Applying CLSM to increment core surfaces for histometric analyses a novel advance in quantitative wood anatomy

A novel procedure has been developed to conduct cell structure measurements on increment core samples of conifers. The procedure combines readily available hardware and software equipment. The essential part of the procedure is the application of a confocal laser scanning microscope (CLSM) which captures images directly from increment cores surfaced with the advanced WSL core-microtome. Cell wall and lumen are displayed with a strong contrast due to the monochrome black and green nature of the images. Consecutive images are merged into long images representing entire increment cores which are then analysed for cell structures in suitable software.

Metadaten
Verfasserangaben:	Wei Liang, Ingo Heinrich, Gerhard Helle, Isabel Dorado Linan, Thilo Heinken ORCiD
DOI:	https://doi.org/10.1016/j.dendro.2012.09.002
ISSN:	1125-7865
Titel des übergeordneten Werks (Englisch):	Dendrochronologia : an interdisciplinary journal of tree-ring science
Verlag:	Elsevier
Verlagsort:	Jena
Publikationstyp:	Wissenschaftlicher Artikel
Sprache:	Englisch
Jahr der Erstveröffentlichung:	2013
Erscheinungsjahr:	2013
Datum der Freischaltung:	26.03.2017
Freies Schlagwort / Tag:	CLSM; Cell structures; Confocal laser scanning microscopy; Dendrochronology; Surface preparation; Wood anatomy
Band:	31
Ausgabe:	2
Seitenanzahl:	6
Erste Seite:	140
Letzte Seite:	145
Fördernde Institution:	Federal Ministry of Education and Research (BMBF); Helmholtz Association of German Research Centres Initiative (Networking Fund); Helmholtz - CSC Junior Scientists Exchange Program by the Helmholtz Association of German Research Centres; China Scholarship Council (CSC)
Organisationseinheiten:	Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer Review:	Referiert