An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants
- A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen- sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong auto fluorescence of the plant tissue. The method was established using photosynthesis- competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species
| Verfasserangaben: | E. Schmalzlin, J. T. van Dongen, I. Klimant, Bettina Marmodee, Martin SteupORCiDGND, Joachim Fisahn, Peter Ludwig Geigenberger, Hans-Gerd LöhmannsröbenORCiDGND |
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| ISSN: | 0006-3495 |
| Publikationstyp: | Wissenschaftlicher Artikel |
| Sprache: | Englisch |
| Jahr der Erstveröffentlichung: | 2005 |
| Erscheinungsjahr: | 2005 |
| Datum der Freischaltung: | 24.03.2017 |
| Quelle: | Biophysical Journal. - ISSN 0006-3495. - 89 (2005), 2, S. 1339 - 1345 |
| Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer Review: | Referiert |
