Photocontrol of Coiled-Coil Proteins in Living Cells
- Light switching of the activity of a coiled-coil protein, the AP-1 transcription factor, in living cells was made possible by the introduction of a designed azobenzene-cross-linked dominant negative peptide, XAFosW (red and yellow in the picture). In the dark, XAFosW showed decreased helical content and decreased affinity for target Jun proteins (green); irradiation at 365 nm enhanced helicity and target affinity.
| Author details: | Fuzhong Z. Zhang, Katharina A. Timm, Katja Maren ArndtORCiDGND, G. Andrew Woolley |
|---|---|
| URL: | http://www3.interscience.wiley.com/cgi-bin/jhome/26737/ |
| DOI: | https://doi.org/10.1002/anie.201000909 |
| ISSN: | 1433-7851 |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2010 |
| Publication year: | 2010 |
| Release date: | 2017/03/25 |
| Source: | Angewandte Chemie : international edition. - ISSN 1433-7851. - 49 (2010), 23, S. 3943 - 3946 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
