Cell-substrate impedance fluctuations of single amoeboid cells encode cell-shape and adhesion dynamics

We show systematic electrical impedance measurements of single motile cells on microelectrodes. Wild-type cells and mutant strains were studied that differ in their cell-substrate adhesion strength. We recorded the projected cell area by time-lapse microscopy and observed irregular oscillations of the cell shape. These oscillations were correlated with long-term variations in the impedance signal. Superposed to these long-term trends, we observed fluctuations in the impedance signal. Their magnitude clearly correlated with the adhesion strength, suggesting that strongly adherent cells display more dynamic cell-substrate interactions.

Metadaten
Verfasserangaben:	Helmar Leonhardt, Matthias Gerhardt, Nadine Hoeppner, Kirsten Krüger, Marco Tarantola, Carsten Beta ORCiD GND
DOI:	https://doi.org/10.1103/PhysRevE.93.012414
ISSN:	2470-0045
ISSN:	2470-0053
Pubmed ID:	https://pubmed.ncbi.nlm.nih.gov/26871108
Titel des übergeordneten Werks (Englisch):	Physical review : E, Statistical, nonlinear and soft matter physics
Verlag:	American Physical Society
Verlagsort:	College Park
Publikationstyp:	Wissenschaftlicher Artikel
Sprache:	Englisch
Jahr der Erstveröffentlichung:	2016
Erscheinungsjahr:	2016
Datum der Freischaltung:	22.03.2020
Band:	93
Seitenanzahl:	8
Fördernde Institution:	Ministerium fur Wissenschaft, Forschung und Kultur des Landes Brandenburg; Europaischer Fond fur regionale Entwicklung (EFRE) [80147097, 80148364, F111-02-UNIP/003]; Deutsche Forschungsgemeinschaft [BE 3978/3-1, SFB 937]
Organisationseinheiten:	Mathematisch-Naturwissenschaftliche Fakultät / Institut für Physik und Astronomie
Peer Review:	Referiert