
- photoisomerization (2)
- photoluminescence quenching (2)
- photon density wave spectroscopy (2)
- photonic crystals (2)
- photosensitive surfactants (2)
- photoswitches (2)
- photosynthetic rate (2)
- phreeqc (2)
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- pigment composition (2)
- pitch angle (2)
- planetary boundaries (2)
- planetary rings (2)
- planetary systems (2)
- planets and satellites: composition (2)
- planets and satellites: fundamental parameters (2)
- planets and satellites: gaseous planets (2)
- planets and satellites: general (2)
- plant architecture (2)
- plant invasions (2)
- plant species richness (2)
- plant traits (2)
- plant volatiles (2)
- plant-soil (belowground) interactions (2)
- plants (2)
- plasma density (2)
- plasma instabilities (2)
- plasmasphere (2)
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- plastid transformation (2)
- plateau (2)
- platform innovation (2)
- platinum (2)
- plausibility (2)
- playa (2)
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- plyometric exercise (2)
- plyometrics (2)
- polarity (2)
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- policy reform (2)
- policy-making (2)
- political ideology (2)
- pollinator shift (2)
- poly(epsilon-caprolactone) (2)
- polyadenylation (2)
- polyampholytes (2)
- polycystic ovary syndrome (2)
- polyelectrolytes (2)
- polyester (2)
- polyesterurethanes (2)
- polymersomes (2)
- polysemy (2)
- polystyrene-block-poly(4-vinylpyridine) (2)
- population cycles (2)
- population genetics (2)
- population growth rate (2)
- population pharmacokinetics (2)
- population size (2)
- population structure (2)
- pore pressure (2)
- pornography (2)
- porous silicon (2)
- positioning (2)
- positive solutions (2)
- possessives (2)
- post-natal (2)
- posttraumatic stress disorder (2)
- postural sway (2)
- potassium channel (2)
- power spectral analysis (2)
- pp-wave solutions (2)
- practice (2)
- pragmaticalisation (2)
- pragmatics (2)
- pre-activity (2)
- pre-natal (2)
- pre-school (2)
- pre-service teachers (2)
- precaution (2)
- predation (2)
- predation risk (2)
- predator (2)
- predictability (2)
- prediction error (2)
- predictive coding (2)
- predictive models (2)
- predictive systems ecology (2)
- preference (2)
- preference assessment (2)
- preference for agency (2)
- preferential flow (2)
- prefixes (2)
- prefrontal cortex (2)
- preprocessing (2)
- presidentialism (2)
- pressure (2)
- pressures (2)
- prestige (2)
- presupposition (2)
- prevalence information (2)
- primary care (2)
- printing (2)
- prior knowledge (2)
- privatization (2)
- proactive personality (2)
- proactive work behavior (2)
- probability (2)
- probability density function (2)
- problem (2)
- problem-solving (2)
- problems (2)
- process (2)
- process analytical technology (2)
- process mining (2)
- process modeling (2)
- process modelling (2)
- process simulation (2)
- process tracing (2)
- process-based model (2)
- process-based modeling (2)
- processes (2)
- product generation engineering (2)
- production planning (2)
- prognosis (2)
- proliferation (2)
- prolonged grief disorder (2)
- proper motions (2)
- property rights (2)
- prosocial (2)
- prosody processing (2)
- prospective (2)
- protease inhibitors (2)
- protected area (2)
- protection motivation theory (2)
- protein carbonyls (2)
- protein engineering (2)
- protein modification (2)
- protein restriction (2)
- protein search (2)
- protein translocation (2)
- protein-protein (2)
- proteomic analysis (2)
- protest (2)
- protonation (2)
- prototype-willingness-model (2)
- psycho-oncology (2)
- psychological detachment (2)
- psychological distress (2)
- psychological well-being (2)
- psychologinguistics (2)
- psychopathy (2)
- psychophysics (2)
- psychopower (2)
- psychosocial moderators (2)
- puberty (2)
- public (2)
- public engagement (2)
- public good (2)
- public management (2)
- public policy (2)
- public sector reform (2)
- publication bias (2)
- pulling isometric muscle action (PIMA) (2)
- pulsars: individual: PSR B0833-45 (2)
- purification (2)
- quality assurance (2)
- quantum (2)
- quantum field theory (2)
- quartz crystal microbalance (2)
- quasi-Fermi level splitting (2)
- quenched energy landscape (2)
- questioning solutions (2)
- radhard design (2)
- radial flow (2)
- radiation mechanisms: nonthermal (2)
- radiation therapy (2)
- radiative forcing (2)
- radiocarbon dating (2)
- radiogenic isotopes (2)
- radiography (2)
- radiosensitizers (2)
- rainfall (2)
- rainfall gradient (2)
- rampage (2)
- random diffusivity (2)
- random matrix theory (2)
- random number generation (2)
- random-walks (2)
- randomized controlled trial (2)
- range dynamics (2)
- range of motion (2)
- range size (2)
- rank (2)
- ranking (2)
- rapid test kit (2)
- rare events (2)
- rate of perceived exertion (2)
- rate of torque development (2)
- ray tracing (2)
- reactance (2)
- reaction kinetics theory (2)
- reaction monitoring (2)
- reactive transport modeling (2)
- reading motivation (2)
- real-time (2)
- receiver function (2)
- reciprocal class (2)
- recollection (2)
- recollimation shocks (2)
- recombinant inbred line (2)
- recombination order (2)
- record (2)
- record linkage (2)
- recreational sport (2)
- recurrence analysis (2)
- recurrence quantification analysis (2)
- redemption (2)
- redox homeostasis (2)
- redox polymers (2)
- redox processes (2)
- redox state (2)
- reduction (2)
- reed (2)
- referential coding (2)
- reflexives (2)
- reforms (2)
- refugee crisis (2)
- refugee youth (2)
- regional development (2)
- regional flood frequency analysis (2)
- regionalisation (2)
- regularity (2)
- regulations (2)
- regulatory T cells (2)
- regulatory physiology (2)
- reinforcement (2)
- relations (2)
- relationship (2)
- relationship constellations (2)
- relative pollen productivity (2)
- relaxation (2)
- relaxor-ferroelectric polymer (2)
- remission (2)
- remote teaching (2)
- renormalization Hopf algebra (2)
- replacement (2)
- repository far-field (2)
- reproducing kernel Hilbert space (2)
- reproductive strategies (2)
- reproductive success (2)
- resampling (2)
- research challenges (2)
- research design (2)
- research framework (2)
- research priorities (2)
- research transparency (2)
- reservoir (2)
- reservoir characterization (2)
- resetting (2)
- resolution infrared-spectroscopy (2)
- resonance energy transfer (2)
- resonances (2)
- resource allocation (2)
- resource selection (2)
- restricted range (2)
- resurrection plants (2)
- retentivity (2)
- retrogressive thaw slumps (2)
- retrospective (2)
- return to work (2)
- revegetation (2)
- reversible measure (2)
- reward anticipation (2)
- rewards (2)
- rhizosphere (2)
- rhythm (2)
- ribosome assembly (2)
- rifting (2)
- ring current (2)
- ring current electrons (2)
- risk assessment (2)
- risk aversion (2)
- risk communication (2)
- risk governance (2)
- risk management cycle (2)
- risk perceptions (2)
- risk-factors (2)
- risks (2)
- river erosion (2)
- river floods (2)
- rock (2)
- rock glacier (2)
- role-play (2)
- rolling averages (2)
- root morphology (2)
- root water uptake (2)
- rotational diffusion (2)
- rotifer (2)
- runners (2)
- running mechanics (2)
- rupture (2)
- rupturing of metal film (2)
- réchauffement planétaire (2)
- sACC (2)
- sadness (2)
- salamanders (2)
- salience (2)
- saliency (2)
- salinity (2)
- salivary alpha-amylase (2)
- salivary gland (2)
- salt (2)
- salt pan (2)
- salt structures (2)
- sample preparation (2)
- satellite data (2)
- satisfaction (2)
- savanna ecology (2)
- scaffolding (2)
- scalability (2)
- scale-dependence (2)
- scanning tunneling microscopy (2)
- scene perception (2)
- school learning (2)
- school mathematics (2)
- school quality (2)
- school shooting (2)
- science (2)
- science mapping (2)
- scoping review (2)
- scrambling (2)
- sea level (2)
- sea level rise (2)
- second chambers (2)
- second language processing (2)
- secular changes (2)
- secular variation (2)
- sedentary (2)
- sediment core (2)
- sediment routing (2)
- sediment storage (2)
- sediment supply (2)
- sediment transport (2)
- sediment yield (2)
- sediments (2)
- seed (2)
- seed dispersal syndrome (2)
- seed provisioning (2)
- segmentation (2)
- seismic monitoring (2)
- seismology (2)
- selective autophagy (2)
- selective laser melting (2)
- selective laser melting (SLM) (2)
- selenoprotein P (2)
- self threat (2)
- self-assembled monolayers (2)
- self-control (2)
- self-determination theory (2)
- self-disclosure (2)
- self-enhancement (2)
- self-evaluation (2)
- self-harm (2)
- self-incompatibility (2)
- self-isolation (2)
- self-objectification (2)
- self-organisation (2)
- self-regulated learning (2)
- self-similarity (2)
- self-sovereign identity (2)
- semantic transparency (2)
- semi-arid hydrology (2)
- semi-parliamentarism (2)
- semiconducting polymers (2)
- semiconductor lasers (2)
- sensor (2)
- sensor alignment (2)
- sensor data (2)
- sensor fusion (2)
- sensors and actuators (2)
- sentence parsing (2)
- sentinel-1 (2)
- separation of powers (2)
- sequential data assimilation (2)
- serious game (2)
- serum (2)
- sexual aggression perpetration (2)
- sexual behavior (2)
- sexual coercion (2)
- sexual dimorphism (2)
- sexual reproduction (2)
- sexual self-esteem (2)
- sexual victimisation (2)
- sexual violence (2)
- shadow education (2)
- shallow structure hypothesis (2)
- shieldings (TSNMRS) (2)
- shoe (2)
- short latency response (2)
- short-read mapping (2)
- shortening (2)
- shotgun sequencing (2)
- shoulder (2)
- shrub-encroachment (2)
- sibilant (2)
- sick leave (2)
- signal propagation (2)
- signalling (2)
- silica (2)
- silver (2)
- simulation model (2)
- simulation-based (2)
- simultaneous bilingualism (2)
- single event transients (2)
- single-case experimental design (2)
- single-cell analysis (2)
- single-particle tracking (2)
- situational strength (2)
- size (2)
- size distribution (2)
- skeletal muscle (2)
- skill (2)
- slope aspect (2)
- smart materials (2)
- soccer players (2)
- social class (2)
- social environment (2)
- social epistemology (2)
- social inequality (2)
- social interaction (2)
- social modulation (2)
- social network (2)
- social reactivity (2)
- social robots (2)
- socioeconomy (2)
- sodium hydroxide etching (2)
- soft errors (2)
- soft robotics (2)
- soft-templating (2)
- software process improvement (2)
- soil fatigue (2)
- soil landscape (2)
- soils (2)
- solar powered light-emitting diode (2)
- solubility (2)
- solvent vapor annealing (2)
- somatic variables (2)
- source parameters (2)
- source-sink dynamics (2)
- soy protein (2)
- space use (2)
- space-charge stability (2)
- spacing (2)
- spatial autocorrelation (2)
- spatial heterogeneity (2)
- spatial scales (2)
- spatial vision (2)
- spatially explicit model (2)
- speakers (2)
- special education (2)
- special educational needs (2)
- species assembly (2)
- species diversity (2)
- species interactions (2)
- species recognition (2)
- specific language impairment (2)
- specificity (2)
- specifier proteins (2)
- spectral analysis (2)
- spectroscopic ellipsometry (2)
- speech motor control (2)
- speech segmentation (2)
- speed-accuracy trade-off (2)
- speleothems (2)
- sphingomyelin (2)
- sphingosine kinase (2)
- spider silk (2)
- spin transition (2)
- spin-orbit coupling (2)
- spin-orbit interaction (2)
- split Hopkinson pressure bar (2)
- splitting (2)
- sport (2)
- sports medicine (2)
- sprint (2)
- sprints (2)
- stable carbon isotopes (2)
- stable matching (2)
- stakeholder engagement (2)
- standard (2)
- starch granule initiation (2)
- starch granule morphology (2)
- starch granules (2)
- starch morphology (2)
- starch synthase (2)
- stars: flare (2)
- stars: individual ([HD 36486]delta Ori A) (2)
- stars: individual (gamma Cassiopeiae) (2)
- stars: individual: WR 102c (2)
- stars: individual: WR 102ka (2)
- stars: jets (2)
- stars: solar-type (2)
- stars: variables: Cepheids (2)
- starvation (2)
- statistical methods (2)
- statistical tools (2)
- stem cell adhesion (2)
- step selection (2)
- stereoselectivity (2)
- stereotypes (2)
- sterol (2)
- stimulus-response compatibility (2)
- stokes shift (2)
- storage capacity (2)
- strain localization (2)
- strategic growth adjustment (2)
- strategy (2)
- stratification (2)
- streptavidin (2)
- stressors (2)
- strong semilattice of semigroups (2)
- structural change (2)
- structural health monitoring (2)
- structural thermodynamics (2)
- structures (2)
- study abroad (2)
- study designs (2)
- stumbling (2)
- style transfer (2)
- subcellular localization (2)
- subcutaneous adipose tissue (2)
- subduction initiation (2)
- subject-oriented learning (2)
- submarine (2)
- submillimetre: ISM (2)
- subsea permafrost (2)
- substituent effects (2)
- substitution effects (2)
- subsurface life (2)
- succession (2)
- sufficiency (2)
- suicidality (2)
- sulfite oxidase (2)
- superalloys (2)
- superoxide dismutase (2)
- supersaturated species coexistence (2)
- supervised machine learning (2)
- supervisor support (2)
- supply chains (2)
- support vector machines (2)
- supramolecular chemistry (2)
- surface charge (2)
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Electrochemical methods offer great promise in meeting the demand for user-friendly on-site devices for monitoring important parameters. The food industry often runs own lab procedures, for example, for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with smart technologies. Enzyme-linked immunosorbent assays, with photometric detection of 3,3',5,5'-tetramethylbenzidine (TMB), form a good basis for sensitive detection. To provide a straightforward approach for the miniaturization of the detection step, we have studied the pitfalls of the electrochemical TMB detection. By cyclic voltammetry it was found that the TMB electrochemistry is strongly dependent on the pH and the electrode material. A stable electrode response to TMB could be achieved at pH 1 on gold electrodes. We created a smartphone-based, electrochemical, immunomagnetic assay for the detection of ochratoxin A in real samples, providing a solid basis for sensing of further analytes.
Solar filaments often erupt partially. Although how they split remains elusive, the splitting process has the potential of revealing the filament structure and eruption mechanism. Here we investigate the pre-eruption splitting of an apparently single filament and its subsequent partial eruption on 2012 September 27. The evolution is characterized by three stages with distinct dynamics. During the quasi-static stage, the splitting proceeds gradually for about 1.5 hr, with the upper branch rising at a few kilometers per second and displaying swirling motions about its axis. During the precursor stage that lasts for about 10 minutes, the upper branch rises at tens of kilometers per second, with a pair of conjugated dimming regions starting to develop at its footpoints; with the swirling motions turning chaotic, the axis of the upper branch whips southward, which drives an arc-shaped extreme-ultraviolet front propagating in a similar direction. During the eruption stage, the upper branch erupts with the onset of a C3.7-class two-ribbon flare, while the lower branch remains stable. Judging from the well-separated footpoints of the upper branch from those of the lower one, we suggest that the pre-eruption filament processes a double-decker structure composed of two distinct flux bundles, whose formation is associated with gradual magnetic flux cancellations and converging photospheric flows around the polarity inversion line.
Although distally steepened carbonate ramps have been studied by numerous researchers, the processes that control the development of these carbonate systems, including tectonics, differential carbonate production along the ramp profile, or antecedent physiography of the slopes, are an ongoing discussion. We use a stratigraphic forward model to test different hypotheses to unravel controls over distally steepened ramp development, referenced to the well-known Upper Miocene Menorca carbonate ramp (Spain). Sensitivity tests show that distally steepened ramps develop under complex interaction among accommodation, carbonate production and sediment transport parameters. Ramp slope initiation is favoured by still stands and falls of sea-level, in a setting with high-frequency sea-level fluctuations with amplitude between 20 m and 40 m. Low-frequency and higher amplitude sea-level fluctuations of about 115 m tend to form models with no significant slope development. The impact of antecedent slope on the geometry of ramps is determined by the paleoslope inclination, with flat to subhorizontal paleosurfaces resulting in ramps that mirror the antecedent slope. In contrast, steeper paleosurfaces tend to result in ramps with well-defined slopes. Our models, therefore, show that the ramp profile becomes more influenced by the depth constraints on the carbonate sediment producers than by the geometry of the underlying topography as the inclination of the paleosurface increases. The presented models also show that seagrass-dominated shallow carbonate production tends to result in steep slopes due to the low-transport characteristic imposed by seagrass trapping. This steepness can, however, be altered by the introduction of high transport sediment grains from deeper carbonate producers, which fill the slopes and more distal sections of the ramp profile.
Non-local muscle fatigue effects on muscle strength, power, and endurance in healthy individuals
(2021)
Background
The fatigue of a muscle or muscle group can produce global responses to a variety of systems (i.e., cardiovascular, endocrine, and others). There are also reported strength and endurance impairments of non-exercised muscles following the fatigue of another muscle; however, the literature is inconsistent.
