Microfluidic tools for quantitative studies of eukaryotic chemotaxis
- Over the past decade, microfluidic techniques have been established as a versatile platform to perform live cell experiments under well-controlled conditions. To investigate the directional responses of cells, stable concentration profiles of chemotactic factors can be generated in microfluidic gradient mixers that provide a high degree of spatial control. However, the times for built-up and switching of gradient profiles are in general too slow to resolve the intracellular protein translocation events of directional sensing of eukaryotes. Here, we review an example of a conventional microfluidic gradient mixer as well as the novel flow photolysis technique that achieves an increased temporal resolution by combining the photo-activation of caged compounds with the advantages of microfluidic chambers.
Author details: | Carsten BetaORCiDGND, Eberhard Bodenschatz |
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DOI: | https://doi.org/10.1016/j.ejcb.2011.05.006 |
ISSN: | 0171-9335 |
Title of parent work (English): | European journal of cell biology |
Publisher: | Elsevier |
Place of publishing: | Jena |
Publication type: | Review |
Language: | English |
Year of first publication: | 2011 |
Publication year: | 2011 |
Release date: | 2017/03/26 |
Tag: | Caged compounds; Dictyostelium discoideum; Eukaryotic chemotaxis; Microfluidics; Numerical simulations |
Volume: | 90 |
Issue: | 10 |
Number of pages: | 6 |
First page: | 811 |
Last Page: | 816 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Physik und Astronomie |
Peer review: | Referiert |