Detecting carbohydrate-lectin interactions using a fluorescent probe based on DBD dyes
- Herein we present an efficient synthesis of a biomimetic probe with modular construction that can be specifically bound by the mannose binding FimH protein - a surface adhesion protein of E. coli bacteria. The synthesis combines the new and interesting DBD dye with the carbohydrate ligand mannose via a Click reaction. We demonstrate the binding to E. coli bacteria over a large concentration range and also present some special characteristics of those molecules that are of particular interest for the application as a biosensor. In particular, the mix-and-measure ability and the very good photo-stability should be highlighted here.
| Author details: | Denise Bader, Dennis Tobias KlierGND, C. Hettrich, Frank Fabian BierORCiDGND, Pablo WessigORCiDGND |
|---|---|
| DOI: | https://doi.org/10.1039/c5ay02991k |
| ISSN: | 1759-9660 |
| ISSN: | 1759-9679 |
| Title of parent work (English): | Analytical methods : advancing methods and applications |
| Publisher: | Royal Society of Chemistry |
| Place of publishing: | Cambridge |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2016 |
| Publication year: | 2016 |
| Release date: | 2020/03/22 |
| Volume: | 8 |
| Number of pages: | 4 |
| First page: | 1235 |
| Last Page: | 1238 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
| Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie | |
| Peer review: | Referiert |