Objective
To examine whether non-local muscle fatigue (NLMF) occurs following the performance of a fatiguing bout of exercise of a different muscle(s).
Design
Systematic review and meta-analysis.
Search and Inclusion
A systematic literature search using a Boolean search strategy was conducted with PubMed, SPORTDiscus, Web of Science, and Google Scholar in April 2020, and was supplemented with additional 'snowballing' searches up to September 2020. To be included in our analysis, studies had to include at least one intentional performance measure (i.e., strength, endurance, or power), which if reduced could be considered evidence of muscle fatigue, and also had to include the implementation of a fatiguing protocol to a location (i.e., limb or limbs) that differed to those for which performance was measured. We excluded studies that measured only mechanistic variables such as electromyographic activity, or spinal/supraspinal excitability. After search and screening, 52 studies were eligible for inclusion including 57 groups of participants (median sample = 11) and a total of 303 participants.
Results
The main multilevel meta-analysis model including all effects sizes (278 across 50 clusters [median = 4, range = 1 to 18 effects per cluster) revealed a trivial point estimate with high precision for the interval estimate [- 0.02 (95% CIs = - 0.14 to 0.09)], yet with substantial heterogeneity (Q((277)) = 642.3, p < 0.01), I-2 = 67.4%). Subgroup and meta-regression analyses showed that NLMF effects were not moderated by study design (between vs. within-participant), homologous vs. heterologous effects, upper or lower body effects, participant training status, sex, age, the time of post-fatigue protocol measurement, or the severity of the fatigue protocol. However, there did appear to be an effect of type of outcome measure where both strength [0.11 (95% CIs = 0.01-0.21)] and power outcomes had trivial effects [- 0.01 (95% CIs = - 0.24 to 0.22)], whereas endurance outcomes showed moderate albeit imprecise effects [- 0.54 (95% CIs = - 0.95 to - 0.14)].
Conclusions
Overall, the findings do not support the existence of a general NLMF effect; however, when examining specific types of performance outcomes, there may be an effect specifically upon endurance-based outcomes (i.e., time to task failure). However, there are relatively fewer studies that have examined endurance effects or mechanisms explaining this possible effect, in addition to fewer studies including women or younger and older participants, and considering causal effects of prior training history through the use of longitudinal intervention study designs. Thus, it seems pertinent that future research on NLMF effects should be redirected towards these still relatively unexplored areas.
Involvement in sport and exercise not only provides participants with health benefits but can be an important aspect of living a meaningful life. The COVID-19 pandemic and the temporary cessation of public life in March/April/May 2020 came with restrictions, which probably also made it difficult, if not impossible, to participate in certain types of sport or exercise. Following the philosophical position that different types of sport and exercise offer different ways of "relating to the world," this study explored (dis)continuity in the type of sport and exercise people practiced during the pandemic-related lockdown, and possible effects on mood. Data from a survey of 601 adult exercisers, collected shortly after the COVID-19 outbreak in Finland, were analyzed. Approximately one third (35%) of the participants changed their "worldmaking" and shifted to "I-Nature"-type activities. We observed worse mood during the pandemic in those who shifted from "I-Me," compared to those who had preferred the "I-Nature" relation already before the pandemic and thus experienced continuity. The clouded mood of those experiencing discontinuity may be the result of a temporary loss of "feeling at home" in their new exercise life-world. However, further empirical investigation must follow, because the observed effect sizes were small.
Mining of metabolite-protein interaction networks facilitates the identification of design principles underlying the regulation of different cellular processes. However, identification and characterization of the regulatory role that metabolites play in interactions with proteins on a genome-scale level remains a pressing task. Based on availability of high-quality metabolite-protein interaction networks and genome-scale metabolic networks, here we propose a supervised machine learning approach, called CIRI that determines whether or not a metabolite is involved in a competitive inhibitory regulatory interaction with an enzyme. First, we show that CIRI outperforms the naive approach based on a structural similarity threshold for a putative competitive inhibitor and the substrates of a metabolic reaction. We also validate the performance of CIRI on several unseen data sets and databases of metabolite-protein interactions not used in the training, and demonstrate that the classifier can be effectively used to predict competitive inhibitory interactions. Finally, we show that CIRI can be employed to refine predictions about metabolite-protein interactions from a recently proposed PROMIS approach that employs metabolomics and proteomics profiles from size exclusion chromatography in E. coli to predict metaboliteprotein interactions. Altogether, CIRI fills a gap in cataloguing metabolite-protein interactions and can be used in directing future machine learning efforts to categorize the regulatory type of these interactions.
We have directly resolved in the present work the interfacial composition during and after the interactions of a saturated atmosphere of oil vapor with soluble surfactant solutions at a planar water/air interface for the first time. Experiments were conducted on interactions of hexane vapor with solutions of alkyltrimethylammonium bromides and sodium dodecyl sulfate to observe the balance between cooperativity and competition of the components at the interface.
In all cases, hexane adsorption was strongly enhanced by the presence of the surfactant, even at bulk surfactant concentrations four orders of magnitude below the critical micelle concentration. Cooperativity of the surfactant adsorption was observed only for sodium dodecyl sulfate at intermediate bulk concentrations, yet for all four systems, competition set in at higher concentrations, as hexane adsorption reduced the surfactant surface excess. The data fully supported the complete removal of hexane from the interface following venting of the system to remove the saturated atmosphere of oil vapor.
These results help to identify future experiments that would elaborate and could explain the cooperativity of surfactant adsorption, such as on cationic surfactants with short alkyl chains and a broader series of anionic surfactants. This work holds relevance for oil recovery applications with foam, where there is a gas phase saturated with oil vapor.
The human language processing mechanism assigns a structure to the incoming materials as they unfold. There is evidence that the parser prefers some attachment types over others; however, theories of sentence processing are still in dispute over the stage at which each source of information contributes to the parsing system. The present study aims to identify the nature of initial parsing decisions during sentence processing through manipulating attachment type and verbs' argument structure. To this end, we designed a self-paced reading task using globally ambiguous constructions in Dutch. The structures included double locative prepositional phrases (PPs) where the first PP could attach both to the verb (high attachment) and the noun preceding it (low attachment). To disambiguate the structures, we presented a visual context in the form of short animation clips prior to each reading task. Furthermore, we manipulated the argument structure of the sentences using 2- and 3-argument verbs. The results showed that parsing decisions were influenced by contextual cues depending on the argument structure of the verb. That is, the visual context overcame the preference for high attachment only in the case of 2-argument verbs, while this preference persisted in structures including 3-argument verbs as represented by longer reading times for the low attachment interpretations. These findings can be taken as evidence that our language processing system actively integrates information from linguistic and non-linguistic sources from the initial stages of analysis to build up meaning. We discuss our findings in light of serial and parallel models of sentence processing.
Recent studies indicated severe decline of insect diversity and abundance across major parts of Central Europe.
Theoretical studies showed that the drivers behind biodiversity loss vary considerably over time. However, these scenarios so far have been insufficiently approved by long-term and large-scale data.
In this study we analysed the temporal trends of butterflies and Zygaenid moths across the federal state of Salzburg, northern Austria, from 1920 to 2019. Our study area covers a large variety of habitats and altitudes.
Various changes of land use and intensification occurred during and shortly before our studied period, with a first wave of habitat destruction starting in the late 19th century, followed by the deterioration of habitat quality since the mid-20th century. We used 59,870 presence-only data of 168 butterfly and burnet moth species.
Each of these species was classified according to ecological characteristics. Break point analyses for non-linear temporal trends in the community composition returned two major time windows.
These time windows coincide with periods characterized by severe habitat destruction and the deterioration of habitat quality due to agricultural intensification. We found significant reductions of the proportion of species requiring specific habitats since 1920 and until today.
We identified additional break points for species requiring high habitat qualities, endangered butterfly species, and sedentary species, particularly after a main break point in the 1960s.
Our findings underline that, apart from habitat destruction, the deterioration of habitat quality is a main driver of biodiversity loss in general.
Therefore, nature conservation should focus on maintaining the highest possible habitat quality.
Nudix hydrolase NUDT19 regulates mitochondrial function and ATP production in murine hepatocytes
(2022)
Changes in intracellular CoA levels are known to contribute to the development of non-alcoholic fatty liver disease (NAFLD) in type 2 diabetes (T2D) in human and rodents. However, the underlying genetic basis is still poorly understood.
Due to their diverse susceptibility towards metabolic diseases, mouse inbred strains have been proven to serve as powerful tools for the identification of novel genetic factors that underlie the patho-physiology of NAFLD and diabetes. Transcriptome analysis of mouse liver samples revealed the nucleoside diphosphate linked moiety X-type motif Nudt19 as novel candidate gene responsible for NAFLD and T2D development. Knockdown (KD) of Nudt19 increased mitochondrial and glycolytic ATP production rates in Hepa 1-6 cells by 41% and 10%, respectively.
The enforced utilization of glutamine or fatty acids as energy substrate reduced uncoupled respiration by 41% and 47%, respectively, in non-target (NT) siRNA transfected cells.
This reduction was prevented upon Nudt19 KD. Furthermore, incubation with palmitate or oleate respectively increased mitochondrial ATP production by 31% and 20%, and uncoupled respiration by 23% and 30% in Nudt19 KD cells, but not in NT cells.
The enhanced fatty acid oxidation in Nudt19 KD cells was accompanied by a 1.3-fold increased abundance of Pdk4.
This study is the first to describe Nudt19 as regulator of hepatic lipid metabolism and potential mediator of NAFLD and T2D development.
Due to the great potential of surface-enhanced Raman scattering (SERS) as local vibrational probe of lipid-nanostructure interaction in lipid bilayers, it is important to characterize these interactions in detail.
The interpretation of SERS data of lipids in living cells requires an understanding of how the molecules interact with gold nanostructures and how intermolecular interactions influence the proximity and contact between lipids and nanoparticles.
Ceramide, a sphingolipid that acts as important structural component and regulator of biological function, therefore of interest to probing, lacks a phosphocholine head group that is common to many lipids used in liposome models.
SERS spectra of liposomes of a mixture of ceramide, phosphatidic acid, and phosphatidylcholine, as well as of pure ceramide and of the phospholipid mixture are reported.
Distinct groups of SERS spectra represent varied contributions of the choline, sphingosine, and phosphate head groups and the structures of the acyl chains. Spectral bands related to the state of order of the membrane and moreover to the amide function of the sphingosine head groups indicate that the gold nanoparticles interact with molecules involved in different intermolecular relations.
While cryogenic electron microscopy shows the formation of bilayer liposomes in all preparations, pure ceramide was found to also form supramolecular, concentric stacked and densely packed lamellar, nonliposomal structures. That the formation of such supramolecular assemblies supports the intermolecular interactions of ceramide is indicated by the SERS data.
The unique spectral features that are assigned to the ceramide-containing lipid model systems here enable an identification of these molecules in biological systems and allow us to obtain information on their structure and interaction by SERS.
Describing the heterogeneous structure of forests is often challenging.
One possibility is to analyze forest biomass in different plots and to derive plot-based frequency distributions.
However, these frequency distributions depend on the plot size and thus are scale dependent.
This study provides insights about transferring them between scales. Understanding the effects of scale on distributions of biomass is particularly important for comparing information from different sources such as inventories, remote sensing and modeling, all of which can operate at different spatial resolutions. Reliable methods to compare results of vegetation models at a grid scale with field data collected at smaller scales are still missing.
The scaling of biomass and variables, which determine the forest biomass, was investigated for a tropical forest in Panama. Based on field inventory data from Barro Colorado Island, spanning 50 ha over 30 years, the distributions of aboveground biomass, biomass gain and mortality were derived at different spatial resolutions, ranging from 10 to 100 m. Methods for fitting parametric distribution functions were compared.
Further, it was tested under which assumptions about the distributions a simple stochastic simulation forest model could best reproduce observed biomass distributions at all scales. Also, an analytical forest model for calculating biomass distributions at equilibrium and assuming mortality as a white shot noise process was tested.
Scaling exponents of about 0.47 were found for the standard deviations of the biomass and gain distributions, while mortality showed a different scaling relationship with an exponent of 0.3. Lognormal and gamma distribution functions fitted with the moment matching estimation method allowed for consistent parameter transfers between scales. Both forest models (stochastic simulation and analytical solution) were able to reproduce observed biomass distributions across scales, when combined with the derived scaling relationships.
The study demonstrates a way of how to approach the scaling problem in model-data comparisons by providing a transfer relationship. Further research is needed for a better understanding of the mechanisms that shape the frequency distributions at the different scales.
Clusty is a new open source toolbox dedicated to earthquake clustering based on waveforms recorded across a network of seismic stations. Its main application is the study of active faults and the detection and characterization of faults and fault networks. By using a density-based clustering approach, earthquakes pertaining to a common fault can be recognized even over long fault segments, and the first-order geometry and extent of active faults can be inferred. Clusty implements multiple techniques to compute a waveform based network similarity from maximum cross-correlation coefficients at multiple stations. The clustering procedure is designed to be transparent and parameters can be easily tuned. It is supported by a number of analysis visualization tools which help to assess the homogeneity within each cluster and the differences among distinct clusters. The toolbox returns graphical representations of the results. A list of representative events and stacked waveforms facilitate further analyses like moment tensor inversion. Results obtained in various frequency bands can be combined to account for large magnitude ranges. Thanks to the simple configuration, the toolbox is easily adaptable to new data sets and to large magnitude ranges. To show the potential of our new toolbox, we apply Clusty to the aftershock sequence of the M-w 6.9 25 October 2018 Zakynthos (Greece) Earthquake. Thanks to the complex tectonic setting at the western termination of the Hellenic Subduction System where multiple faults and faulting styles operate simultaneously, the Zakynthos data set provides an ideal case-study for our clustering analysis toolbox. Our results support the activation of several faults and provide insight into the geometry of faults or fault segments. We identify two large thrust faulting clusters in the vicinity of the main shock and multiple strike-slip clusters to the east, west and south of these clusters. Despite its location within the largest thrust cluster, the main shock does not show a high waveform similarity to any of the clusters. This is consistent with the results of other studies suggesting a complex failure mechanism for the main shock. We propose the existence of conjugated strike-slip faults in the south of the study area. Our waveform similarity based clustering toolbox is able to reveal distinct event clusters which cannot be discriminated based on locations and/or timing only. Additionally, the clustering results allows distinction between fault and auxiliary planes of focal mechanisms and to associate them to known active faults.
Scanning manufacturing parameters determining the residual stress state in LPBF IN718 small parts
(2021)
The influence of scan strategy on the residual stress (RS) state of an as-built IN718 alloy produced by means of laser powder bed fusion (LPBF) is investigated. Two scan vector rotations (90 degrees-alternation and 67 degrees-rotation), each produced following two different scan vector lengths (long and short), are used to manufacture four rectangular prisms. Neutron diffraction (ND) and laboratory X-ray diffraction (XRD) techniques are used to map the bulk and surface RS state, respectively. The distortion induced upon removal from the baseplate is measured via profilometry. XRD measurements show that the two long scan vector strategies lead to higher RS when compared with the equivalent short scan vector strategies. Also, the 67 degrees-rotation strategies generate lower RS than their 90 degrees-alternation counterparts. Due to the lack of reliable stress-free d0 references, the ND results are analyzed using von Mises stress. In general, ND results show significant RS spatial non-uniformity. A comparison between ND and distortion results indicates that the RS component parallel to the building direction (Z-axis) has a predominant role in the Z-displacement. The use of a stress balance scheme allows to discuss the d0 variability along the length of the specimens, as well as examine the absolute RS state.
Impact of Late Pleistocene climate variability on paleo-erosion rates in the western Himalaya
(2022)
It has been proposed that at short timescales of 10(2)-10(5) yr, climatic variability can explain variations in sediment flux, but in orogens with pronounced climatic gradients rate changes caused by the oscillating efficiency in rainfall, runoff, and/or sediment transport and deposition are still not well-constrained.
To explore landscape responses under variable climatic forcing, we evaluate time windows of prevailing sediment aggradation and related paleo-erosion rates from the southern flanks of the Dhauladhar Range in the western Himalaya.
We compare past and present Be-10-derived erosion rates of well-dated Late Pleistocene fluvial landforms and modern river sediments and reconstruct the sediment aggradation and incision history based on new luminescence data.
Our results document significant variations in erosion rates ranging from 0.1 to 3.4 mm/yr over the Late Pleistocene.
We find that, during times of weak monsoon intensity, the moderately steep areas (hillslope angles of 27 +/- 13 degrees) erode at lower rates of 0.1-0.4 mm/yr compared to steeper (>40 degrees) crestal regions of the Dhauladhar Range that erode at 0.8-1.3 mm/yr.
In contrast, during several millennia of stronger monsoon intensity, both the moderately steep and high slope areas record higher erosion rates (>1-3.4 mm/yr). Lithological clast-count analysis shows that this increase of erosion is focused in the moderately steep areas, where Lesser Himalayan rocks are exposed.
Our data thus highlight the highly non-linear response of climatic forcing on landscape evolution and suggest complex depositional processes and sedimentary signals in downstream areas. (C) 2021 Elsevier B.V. All rights reserved.
