Wei-Hong Wang, Barbara Köhler, Feng-Qiu Cao, Guo-Wei Liu, Yuan-Yong Gong, Song Sheng, Qi-Chao Song, Xiao-Yuan Cheng, Trevor Garnett, Mamoru Okamoto, Rui Qin, Bernd Müller-Röber, Mark Tester, Lai-Hua Liu
- Despite the great agricultural and ecological importance of efficient use of urea-containing nitrogen fertilizers by crops, molecular and physiological identities of urea transport in higher plants have been investigated only in Arabidopsis. We performed short-time urea-influx assays which have identified a low-affinity and high-affinity (Km of 7.55 mu M) transport system for urea-uptake by rice roots (Oryza sativa). A high-affinity urea transporter OsDUR3 from rice was functionally characterized here for the first time among crops. OsDUR3 encodes an integral membrane-protein with 721 amino acid residues and 15 predicted transmembrane domains. Heterologous expression demonstrated that OsDUR3 restored yeast dur3-mutant growth on urea and facilitated urea import with a Km of c. 10 mu M in Xenopus oocytes. Quantitative reverse-transcription polymerase chain reaction (qPCR) analysis revealed upregulation of OsDUR3 in rice roots under nitrogen-deficiency and urea-resupply after nitrogen-starvation. Importantly, overexpression of OsDUR3Despite the great agricultural and ecological importance of efficient use of urea-containing nitrogen fertilizers by crops, molecular and physiological identities of urea transport in higher plants have been investigated only in Arabidopsis. We performed short-time urea-influx assays which have identified a low-affinity and high-affinity (Km of 7.55 mu M) transport system for urea-uptake by rice roots (Oryza sativa). A high-affinity urea transporter OsDUR3 from rice was functionally characterized here for the first time among crops. OsDUR3 encodes an integral membrane-protein with 721 amino acid residues and 15 predicted transmembrane domains. Heterologous expression demonstrated that OsDUR3 restored yeast dur3-mutant growth on urea and facilitated urea import with a Km of c. 10 mu M in Xenopus oocytes. Quantitative reverse-transcription polymerase chain reaction (qPCR) analysis revealed upregulation of OsDUR3 in rice roots under nitrogen-deficiency and urea-resupply after nitrogen-starvation. Importantly, overexpression of OsDUR3 complemented the Arabidopsis atdur3-1 mutant, improving growth on low urea and increasing root urea-uptake markedly. Together with its plasma membrane localization detected by green fluorescent protein (GFP)-tagging and with findings that disruption of OsDUR3 by T-DNA reduces rice growth on urea and urea uptake, we suggest that OsDUR3 is an active urea transporter that plays a significant role in effective urea acquisition and utilisation in rice.…
MetadatenAuthor details: | Wei-Hong Wang, Barbara Köhler, Feng-Qiu Cao, Guo-Wei Liu, Yuan-Yong Gong, Song Sheng, Qi-Chao Song, Xiao-Yuan Cheng, Trevor Garnett, Mamoru Okamoto, Rui Qin, Bernd Müller-RöberORCiDGND, Mark Tester, Lai-Hua Liu |
---|
DOI: | https://doi.org/10.1111/j.1469-8137.2011.03929.x |
---|
ISSN: | 0028-646X |
---|
Title of parent work (English): | New phytologist : international journal of plant science |
---|
Publisher: | Wiley-Blackwell |
---|
Place of publishing: | Malden |
---|
Publication type: | Article |
---|
Language: | English |
---|
Year of first publication: | 2012 |
---|
Publication year: | 2012 |
---|
Release date: | 2017/03/26 |
---|
Tag: | OsDUR3; high-affinity transporter; leaf senescence; nitrogen remobilization; overexpression; rice plant; urea transport and utilization |
---|
Volume: | 193 |
---|
Issue: | 2 |
---|
Number of pages: | 13 |
---|
First page: | 432 |
---|
Last Page: | 444 |
---|
Funding institution: | NHTRD, China [2006AA10Z166]; NSF of China (NSFC) [30771288, 31070223];
NSFC [30821003] |
---|
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
---|
Peer review: | Referiert |
---|