Investigation of the post mortem zinc protoporphyrin IX fluorescence with respect to its protein-bound and unbound occurrence in aqueous meat extracts
- Zinc protoporphyrin IX (ZnPP) is known to accumulate in most meat products during storage. However, the pathway of its formation is not yet completely clarified. To gain more insights into the specificity of ZnPP occurrence, a SEC-HPLC-UV-fluorescence setup was established to screen the proteins in aqueous meat extracts for their ZnPP fluorescence during incubation. In accordance with previous studies it was identified by SDS-PAGE and MALDI-TOF-MS that ZnPP formation takes place in myoglobin. In this study, valuable new insights into the ZnPP forming pathway were gained, as our results indicated that a significant part of ZnPP - after being formed within the protein - is transitioned into free ZnPP during incubation. Additionally, the obtained results implied that ZnPP may also occur in proteins of higher molecular weight (> 100 kDa).
Verfasserangaben: | Amin Ghadiri KhozroughiGND, Tess Waldbach Braga, Janine Wagner, Harshadrai Manilal RawelORCiDGND |
---|---|
DOI: | https://doi.org/10.1016/j.foodchem.2019.01.080 |
ISSN: | 0308-8146 |
ISSN: | 1873-7072 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30722899 |
Titel des übergeordneten Werks (Englisch): | Food chemistry |
Verlag: | Elsevier |
Verlagsort: | Oxford |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2019 |
Erscheinungsjahr: | 2019 |
Datum der Freischaltung: | 21.01.2021 |
Freies Schlagwort / Tag: | Fluorescence screening; MALDI-TOF-MS; Meat; Post mortem chemistry; SDS-PAGE; SEC-HPLC |
Band: | 283 |
Seitenanzahl: | 6 |
Erste Seite: | 462 |
Letzte Seite: | 467 |
Fördernde Institution: | Avicenna foundation from the Federal Ministry of Education and Research of Germany |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Peer Review: | Referiert |
Publikationsweg: | Open Access / Bronze Open-Access |