Media discourse about Islamist terrorism can be understood as an important source for the construction of meaning and reality. This chapter aims to explore the different meanings of threat constituted by the media discourse about Islamist terrorism. Additionally, it seeks to shed light on the role of anti-Muslim stereotypes and racism in the discursive construction of meaning and knowledge. Therefore, this study examines the discourse on three terrorist events from the years 2015 and 2016 gathered from four major German newspapers. By applying the Sociology of Knowledge Approach to Discourse (SKAD), the findings reveal three interpretive schemes about threats associated with Islamist terrorism and their different references to anti-Muslim stereotypes and racism.
The current study examined the impact of the Good Behavior Game (GBG) on the academic engagement (AE) and disruptive behavior (DB) of at-risk students' in a German inclusive primary school sample using behavioral progress monitoring.
A multiple baseline design across participants was employed to evaluate the effects of the GBG on 35 primary school students in seven classrooms from grade 1 to 3 (M-age = 8.01 years, SDage = 0.81 years).
The implementation of the GBG was randomly staggered by 2 weeks across classrooms. Teacher-completed Direct Behavior Rating (DBR) was applied to measure AE and DB. We used piecewise regression and a multilevel extension to estimate the individual case-specific treatment effects as well as the generalized effects across cases.
Piecewise regressions for each case showed significant immediate treatment effects for the majority of participants (82.86%) for one or both outcome measures.
The multilevel approach revealed that the GBG improved at-risk students' classroom behaviors generally with a significant immediate treatment effect across cases (for AE, B = 0.74, p < 0.001; for DB, B = -1.29, p < 0.001).
The moderation between intervention effectiveness and teacher ratings of students' risks for externalizing psychosocial problems was significant for DB (B = -0.07, p = 0.047) but not for AE.
Findings are consistent with previous studies indicating that the GBG is an appropriate classroom-based intervention for at-risk students and expand the literature regarding differential effects for affected students.
In addition, the study supports the relevance of behavioral progress monitoring and data-based decision-making in inclusive schools in order to evaluate the effectiveness of the GBG and, if necessary, to modify the intervention for individual students or the whole group.
The efficient use of natural resources is considered a necessary condition for their sustainable use. Extending the lifetime of products and using resources circularly are two popular strategies to increase the efficiency of resource use.
Both strategies are usually assumed to contribute to the eco-efficiency of resource use independently.
We argue that a move to a circular economy creates opportunity costs for consumers holding on to their products, due to the resource embedded in the product. Assuming rational consumers, we develop a model that determines optimal replacement times for products subject to minimizing average costs over time.
We find that in a perfectly circular economy, consumers are incentivized to discard their products more quickly than in a perfectly linear economy.
A direct consequence of our finding is that extending product use is in direct conflict with closing resource loops in the circular economy.
We identify the salvage value of discarded products and technical progress as two factors that determine the impact that closing resource loops has on the duration of product use. The article highlights the risk that closing resource loops and moving to a more circular economy incentivizes more unsustainable behavior.
Bastnsite [REE(CO3)F] is the main mineral of REE ore deposits in carbonatites. Synthetic bastnsite-like compounds were precipitated from aqueous solutions by many different methods, but previous attempts to model magmatic crystallization of bastnsite from hydrous calciocarbonatite melts were unsuccessful.
Here we present the first experimental evidence that bastnsite and two other REE carbonates, burbankite, and lukechangite, can crystallize from carbonatite melt in the synthetic system La(CO3)F-CaCO3-Na2CO3 at temperatures between 580 and 850 degrees C and a pressure 100 MPa.
The experiments on starting mixtures of reagent-grade CaCO3, Na2CO3, La-2(CO3)(3), and LaF3 were carried out in cold-seal rapid-quench pressure vessels.
The studied system is an isobaric pseudoternary join of a quinary system where CO2 and fluorides act as independent components. Liquidus phases in the run products are calcite, nyerereite, Na carbonate, bastnsite, burbankite solid solution (Na,Ca)(3)(Ca,La)(3)(CO3)(5), and lukechangite Na3La2(CO3)(4)F. Calcite and bastnite form a eutectic in the boundary join La(CO3)F-CaCO3 at 780 +/- 20 degrees C and 58 wt% La(CO3)F. Phase equilibria in the boundary join La(CO3)F-Na2CO3 are complicated by peritectic reaction between Ca-free end-member of burbankite solid solution petersenite (Pet) and lukechangite (Luk) with liquid (L): Na4La2(CO3)(5) (Pet) + NaF (L) = Na3La2(CO3)(4)F (Luk) + Na2CO3 (Nc).
The right-hand-side assemblage becomes stable below 600 +/- 20 degrees C. In ternary mixtures, bastnsite (Bst), burbankite (Bur), and calcite (Cc) are involved in another peritectic reaction: 2La(CO3)F (Bst) + CaCO3 (Cc) + 2Na(2)CO(3) (L) = Na2CaLa2(CO3)(5) (Bur) + 2NaF (L). Burbankite in equilibrium with calcite replaces bastnsite below 730 +/- 20 degrees C. Stable solidus assemblages in the pseudoternary system are: basnsite-burbankite-fluorite-calcite, basnasite-burbankite-fluorite-lukechangite, bastnsite-burbankite-lukechangite, burbankite-lukechangite-nyerereitecalcite, and burbankite-lukechangite-nyerereite-natrite. Addition of 10 wt% Ca-3(PO4)(2) to one of the ternary mixtures resulted in massive crystallization of La-bearing apatite and monazite and complete disappearance of bastnsite and burbankite.
Our results confirm that REE-bearing phosphates are much more stable than carbonates and fluorocarbonates.
Therefore, primary crystallization of the latter from common carbonatite magmas is unlikely. Possible exceptions are carbonatites at Mountain Pass that are characterized by very low P2O5 concentrations (usually at or below 0.5 wt%) and extremely high REE contents in the order of a few weight percent or more. In other carbonatites, bastnsite and burbankite probably crystallized from highly concentrated alkaline carbonate-chloride brines that were found in melt inclusions and are thought to be responsible for widespread fenitization around carbonatite bodies.
Objective:
Little attention has been given to the relationship between cyber polyvictimization and academic outcomes (e.g., classroom misconduct, school readiness, academic performance, absenteeism, school behavioral problems), and the factors, such as parent social support, that buffer against the negative outcomes associated with experiencing multiple forms of victimization. Addressing gaps in the literature by including a longitudinal design and objective assessments of academic outcomes, the present study examined the moderating effect of parent social support in the association between cyber polyvictimization and academic outcomes over one and a half years later.
Method:
Participants were 371 8th graders (50% female) from middle schools in the United States, who completed questionnaires on offline and cyber polyvictimization and parent social support during the 7th grade. Teachers completed questionnaires on students' classroom misconduct and school readiness during 7th and 8th grade. School records were used to determine absenteeism, academic performance, and school behavioral problems (i.e., referrals, in-school suspension, out-of-school suspension) during 7th and 8th grade.
Results:
Findings revealed that 7th grade cyber polyvictimization was related positively to 8th grade classroom misconduct, absenteeism, and school behavioral problems, while it was negatively associated with 8th grade academic performance and school readiness. Parent social support moderated the associations between cyber polyvictimization and school readiness, academic performance, and absenteeism. Conclusion: The results highlight the importance of intervening in adolescents' experience of cyber polyvictimization to reduce negative academic outcomes.
Fractional Brownian motion, a Gaussian non-Markovian self-similar process with stationary long-correlated increments, has been identified to give rise to the anomalous diffusion behavior in a great variety of physical systems. The correlation and diffusion properties of this random motion are fully characterized by its index of self-similarity or the Hurst exponent.
However, recent single-particle tracking experiments in biological cells revealed highly complicated anomalous diffusion phenomena that cannot be attributed to a class of self-similar random processes.
Inspired by these observations, we here study the process that preserves the properties of the fractional Brownian motion at a single trajectory level; however, the Hurst index randomly changes from trajectory to trajectory.
We provide a general mathematical framework for analytical, numerical, and statistical analysis of the fractional Brownian motion with the random Hurst exponent.
The explicit formulas for probability density function, mean-squared displacement, and autocovariance function of the increments are presented for three generic distributions of the Hurst exponent, namely, two-point, uniform, and beta distributions.
The important features of the process studied here are accelerating diffusion and persistence transition, which we demonstrate analytically and numerically.
The chaotic spatio-temporal electrical activity during life-threatening cardiac arrhythmias like ventricular fibrillation is governed by the dynamics of vortex-like spiral or scroll waves. The organizing centers of these waves are called wave tips (2D) or filaments (3D) and they play a key role in understanding and controlling the complex and chaotic electrical dynamics. Therefore, in many experimental and numerical setups it is required to detect the tips of the observed spiral waves. Most of the currently used methods significantly suffer from the influence of noise and are often adjusted to a specific situation (e.g. a specific numerical cardiac cell model). In this study, we use a specific type of deep neural networks (UNet), for detecting spiral wave tips and show that this approach is robust against the influence of intermediate noise levels. Furthermore, we demonstrate that if the UNet is trained with a pool of numerical cell models, spiral wave tips in unknown cell models can also be detected reliably, suggesting that the UNet can in some sense learn the concept of spiral wave tips in a general way, and thus could also be used in experimental situations in the future (ex-vivo, cell-culture or optogenetic experiments).
Background & aims:
This study aimed to describe the association of healthy eating literacy (HEL) with energy, nutrients, and food consumption in young women who had normal and lean weight at a Japanese university, considering their resident status.
Methods:
Cross-sectional data from the Ochanomizu Health Study were used in this study. Participants answered a self-administered, two-part, anonymous survey in 2018 and 2019.
A total of 203 female undergraduate students with lean and normal body mass index (BMI) were included in the analysis. Single and stepwise multiple linear regression analysis was used to examine the association of HEL and resident status with healthy food consumption, such as vegetables, fish, and shellfish.
The dependent variables were HEL and resident status, and the covariates were age, BMI, and the total metabolic equivalents.
Results:
The median (25th and 75th percentiles) age, BMI, and total HEL score were 20 (19, 21) years, 20.2 (18.9, 21.3) kg/m 2, and 18 (16, 20), respectively.
Resident status and HEL were independently associated with vegetables, fish, and shellfish intake.
Participants who had higher total HEL scores and lived in their family home consumed significantly more vegetables (b = 0.17 and-0.34, p < 0.05) and fish and shellfish (b = 0.24,-0.28, p < 0.001).
Conclusion:
This study provides an insight into the association between HEL and dietary consumption in young women with normal and lean BMI.
Cosmic-ray (CR) diffusion is the result of the interaction of such charged particles against magnetic fluctuations. These fluctuations originate from large-scale turbulence cascading toward smaller spatial scales, decomposed into three different modes, as described by magnetohydrodynamics (MHD) theory.
As a consequence, the description of particle diffusion strongly depends on the model describing the injected turbulence.
Moreover, the amount of energy assigned to each of the three modes is, in general, not equally divided, which implies that diffusion properties might be different from one region to another.
Here, motivated by the detection of different MHD modes inside the Cygnus-X star-forming region, we study the 3D transport of CRs injected by two prominent sources within a two-zone model that represents the distribution of the modes.
Then, by convolving the propagated CR distribution with the neutral gas, we are able to explain the 𝛾-ray diffuse emission in the region, observed by the Fermi-LAT and HAWC Collaborations.
Such a result represents an important step in the long-standing problem of connecting the CR observables with the microphysics of particle transport.
Organic-inorganic composite materials with tailored properties can be designed in the lab through bioinspired approaches.
In this context, we exploited the particle-based crystallisation process of calcium sulfate, a technologically important mineral, to hybridise inorganic and organic matter.
We identified and synthesised an organic polymer showing strong affinity to bind to the surfaces of mineral precursors as well as intrinsic tendency to self-organise. Subsequently, polymer-coated building units were allowed to self-assemble via oriented attachment, aggregation and phase transformation, which produced ordered superstructures where the organic polymer is intercalated between the subunits and surrounds the hybrid core as a shell.
This specific architecture across multiple length scales leads to unique mechanical properties, comparable to those of natural biominerals.
Thus, our results devise a straightforward pathway to prepare organic-inorganic hybrid structures via bottom-up self-assembly processes innate to the crystallisation of the inorganic phase.
This approach can likely be transferred to other inorganic minerals, affording next-generation materials for applications in the construction sector, biomedicine and beyond.
Evolutionary reduction of adult body size (miniaturization) has profound consequences for organismal biology and is an important subject of evolutionary research. Based on two individuals we describe a new, extremely miniaturized chameleon, which may be the world's smallest reptile species. The male holotype of Brookesia nana sp. nov. has a snout-vent length of 13.5 mm (total length 21.6 mm) and has large, apparently fully developed hemipenes, making it apparently the smallest mature male amniote ever recorded. The female paratype measures 19.2 mm snout-vent length (total length 28.9 mm) and a micro-CT scan revealed developing eggs in the body cavity, likewise indicating sexual maturity. The new chameleon is only known from a degraded montane rainforest in northern Madagascar and might be threatened by extinction. Molecular phylogenetic analyses place it as sister to B. karchei, the largest species in the clade of miniaturized Brookesia species, for which we resurrect Evoluticauda Angel, 1942 as subgenus name. The genetic divergence of B. nana sp. nov. is rather strong (9.914.9% to all other Evoluticauda species in the 16S rRNA gene). A comparative study of genital length in Malagasy chameleons revealed a tendency for the smallest chameleons to have the relatively largest hemipenes, which might be a consequence of a reversed sexual size dimorphism with males substantially smaller than females in the smallest species. The miniaturized males may need larger hemipenes to enable a better mechanical fit with female genitals during copulation. Comprehensive studies of female genitalia are needed to test this hypothesis and to better understand the evolution of genitalia in reptiles.
The desiccation of the Aral Sea represents one of the largest human-made environmental regional disasters. The salt- and toxin-enriched dried-out basin provides a natural laboratory for studying ecosystem functioning and rhizosphere assembly under extreme anthropogenic conditions.
Here, we investigated the prokaryotic rhizosphere communities of the native pioneer plant Suaeda acuminata (C.A.Mey.) Moq. in comparison to bulk soil across a gradient of desiccation (5, 10, and 40 years) by metagenome and amplicon sequencing combined with quantitative PCR (qPCR) analyses. The rhizosphere effect was evident due to significantly higher bacterial abundances but less diversity in the rhizosphere compared to bulk soil. Interestingly, in the highest salinity (5 years of desiccation), rhizosphere functions were mainly provided by archaeal communities.
Along the desiccation gradient, we observed a significant change in the rhizosphere microbiota, which was reflected by (i) a decreasing archaeon-bacterium ratio, (ii) replacement of halophilic archaea by specific plant-associated bacteria, i.e., Alphaproteobacteria and Actinobacteria, and (iii) an adaptation of specific, potentially plant-beneficial biosynthetic pathways.
In general, both bacteria and archaea were found to be involved in carbon cycling and fixation, as well as methane and nitrogen metabolism.
Analysis of metagenome-assembled genomes (MAGs) showed specific signatures for production of osmoprotectants, assimilatory nitrate reduction, and transport system induction.
Our results provide evidence that rhizosphere assembly by cofiltering specific taxa with distinct traits is a mechanism which allows plants to thrive under extreme conditions. Overall, our findings highlight a function-based rhizosphere assembly, the importance of plant-microbe interactions in salinated soils, and their exploitation potential for ecosystem restoration approaches.IMPORTANCE
The desertification of the Aral Sea basin in Uzbekistan and Kazakhstan represents one of the most serious anthropogenic environmental disasters of the last century. Since the 1960s, the world's fourth-largest inland body of water has been constantly shrinking, which has resulted in an extreme increase of salinity accompanied by accumulation of many hazardous and carcinogenic substances, as well as heavy metals, in the dried-out basin.
Here, we investigated bacterial and archaeal communities in the rhizosphere of pioneer plants by combining classic molecular methods with amplicon sequencing as well as metagenomics for functional insights.
By implementing a desiccation gradient, we observed (i) remarkable differences in the archaeon-bacterium ratio of plant rhizosphere samples, (ii) replacement of archaeal indicator taxa during succession, and (iii) the presence of specific, potentially plant-beneficial biosynthetic pathways in archaea present during the early stages.
In addition, our results provide hitherto-undescribed insights into the functional redundancy between plant-associated archaea and bacteria.
The desertification of the Aral Sea basin in Uzbekistan and Kazakhstan represents one of the most serious anthropogenic environmental disasters of the last century.
Since the 1960s, the world's fourth-largest inland body of water has been constantly shrinking, which has resulted in an extreme increase of salinity accompanied by accumulation of many hazardous and carcinogenic substances, as well as heavy metals, in the dried-out basin.
From June to August 2021, we deployed a dense seismic nodal network across the Hengill geothermal area in southwest Iceland to image and characterize faults and high-temperature zones at high resolution.
The nodal network comprised 498 geophone nodes spread across the northern Nesjavellir and southern Hverahlio geothermal fields and was complemented by an existing permanent and temporary backbone seismic network of a total of 44 short-period and broadband stations.
In addition, we recorded distributed acoustic sensing data along two fiber optic telecommunication cables near the Nesjavellir geothermal power plant with commercial interrogators.
During the time of deployment, a vibroseis survey took place around the Nesjavellir power plant.
Here, we describe the network and the recorded datasets.
Furthermore, we showsome initial results that indicate a high data quality and highlight the potential of the seismic records for various follow up studies, such as high-resolution event location to delineate faults and body- and surface-wave tomographies to image the subsurface velocity structure in great detail.
