Lectin binding studies on a glycopolymer brush flow-through biosensor by localized surface plasmon resonance

A localized surface plasmon resonance biosensor in a flow-through configuration was applied for investigating kinetics of lectin binding to surface-grafted glycopolymer brushes. Polycarbonate filter membranes with pore sizes of 400 nm were coated with a 114-nm thick gold layer and used as substrate for surface-initiated atom-transfer radical polymerization of a glycomonomer. These grafted from glycopolymer brushes were further modified with two subsequent enzymatic reactions on the surface to yield an immobilized trisaccharide presenting brush. Specific binding of lectins including Clostridium difficile toxin A receptor domain to the glycopolymer brush surface could be investigated in a microfluidic setup with flow-through of the analytes and transmission surface plasmon resonance spectroscopy.

Metadaten
Author details:	Ruben R. Rosencrantz ORCiD GND, Vu Hoa Nguyen, Hyunji Park, Christine Schulte, Alexander Böker ORCiD GND, Uwe Schnakenberg, Lothar Elling
DOI:	https://doi.org/10.1007/s00216-016-9667-9
ISSN:	1618-2642
ISSN:	1618-2650
Pubmed ID:	https://pubmed.ncbi.nlm.nih.gov/27277814
Title of parent work (English):	Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry and Analusis
Publisher:	Springer
Place of publishing:	Heidelberg
Publication type:	Article
Language:	English
Year of first publication:	2016
Publication year:	2016
Release date:	2020/03/22
Tag:	Bacterial toxin; Biosensors; Glycopolymer brush; Glycosyltransferase; Localized surface plasmon resonance; Microfluidics
Volume:	408
Number of pages:	8
First page:	5633
Last Page:	5640
Funding institution:	Deutsche Forschungsgemeinschaft (DFG) [SFB 985]
Organizational units:	Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer review:	Referiert