Microwave-Assisted Synthesis of 5 '-O-methacryloylcytidine Using the Immobilized Lipase Novozym 435
- Nucleobase building blocks have been demonstrated to be strong candidates when it comes to DNA/RNA-like materials by benefiting from hydrogen bond interactions as physical properties. Modifying at the 5 ' position is the simplest way to develop nucleobase-based structures by transesterification using the lipase Novozym 435. Herein, we describe the optimization of the lipase-catalyzed synthesis of the monomer 5 '-O-methacryloylcytidine with the assistance of microwave irradiation. Variable reaction parameters, such as enzyme concentration, molar ratio of the substrate, reaction temperature and reaction time, were investigated to find the optimum reaction condition in terms of obtaining the highest yield.
| Author details: | Sany CheaORCiDGND, Khac Toan NguyenORCiD, Ruben R. RosencrantzORCiDGND |
|---|---|
| DOI: | https://doi.org/10.3390/molecules27134112 |
| ISSN: | 1420-3049 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/35807358 |
| Title of parent work (English): | Molecules |
| Publisher: | MDPI |
| Place of publishing: | Basel |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2022/06/26 |
| Publication year: | 2022 |
| Release date: | 2024/01/03 |
| Tag: | Novozym 435; cytidine; microwave irradiation; monomer; smart materials |
| Volume: | 27 |
| Issue: | 13 |
| Article number: | 4112 |
| Number of pages: | 11 |
| Funding institution: | Fraunhofer Internal Programs [Discover 840 205] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Peer review: | Referiert |
| Publishing method: | Open Access / Gold Open-Access |
| DOAJ gelistet | |
| License (German): |  CC-BY - Namensnennung 4.0 International |
