Detection of angiotensin II type 1 receptor ligands by a cell-based assay
- This work describes a cell-based assay that does not depend on radioactivity or laboratory animals for the detection of ligands of angiotensin II type 1 receptor (AT(1)R). The assay makes use of stable transfected Chinese hamster ovary cells (CHO-AT(1)R) expressing the AT(1)R. A sequential saturation assay principle was used in which receptor binding sites of the CHO-AT(1)R cells are blocked by the analyte in a concentration-dependent manner. Afterwards, TAMRA-angiotensin II, a fluorescence-labeled ligand, was added to bind to the remaining free binding sites of the receptor. In consequence, the fluorescence signal determined is inversely proportional to the concentration of the analyte.
Author details: | Matthias Grießner, Patrick Broeker, André Lehmann, