Low-energy (5-20 eV) electron-induced single and double strand breaks in well-defined DNA sequences
- Ionizing radiation is used in cancer radiation therapy to effectively damage the DNA of tumors. The main damage is due to generation of highly reactive secondary species such as low-energy electrons (LEEs). The accurate quantification of DNA radiation damage of well-defined DNA target sequences in terms of absolute cross sections for LEE-induced DNA strand breaks is possible by the DNA origami technique; however, to date, it is possible only for DNA single strands. In the present work DNA double strand breaks in the DNA sequence 5 '-d(CAC)4/5 ' d(GTG)4 are compared with DNA single strand breaks in the oligonucleotides 5 '-d(CAC)4 and 5 '-d(GTG)4 upon irradiation with LEEs in the energy range from 5 to 20 eV. A maximum of strand break cross section was found around 7 and 10 eV independent of the DNA sequence, indicating that dissociative electron attachment is the underlying mechanism of strand breakage and confirming previous studies using plasmid DNA.
Author details: | Kenny EbelORCiDGND, Ilko BaldORCiDGND |
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DOI: | https://doi.org/10.1021/acs.jpclett.2c00684 |
ISSN: | 1948-7185 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/35617198 |
Title of parent work (English): | Journal of physical chemistry letters |
Publisher: | American Chemical Society |
Place of publishing: | Washington, DC |
Publication type: | Article |
Language: | English |
Date of first publication: | 2022/05/26 |
Publication year: | 2022 |
Release date: | 2024/07/22 |
Volume: | 13 |
Issue: | 22 |
Number of pages: | 6 |
First page: | 4871 |
Last Page: | 4876 |
Funding institution: | European Research Council (ERC) [772752]; German Research Foundation; (DFG) [230710387] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
Peer review: | Referiert |
Publishing method: | Open Access / Hybrid Open-Access |
License (German): | CC-BY-NC-ND - Namensnennung, nicht kommerziell, keine Bearbeitungen 4.0 International |