Polydopamine-based biofunctional substrate coating promotes mesenchymal stem cell migration
- Rapid migration of mesenchymal stem cells (MSCs) on device surfaces could support in vivo tissue integration and might facilitate in vitro organoid formation. Here, polydopamine (PDA) is explored as a biofunctional coating to effectively promote MSC motility. It is hypothesized that PDA stimulates fibronectin deposition and in this way enhances integrin-mediated migration capability. The random and directional cell migration was investigated by time-lapse microscopy and gap closure assay respectively, and analysed with softwares as computational tools. A higher amount of deposited fibronectin was observed on PDA substrate, compared to the non-coated substrate. The integrin beta 1 activation and focal adhesion kinase (FAK) phosphorylation at Y397 were enhanced on PDA substrate, but the F-actin cytoskeleton was not altered, suggesting MSC migration on PDA was regulated by integrin initiated FAK signalling. This study strengthens the biofunctionality of PDA coating for regulating stem cells and offering a way of facilitating tissueRapid migration of mesenchymal stem cells (MSCs) on device surfaces could support in vivo tissue integration and might facilitate in vitro organoid formation. Here, polydopamine (PDA) is explored as a biofunctional coating to effectively promote MSC motility. It is hypothesized that PDA stimulates fibronectin deposition and in this way enhances integrin-mediated migration capability. The random and directional cell migration was investigated by time-lapse microscopy and gap closure assay respectively, and analysed with softwares as computational tools. A higher amount of deposited fibronectin was observed on PDA substrate, compared to the non-coated substrate. The integrin beta 1 activation and focal adhesion kinase (FAK) phosphorylation at Y397 were enhanced on PDA substrate, but the F-actin cytoskeleton was not altered, suggesting MSC migration on PDA was regulated by integrin initiated FAK signalling. This study strengthens the biofunctionality of PDA coating for regulating stem cells and offering a way of facilitating tissue integration of devices.…
| Author details: | Zijun DengGND, Weiwei WangGND, Xun Xu, Nan Ma, Andreas LendleinORCiDGND |
|---|---|
| DOI: | https://doi.org/10.1557/s43580-021-00091-4 |
| ISSN: | 2059-8521 |
| Title of parent work (English): | MRS advances : a journal of the Materials Research Society (MRS) |
| Publisher: | Springer Nature Switzerland AG |
| Place of publishing: | Cham |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2021/06/30 |
| Publication year: | 2021 |
| Release date: | 2024/04/29 |
| Volume: | 6 |
| Issue: | 31 |
| Number of pages: | 6 |
| First page: | 739 |
| Last Page: | 744 |
| Funding institution: | Helmholtz Association of German Research CentersHelmholtz Association; Federal Ministry of Education and Research, GermanyFederal Ministry of Education & Research (BMBF) [13GW0098]; I2B Funds (Project: high-resolution imaging and computational analysis to study the dynamics of stem cell-biomaterial interaction) |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Peer review: | Referiert |
| Publishing method: | Open Access / Hybrid Open-Access |
| License (German): |  CC-BY - Namensnennung 4.0 International |
