The search result changed since you submitted your search request. Documents might be displayed in a different sort order.
  • search hit 4 of 144
Back to Result List

Lipopolysaccharides (LPS) Induced Angiogenesis During Chicken Embryogenesis is Abolished by Combined ETA/ETB Receptor Blockade

  • Background/Aims: Angiogenesis plays a key role during embryonic development. The vascular endothelin (ET) system is involved in the regulation of angiogenesis. Lipopolysaccharides (LPS) could induce angiogenesis. The effects of ET blockers on baseline and LPS-stimulated angiogenesis during embryonic development remain unknown so far. Methods: The blood vessel density (BVD) of chorioallantoic membranes (CAMs), which were treated with saline (control), LPS, and/or BQ123 and the ETB blocker BQ788, were quantified and analyzed using an IPP 6.0 image analysis program. Moreover, the expressions of ET-1, ET-2, ET3, ET receptor A (ETRA), ET receptor B (ETRB) and VEGFR2 mRNA during embryogenesis were analyzed by semi-quantitative RT-PCR. Results: All components of the ET system are detectable during chicken embryogenesis. LPS increased angiogenesis substantially. This process was completely blocked by the treatment of a combination of the ETA receptor blockers-BQ123 and the ETB receptor blocker BQ788. This effect was accompanied by a decreaseBackground/Aims: Angiogenesis plays a key role during embryonic development. The vascular endothelin (ET) system is involved in the regulation of angiogenesis. Lipopolysaccharides (LPS) could induce angiogenesis. The effects of ET blockers on baseline and LPS-stimulated angiogenesis during embryonic development remain unknown so far. Methods: The blood vessel density (BVD) of chorioallantoic membranes (CAMs), which were treated with saline (control), LPS, and/or BQ123 and the ETB blocker BQ788, were quantified and analyzed using an IPP 6.0 image analysis program. Moreover, the expressions of ET-1, ET-2, ET3, ET receptor A (ETRA), ET receptor B (ETRB) and VEGFR2 mRNA during embryogenesis were analyzed by semi-quantitative RT-PCR. Results: All components of the ET system are detectable during chicken embryogenesis. LPS increased angiogenesis substantially. This process was completely blocked by the treatment of a combination of the ETA receptor blockers-BQ123 and the ETB receptor blocker BQ788. This effect was accompanied by a decrease in ETRA, ETRB, and VEGFR2 gene expression. However, the baseline angiogenesis was not affected by combined ETA/ETB receptor blockade. Conclusion: During chicken embryogenesis, the LPS-stimulated angiogenesis, but not baseline angiogenesis, is sensitive to combined ETA/ETB receptor blockade. (C) 2018 The Author(s) Published by S. Karger AG, Baselshow moreshow less

Download full text files

  • pmnr615.pdfeng
    (2854KB)

    SHA-1: f0ad4f9a1470f8df6c00d33e9e780446ee848c2f

Export metadata

Additional Services

Search Google Scholar Statistics
Metadaten
Author details:Guang Wang, Pei-zhi Li, Shi-yao Zhang, Shan Zhong, Chang ChuORCiD, Shufei Zeng, Yu Yan, Xin Cheng, Manli Chuai, Berthold HocherORCiDGND, Xuesong Yang
URN:urn:nbn:de:kobv:517-opus4-424552
DOI:https://doi.org/10.1159/000492547
ISSN:1866-8372
Title of parent work (English):Cellular Physiology and Biochemistry
Publication series (Volume number):Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (615)
Publication type:Postprint
Language:English
Date of first publication:2019/02/18
Publication year:2018
Publishing institution:Universität Potsdam
Release date:2019/02/18
Tag:Angiogenesis; Chicken chorioallantoic membrane (CAM); Endothelin (ET); Lipopolysaccharides (LPS)
Issue:615
Number of pages:7
Source:Cellular Physiology and Biochemistry 48 (2018) pp. 2084-2090 DOI: 10.1159/000492547
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät
DDC classification:5 Naturwissenschaften und Mathematik
Peer review:Referiert
Publishing method:Open Access
License (German):License LogoCC-BY-NC-ND - Namensnennung, nicht kommerziell, keine Bearbeitungen 4.0 International
External remark:Bibliographieeintrag der Originalveröffentlichung/Quelle
Accept ✔
This website uses technically necessary session cookies. By continuing to use the website, you agree to this. You can find our privacy policy here.