A competitive immunoassay to detect a hapten using an enzyme-labelled peptide mimotope as tracer
- Mimotope peptides-peptides which mimic the binding of a hapten to its corresponding monoclonal antibody-were conjugated to peroxidase and used in competitive immunoassay. The established immunoassay was used to quantitatively determine the concentration of hapten. As model system in all the experiments described here, we used the binding of the monoclonal antibody B13-DE1 to fluorescein and the corresponding peptide mimotope.
| Author details: | Frank SellrieGND, Jörg A. SchenkORCiD, Olaf Behrsing, Volker Bottger, Burkhard Micheel |
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| URL: | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2Y-44MGNDF- 1&_coverDate=03%2F01%2F2002&_alid=268992656&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=4931&_sort=d&view=c&_acct=C000053886&_v e |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2002 |
| Publication year: | 2002 |
| Release date: | 2017/03/24 |
| Source: | Journal of Immunological Methods. - 261 (2002), 1-2, S. 141 - 144 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
