In vitro transcription of a whole gene on a surface-coupled template
- An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated
| Author details: | Jenny Steffen, Markus von Nickisch-RosenegkORCiD, Frank Fabian BierORCiDGND |
|---|---|
| ISSN: | 1473-0189 |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2005 |
| Publication year: | 2005 |
| Release date: | 2017/03/24 |
| Source: | Lab on a Chip. - ISSN 1473-0189. - 5 (2005), 6, S. 665 - 668 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