Lakes act as important sinks for inorganic and organic sediment components. However, investigations of sedimentary carbon budgets within glacial lakes are currently absent from Arctic Siberia. The aim of this paper is to provide the first reconstruction of accumulation rates, sediment and carbon budgets from a lacustrine sediment core from Lake Rauchuagytgyn, Chukotka (Arctic Siberia). We combined multiple sediment biogeochemical and sedimentological parameters from a radiocarbon-dated 6.5m sediment core with lake basin hydroacoustic data to derive sediment stratigraphy, sediment volumes and infill budgets. Our results distinguished three principal sediment and carbon accumulation regimes that could be identified across all measured environmental proxies including early Marine Isotope Stage 2 (MIS2) (ca. 29-23.4 ka cal BP), mid-MIS2-early MIS1 (ca. 23.4-11.69 ka cal BP) and the Holocene (ca. 11.69-present). Estimated organic carbon accumulation rates (OCARs) were higher within Holocene sediments (average 3.53 gOCm(-2) a(-1)) than Pleistocene sediments (average 1.08 gOCm(-2) a(-1)) and are similar to those calculated for boreal lakes from Quebec and Finland and Lake Baikal but significantly lower than Siberian thermokarst lakes and Alberta glacial lakes. Using a bootstrapping approach, we estimated the total organic carbon pool to be 0.26 +/- 0.02 Mt and a total sediment pool of 25.7 +/- 1.71 Mt within a hydroacoustically derived sediment volume of ca. 32 990 557m(3). The total organic carbon pool is substantially smaller than Alaskan yedoma, thermokarst lake sediments and Alberta glacial lakes but shares similarities with Finnish boreal lakes. Temporal variability in sediment and carbon accumulation dynamics at Lake Rauchuagytgyn is controlled predominantly by palaeoclimate variation that regulates lake ice-cover dynamics and catchment glacial, fluvial and permafrost processes through time. These processes, in turn, affect catchment and within-lake primary productivity as well as catchment soil development. Spatial differences compared to other lake systems at a trans-regional scale likely relate to the high-latitude, mountainous location of Lake Rauchuagytgyn.
Martian atmospheric spectral end-members retrieval from ExoMars Thermal Infrared (TIRVIM) data
(2022)
Key knowledge about planetary composition can be recovered from the study of thermal infrared spectral range datasets.
This range has a huge diagnostic potential because it contains diagnostic absorptions from a planetary surface and atmosphere. The main goal of this study is to process and interpret the dataset from the Thermal Infrared channel (TIRVIM) which is part of the Atmospheric Chemistry Suite of the ExoMars2016 Trace Gas Orbiter mission to find and characterize dust and water ice clouds in the atmosphere.
The method employed here is based on the application of principal component analysis and target transformation techniques to extract the independent variable components present in the analyzed dataset. Spectral shapes of both atmospheric dust and water ice aerosols have been recovered from the analysis of TIRVIM data.
The comparison between our results with those previously obtained on Thermal Emission Spectrometer (TES) data and with previous analysis on TIRVIM data, validates the methodology here applied, showing that it allows to correctly recover the atmospheric spectral endmembers present in the TIRVIM data.
Moreover, comparison with atmospheric retrievals on PFS, TES and IRIS data, allowed us to assess the temporal stability and homogeneity of dust and water ice components in the Martian atmosphere over a time period of almost 50 years.
Heat shock factor HSFA2 fine-tunes resetting of thermomemory via plastidic metalloprotease FtsH6
(2022)
The transcription factor HSFA2 fine-tunes a balance between prolongation and resetting of thermomemory in Arabidopsis via the regulation of both memory-supporting and memory-resetting genes.
Plants 'memorize' stressful events and protect themselves from future, often more severe, stresses. To maximize growth after stress, plants 'reset' or 'forget' memories of stressful situations, which requires an intricate balance between stress memory formation and the degree of forgetfulness.
HEAT SHOCK PROTEIN 21 (HSP21) encodes a small heat shock protein in plastids of Arabidopsis thaliana. HSP21 functions as a key component of thermomemory, which requires a sustained elevated level of HSP21 during recovery from heat stress. A heat-induced metalloprotease, filamentation temperature-sensitive H6 (FtsH6), degrades HSP21 to its pre-stress abundance, thereby resetting memory during the recovery phase. The transcription factor heat shock factor A2 (HSFA2) activates downstream genes essential for mounting thermomemory, acting as a positive regulator in the process.
Here, using a yeast one-hybrid screen, we identify HSFA2 as an upstream transactivator of the resetting element FtsH6. Constitutive and inducible overexpression of HSFA2 increases expression of FtsH6, whereas it is drastically reduced in the hsfa2 knockout mutant. Chromatin immunoprecipitation reveals in planta binding of HSFA2 to the FtsH6 promoter. Importantly, overexpression of HSFA2 improves thermomemory more profoundly in ftsh6 than wild-type plants.
Thus, by activating both memory-supporting and memory-resetting genes, HSFA2 acts as a cellular homeostasis factor during thermomemory.
As cultural diversity is increasing around the globe, a more nuanced understanding of the cultural diversity climate in classroom settings is needed, including how its different aspects relate to student outcomes. We developed the Classroom Cultural Diversity Climate Scale (CCDCS), integrating theory and research from social psychology and multicultural education and including novel facets like polyculturalism, which has not been studied in the school context before. We then studied associations with intergroup relations, socio-emotional adjustment, and school achievement among students of immigrant and non-immigrant background at the individual and classroom levels. The scale includes six subscales in the two broad dimensions of equality and inclusion: contact and cooperation, (un)equal treatment, and color-evasion, and cultural pluralism: heritage and intercultural learning, critical consciousness, and polyculturalism. Using data from 1,335 secondary school students in Germany (Mage = 14.7; 51% male; 51% immigrant background), the scale demonstrated measurement invariance by immigrant background, gender, and school track, and reliability at individual and classroom levels. A more positive diversity climate, with better intercultural relations (equality and inclusion) and more opportunities to learn about cultural diversity (cultural pluralism), was associated with more positive student outcomes. Interestingly, polyculturalism was not associated with negative effects observed for other facets of cultural pluralism. Relations for different climate aspects also varied by outcome and students' immigrant background. This underscores the importance of a nuanced perspective when evaluating different approaches to cultural diversity in context.
Aim: Study aim was to investigate the effects of therapeutic phlebotomy on ambulatory blood pressure in patients with grade 1 hypertension.
Methods: In this randomized-controlled intervention study, patients with unmedicated hypertension grade 1 were randomized into an intervention group (phlebotomy group; 500 mL bloodletting at baseline and after 6 weeks) and a control group (waiting list) and followed up for 8 weeks. Primary endpoint was the 24-h ambulatory mean arterial pressure between the intervention and control groups after 8 weeks. Secondary outcome parameters included ambulatory/resting systolic/diastolic blood pressure, heart rate, and selected laboratory parameters (e.g., hemoglobin, hematocrit, erythrocytes, and ferritin). Resting systolic/diastolic blood pressure/heart rate and blood count were also assessed at 6 weeks before the second phlebotomy to ensure safety. A per-protocol analysis was performed.
Results: Fifty-three hypertension participants (56.7 +/- 10.5 years) were included in the analysis (n = 25 intervention group, n = 28 control group). The ambulatory measured mean arterial pressure decreased by -1.12 +/- 5.16mmHg in the intervention group and increased by 0.43 +/- 3.82mmHg in the control group (between-group difference: -1.55 +/- 4.46, p = 0.22). Hemoglobin, hematocrit, erythrocytes, and ferritin showed more pronounced reductions in the intervention group in comparison with the control group, with significant between-group differences. Subgroup analysis showed trends regarding the effects on different groups classified by serum ferritin concentration, body mass index, age, and sex. Two adverse events (AEs) (anemia and dizziness) occurred in association with the phlebotomy, but no serious AEs.
Conclusions: Study results showed that therapeutic phlebotomy resulted in only minimal reductions of 24-h ambulatory blood pressure measurement values in patients with unmedicated grade 1 hypertension. Further high-quality clinical studies are warranted, as this finding contradicts the results of other studies.
The early exhumation history of the Tauern Window in the European Eastern Alps and its surface expression is poorly dated and quantified, partly because thermochronological and provenance information are sparse from the Upper Austrian Northern Alpine Foreland Basin. For the first time, we combine a single-grain double-dating approach (Apatite Fission Track and U-Pb dating) with trace-element geochemistry analysis on the same apatites to reconstruct the provenance and exhumation history of the late Oligocene/early Miocene Eastern Alps. The results from 22 samples from the Chattian to Burdigalian sedimentary infill of the Upper Austrian Northern Alpine Foreland Basin were integrated with a 3D seismic-reflection data set and published stratigraphic reports. Our highly discriminative data set indicates an increasing proportion of apatites (from 6% to 23%) with Sr/Y values <0.1 up-section and an increasing amount of apatites (from 24% to 38%) containing >1,000 ppm light rare-earth elements from Chattian to Burdigalian time. The number of U-Pb ages with acceptable uncertainties increases from 40% to 59% up-section, with mostly late Variscan/Permian ages, while an increasing number of grains (10%-27%) have Eocene or younger apatite fission track cooling ages. The changes in the apatite trace-element geochemistry and U-Pb data mirror increased sediment input from an >= upper amphibolite-facies metamorphic source of late Variscan/Permian age - probably the otztal-Bundschuh nappe system - accompanied by increasing exhumation rates indicated by decreasing apatite fission track lag times. We attribute these changes to the surface response to upright folding and doming in the Penninic units of the future Tauern Window starting at 29-27 Ma. This early period of exhumation (0.3-0.6 mm/a) is triggered by early Adriatic indentation along the Giudicarie Fault System.
The history of the so-called “New Jewish School” in music began in 1908 in St. Petersburg with the founding of the Society for Jewish Folk Music by students from the St. Petersburg Conservatory. The end of this movement came with the invasion of Austria by Nazi Germany in 1938, and the dissolution of the Vienna Society for the Promotion of Jewish Music later that year.
The fascinating and dramatic history of the New Jewish School is the subject of this monograph, which summarizes the author’s years of intensive international archival research. While many other national schools of music – such as the Russian, Czech or Hungarian – were able to develop freely and establish themselves in a favorable cultural environment, the Jewish school was violently suppressed. The reconstruction of its historical development in Russia and, after 1917, increasingly in other Eastern and Central European countries was first presented in German in 2004 and has since served as the basis for rediscovery of the valuable, highly original repertoire of New Jewish School composers. For this English-language publication, the entire book has been thoroughly revised and richly supplemented with extensive additional texts and materials.
The years 1953 through the 1970s in the Soviet Union have been called the era of the “Jews of silence.” And yet through various types of musical activities, certain parts of the Jewish population in the USSR were able to maintain a collective cultural identity in the public sphere. Captured as a musical community, this collectivity also extended to non-Jewish composers, musicians, and audiences. As such it thematicized, performed, represented, and received Jewishness, through Yiddish theater and songs, art music, and popular music. Concerts and works conceived for the Soviet stages demonstrate that Jewishness mattered, with music taking on new symbolism and becoming imbued with new meaning. This chapter focuses on the presence (and absence) of Jewish music in the public sphere, specifically in the concert hall and other stages in the post-Stalinist Soviet Union.
We investigate the failed partial eruption of a filament system in NOAA AR 12104 on 2014 July 5, using multiwavelength EUV, magnetogram, and H alpha observations, as well as magnetic field modeling. The filament system consists of two almost co-spatial segments with different end points, both resembling a C shape. Following an ejection and a precursor flare related to flux cancellation, only the upper segment rises and then displays a prominent twisted structure, while rolling over toward its footpoints. The lower segment remains undisturbed, indicating that the system possesses a double-decker structure. The erupted segment ends up with a reverse-C shape, with material draining toward its footpoints, while losing its twist. Using the flux rope insertion method, we construct a model of the source region that qualitatively reproduces key elements of the observed evolution. At the eruption onset, the model consists of a flux rope atop a flux bundle with negligible twist, which is consistent with the observational interpretation that the filament possesses a double-decker structure. The flux rope reaches the critical height of the torus instability during its initial relaxation, while the lower flux bundle remains in stable equilibrium. The eruption terminates when the flux rope reaches a dome-shaped quasi-separatrix layer that is reminiscent of a magnetic fan surface, although no magnetic null is found. The flux rope is destroyed by reconnection with the confining overlying flux above the dome, transferring its twist in the process.
Natural gas can be temporarily stored in a variety of underground facilities, such as depleted gas and oil fields, natural aquifers and caverns in salt rocks. Being extensively monitored during operations, these systems provide a favourable opportunity to investigate how pressure varies in time and space and possibly induces/triggers earthquakes on nearby faults. Elaborate and detailed numerical modelling techniques are often applied to study gas reservoirs. Here we show the possibilities and discuss the limitations of a flexible and easily formulated tool that can be straightforwardly applied to simulate temporal pore-pressure variations and study the relation with recorded microseismic events. We use the software POEL (POroELastic diffusion and deformation) which computes the poroelastic response to fluid injection/extraction in a horizontally layered poroelastic structure. We further develop its application to address the presence of vertical impermeable faults bounding the reservoir and of multiple injection/extraction sources. Exploiting available information on the reservoir geometry and physical parameters, and records of injection/extraction rates for a gas reservoir in southern Europe, we perform an extensive parametric study considering different model configurations. Comparing modelled spatiotemporal pore-pressure variations with in situ measurements, we show that the inclusion of vertical impermeable faults provides an improvement in reproducing the observations and results in pore-pressure accumulation near the faults and in a variation of the temporal pore-pressure diffusion pattern. To study the relation between gas storage activity and recorded local microseismicity, we applied different seismicity models based on the estimated porepressure distribution. This analysis helps to understand the spatial distribution of seismicity and its temporal modulation. The results show that the observed microseismicity could be partly linked to the storage activity, but the contribution of tectonic background seismicity cannot be excluded.
The administrative language used in imperial and city chanceries illustrates formal language use in the Early Modern period, as most evident in its syntactic complexity. Since administrative language was considered prestigious by the literate people of the time, the syntactic features in question are increasingly found in other text types as well (Lötscher 1995, Schwitalla 2002). The present paper investigates early newspapers published in the seventeenth and eighteenth centuries to evalute their degree of syntactic complexity and hence the extent of formal language used. Contrary to common belief (Admoni 1980, von Polenz 2013), it will be shown that early newspapers do not allow a uniform assessment in terms of their syntactic complexity, when they emerge as a new genre in the seventeenth century: some news segments display a fairly simple syntax, whereas others are of high syntactic complexity. By the end of the eighteenth century, the growing conventionalization of the new genre as well as the impact of standardization processes render newspapers much more balanced in terms of syntactic complexity. Unlike previous work on the syntactic complexity of newspaper language, the measurement of syntactic complexity takes into account not only sentence length and the relationship between independent and dependent clauses, but also the placement of adverbial clauses in relation to their associated clause.
Reservoir-triggered seismicity has been observed near dams during construction, impoundment, and cyclic filling in many parts of the earth. In Turkey, the number of dams has increased substantially over the last decade, with Ataturk Dam being the largest dam in Turkey with a total water capacity of 48.7 billion m(3). After the construction of the dam, the monitoring network has improved. Considering earthquakes above the long-term completeness magnitude of M-C = 3.5, the local seismicity rate has substantially increased after the filling of the reservoir. Recently, two damaging earthquakes of M-w 5.5 and M-w 5.1 occurred in the town of Samsat near the Ataturk Reservoir in 2017 and 2018, respectively. In this study, we analyze the spatio-temporal evolution of seismicity and its source properties in relation to the temporal water-level variations and the stresses resulting from surface loading and pore-pressure diffusion. We find that water-level and seismicity rate are anti-correlated, which is explained by the stabilization effect of the gravitational induced stress imposed by water loading on the local faults. On the other hand, we find that the overall effective stress in the seismogenic zone increased over decades due to pore-pressure diffusion, explaining the enhanced background seismicity during recent years. Additionally, we observe a progressive decrease of the Gutenberg-Richter b-value. Our results indicate that the stressing rate finally focused on the region where the two damaging earthquakes occurred in 2017 and 2018.
Synthesis of organic-inorganic hybrids based on the conjugated polymer P3HT and mesoporous silicon
(2022)
Organic-inorganic hybrids are a class of functional materials that combine favorable properties of their constituents to achieve an overall improved performance for a wide range of applications. This article presents the synthesis route for P3HT-porous silicon hybrids for thermoelectric applications. The conjugated polymer P3HT is incorporated into the porous silicon matrix by means of melt infiltration. Gravimetry, sorption isotherms and energy dispersive X-ray spectroscopy (EDX) mapping indicate that the organic molecules occupy more than 50% of the void space in the inorganic host. We demonstrate that subsequent diffusion-based doping of the confined polymer in a FeCl3 solution increases the electrical conductivity of the hybrid by five orders of magnitude compared to the empty porous silicon host.
