Lectin binding studies on a glycopolymer brush flow-through biosensor by localized surface plasmon resonance
- A localized surface plasmon resonance biosensor in a flow-through configuration was applied for investigating kinetics of lectin binding to surface-grafted glycopolymer brushes. Polycarbonate filter membranes with pore sizes of 400 nm were coated with a 114-nm thick gold layer and used as substrate for surface-initiated atom-transfer radical polymerization of a glycomonomer. These grafted from glycopolymer brushes were further modified with two subsequent enzymatic reactions on the surface to yield an immobilized trisaccharide presenting brush. Specific binding of lectins including Clostridium difficile toxin A receptor domain to the glycopolymer brush surface could be investigated in a microfluidic setup with flow-through of the analytes and transmission surface plasmon resonance spectroscopy.
| Verfasserangaben: | Ruben R. RosencrantzORCiDGND, Vu Hoa Nguyen, Hyunji Park, Christine Schulte, Alexander BökerORCiDGND, Uwe Schnakenberg, Lothar Elling |
|---|---|
| DOI: | https://doi.org/10.1007/s00216-016-9667-9 |
| ISSN: | 1618-2642 |
| ISSN: | 1618-2650 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/27277814 |
| Titel des übergeordneten Werks (Englisch): | Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry and Analusis |
| Verlag: | Springer |
| Verlagsort: | Heidelberg |
| Publikationstyp: | Wissenschaftlicher Artikel |
| Sprache: | Englisch |
| Jahr der Erstveröffentlichung: | 2016 |
| Erscheinungsjahr: | 2016 |
| Datum der Freischaltung: | 22.03.2020 |
| Freies Schlagwort / Tag: | Bacterial toxin; Biosensors; Glycopolymer brush; Glycosyltransferase; Localized surface plasmon resonance; Microfluidics |
| Band: | 408 |
| Seitenanzahl: | 8 |
| Erste Seite: | 5633 |
| Letzte Seite: | 5640 |
| Fördernde Institution: | Deutsche Forschungsgemeinschaft (DFG) [SFB 985] |
| Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer Review: | Referiert |
