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An ion-controlled four-color fluorescent telomeric switch on DNA origami structures
- The folding of single-stranded telomeric DNA into guanine (G) quadruplexes is a conformational change that plays a major role in sensing and drug targeting. The telomeric DNA can be placed on DNA origami nanostructures to make the folding process extremely selective for K+ ions even in the presence of high Na+ concentrations. Here, we demonstrate that the K+-selective G-quadruplex formation is reversible when using a cryptand to remove K+ from the G-quadruplex. We present a full characterization of the reversible switching between single-stranded telomeric DNA and G-quadruplex structures using Förster resonance energy transfer (FRET) between the dyes fluorescein (FAM) and cyanine3 (Cy3). When attached to the DNA origami platform, the G-quadruplex switch can be incorporated into more complex photonic networks, which is demonstrated for a three-color and a four-color FRET cascade from FAM over Cy3 and Cy5 to IRDye700 with G-quadruplex-Cy3 acting as a switchable transmitter.
Author details: | Lydia OlejkoORCiDGND, Piotr J. Cywiński, Ilko BaldORCiDGND |
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DOI: | https://doi.org/10.1039/C6NR00119J |
ISSN: | 2040-3372 |
ISSN: | 2040-3364 |
Title of parent work (English): | Nanoscale |
Publisher: | RSC Publ. |
Place of publishing: | Cambridge |
Publication type: | Article |
Language: | English |
Date of first publication: | 2016/04/15 |
Publication year: | 2016 |
Publishing institution: | Universität Potsdam |
Release date: | 2016/09/15 |
Tag: | 3-color fret; assemblies; g-quadruplex; nanostructures; photonic wires; quantum dots; recognition; resonance energy-transfer; sensitivity; strand breakage |
Volume: | 8 |
First page: | 10339 |
Last Page: | 10347 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer review: | Referiert |
Publishing method: | Open Access |
Grantor: | RSC |
License (English): | Creative Commons - Namensnennung 3.0 Unported |
External remark: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 254 |