- Treffer 1 von 1
Bacteria differently regulate mRNA abundance to specifically respond to various stresses
- Environmental stress is detrimental to cell viability and requires an adequate reprogramming of cellular activities to maximize cell survival. We present a global analysis of the response of Escherichia coli to acute heat and osmotic stress. We combine deep sequencing of total mRNA and ribosome-protected fragments to provide a genome-wide map of the stress response at transcriptional and translational levels. For each type of stress, we observe a unique subset of genes that shape the stress-specific response. Upon temperature upshift, mRNAs with reduced folding stability up-and downstream of the start codon, and thus with more accessible initiation regions, are translationally favoured. Conversely, osmotic upshift causes a global reduction of highly translated transcripts with high copy numbers, allowing reallocation of translation resources to not degraded and newly synthesized mRNAs.
Verfasserangaben: | Alexander BartholomäusORCiDGND, Ivan Fedyunin, Peter Feist, Celine Sin, Gong Zhang, Angelo Valleriani, Zoya Ignatova |
---|---|
DOI: | https://doi.org/10.1098/rsta.2015.0069 |
ISSN: | 1364-503X |
ISSN: | 1471-2962 |
Titel des übergeordneten Werks (Englisch): | Geology |
Verlag: | Royal Society |
Verlagsort: | London |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2016 |
Erscheinungsjahr: | 2016 |
Datum der Freischaltung: | 22.03.2020 |
Freies Schlagwort / Tag: | Escherichia coli; copy numbers; deep sequencing; transcription; translation |
Band: | 374 |
Seitenanzahl: | 16 |
Fördernde Institution: | Deutsche Forschungsgemeinschaft; European Union (ITN NICHE) |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer Review: | Referiert |