Characterization of the enhanced peroxidatic activity of amyloid beta peptide-hemin complexes towards neurotransmitters
- Binding of heme to the amyloid peptides A beta 40/42 is thought to be an initial step in the development of symptoms in the early stages of Alzheimer's disease by enhancing the intrinsic peroxidatic activity of heme. We found considerably higher acceleration of the reaction for the physiologically relevant neurotransmitters dopamine and serotonin than reported earlier for the artificial substrate 3,3',5,5'-tetramethylbenzidine (TMB). Thus, the binding of hemin to A beta peptides might play an even more crucial role in the early stages of Alzheimer's disease than deduced from these earlier results. To mimic complex formation, a new surface architecture has been developed: The interaction between the truncated amyloid peptide A beta 1-16 and hemin immobilized on an aminohexanethiol spacer on a gold electrode has been analyzed by cyclic voltammetry. The resulting complex has a redox pair with a 25 mV more cathodic formal potential than hemin alone.
Verfasserangaben: | Bettina NeumannORCiD, Aysu YarmanORCiDGND, Ursula WollenbergerORCiDGND, Frieder W. SchellerORCiDGND |
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DOI: | https://doi.org/10.1007/s00216-014-7822-8 |
ISSN: | 1618-2642 |
ISSN: | 1618-2650 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/24760399 |
Titel des übergeordneten Werks (Englisch): | Analytical & bioanalytical chemistry |
Verlag: | Springer |
Verlagsort: | Heidelberg |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2014 |
Erscheinungsjahr: | 2014 |
Datum der Freischaltung: | 27.03.2017 |
Freies Schlagwort / Tag: | Peroxidatic activity |
Band: | 406 |
Ausgabe: | 14 |
Seitenanzahl: | 6 |
Erste Seite: | 3359 |
Letzte Seite: | 3364 |
Fördernde Institution: | BMBF of Germany [0311993]; Deutsche Forschungsgemeinschaft (DFG) [EXC 314] |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer Review: | Referiert |