How reliable is the electrochemical readout of MIP-sensors?
- Electrochemical methods offer the simple characterization of the synthesis of molecularly imprinted polymers (MIPs) and the readouts of target binding. The binding of electroinactive analytes can be detected indirectly by their modulating effect on the diffusional permeability of a redox marker through thin MIP films. However, this process generates an overall signal, which may include nonspecific interactions with the nonimprinted surface and adsorption at the electrode surface in addition to (specific) binding to the cavities. Redox-active low-molecular-weight targets and metalloproteins enable a more specific direct quantification of their binding to MIPs by measuring the faradaic current. The in situ characterization of enzymes, MIP-based mimics of redox enzymes or enzyme-labeled targets, is based on the indication of an electroactive product. This approach allows the determination of both the activity of the bio(mimetic) catalyst and of the substrate concentration.
Author details: | Aysu YarmanORCiDGND, Frieder W. SchellerORCiDGND |
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URN: | urn:nbn:de:kobv:517-opus4-471608 |
DOI: | https://doi.org/10.25932/publishup-47160 |
ISSN: | 1866-8372 |
Title of parent work (German): | Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe |
Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (960) |
Publication type: | Postprint |
Language: | English |
Date of first publication: | 2020/06/23 |
Publication year: | 2020 |
Publishing institution: | Universität Potsdam |
Release date: | 2020/06/23 |
Tag: | catalysis; direct electron transfer; electropolymerization; gate effect; molecularly imprinted polymers; redox marker |
Issue: | 960 |
Number of pages: | 25 |
Source: | Sensors 20 (2020) 9, 2677 DOI:10.3390/s20092677 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät |
DDC classification: | 6 Technik, Medizin, angewandte Wissenschaften / 62 Ingenieurwissenschaften / 620 Ingenieurwissenschaften und zugeordnete Tätigkeiten |
Peer review: | Referiert |
Publishing method: | Open Access |
License (German): | CC-BY - Namensnennung 4.0 International |