In vitro transcription of a whole gene on a surface-coupled template
- An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated
| Verfasserangaben: | Jenny Steffen, Markus von Nickisch-RosenegkORCiD, Frank Fabian BierORCiDGND |
|---|---|
| ISSN: | 1473-0189 |
| Publikationstyp: | Wissenschaftlicher Artikel |
| Sprache: | Englisch |
| Jahr der Erstveröffentlichung: | 2005 |
| Erscheinungsjahr: | 2005 |
| Datum der Freischaltung: | 24.03.2017 |
| Quelle: | Lab on a Chip. - ISSN 1473-0189. - 5 (2005), 6, S. 665 - 668 |
| Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer Review: | Referiert |
