Miniature direct electron transfer based sulphite/oxygen enzymatic fuel cells
- A direct electron transfer (DET) based sulphite/oxygen biofuel cell is reported that utilises human sulphite oxidase (hSOx) and Myrothecium verrucaria bilirubin oxidase (MvBOx) and nanostructured gold electrodes. For bioanode construction, the nanostructured gold microelectrodes were further modified with 3,3'-dithiodipropionic acid di(N-hydroxysuccinimide ester) to which polyethylene imine was covalently attached. hSOx was adsorbed onto this chemically modified nanostructured electrode with high surface loading of electroactive enzyme and in presence of sulphite high anodic bioelectrocatalytic currents were generated with an onset potential of 0.05 V vs. NHE. The biocathode contained MyBOx directly adsorbed to the deposited gold nanoparticles for cathodic oxygen reduction starting at 0.71 V vs. NHE. Both enzyme electrodes were integrated to a DET-type biofuel cell. Power densities of 8 and 1 mu W cm(-2) were achieved at 0.15 V and 0.45 V of cell voltages, respectively, with the membrane based biodevices under aerobic conditions. (C)A direct electron transfer (DET) based sulphite/oxygen biofuel cell is reported that utilises human sulphite oxidase (hSOx) and Myrothecium verrucaria bilirubin oxidase (MvBOx) and nanostructured gold electrodes. For bioanode construction, the nanostructured gold microelectrodes were further modified with 3,3'-dithiodipropionic acid di(N-hydroxysuccinimide ester) to which polyethylene imine was covalently attached. hSOx was adsorbed onto this chemically modified nanostructured electrode with high surface loading of electroactive enzyme and in presence of sulphite high anodic bioelectrocatalytic currents were generated with an onset potential of 0.05 V vs. NHE. The biocathode contained MyBOx directly adsorbed to the deposited gold nanoparticles for cathodic oxygen reduction starting at 0.71 V vs. NHE. Both enzyme electrodes were integrated to a DET-type biofuel cell. Power densities of 8 and 1 mu W cm(-2) were achieved at 0.15 V and 0.45 V of cell voltages, respectively, with the membrane based biodevices under aerobic conditions. (C) 2014 Elsevier B.V. All rights reserved.…
| Author details: | Ting Zeng, Dmitry Pankratov, Magnus Falk, Silke LeimkühlerORCiDGND, Sergey Shleev, Ursula WollenbergerORCiDGND |
|---|---|
| DOI: | https://doi.org/10.1016/j.bios.2014.10.080 |
| ISSN: | 0956-5663 |
| ISSN: | 1873-4235 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/25460879 |
| Title of parent work (English): | Biosensors and bioelectronics : the principal international journal devoted to research, design development and application of biosensors and bioelectronics |
| Publisher: | Elsevier |
| Place of publishing: | Oxford |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2015 |
| Publication year: | 2015 |
| Release date: | 2017/03/27 |
| Tag: | Bilirubin oxidase; Direct electron transfer; Enzymatic fuel cell; Microscale electrode; Sulphite oxidase |
| Volume: | 66 |
| Number of pages: | 4 |
| First page: | 39 |
| Last Page: | 42 |
| Funding institution: | Russian Scientific Foundation [14-14-00530]; Deutsche Forschungsgemeinschaft (Unicat Cluster of Excellence) [EXC 314/2]; ILB-Brandenburg [80136126]; BMBF [03IS2201B] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
