- Since its discovery over two decades ago as an important cell death regulator in Arabidopsis thaliana, the role of LESION SIMULATING DISEASE 1 (LSD1) has been studied intensively within both biotic and abiotic stress responses as well as with respect to plant fitness regulation. However, its molecular mode of action remains enigmatic. Here, we demonstrate that nucleo-cytoplasmic LSD1 interacts with a broad range of other proteins that are engaged in various molecular pathways such as ubiquitination, methylation, cell cycle control, gametogenesis, embryo development and cell wall formation. The interaction of LSD1 with these partners is dependent on redox status, as oxidative stress significantly changes the quantity and types of LSD1-formed complexes. Furthermore, we show that LSD1 regulates the number and size of leaf mesophyll cells and affects plant vegetative growth. Importantly, we also reveal that in addition to its function as a scaffold protein, LSD1 acts as a transcriptional regulator. Taken together, our results demonstrateSince its discovery over two decades ago as an important cell death regulator in Arabidopsis thaliana, the role of LESION SIMULATING DISEASE 1 (LSD1) has been studied intensively within both biotic and abiotic stress responses as well as with respect to plant fitness regulation. However, its molecular mode of action remains enigmatic. Here, we demonstrate that nucleo-cytoplasmic LSD1 interacts with a broad range of other proteins that are engaged in various molecular pathways such as ubiquitination, methylation, cell cycle control, gametogenesis, embryo development and cell wall formation. The interaction of LSD1 with these partners is dependent on redox status, as oxidative stress significantly changes the quantity and types of LSD1-formed complexes. Furthermore, we show that LSD1 regulates the number and size of leaf mesophyll cells and affects plant vegetative growth. Importantly, we also reveal that in addition to its function as a scaffold protein, LSD1 acts as a transcriptional regulator. Taken together, our results demonstrate that LSD1 plays a dual role within the cell by acting as a condition-dependent scaffold protein and as a transcription regulator.…
MetadatenVerfasserangaben: | Weronika CzarnockaORCiD, Katrien Van Der Kelen, Patrick Willems, Magdalena Szechynska-Hebda, Sara Shahnejat-Bushehri, Salma BalazadehORCiDGND, Anna Rusaczonek, Bernd Müller-RöberORCiDGND, Frank Van Breusegem, Stanislaw Karpinski |
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DOI: | https://doi.org/10.1111/pce.12994 |
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ISSN: | 0140-7791 |
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ISSN: | 1365-3040 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/28555890 |
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Titel des übergeordneten Werks (Englisch): | Plant, cell & environment : cell physiology, whole-plant physiology, community physiology |
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Verlag: | Wiley |
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Verlagsort: | Hoboken |
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Publikationstyp: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Jahr der Erstveröffentlichung: | 2017 |
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Erscheinungsjahr: | 2017 |
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Datum der Freischaltung: | 20.04.2020 |
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Freies Schlagwort / Tag: | Arabidopsis; LSD1; dry weight; oxidative stress; protein interaction; thaliana; transcription regulation |
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Band: | 40 |
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Seitenanzahl: | 19 |
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Erste Seite: | 2644 |
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Letzte Seite: | 2662 |
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Fördernde Institution: | Programme [FP7-REGPOT-2012-1-286093]; FWO project [G0D7914N]; WOODTECH project - National Centre for Research and Development [PBS1/A8/16/2013]; Biostrateg (CROPTECH) project - National Centre for Research and Development [298241]; Foundation for Polish Science [START didactic approach to the question of how to obtain raw plant materials for the purposes of energy production in the context of the Europe |
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Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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Peer Review: | Referiert |
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