The accelerating climatic changes and new infrastructure development across the Arctic require more robust risk and environmental assessment, but thus far there is no consistent record of human impact. We provide a first panarctic satellite-based record of expanding infrastructure and anthropogenic impacts along all permafrost affected coasts (100 km buffer, approximate to 6.2 Mio km(2)), named the Sentinel-1/2 derived Arctic Coastal Human Impact (SACHI) dataset. The completeness and thematic content goes beyond traditional satellite based approaches as well as other publicly accessible data sources. Three classes are considered: linear transport infrastructure (roads and railways), buildings, and other impacted area. C-band synthetic aperture radar and multi-spectral information (2016-2020) is exploited within a machine learning framework (gradient boosting machines and deep learning) and combined for retrieval with 10 m nominal resolution. In total, an area of 1243 km(2) constitutes human-built infrastructure as of 2016-2020. Depending on region, SACHI contains 8%-48% more information (human presence) than in OpenStreetMap. 221 (78%) more settlements are identified than in a recently published dataset for this region. 47% is not covered in a global night-time light dataset from 2016. At least 15% (180 km(2)) correspond to new or increased detectable human impact since 2000 according to a Landsat-based normalized difference vegetation index trend comparison within the analysis extent. Most of the expanded presence occurred in Russia, but also some in Canada and US. 31% and 5% of impacted area associated predominantly with oil/gas and mining industry respectively has appeared after 2000. 55% of the identified human impacted area will be shifting to above 0 C-circle ground temperature at two meter depth by 2050 if current permafrost warming trends continue at the pace of the last two decades, highlighting the critical importance to better understand how much and where Arctic infrastructure may become threatened by permafrost thaw.
This article presents inelastic thermal neutron scattering experiments probing the phonon dispersion in mesoporous silicon with pores 8 nm across. Scattering studies reveal the energy-momentum relation for transverse and longitudinal phonons along the high symmetry directions , and in the Brillouin zone. The dispersion up to phonon energies of 35 meV unambiguously proves that the phonon group velocities in highly-crystalline silicon are not modified by nanostructuring down to sub-10 nanometer length scales. On these length scales, there is apparently no effect of structuring on the elastic moduli of mesoporous silicon. No evidence can be found for phonon-softening in topologically complex, geometrically disordered mesoporous silicon putting it in contrast to silicon nanotubes and nanoribbons.
Instructional videos are widely used to study teachers' professional vision. A new technological development in video research is mobile eye-tracking (MET). It has the potential to provide fine-grained insights into teachers' professional vision in action, but has yet been scarcely employed. We addressed this research gap by using MET video feedback to examine how expert and novice teachers differed in their noticing and weighing of alternative teaching strategies. Expert and novice teachers' lessons were recorded with MET devices. Then, they commented on what they observe while watching their own teaching videos. Using a mixed methods approach, we found that expert and novice teachers did not differ in the number of classroom events they noticed and alternative teaching strategies they mentioned. However, novice teachers were more critical of their own teaching than expert teachers, particularly when they considered alternative teaching strategies. Practical implications for the field of teacher education are discussed.
We revise the conceptual model of calcite varves and present, for the first time, a dual lake monitoring study in two alkaline lakes providing new insights into the seasonal sedimentation processes forming these varves. The study lakes, Tiefer See in NE Germany and Czechowskie in N Poland, have distinct morphology and bathymetry, and therefore, they are ideal to decipher local effects on seasonal deposition. The monitoring setup in both lakes is largely identical and includes instrumental observation of (i) meteorological parameters, (ii) chemical profiling of the lake water column including water sampling, and (iii) sediment trapping at both bi-weekly and monthly intervals. We then compare our monitoring data with varve micro-facies in the sediment record. One main finding is that calcite varves form complex laminae triplets rather than simple couplets as commonly thought. Sedimentation of varve sub-layers in both lakes is largely dependent on the lake mixing dynamics and results from the same seasonality, commencing with diatom blooms in spring turning into a pulse of calcite precipitation in summer and terminating with a re-suspension layer in autumn and winter, composed of calcite patches, plant fragments and benthic diatoms. Despite the common seasonal cycle, the share of each of these depositional phases in the total annual sediment yield is different between the lakes. In Lake Tiefer See calcite sedimentation has the highest yields, whereas in Lake Czechowskie, the so far underestimated re-suspension sub-layer dominates the sediment accumulation. Even in undisturbed varved sediments, re-suspended material becomes integrated in the sediment fabric and makes up an important share of calcite varves. Thus, while the biogeochemical lake cycle defines the varves' autochthonous components and micro-facies, the physical setting plays an important role in determining the varve sub-layers' proportion.
Non-alcoholic fatty liver disease (NAFLD), as a consequence of overnutrition caused by high-calorie diets, results in obesity and disturbed lipid homeostasis leading to hepatic lipid droplet formation. Lipid droplets can impair hepatocellular function; therefore, it is of utmost importance to degrade these cellular structures. This requires the normal function of the autophagic-lysosomal system and the ubiquitin-proteasomal system. We demonstrated in NZO mice, a polygenic model of obesity, which were compared to C57BL/6J (B6) mice, that a high-fat diet leads to obesity and accumulation of lipid droplets in the liver. This was accompanied by a loss of autophagy efficiency whereas the activity of lysosomal proteases and the 20S proteasome remained unaffected. The disturbance of cellular protein homeostasis was further demonstrated by the accumulation of 3-nitrotyrosine and 4-hydroxynonenal modified proteins, which are normally prone to degradation. Therefore, we conclude that fat accumulation in the liver due to a high-fat diet is associated with a failure of autophagy and leads to the disturbance of proteostasis. This might further contribute to lipid droplet stabilization and accumulation.
Large earthquakes can increase the amount of water feeding stream flows, raise groundwater levels, and thus grant plant roots more access to water in water-limited environments. We examine growth and photosynthetic responses of Pine plantations to the Maule M-w 8.8 earthquake in headwater catchments of Chile's Coastal Range. We combine high-resolution wood anatomic (lumen area) and biogeochemical (delta 13C of wood cellulose) proxies of daily to weekly tree growth sampled from trees on floodplains and close to ridge lines. We find that, immediately after the earthquake, at least two out of six tree trees on valley floors had increased lumen area and decreased delta 13C, while trees on hillslopes had a reverse trend. Our results indicate a control of soil water on this response, largely consistent with models that predict how enhanced postseismic vertical soil permeability causes groundwater levels to rise on valley floors, but fall along the ridges. Statistical analysis with boosted regression trees indicates that streamflow discharge gained predictive importance for photosynthetic activity on the ridges, but lost importance on the valley floor after the earthquake. We infer that earthquakes may stimulate ecohydrological conditions favoring tree growth over days to weeks by triggering stomatal opening. The weak and short-lived signals that we identified, however, show that such responses are only valid under water-limited, rather than energy-limited tree, growth. Hence, dendrochronological studies targeted at annual resolution may overlook some earthquake effects on tree vitality.
The use of post-processing heat treatments is often considered a necessary approach to relax high-magnitude residual stresses (RS) formed during the layerwise additive manufacturing laser powder bed fusion (LPBF). In this work, three heat treatment strategies using temperatures of 450 degrees C, 800 degrees C, and 900 degrees C are applied to austenitic stainless steel 316L samples manufactured by LPBF. These temperatures encompass the suggested lower and upper bounds of heat treatment temperatures of conventionally processed 316L. The relaxation of the RS is characterized by neutron diffraction (ND), and the associated changes of the microstructure are analyzed using electron backscattered diffraction (EBSD) and scanning electron microscopy (SEM). The lower bound heat treatment variant of 450 degrees C for 4 hours exhibited high tensile and compressive RS. When applying subsequent heat treatments, we show that stress gradients are still observed after applying 800 degrees C for 1 hour but almost completely vanish when applying 900 degrees C for 1 hour. The observed near complete relaxation of the RS appears to be closely related to the evolution of the characteristic subgrain solidification cellular microstructure.
The vertical flux of marine snow particles significantly reduces atmospheric carbon dioxide concentration. In the mesopelagic zone, a large proportion of the organic carbon carried by sinking particles dissipates thereby escaping long term sequestration. Particle associated prokaryotes are largely responsible for such organic carbon loss. However, links between this important ecosystem flux and ecological processes such as community development of prokaryotes on different particle fractions (sinking vs. non-sinking) are yet virtually unknown. This prevents accurate predictions of mesopelagic organic carbon loss in response to changing ocean dynamics. Using combined measurements of prokaryotic heterotrophic production rates and species richness in the North Atlantic, we reveal that carbon loss rates and associated microbial richness are drastically different with particle fractions. Our results demonstrate a strong negative correlation between prokaryotic carbon losses and species richness. Such a trend may be related to prokaryotes detaching from fast-sinking particles constantly enriching non-sinking associated communities in the mesopelagic zone. Existing global scale data suggest this negative correlation is a widespread feature of mesopelagic microbes.
The Gravity Recovery and Climate Experiment Follow-On (GRACE-FO) mission carries magnetometers that are dedicated to enhance the satellite's navigation. After appropriate calibration and characterisation of artificial magnetic disturbances, these observations are valuable assets to characterise the natural variability of Earth's magnetic field. We describe the data pre-processing, the calibration, and characterisation strategy against a high-precision magnetic field model applied to the GRACE-FO magnetic data. During times of geomagnetic quiet conditions, the mean residual to the magnetic model is around 1 nT with standard deviations below 10 nT. The mean difference to data of ESA's Swarm mission, which is dedicated to monitor the Earth's magnetic field, is mainly within +/- 10 nT during conjunctions. The performance of GRACE-FO magnetic data is further discussed on selected scientific examples. During a magnetic storm event in August 2018, GRACE-FO reveals the local time dependence of the magnetospheric ring current signature, which is in good agreement with results from a network of ground magnetic observations. Also, derived field-aligned currents (FACs) are applied to monitor auroral FACs that compare well in amplitude and statistical behaviour for local time, hemisphere, and solar wind conditions to approved earlier findings from other missions including Swarm. On a case event, it is demonstrated that the dual-satellite constellation of GRACE-FO is most suitable to derive the persistence of auroral FACs with scale lengths of 180 km or longer. Due to a relatively larger noise level compared to dedicated magnetic missions, GRACE-FO is especially suitable for high-amplitude event studies. However, GRACE-FO is also sensitive to ionospheric signatures even below the noise level within statistical approaches. The combination with data of dedicated magnetic field missions and other missions carrying non-dedicated magnetometers greatly enhances related scientific perspectives.
Antarctica is the last pristine environment on Earth, its biota being adapted to the harsh and extreme polar climate. Until now, soil formation and vegetation development in continental Antarctica were considered very slow due to the extreme conditions of this polar desert. Since the austral summer 2002/2003, a long-term monitoring network of the terrestrial ecosystems (soils, vegetation, active layer thickness) has been established at Victoria Land (VL) across a > 500 km latitudinal gradient of coastal sites (73 degrees -77 degrees S). In only one decade large ecosystem changes were detected. Climate was characterized by a significant increase of thawing degree days in northern VL and of autumn air temperature. No extreme climatic events (such as hot spells) where detected in the study period. Soil chemistry suffered large quantitative changes, clearly indicating rapid pedogenetic processes. In most soils the upper layers exhibited a strong alkalinization (pH increases up to 3 units) and increases in conductivity, anions and cations (in particular of SO4 and Na). The largest changes were observed in soils with low vegetation cover. Statistically significant differences in soil chemistry were detected between soils with high and low vegetation cover, the former showing lower pH, conductivity, Na and Cl. Most plots exhibited changes of total cover, species richness and floristic composition, with vegetation expansion in soils with low vegetation cover and the largest increase recorded at Apostrophe Island (northern VL). Principal Component Analysis (PCA) identified the main trend of vegetation change, with a shift from lower to higher cover and a secondary trend of change associated with a gradient of water availability, consistent with an increase in water instead of snow. Redundancy analysis (RDA) identified the trend of change in soil chemistry with increases in pH, conductivity, anions and cations associated with the concomitant decrease in C, N, NO3, PO4. The RDA confirmed that soil changes were associated with a gradient of vegetation change (from low to high cover) as well as of water availability, as already indirectly outlined by the PCA. Field manipulation experiments carried out at five locations of the network between 73 degrees S and 77 degrees S, simulating increases of precipitation from snow or water additions didn't induce changes in soil pH, indicating that pulse events of snow accumulation or melting could not trigger persistent soil pH changes. These data allow hypothesize the occurrence of a main ecosystem change occurring at regional scale at Victoria Land. The slight air warming and its consequences on soil chemistry and vegetation, further highlight the sensitivity of the fragile Antarctic ecosystems to the consequences of even small changes in climate.
Seafloor spreading at slow rates can be accommodated on large-offset oceanic detachment faults (ODFs), that exhume lower crustal and mantle rocks in footwall domes termed oceanic core complexes (OCCs). Footwall rocks experience large rotation during exhumation, yet important aspects of the kinematics-particularly the relative roles of solid-block rotation and flexure-are not clearly understood. Using a high-resolution numerical model, we explore the exhumation kinematics in the footwall beneath an emergent ODF/OCC. A key feature of the models is that footwall motion is dominated by solid-block rotation, accommodated by the nonplanar, concave-down fault interface. A consequence is that curvature measured along the ODF is representative of a neutral stress configuration, rather than a "bent" one. Instead, it is in the subsequent process of "apparent unbending" that significant flexural stresses are developed in the model footwall. The brittle strain associated with apparent unbending is produced dominantly in extension, beneath the OCC, consistent with earthquake clustering observed in the Trans-Atlantic Geotraverse at the Mid-Atlantic Ridge.
We consider sedimented at a solid wall particles that are immersed in water containing small additives of photosensitive ionic surfactants. It is shown that illumination with an appropriate wavelength, a beam intensity profile, shape and size could lead to a variety of dynamic, both unsteady and steady state, configurations of particles. These dynamic, well-controlled and switchable particle patterns at the wall are due to an emerging diffusio-osmotic flow that takes its origin in the adjacent to the wall electrostatic diffuse layer, where the concentration gradients of surfactant are induced by light. The conventional nonporous particles are passive and can move only with already generated flow. However, porous colloids actively participate themselves in the flow generation mechanism at the wall, which also sets their interactions that can be very long ranged. This light-induced diffusio-osmosis opens novel avenues to manipulate colloidal particles and assemble them to various patterns. We show in particular how to create and split optically the confined regions of particles of tunable size and shape, where well-controlled flow-induced forces on the colloids could result in their crystalline packing, formation of dilute lattices of well-separated particles, and other states.
Mental health of Japanese workers: amotivation mediates self-compassion on mental health problems
(2022)
Workplace mental health is a cause for concern in many countries. Globally, 78% of the workforce experienced impairment of their mental health in 2020. In Japan, more than half of employees are mentally distressed.
Previously, research has identified that self-compassion (i.e., being kind and understanding towards oneself) and work motivation were important to their mental health.
However, how these three components relate to each other remains to be elucidated. Accordingly, this study aimed to examine the relationship between mental health problems, self-compassion and work motivation (i.e., intrinsic motivation, extrinsic motivation and amotivation).
A cross-sectional design was employed, where 165 Japanese workers completed self-report scales regarding those three components. A correlation and path analyses were conducted.
Mental health problems were positively associated with amotivation and negatively associated with age and self-compassion. While intrinsic motivation and extrinsic motivation did not mediate the impact of self-compassion on mental health problems, amotivation did.
The findings can help managers and organizational psychologists help identify effective approaches to improving work mental health.
Fungal biotransformation is an attractive synthetic strategy to produce highly specific compounds with chemical functionality in regions of the carbon skeleton that are not easily activated by conventional organic chemistry methods.
In this work, Cladosporium antarcticum isolated from sediments of Glacier Collins in Antarctica was used to obtain novel drimane sesquiterpenoids alcohols with activity against Candida yeast from drimendiol and epidrimendiol. These compounds were produced by the high-yield reduction of polygodial and isotadeonal with NaBH4 in methanol.
Cladosporium antarcticum produced two major products from drimendiol, identified as 9 alpha-hydroxydrimendiol (1, 41.4 mg, 19.4% yield) and 3 beta-hydroxydrimendiol (2, 74.8 mg, 35% yield), whereas the biotransformation of epidrimendiol yielded only one product, 9 beta-hydroxyepidrimendiol (3, 86.6 mg, 41.6% yield).
The products were purified by column chromatography and their structure elucidated by NMR and MS. The antifungal activity of compounds 1-3 was analyzed against Candida albicans, C. krusei and C. parapsilosis, showing that compound 2 has a MIC lower than 15 mu g/mL against the three-pathogenic yeast.
In silico studies suggest that a possible mechanism of action for the novel compounds is the inhibition of the enzyme lanosterol 14 alpha-demethylase, affecting the ergosterol synthesis.
The wetland cover is defined as the spatially homogenous region of a wetland attributed to the underlying biophysical conditions such as vegetation, turbidity, hydric soil, and the amount of water.
Here, we present a novel method to derive the wetland-cover types (WCTs) combining three commonly used multispectral indices, NDVI, MNDWI, and NDTI, in three large Ramsar wetlands located in different geomorphic and climatic settings across India. These wetlands include the Kaabar Tal, a floodplain wetland in east Ganga Plains, Chilika Lagoon, a coastal wetland in eastern India, and Nal Sarovar in semi-arid western India.
The novelty of our approach is that the derived WCTs are stable in space and time, and therefore, a given WCT across different wetlands or within different zones of a large wetland will imply similar underlying biophysical attributes.
The WCTs can therefore provide a novel tool for monitoring and change detection of wetland cover types.
We have automated the proposed WCT algorithm using the Google Earth Engine (GEE) environment and by developing ArcGIS tools. The method can be implemented on any wetland and using any multispectral imagery dataset with visible and NIR bands.
The proposed methodology is simple yet robust and easy to implement and, therefore, holds significant importance in wetland monitoring and management.
Introduction:
Positively conditioned Pavlovian cues tend to promote approach and negative cues promote withdrawal in a Pavlovian-to-instrumental transfer (PIT) paradigm, and the strength of this PIT effect was associated with the subsequent relapse risk in alcohol-dependent (AD) patients.
