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The Role of the Nucleotides in the Insertion of the bis-Molybdopterin Guanine Dinucleotide Cofactor into apo-Molybdoenzymes
- The role of the GMP nucleotides of the bis-molybdopterin guanine dinucleotide (bis-MGD) cofactor of the DMSO reductase family has long been a subject of discussion. The recent characterization of the bis-molybdopterin (bis-Mo-MPT) cofactor present in the E. coli YdhV protein, which differs from bis-MGD solely by the absence of the nucleotides, now enables studying the role of the nucleotides of bis-MGD and bis-MPT cofactors in Moco insertion and the activity of molybdoenzymes in direct comparison. Using the well-known E. coli TMAO reductase TorA as a model enzyme for cofactor insertion, we were able to show that the GMP nucleotides of bis-MGD are crucial for the insertion of the bis-MGD cofactor into apo-TorA.
Author details: | Kim Tiedemann, Chantal Iobbi-NivolORCiD, Silke LeimkühlerORCiDGND |
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DOI: | https://doi.org/10.3390/molecules27092993 |
ISSN: | 1420-3049 |
Title of parent work (English): | Molecules |
Publisher: | MDPI |
Place of publishing: | Basel, Schweiz |
Further contributing person(s): | Roberto Fernandez-Lafuente |
Publication type: | Article |
Language: | English |
Date of first publication: | 2022/05/06 |
Publication year: | 2022 |
Release date: | 2022/09/27 |
Tag: | TMAO reductase; bis-MGD; chaperone; molybdenum cofactor |
Volume: | 27 |
Article number: | 2993 |
Print run: | 9 |
Number of pages: | 15 |
First page: | 1 |
Last Page: | 15 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Extern / Extern | |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer review: | Referiert |
Grantor: | Publikationsfonds der Universität Potsdam |
Publishing method: | Open Access / Gold Open-Access |
License (German): | CC-BY - Namensnennung 4.0 International |
External remark: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 1268 |