Cryo-Electron microscopy for the study of self-assembled poly(ionic liquid) nanoparticles and protein supramolecular structures
- Cryo-electron microscopy (cryo-EM) is a powerful structure determination technique that is well-suited to the study of protein and polymer self-assembly in solution. In contrast to conventional transmission electron microscopy (TEM) sample preparation, which often times involves drying and staining, the frozen-hydrated sample preparation allows the specimens to be kept and imaged in a state closest to their native one. Here, we give a short overview of the basic principles of Cryo-EM and review our results on applying it to the study of different protein and polymer self-assembled nanostructures. More specifically, we show how we have applied cryo-electron tomography (cryo-ET) to visualize the internal morphology of self-assembled poly(ionic liquid) nanoparticles and cryo-EM single particle analysis (SPA) to determine the three-dimensional (3D) structures of artificial protein microtubules.
Author details: | Zdravko KochovskiORCiD, Guosong Chen, Jiayin YuanORCiDGND, Yan LuORCiDGND |
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DOI: | https://doi.org/10.1007/s00396-020-04657-w |
ISSN: | 0303-402X |
ISSN: | 1435-1536 |
Title of parent work (English): | Colloid and polymer science : official journal of the Kolloid-Gesellschaft |
Publisher: | Springer |
Place of publishing: | New York |
Publication type: | Article |
Language: | English |
Date of first publication: | 2020/05/23 |
Publication year: | 2020 |
Release date: | 2022/09/02 |
Tag: | cryo-electron; cryo-electron microscopy; poly(ionic liquid) nanoparticles; protein self-assembly; self-assembly; single particle analysis; tomography |
Volume: | 298 |
Issue: | 7 |
Number of pages: | 11 |
First page: | 707 |
Last Page: | 717 |
Funding institution: | Projekt DEAL; Deutsche Forschungsgemeinschaft (DFG, German Research; Foundation)German Research Foundation (DFG) [LU1423/1-1] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer review: | Referiert |
Publishing method: | Open Access / Hybrid Open-Access |
License (German): | CC-BY - Namensnennung 4.0 International |