When investigating the effect of alcohol-related background cues, instrumental approach behavior was inhibited in subsequent abstainers but not relapsers. An automatic approach bias towards alcohol can be modified using a cognitive bias modification (CBM) intervention, which has previously been shown to reduce the relapse risk in AD patients. Here we examined the effects of such CBM training on PIT effects and explored its effect on the relapse risk in detoxified AD patients.
Methods:
N = 81 recently detoxified AD patients performed non-drug-related and drug-related PIT tasks before and after CBM versus placebo training. In addition, an alcohol approach/avoidance task (aAAT) was performed before and after the training to assess the alcohol approach bias. Patients were followed up for 6 months.
Results:
A stronger alcohol approach bias as well as a stronger non-drug-related PIT effect predicted relapse status in AD patients. No significant difference regarding relapse status or the number of heavy drinking days was found when comparing the CBM training group versus the placebo group.
Moreover, there was no significant modulation effect of CBM training on any PIT effect or the aAAT.
Conclusion:
A higher alcohol approach bias in the aAAT and a stronger non-drug-related PIT effect both predicted relapse in AD patients, while treatment outcome was not associated with the drug-related PIT effect. Unlike expected, CBM training did not significantly interact with the non-drug-related or the drug-related PIT effects or the alcohol approach bias.
Materials realizing the XY model in two dimensions are sparse.
Here we use neutron triple-axis spectroscopy to investigate the critical static and dynamical magnetic fluctuations in the square-lattice antiferromagnets Ca2RuO4 and Ca3Ru2O7.
We probe the temperature dependence of the antiferromagnetic Bragg intensity, the Q width, the amplitude, and the energy width of the magnetic diffuse scattering in the vicinity of the Neel temperature T-N to determine the critical behavior of the magnetic order parameter M, correlation length xi, susceptibility chi, and the characteristic energy Gamma with the corresponding critical exponents beta, nu, gamma, and z, respectively.
We find that the critical behaviors of the single-layer compound Ca2RuO4 follow universal scaling laws that are compatible with predictions of the two-dimensional (2D) XY model.
The bilayer compound Ca3Ru2O7 is only partly consistent with the 2D XY theory and best described by the three-dimensional (3D) Ising model, which is likely a consequence of the intrabilayer exchange interactions in combination with an orthorhombic single-ion anisotropy.
Hence, our results suggest that layered ruthenates are promising solid-state platforms for research on the 2D XY model and the effects of 3D interactions and additional spin-space anisotropies on the magnetic fluctuations.
Mitochondrial stress-induced GFRAL signaling controls diurnal food intake and anxiety-like behavior
(2022)
Growth differentiation factor 15 (GDF15) is a mitochondrial stressinduced cytokine that modulates energy balance in an endocrine manner.
However, the importance of its brainstem-restricted receptor GDNF family receptor alpha-like (GFRAL) to mediate endocrine GDF15 signaling to the brain uponmitochondrial dysfunction is still unknown. Using a mouse model with muscle-specific mitochondrial dysfunction, we here show that GFRAL is required for activation of systemic energy metabolism via daytime-restricted anorexia but not responsible for muscle wasting.
We further find that muscle mitochondrial stress response involves a GFRAL-dependent induction of hypothalamic corticotropin-releasing hormone, without elevated corticosterone levels.
Finally, we identify that GFRAL signaling governs an anxiety-like behavior in male mice with muscle mitochondrial dysfunction, with females showing a less robust GFRAL-dependent anxiety-like phenotype.
Together, we here provide novel evidence of a mitochondrial stress-induced muscle-brain crosstalk via the GDF15-GFRAL axis to modulate food intake and anxiogenic behavior.
In star-forming galaxies, the far-infrared (FIR) and radio-continuum luminosities obey a tight empirical relation over a large range of star-formation rates (SFR).
To understand the physics, we examine magnetohydrodynamic galaxy simulations, which follow the genesis of cosmic ray (CR) protons at supernovae and their advective and anisotropic diffusive transport.
We show that gravitational collapse of the proto-galaxy generates a corrugated accretion shock, which injects turbulence and drives a small-scale magnetic dynamo. As the shock propagates outwards and the associated turbulence decays, the large velocity shear between the supersonically rotating cool disc with respect to the (partially) pressure-supported hot circumgalactic medium excites Kelvin-Helmholtz surface and body modes.
Those interact non-linearly, inject additional turbulence and continuously drive multiple small-scale dynamos, which exponentially amplify weak seed magnetic fields.
After saturation at small scales, they grow in scale to reach equipartition with thermal and CR energies in Milky Way-mass galaxies. In small galaxies, the magnetic energy saturates at the turbulent energy while it fails to reach equipartition with thermal and CR energies.
We solve for steady-state spectra of CR protons, secondary electrons/positrons from hadronic CR-proton interactions with the interstellar medium, and primary shock-accelerated electrons at supernovae.
The radio-synchrotron emission is dominated by primary electrons, irradiates the magnetized disc and bulge of our simulated Milky Way-mass galaxy and weakly traces bubble-shaped magnetically loaded outflows.
Our star-forming and star-bursting galaxies with saturated magnetic fields match the global FIR-radio correlation (FRC) across four orders of magnitude. Its intrinsic scatter arises due to (i) different magnetic saturation levels that result from different seed magnetic fields, (ii) different radio synchrotron luminosities for different specific SFRs at fixed SFR, and (iii) a varying radio intensity with galactic inclination.
In agreement with observations, several 100-pc-sized regions within star-forming galaxies also obey the FRC, while the centres of starbursts substantially exceed the FRC.
A key in controlling the SARS-CoV-2 pandemic is the assessment of the immune status of the population. We explored the utility of SARS-CoV-2 virus-like particles (VLPs) as antigens to detect specific humoral immune reactions in an enzyme-linked immunosorbent assay (ELISA).
For this purpose, SARS-CoV-2 VLPs were produced from an engineered cell line and characterized by Western blot, ELISA, and nanoparticle tracking analysis.
Subsequently, we collected 42 serum samples from before the pandemic (2014), 89 samples from healthy subjects, and 38 samples from vaccinated subjects. Seventeen samples were collected less than three weeks after infection, and forty-four samples more than three weeks after infection.
All serum samples were characterized for their reactivity with VLPs and the SARS-CoV-2 N- and S-protein.
Finally, we compared the performance of the VLP-based ELISA with a certified in vitro diagnostic device (IVD). In the applied set of samples, we determined a sensitivity of 95.5% and a specificity of 100% for the certified IVD.
There were seven samples with an uncertain outcome. Our VLP-ELISA demonstrated a superior performance, with a sensitivity of 97.5%, a specificity of 100%, and only three uncertain outcomes.
This result warrants further research to develop a certified IVD based on SARS-CoV-2 VLPs as an antigen.
Adults' ratings of children's personality have been found to be more closely associated with academic performance than children's self-reports. However, less is known about the relevance of the unique perspectives held by specific adult observers such as teachers and parents for explaining variance in academic performance. In this study, we applied bifactor (S-1) models for 1411 elementary school children to investigate the relative merits of teacher and parent ratings of children's personalities for academic performance above and beyond the children's self-reports. We examined these associations using standardized achievement test scores in addition to grades. We found that teachers' unique views on children's openness and conscientiousness had the strongest associations with academic performance. Parents' unique views on children's neuroticism showed incremental associations above teacher ratings or self-reports. For extraversion and agreeableness, however, children's self-reports were more strongly associated with academic performance than teacher or parent ratings. These results highlight the differential value of using multiple informants when explaining academic performance with personality traits.
Rising childhood obesity with its detrimental health consequences poses a challenge to the health care system. Community-based, multi-setting interventions with the participatory involvement of relevant stakeholders are emerging as promising. To gain insights into the structural and processual characteristics of stakeholder networks, conducting a network analysis (NA) is advisable. Within the program "Family+-Healthy Living Together in Families and Schools", a network analysis was conducted in two rural model regions and one urban model region. Relevant stakeholders were identified in 2020-2021 through expert interviews and interviewed by telephone to elicit key variables such as frequency of contact and intensity of collaboration. Throughout the NA, characteristics such as density, centrality, and connectedness were analyzed and are presented graphically. Due to the differences in the number of inhabitants and the rural or urban structure of the model regions, the three networks (network#1, network#2, and network#3) included 20, 14, and 12 stakeholders, respectively. All networks had similar densities (network#1, 48%; network#2, 52%; network#3, 42%), whereas the degree centrality of network#1 (0.57) and network#3 (0.58) was one-third higher compared with network#2 (0.39). All three networks differed in the distribution of stakeholders in terms of field of expertise and structural orientation. On average, stakeholders exchanged information quarterly and were connected on an informal level. Based on the results of the NA, it appears to be useful to initialize a community health facilitator to involve relevant stakeholders from the education, sports, and health systems in projects and to strive for the goal of sustainable health promotion, regardless of the rural or urban structure of the region. Participatory involvement of relevant stakeholders can have a positive influence on the effective dissemination of information and networking with other stakeholders.
The Alpine mountains in central Europe are characterized by a heterogeneous crust accumulating different tectonic units and blocks in close proximity to sedimentary foreland basins. Centroid moment tensor inversion provides insight into the faulting mechanisms of earthquakes and related tectonic processes but is significantly aggravated in such an environment. Thanks to the dense AlpArray seismic network and our flexible bootstrap-based inversion tool Grond, we are able to test different setups with respect to the uncertainties of the obtained moment tensors and centroid locations. We evaluate the influence of frequency bands, azimuthal gaps, input data types, and distance ranges and study the occurrence and reliability of non-double-couple (DC) components. We infer that for most earthquakes (M-w >= 3.3) a combination of time domain full waveforms and frequency domain amplitude spectra in a frequency band of 0.02-0.07 Hz is suitable. Relying on the results of our methodological tests, we perform deviatoric moment tensor (MT) inversions for events with M-w > 3.0. Here, we present 75 solutions for earthquakes between January 2016 and December 2019 and analyze our results in the seismotectonic context of historical earthquakes, seismic activity of the last 3 decades, and GNSS deformation data. We study regions of comparably high seismic activity during the last decades, namely the Western Alps, the region around Lake Garda, and the eastern Southern Alps, as well as clusters further from the study region, i.e., in the northern Dinarides and the Apennines. Seismicity is particularly low in the Eastern Alps and in parts of the Central Alps. We apply a clustering algorithm to focal mechanisms, considering additional mechanisms from existing catalogs. Related to the N-S compressional regime, E-W-to-ENE-WSW-striking thrust faulting is mainly observed in the Friuli area in the eastern Southern Alps. Strike-slip faulting with a similarly oriented pressure axis is observed along the northern margin of the Central Alps and in the northern Dinarides. NW-SE-striking normal faulting is observed in the NW Alps, showing a similar strike direction to normal faulting earthquakes in the Apennines. Both our centroid depths and hypocentral depths in existing catalogs indicate that Alpine seismicity is predominantly very shallow; about 80% of the studied events have depths shallower than 10 km.
The magnitude of earthquakes on continental normal faults rarely exceeds 7.0 Mw. However, because of their vicinity to large population centers they can be highly destructive.
Long recurrence time, relatively small deformations, and limited observations hinder our understanding of the deformation patterns and mechanisms controlling the magnitude of events.
Here, this problem is addressed with 2D thermomechanical modeling of normal fault seismic cycles.
The 2020 Samos, Greece Mw7.0 earthquake is used as an example as it is one of the largest and most studied continental normal fault earthquakes. The modeling approach employs visco-elasto-plastic rheology, compressibility, free surface, and a rate-and-state friction law for the fault.
Modeling of the Samos earthquake suggests the pore fluid pressure ratio on the fault ranges from 0 to 0.7. The model demonstrates that most of the deformation during interseismic and coseismic periods, besides on the fault, occurs in the hanging wall and footwall below the seismogenic part of the fault. The largest vertical surface displacement during the earthquake is the subsidence of the hanging wall in the vicinity of the fault, while the uplift of the footwall and remote part of the hanging wall is significantly smaller.
Modeling of the seismic cycles on normal faults with different setups shows the dependency of the magnitude on the thermal profile and dipping angle of the fault; low heat flow and low dipping angle are favorable conditions for the largest events, while steep normal faults in the areas of high heat flow tend to have the smallest magnitudes.
In a warming Arctic, permafrost-related disturbances, such as retrogressive thaw slumps (RTS), are becoming more abundant and dynamic, with serious implications for permafrost stability and bio-geochemical cycles on local to regional scales. Despite recent advances in the field of earth observation, many of these have remained undetected as RTS are highly dynamic, small, and scattered across the remote permafrost region. Here, we assessed the potential strengths and limitations of using deep learning for the automatic segmentation of RTS using PlanetScope satellite imagery, ArcticDEM and auxiliary datasets. We analyzed the transferability and potential for pan-Arctic upscaling and regional cross-validation, with independent training and validation regions, in six different thaw slump-affected regions in Canada and Russia. We further tested state-of-the-art model architectures (UNet, UNet++, DeepLabv3) and encoder networks to find optimal model configurations for potential upscaling to continental scales. The best deep learning models achieved mixed results from good to very good agreement in four of the six regions (maxIoU: 0.39 to 0.58; Lena River, Horton Delta, Herschel Island, Kolguev Island), while they failed in two regions (Banks Island, Tuktoyaktuk). Of the tested architectures, UNet++ performed the best. The large variance in regional performance highlights the requirement for a sufficient quantity, quality and spatial variability in the training data used for segmenting RTS across diverse permafrost landscapes, in varying environmental conditions. With our highly automated and configurable workflow, we see great potential for the transfer to active RTS clusters (e.g., Peel Plateau) and upscaling to much larger regions.
Background Mass gatherings (MGs) such as music festivals and sports events have been associated with a high risk of SARS-CoV-2 transmission. On-site research can foster knowledge of risk factors for infections and improve risk assessments and precautionary measures at future MGs. We tested a web-based participatory disease surveillance tool to detect COVID-19 infections at and after an outdoor MG by collecting self-reported COVID-19 symptoms and tests. Methods We conducted a digital prospective observational cohort study among fully immunized attendees of a sports festival that took place from September 2 to 5, 2021 in Saxony-Anhalt, Germany. Participants used our study app to report demographic data, COVID-19 tests, symptoms, and their contact behavior. This self-reported data was used to define probable and confirmed COVID-19 cases for the full "study period" (08/12/2021 - 10/31/2021) and within the 14-day "surveillance period" during and after the MG, with the highest likelihood of an MG-related COVID-19 outbreak (09/04/2021 - 09/17/2021). Results A total of 2,808 of 9,242 (30.4%) event attendees participated in the study. Within the study period, 776 individual symptoms and 5,255 COVID-19 tests were reported. During the 14-day surveillance period around and after the MG, seven probable and seven PCR-confirmed COVID-19 cases were detected. The confirmed cases translated to an estimated seven-day incidence of 125 per 100,000 participants (95% CI [67.7/100,000, 223/100,000]), which was comparable to the average age-matched incidence in Germany during this time. Overall, weekly numbers of COVID-19 cases were fluctuating over the study period, with another increase at the end of the study period. Conclusion COVID-19 cases attributable to the mass gathering were comparable to the Germany-wide age-matched incidence, implicating that our active participatory disease surveillance tool was able to detect MG-related infections. Further studies are needed to evaluate and apply our participatory disease surveillance tool in other mass gathering settings.
The formation of the Central Andes dates back to similar to 50 Ma, but its most pronounced episode, including the growth of the Altiplano-Puna Plateau and pulsatile tectonic shortening phases, occurred within the last 25 Ma.
The reason for this evolution remains unexplained. Using geodynamic numerical modeling we infer that the primary cause of the pulses of tectonic shortening and growth of the Central Andes is the changing geometry of the subducted Nazca plate, and particularly the steepening of the mid-mantle slab segment which results in a slowing down of the trench retreat and subsequent increase in shortening of the advancing South America plate.
This steepening first happens after the end of the flat slab episode at similar to 25 Ma, and later during the buckling and stagnation of the slab in the mantle transition zone. Processes that mechanically weaken the lithosphere of the South America plate, as suggested in previous studies, enhance the intensity of the shortening events.
These processes include delamination of the mantle lithosphere and weakening of foreland sediments.
Our new modeling results are consistent with the timing and amplitude of the deformation from geological data in the Central Andes at the Altiplano latitude.
Plain Language Summary
The Central Andes is a subduction-type orogeny that formed as a result of the interaction between the Nazca oceanic plate and the South American continental plate over the last 50 million years. Growth of the Andes is primarily the result of crustal shortening. Nevertheless, "geological" data compiled from previous studies have shown that phases of drastic pulsatile shortening occur at 15 and 5 Ma.
In this study, we used high-resolution 2D numerical geodynamic simulations to investigate the link between oceanic and continental plate dynamics and their interaction. We find that when the oceanic plate steepens in the mantle transition zone, the trench retreat is hindered. Coupled with the weakening of the continental plate through the slab flattening and subsequent delamination of the lithospheric mantle, this leads to pulsatile shortening phases of a magnitude equivalent to that suggested by the data.
Magma-filled dikes may feed erupting fissures that lead to alignments of craters developing at the surface, yet the details of activity and migrating eruptions at the crater row are difficult to monitor and are hardly understood.
The 2021 Tajogaite eruption at the Cumbre Vieja, La Palma (Spain), lasted 85 days and developed a pronounced alignment of craters that may be related to changes within the volcano edifice.
Here, we use COSMO-SkyMed satellite radar data and ground-based time-lapse photographs, offering a high-resolution dataset to explore the locations and characteristics of evolving craters.
