Starch granule size and morphology of Arabidopsis thaliana starch-related mutants analyzed during diurnal rhythm and development
- Transitory starch plays a central role in the life cycle of plants. Many aspects of this important metabolism remain unknown; however, starch granules provide insight into this persistent metabolic process. Therefore, monitoring alterations in starch granules with high temporal resolution provides one significant avenue to improve understanding. Here, a previously established method that combines LCSM and safranin-O staining for in vivo imaging of transitory starch granules in leaves of Arabidopsis thaliana was employed to demonstrate, for the first time, the alterations in starch granule size and morphology that occur both throughout the day and during leaf aging. Several starch-related mutants were included, which revealed differences among the generated granules. In ptst2 and sex1-8, the starch granules in old leaves were much larger than those in young leaves; however, the typical flattened discoid morphology was maintained. In ss4 and dpe2/phs1/ss4, the morphology of starch granules in young leaves was altered, with a moreTransitory starch plays a central role in the life cycle of plants. Many aspects of this important metabolism remain unknown; however, starch granules provide insight into this persistent metabolic process. Therefore, monitoring alterations in starch granules with high temporal resolution provides one significant avenue to improve understanding. Here, a previously established method that combines LCSM and safranin-O staining for in vivo imaging of transitory starch granules in leaves of Arabidopsis thaliana was employed to demonstrate, for the first time, the alterations in starch granule size and morphology that occur both throughout the day and during leaf aging. Several starch-related mutants were included, which revealed differences among the generated granules. In ptst2 and sex1-8, the starch granules in old leaves were much larger than those in young leaves; however, the typical flattened discoid morphology was maintained. In ss4 and dpe2/phs1/ss4, the morphology of starch granules in young leaves was altered, with a more rounded shape observed. With leaf development, the starch granules became spherical exclusively in dpe2/phs1/ss4. Thus, the presented data provide new insights to contribute to the understanding of starch granule morphogenesis.…
Author details: | Qingting Liu, Yuan Zhou, Jörg FettkeORCiDGND |
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DOI: | https://doi.org/10.3390/molecules26195859 |
ISSN: | 1420-3049 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/34641402 |
Title of parent work (English): | Molecules : a journal of synthetic chemistry and natural product chemistry / Molecular Diversity Preservation International |
Publisher: | MDPI |
Place of publishing: | Basel, Schweiz |
Further contributing person(s): | Janusz Kapusniak, Idalina Gonçalves |
Publication type: | Article |
Language: | English |
Date of first publication: | 2021/09/27 |
Publication year: | 2021 |
Release date: | 2022/05/25 |
Tag: | Arabidopsis thaliana; LCSM; starch granule; starch granule morphology; starch granule size; starch metabolism |
Volume: | 26 |
Article number: | 5859 |
Print run: | 19 |
Number of pages: | 9 |
First page: | 1 |
Last Page: | 9 |
Funding institution: | Deutsche Forschungsgemeinschaft DFG, German Research Foundation (DFG) |
Funding number: | FE 1030/2-1, FE 1030/5-1, FE 1030/6-1 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Extern / Extern | |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer review: | Referiert |
Grantor: | Publikationsfonds der Universität Potsdam |
Publishing method: | Open Access / Gold Open-Access |
License (German): | CC-BY - Namensnennung 4.0 International |
External remark: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 1245 |