Our results show that the craters evolve both gradually and suddenly and can be divided into three main phases. Phase 1, lasting the first 6 weeks of the eruption, was characterized by a NW-SE linear evolution of up to seven craters emerging on the growing cone.
Following two partial collapses of the cone to the northwest and a seismicity increase at depth, Phase 2 started and caused a propagation of the main activity toward the southeastern side, together with the presence of up to 11 craters along this main NW-SE trend. Associated with strong deep and shallow earthquakes, Phase 3 was initiated and continued for the final 2 weeks of the eruption, expressed by the development of up to 18 craters, which became dominant and clustered in the southeastern sector in early December 2021. In Phase 3, a second and oblique alignment and surface fracture was identified.
Our findings that crater and eruption changes coincide together with an increase in seismic activity at depth point to a deep driver leading to crater and morphology changes at the surface.
These also suggest that crater distributions might allow for improved monitoring of changes occurring at depth, and vice versa, such that strong seismicity changes at depth may herald the migration and new formation of craters, which have major implications for the assessment of tephra and lava flow hazards on volcanoes.
Drimane sesquiterpene aldehydes control Candida yeast isolated from candidemia in Chilean patients
(2022)
Drimys winteri J.R. (Winteraceae) produce drimane sesquiterpenoids with activity against Candida yeast.
In this work, drimenol, polygodial (1), isotadeonal (2), and a new drimane alpha,beta-unsaturated 1,4-dialdehyde, named winterdial (4), were purified from barks of D. winteri. The oxidation of drimenol produced the monoaldehyde drimenal (3).
These four aldehyde sesquiterpenoids were evaluated against six Candida species isolated from candidemia patients in Chilean hospitals.
Results showed that 1 displays fungistatic activity against all yeasts (3.75 to 15.0 mu g/mL), but irritant effects on eyes and skin, whereas its non-pungent epimer 2 has fungistatic and fungicide activities at 1.9 and 15.0 mu g/mL, respectively.
On the other hand, compounds 3 and 4 were less active.
Molecular dynamics simulations suggested that compounds 1-4 are capable of binding to the catalytic pocket of lanosterol 14-alpha demethylase with similar binding free energies, thus suggesting a potential mechanism of action through the inhibition of ergosterol synthesis. According to our findings, compound 2 appears as a valuable molecular scaffold to pursue the future development of more potent drugs against candidiasis with fewer side effects than polygodial.
These outcomes are significant to broaden the alternatives to treat fungal infections with increasing prevalence worldwide using natural compounds as a primary source for active compounds.
Deriving soil moisture content (SMC) at the regional scale with different spatial and temporal land cover changes is still a challenge for active and passive remote sensing systems, often coped with machine learning methods.
So far, the reference measurements of the data-driven approaches are usually based on point data, which entails a scale gap to the resolution of the remote sensing data. Cosmic Ray Neutron Sensing (CRNS) indirectly provides SMC estimates of a soil volume covering more than 1 ha and vertical depth up to 80 cm and is thus able to narrow this scale gap.
So far, the CRNS-based SMC has only been used as validation source of remote sensing based SMC products. Its beneficial large sensing volume, especially in depth, has not been exploited yet.
However, the sensing volume of the CRNS, which is changing with hydrological conditions, bears challenges for the comparison with remote sensing observations. This study, for the fist time, aims to understand the direct linkage of optical (Sentinel 2) and SAR (Sentinel 1) data with CRNS-based SMC.
Thereby, the CRNS-based SMC is obtained by an experimental CRNS cluster that covers the high temporal and spatial SMC variability of an entire pre-alpine subcatchment. Using different Random Forest regressions, we analyze the potentials and limitations of both remote sensing sensors to follow the CRNS-based SMC signal.
Our results show that it is possible to link the CRNS-based SMC signal with SAR and optical remote sensing observations via Random Forest modelling.
We found that Sentinel 2 data is able to separate wet from dry periods with a R2 of 0.68.
It is less affected by the changing soil volume that contributes to the CRNS-based SMC signal and it is able to assign a land cover specific SMC distribution.
However, Sentinel 2 regression models are not accurate (R2 < 0.21) in mapping the CRNSbased SMC for the frequently mowed grassland areas of the study site. It requires soil type and topographical information to accurately follow the CRNS-based SMC signal with Random Forest regression.
Sentinel 1 data instead is affected by the changing soil volume that contributes to the CRNS-based SMC signal. It has reasonable model performance (R2 = 0.34) when the CRNS data correspond to surface SMC. Also for Sentinel 1 the retrieval is impacted by the mowing activities at the test site.
When separating the CRNS data set into dry and wet periods, soil properties and topography are the main drivers of SMC estimation. Sentinel 1 or Sentinel 2 data add the existing temporal variability to the regression models. The analysis underlines the need of combining optical and SAR observations (Sentinel 1, Sentinel 2) as well as soil property and topographical information to understand and follow the CRNS-based SMC signal for different hydrological conditions and land cover types.
This article explores the evolution of Cultural Linguistics, its fusion with Cognitive Linguistics and Cognitive Sociolinguistics, and its application to the study of world Englishes, emphasising the cultural dimension of language and cognition. It investigates key theoretical concepts in Cultural Linguistics such as cultural categories, schemas, conceptualisations, keywords, models and scenarios as essential analytical tools for examining the interplay between thought, language and culture. Using examples from English varieties in sub-Saharan Africa, Great Britain, Ireland, India and Hong Kong, this article demonstrates how these conceptual phenomena interact at increasing levels of conceptual complexity. The discussion also distinguishes conceptual metaphor (and metonymy) from the somewhat problematic concept of ‘cultural metaphor’, previously used in some cultural-linguistic approaches to world Englishes. Finally, the article delves into Conceptual Blending Theory as a possible extension of Cultural Linguistics that synthesises diverse cultural knowledge to interpret culture-specific expressions in contemporary multilingual settings.
Background
In cystic fibrosis (CF), acute respiratory exacerbations critically enhance pulmonary destruction. Since these mainly occur outside regular appointments, they remain unexplored. We previously elaborated a protocol for home-based upper airway (UAW) sampling obtaining nasal-lavage fluid (NLF), which, in contrast to sputum, does not require immediate processing. The aim of this study was to compare UAW inflammation and pathogen colonization during stable phases and exacerbations in CF patients and healthy controls.
Methods
Initially, we obtained NLF by rinsing 10 ml of isotonic saline/nostril during stable phases. During exacerbations, subjects regularly collected NLF at home. CF patients directly submitted one aliquot for microbiological cultures. The remaining samples were immediately frozen until transfer on ice to our clinic, where PCR analyses were performed and interleukin (IL)-1 beta/IL-6/IL-8, neutrophil elastase (NE), matrix metalloproteinase (MMP)-9, and tissue inhibitor of metalloproteinase (TIMP)-1 were assessed.
Results
Altogether, 49 CF patients and 38 healthy controls (HCs) completed the study, and 214 NLF samples were analyzed. Of the 49 CF patients, 20 were at least intermittently colonized with P. aeruginosa and received azithromycin and/or inhaled antibiotics as standard therapy. At baseline, IL-6 and IL-8 tended to be elevated in CF compared to controls. During infection, inflammatory mediators increased in both cohorts, reaching significance only for IL-6 in controls (p=0.047). Inflammatory responses tended to be higher in controls [1.6-fold (NE) to 4.4-fold (MMP-9)], while in CF, mediators increased only moderately [1.2-1.5-fold (IL-6/IL-8/NE/TIMP-1/MMP-9)]. Patients receiving inhalative antibiotics or azithromycin (n=20 and n=15, respectively) revealed lower levels of IL-1 beta/IL-6/IL-8 and NE during exacerbation compared to CF patients not receiving those antibiotics. In addition, CF patients receiving azithromycin showed MMP-9 levels significantly lower than CF patients not receiving azithromycin at stable phase and exacerbation. Altogether, rhinoviruses were the most frequently detected virus, detected at least once in n=24 (49.0%) of the 49 included pwCF and in n=26 (68.4%) of the 38 healthy controls over the 13-month duration of the study. Remarkably, during exacerbation, rhinovirus detection rates were significantly higher in the HC group compared to those in CF patients (65.8% vs. 22.4%; p<0.0001).
Conclusion
Non-invasive and partially home-based UAW sampling opens new windows for the assessment of inflammation and pathogen colonization in the unified airway system.
This research bibliography lists some of the hallmark works in the field of Cultural Linguistics and has an exclusive thematic focus on cultural-linguistic approaches to world Englishes. Therefore, other important and congenial works that have been published under the umbrella of, for example, Conceptual Metaphor Theory (CMT) or deal with langauges other than English are excluded but can be found in the respective references of the individual contributions of this special issue. The research bibliography offers reference works for research strands of the world Englishes framework, such as English language teaching and language use in multicultural and multilingual contexts, as well as language use in the public space. Furthermore, with a collection of publications ranging from the 1980s to most recent state-of-the-art works from the year 2024, the authors identify trends and topical developments in the synthesized research of Cultural Linguistics and world Englishes and offer an outlook on new frontiers in this realm.
The Sea of Marmara is a tectonically active basin that straddles the North Anatolian Fault Zone (NAFZ), a major strike-slip fault that separates the Eurasian and Anatolian tectonic plates. The Main Marmara Fault (MMF), which is part of the NAFZ, contains an approximately 150 km long seismotectonic segment that has not ruptured since 1766. A key question for seismic hazard and risk assessment is whether or not the next rupture along this segment is likely to produce one major earthquake or a series of smaller earthquakes. Geomechanical characteristics such as along-strike variations in rock strength may provide an important control on seismotectonic segmentation. We find that variations in lithospheric strength throughout the Marmara region control the mechanical segmentation of the MMF and help explain its long-term seismotectonic segmentation. In particular, a strong crust that is mechanically coupled to the upper mantle spatially correlates with aseismic patches, where the MMF bends and changes its strike in response to the presence of high-density lower crustal bodies. Between the bends, mechanically weaker crustal domains that are decoupled from the mantle indicate a predominance of creeping. These results are highly relevant for the ongoing debate regarding the characteristics of the Marmara seismic gap, especially in view of the seismic hazard (Mw > 7) in the densely populated Marmara region.
Simple Summary
Urokinase-type plasminogen activator (urokinase, uPA) is a widely discussed biomarker for cancer prognosis and diagnosis. The gold standard for the determination of protein biomarkers in physiological samples is the enzyme-linked immunosorbent assay (ELISA). Here, antibodies are used to detect the specific protein.
In our study, recently published urokinase aptamers were tested for their use in a sandwich assay format as alternative specific recognition elements. Different aptamer combinations were used for the detection of uPA in a sandwich-assay format and a combination of aptamers and antibodies additionally allowed the differentiation of human high and low molecular weight- (HMW- and LMW-) uPA. Hence, uPA aptamers offer a valuable alternative as specific recognition elements for analytical purposes. Since aptamers are easy to synthesize and modify, they can be used as a cost-effective alternative in sandwich assay formats for the detection of uPA in physiological samples.
Abstract
Urokinase-type plasminogen activator (urokinase, uPA) is a frequently discussed biomarker for prognosis, diagnosis, and recurrence of cancer.
In a previous study, we developed ssDNA aptamers that bind to different forms of human urokinase, which are therefore assumed to have different binding regions.
In this study, we demonstrate the development of aptamer-based sandwich assays that use different combinations of these aptamers to detect high molecular weight- (HMW-) uPA in a micro titer plate format.
By combining aptamers and antibodies, it was possible to distinguish between HMW-uPA and low molecular weight- (LMW-) uPA.
For the best performing aptamer combination, we calculated the limit of detection (LOD) and limit of quantification (LOQ) in spiked buffer and urine samples with an LOD up to 50 ng/mL and 138 ng/mL, respectively.
To show the specificity and sequence dependence of the reporter aptamer uPAapt-02-FR, we have identified key nucleotides within the sequence that are important for specific folding and binding to uPA using a fluorescent dye-linked aptamer assay (FLAA). Since uPA is a much-discussed marker for prognosis and diagnosis in various types of cancers, these aptamers and their use in a micro titer plate assay format represent a novel, promising tool for the detection of uPA and for possible diagnostic applications.
We review properties and processes of earthquake rupture zones based on field studies, laboratory observations, theoretical models and simulations, with the goal of assessing the possible dominance of different processes in different parts of the rupture and validity of commonly used models. Rupture zones may be divided into front, intermediate, and tail regions that interact to different extents. The rupture front is dominated by fracturing and granulation processes and strong dilatation, producing faulting products that are reworked by subsequent sliding behind. The intermediate region sustains primarily frictional sliding with relatively high slip rates that produce appreciable stress transfer to the propagating front. The tail region further behind is characterized by low slip rates that effectively do not influence the propagating front, although it (and the intermediate region) can spawn small offspring rupture fronts. Wave-mediated stress transfer can also trigger failures ahead of the rupture front. Earthquake ruptures are often spatially discontinuous and intermittent with a hierarchy of asperity and segment sizes that radiate waves with different tensorial compositions and frequency bands. While different deformation processes dominating parts of the rupture zones can be treated effectively with existing constitutive relations, a more appropriate analysis of earthquake processes would require a model that combines aspects of fracture, damage-breakage, and frictional frameworks.
On 12 August 2021, a > 220 s lasting complex earthquake with Mw > 8.2 hit the South Sandwich Trench. Due to its remote location and short interevent times, reported earthquake parameters varied significantly between different international agencies. We studied the complex rupture by combining different seismic source characterization techniques sensitive to different frequency ranges based on teleseismic broadband recordings from 0.001 to 2 Hz, including point and finite fault inversions and the back-projection of high-frequency signals.
We also determined moment tensor solutions for 88 aftershocks. The rupture initiated simultaneously with a rupture equivalent to a M-w 7.6 thrust earthquake in the deep part of the seismogenic zone in the central subduction interface and a shallow megathrust rupture, which propagated unilaterally to the south with a very slow rupture velocity of 1.2 km/s and varying strike following the curvature of the trench.
The slow rupture covered nearly two-thirds of the entire subduction zone length, and with M-w 8.2 released the bulk of the total moment of the whole earthquake.
Tsunami modeling indicates the inferred shallow rupture can explain the tsunami records. The southern segment of the shallow rupture overlaps with another activation of the deeper part of the megathrust equivalent to M-w 7.6. The aftershock distribution confirms the extent and curvature of the rupture. Some mechanisms are consistent with the mainshocks, but many indicate also activation of secondary faults. Rupture velocities and radiated frequencies varied strongly between different stages of the rupture, which might explain the variability of published source parameters.
Plain Language Summary
The earthquake of 12 August 2021 along the deep-sea trench of the South Sandwich Islands in the South Atlantic reached a magnitude of 8.2 and triggered a tsunami. The automatic earthquake parameter determination of different agencies showed very different results shortly after the earthquake and partially underestimated the tsunami potential of the earthquake.
A possible reason was the complex rupture process and that the tsunami was generated by a long and shallow slow slip rupture sandwiched between more conventional fast slip subevents at its northern and southern ends. In addition, the fault surface, which extended over 450 km, was highly curved striking 150 degrees-220 degrees.
We investigated the different components of the seismic wavefields in different frequency ranges and with different methods.
The analysis shows how even complex earthquakes can be deciphered by combining analyzing methods. The comparison with aftershocks and the triggered tsunami waves confirms our model that explains the South Sandwich rupture by four subevents in the plate boundary along the curved deep-sea trench. Here, the depth, rupture velocities, and slip on each segment of the rupture vary considerably.
The method can also be applied to other megathrust earthquakes and help to further improve tsunami warnings in the future.
The ambitious climate targets set by industrialized nations worldwide cannot be met without decarbonizing the building stock.
Using Germany as a case study, this paper takes stock of the extensive set of energy efficiency policies that are already in place and clarifies that they have been designed "in good faith" but lack in overall effectiveness as well as cost-efficiency in achieving these climate targets.
We map out the market failures and behavioural considerations that are potential reasons for why realized energy savings fall below expectations and why the household adoption of energy-efficient and low-carbon technologies has remained low. We highlight the pressing need for data and modern empirical research to develop targeted and cost-effective policies seeking to correct these market failures.
To this end, we identify some key research questions and identify gaps in the data required for evidence-based policy.
Hematology, hormones, inflammation, and muscle damage in elite and professional soccer players
(2021)
Background
Knowledge of the long-term effects of soccer training on hematological, hormonal, inflammatory, and muscle damage markers and physical performance may help to better design strength and conditioning programs for performance development and injury prevention for the individual player and the team.
Objectives
The aim of this systematic review was to summarize and discuss evidence on the long-term effects of soccer training on selected hematological, hormonal, inflammatory, and muscle damage markers and physical performance in elite and professional soccer players. A second goal was to investigate associations between selected physiological markers and measures of performance.
Methods
Adhering to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines, a systematic literature search was conducted in four electronic databases (PubMed, ISI Web of Knowledge, Web of Science, and SPORTDiscus) from inception until August 2020 to identify articles related to soccer training effects. To be included in this systematic review, studies had to examine male elite (national level) and/or professional (international level) soccer players aged > 17 years and a soccer training period > 4 weeks, and report outcomes related to hematological, hormonal, inflammatory, muscle damage, and performance markers.
Results
The search syntax initially identified 2420 records. After screening titles, abstracts, and full texts, 20 eligible studies were included in this systematic review, with training durations lasting between 4 and 18 weeks in 15 studies, around 6 months in four studies, and around 1 year in one study. Effects of long-term soccer training revealed parameter-specific increases or decreases in hematological, hormonal, inflammatory, and muscle damage markers and physical performance. Two studies showed a moderate increase in hematological markers such as hemoglobin (effect size [ES] = 0.67-0.93). Parameter-specific changes were noted for hormonal markers in the form of increases for total testosterone (ES = 0.20-0.67) and free testosterone (FT) (ES = 0.20-0.65) and decreases for cortisol (ES = - 0.28 to - 1.31). Finally, moderate to very large increases were found for muscle damage markers such as creatine kinase (ES = 0.94-6.80) and physical performance such as countermovement jump (CMJ) height (ES = 0.50-1.11) and squat jump (SJ) height (ES = 0.65-1.28). After long-term periods of soccer training, significant positive correlations were found between percentage change (Delta%) in FT and Delta% in CMJ height (r = 0.94; p = 0.04) and between Delta% in total testosterone/cortisol (TT/C) ratio and Delta% in SJ (r = 0.89; p = 0.03).
Conclusions
Findings suggest that long-term soccer training induces increases/decreases in hematological, hormonal, inflammatory, and muscle damage markers and physical performance in male elite and professional soccer players. These fluctuations can be explained by different contextual factors (e.g., training load, duration of training, psychological factors, mood state). Interestingly, the observed changes in hormonal parameters (FT and TT/C) were related to vertical jump performance changes (e.g., CMJ and SJ). Anabolic hormones and TT/C can possibly be used as a tool to identify physical performance alteration after long-term soccer training.
Background:
The current range of disease-modifying treatments (DMTs) for relapsing-remitting multiple sclerosis (RRMS) has placed more importance on the accurate monitoring of disease progression for timely and appropriate treatment decisions. With a rising number of measurements for disease progression, it is currently unclear how well these measurements or combinations of them can monitor more mildly affected RRMS patients.
Objectives:
To investigate several composite measures for monitoring disease activity and their potential relation to the biomarker neurofilament light chain (NfL) in a clearly defined early RRMS patient cohort with a milder disease course.
Methods:
From a total of 301 RRMS patients, a subset of 46 patients being treated with a continuous first-line therapy was analyzed for loss of no evidence of disease activity (lo-NEDA-3) status, relapse-associated worsening (RAW) and progression independent of relapse activity (PIRA), up to seven years after treatment initialization.
Kaplan-Meier estimates were used for time-to-event analysis. Additionally, a Cox regression model was used to analyze the effect of NIL levels on outcome measures in this cohort.
Results:
In this mildly affected cohort, both lo-NEDA-3 and PIRA frequently occurred over a median observational period of 67.2 months and were observed in 39 (84.8%) and 23 (50.0%) patients, respectively.
Additionally, 12 out of 26 PIRA manifestations (46.2%) were observed without a corresponding lo-NEDA-3 status. Jointly, either PIRA or lo-NEDA-3 showed disease activity in all patients followed-up for at least the median duration (67.2 months). NfL values demonstrated an association with the occurrence of relapses and RAW.
Conclusion:
The complementary use of different disease progression measures helps mirror ongoing disease activity in mildly affected early RRMS patients being treated with continuous first-line therapy.
This book provides a novel analysis for the syntax of the clausal left periphery, focusing on various finite clause types and especially on embedded clauses. It investigates how the appearance of multiple projections interacts with economy principles and with the need for marking syntactic information overtly. In particular, the proposed account shows that a flexible approach assuming only a minimal number of projections is altogether favourable to cartographic approaches. The main focus of the book is on West Germanic, in particular on English and German, yet other Germanic and non-Germanic languages are also discussed for comparative purposes.
Rapid innovation and proliferation of software as a medical device have accelerated the clinical use of digital technologies across a wide array of medical conditions.
Current regulatory pathways were developed for traditional (hardware) medical devices and offer a useful structure, but the evolution of digital devices requires concomitant innovation in regulatory approaches to maximize the potential benefits of these emerging technologies.
A number of specific adaptations could strengthen current regulatory oversight while promoting ongoing innovation.
New middle Miocene to Pliocene (~14–3 Ma) apatite fission track (AFT) cooling ages combined with published K–Ar/Ar–Ar and zircon fission track (ZFT) ages from the Hazara and Swat regions of Pakistan are used to explain the Oligocene to Pliocene structural evolution in the Western Himalaya. The structural model explains the distribution of K–Ar/Ar–Ar ages in three distinct age groups (Proterozoic, Paleozoic-Mesozoic, and Eocene to Oligocene). The Proterozoic to Mesozoic sequence of northern Hazara and Swat experienced elevated temperature and pressure conditions, evident by reset Eocene to Oligocene K–Ar/Ar–Ar hornblende and Eocene to Miocene muscovite ages, caused by Kohistan overthrusting the Indian margin during and after the India–Asia collision. Samples from the Indus syntaxis with Paleo to Mesoproterozoic K–Ar/Ar–Ar hornblende ages and Eocene to Oligocene Ar–Ar muscovite ages show no signs of Cenozoic metamorphism; these samples were thermally imprinted up to the Ar–Ar muscovite closure temperature. Neoproterozoic to Lower Paleozoic rocks from the southern parts of Hazara and Swat show Mesozoic to Oligocene partially reset Ar–Ar muscovite ages and preservation of Ordovician metamorphism. The combined analysis of published K–Ar/Ar–Ar (muscovite), ZFT, and new AFT ages (~14–12 Ma) suggests that the Main Central thrust/Panjal thrust was active from Oligocene to early Miocene (~30–18 Ma), and the Nathia-Gali and Main Boundary thrusts were active from the middle to late Miocene (~14–9 Ma) in the Hazara area. New and published AFT ages (~6–3 Ma) from the Indus syntaxis suggest that early Pliocene tectonic thickening in the hinterland formed the N–S trending Indus anticline, creating an erosional half window in the Main Mantle thrust, forming the Indus syntaxis, and dividing the Main Central thrust sheet into the Hazara and Swat segments.
Localized surface plasmon resonances on noble metal nanoparticles (NPs) can efficiently drive reactions of adsorbed ligand molecules and provide versatile opportunities in chemical synthesis. The driving forces of these reactions are typically elevated temperatures, hot charge carriers, or enhanced electric fields.
In the present work, dehalogenation of halogenated thiophenols on the surface of AuNPs has been studied by surface enhanced Raman scattering (SERS) as a function of the photon energy to track the kinetics and identify reaction products.
Reaction rates are found to be surprisingly similar for different halothiophenols studied here, although the bond dissociation energies of the C-X bonds differ significantly. Complementary information about the electronic properties at the AuNP surface, namely, work-function and valence band states, has been determined by x-ray photoelectron spectroscopy of isolated AuNPs in the gas-phase.
In this way, it is revealed how the electronic properties are altered by the adsorption of the ligand molecules, and we conclude that the reaction rates are mainly determined by the plasmonic properties of the AuNPs. SERS spectra reveal differences in the reaction product formation for different halogen species, and, on this basis, the possible reaction mechanisms are discussed to approach an understanding of opportunities and limitations in the design of catalytical systems with plasmonic NPs.
Microviridins are a prominent family of ribosomally synthesized and posttranslationally modified peptides (RiPPs) featuring characteristic lactone and lactam rings. Their unusual cage-like architecture renders them highly potent serine protease inhibitors of which individual variants specifically inhibit different types of proteases of pharmacological interest.
While posttranslational modifications are key for the stability and bioactivity of RiPPs, additional attractive properties can be introduced by functional tags.
To date - although highly desirable - no method has been reported to incorporate functional tags in microviridin scaffolds or the overarching class of graspetides.
In this study, a chemoenzymatic in vitro platform is used to introduce functional tags in various microviridin variants yielding biotinylated, dansylated or propargylated congeners.
This straightforward approach paves the way for customized protease inhibitors with built-in functionalities that can help to unravel the still elusive ecological roles and targets of this remarkable class of compounds and to foster applications based on protease inhibition.
Recent theories suggest a shift from model-based goal-directed to model-free habitual decision-making in obsessive-compulsive disorder (OCD). However, it is yet unclear, whether this shift in the decision process is heritable. We investigated 32 patients with OCD, 27 unaffected siblings (SIBs) and 31 healthy controls (HCs) using the two-step task. We computed behavioral and reaction time analyses and fitted a computational model to assess the balance between model-based and model-free control. 80 subjects also underwent structural imaging. We observed a significant ordered effect for the shift towards model-free control in the direction OCD>SIB>HC in our computational parameter of interest. However less directed analyses revealed no shift towards model-free control in OCDs. Nonetheless, we found evidence for reduced model-based control in OCDs compared to HCs and SIBs via 2nd stage reaction time analyses. In this measure SIBs also showed higher levels of model-based control than HCs. Across all subjects these effects were associated with the surface area of the left medial/right dorsolateral prefrontal cortex. Moreover, correlations between bilateral putamen/right caudate volumes and these effects varied as a function of group: they were negative in SIBs and OCDs, but positive in HCs. Associations between fronto-striatal regions and model-based reaction time effects point to a potential endophenotype for OCD.
We propose lacunarity as a novel recurrence quantification measure and illustrate its efficacy to detect dynamical regime transitions which are exhibited by many complex real-world systems. We carry out a recurrence plot-based analysis for different paradigmatic systems and nonlinear empirical data in order to demonstrate the ability of our method to detect dynamical transitions ranging across different temporal scales. It succeeds to distinguish states of varying dynamical complexity in the presence of noise and non-stationarity, even when the time series is of short length. In contrast to traditional recurrence quantifiers, no specification of minimal line lengths is required and geometric features beyond linear structures in the recurrence plot can be accounted for. This makes lacunarity more broadly applicable as a recurrence quantification measure. Lacunarity is usually interpreted as a measure of heterogeneity or translational invariance of an arbitrary spatial pattern. In application to recurrence plots, it quantifies the degree of heterogeneity in the temporal recurrence patterns at all relevant time scales. We demonstrate the potential of the proposed method when applied to empirical data, namely time series of acoustic pressure fluctuations from a turbulent combustor. Recurrence lacunarity captures both the rich variability in dynamical complexity of acoustic pressure fluctuations and shifting time scales encoded in the recurrence plots. Furthermore, it contributes to a better distinction between stable operation and near blowout states of combustors.
Like many other regions in central Europe, Germany experienced sequential summer droughts from 2015 to 2018. As one of the environmental consequences, river nitrate concentrations have exhibited significant changes in many catchments.
However, catchment nitrate responses to the changing weather conditions have not yet been mechanistically explored.
Thus, a fully distributed, process-based catchment Nitrate model (mHM-Nitrate) was used to reveal the causal relations in the Bode catchment, of which river nitrate concentrations have experienced contrasting trends from upstream to downstream reaches. The model was evaluated using data from six gauging stations, reflecting different levels of runoff components and their associated nitrate-mixing from upstream to downstream.
Results indicated that the mHM-Nitrate model reproduced dynamics of daily discharge and nitrate concentration well, with Nash-Sutcliffe Efficiency >= 0.73 for discharge and Kling-Gupta Efficiency >= 0.50 for nitrate concentration at most stations.
Particularly, the spatially contrasting trends of nitrate con-centration were successfully captured by the model.
The decrease of nitrate concentration in the lowland area in drought years (2015-2018) was presumably due to (1) limited terrestrial export loading (ca. 40 % lower than that of normal years 2004-2014), and (2) increased in-stream retention efficiency (20 % higher in summer within the whole river network).
From a mechanistic modelling perspective, this study provided insights into spatially heterogeneous flow and nitrate dynamics and effects of sequential droughts, which shed light on water -quality responses to future climate change, as droughts are projected to be more frequent.
An epilepsy diagnosis has large consequences for an individual but is often difficult to make in clinical practice.
Novel biomarkers are thus greatly needed. Here, we give an overview of how thousands of common genetic factors that increase the risk for epilepsy can be summarized as epilepsy polygenic risk scores (PRS).
We discuss the current state of research on how epilepsy PRS can serve as a biomarker for the risk for epilepsy. The high heritability of common forms of epilepsy, particularly genetic generalized epilepsy, indicates a promising potential for epilepsy PRS in diagnosis and risk prediction.
Small sample sizes and low ancestral diversity of current epilepsy genome-wide association studies show, however, a need for larger and more diverse studies before epilepsy PRS could be properly implemented in the clinic.
Fichte’s Wissenschaftslehre of 1801/2 is considered to be the beginning of his late phase. In this phase he supposedly alters his earlier thinking and, instead of the transcendental unity of the I, conceptualizes a higher transcendent and simple unity; a unity that has been claimed to correspond to Neoplatonism. I refute these two arguments here. First, through a comparison between the Wissenschaftslehre of 1801/2 and that of 1794/5, I show that both versions contain a similar analysis of the supreme unity. Second, I show that in 1801/2 Fichte explicitly dissociates the supreme unity from transcendence and simplicity. His conception of the supreme unity in fact levels a critique upon such concept of unity. Instead of the transcendent One, which is hierarchically prior to multiplicity, Fichte formulates in both 1794/5 and 1801/2 a complicated concept of the supreme unity. On Fichte’s account, this unity “hovers” between multiplicity and unity as simplicity.
Fichte claims in Section 5 of the Grundlage der gesamten Wissenschaftslehre (GWL) that the absolute I contains a difference between two directions. In this paper, I argue that this specific claim complements, rather than contradicts, his general position in Section 1, according to which the absolute I is a simple identity or a point. I first show that we can identify a version of what I call Fichte’s Two-Directions Theory in texts written both before and after the GWL. I term this version his Two-Series Theory. Drawing on this reading, I demonstrate, in contrast to previous research, that Fichte consistently formulates the absolute I in a twofold manner: as a pure act that precedes any difference and as a proposition that contains a relation. I further argue that Fichte’s treatment of the absolute I as a simple unity and as a unity of two directions maps onto two stages within his philosophical investigation. On my account, Fichte seeks to comprehend the absolute I by considering the I’s activity both as it is in itself and as it presents itself to thought.
In this topical review, we give an overview of the structure and dynamics of a single polymer chain in active baths, Gaussian or non-Gaussian.
The review begins with the discussion of single flexible or semiflexible linear polymer chains subjected to two noises, thermal and active.
The active noise has either Gaussian or non-Gaussian distribution but has a memory, accounting for the persistent motion of the active bath particles. This finite persistence makes the reconfiguration dynamics of the chain slow as compared to the purely thermal case and the chain swells.
The active noise also results superdiffusive or ballistic motion of the tagged monomer. We present all the calculations in details but mainly focus on the analytically exact or almost exact results on the topic, as obtained from our group in recent years.
In addition, we briefly mention important works of other groups and include some of our new results. The review concludes with pointing out the implications of polymer chains in active bath in biologically relevant context and its future directions.
We introduce the concept of TRAP (Traces and Permutations), which can roughly be viewed as a wheeled PROP (Products and Permutations) without unit. TRAPs are equipped with a horizontal concatenation and partial trace maps.
Continuous morphisms on an infinite-dimensional topological space and smooth kernels (respectively, smoothing operators) on a closed manifold form a TRAP but not a wheeled PROP.
We build the free objects in the category of TRAPs as TRAPs of graphs and show that a TRAP can be completed to a unitary TRAP (or wheeled PROP).
We further show that it can be equipped with a vertical concatenation, which on the TRAP of linear homomorphisms of a vector space, amounts to the usual composition. The vertical concatenation in the TRAP of smooth kernels gives rise to generalised convolutions.
Graphs whose vertices are decorated by smooth kernels (respectively, smoothing operators) on a closed manifold form a TRAP. From their universal properties we build smooth amplitudes associated with the graph.
Studies investigating the effect of aboveground herbivory on plants often use clipping to simulate the effects of herbivores, for practical reasons. However, herbivore movements and transfer of oral secretions during herbivory may cause a different response in plant physiology and morphology compared to clipping.
While studies have compared effects of real herbivory vs. clipping on biomass production, plant physiology, and shoot morphology, no study has compared such effects on root morphology.
Therefore, we investigated the effect of herbivory by grasshoppers, herbivory simulated by clipping, and no herbivory on root morphological traits of ten grassland plant species. Root morphological traits were differently affected by the two herbivory treatments. Grasshopper herbivory significantly changed root morphology toward thinner roots with increased specific root length and root area, and decreased root tissue density compared to untreated control plants.
Clipping had mostly similar, but weaker effects on root morphology than grasshopper herbivory.
On the species level, grasshopper herbivory led to strongest changes in root morphology in almost all cases. In contrast, depending on the species, clipping resulted in varying root morphological trait values similar to grasshopper-damaged plants, or in some cases, more closely aligned with control plants.
Though clipping was partly able to mimic the effects of herbivory by grasshoppers, results also indicate that, depending on the species, grasshopper herbivory had different but mostly stronger effects. We, therefore, recommend that future studies apply herbivory with real herbivores to better reflect natural responses in plants and related processes that root morphological traits mediate.
In the Science of Logic, Hegel criticizes Fichte's Wissenschaftslehre for advancing from the beginning through external reflection and thus failing to understand both the nature of the beginning and the proper method to advance from it. This article shows that Fichte's advance from the beginning preempts Hegel's critique and shares Hegel's premises with respect to the method of advancing. The author first analyzes Hegel's critique of Fichte in the Science of Logic, which he follows by showing that Fichte levels a similar critique against Schelling's conception of the beginning set out in his system of 1801. Turning to the Wissenschaftslehre of 1794/5, the author then demonstrates that Fichte's method of advancing from the beginning addresses every point of Hegel's critique. He concludes by identifying the similarity and difference between Fichte's and Hegel's methods of advancing.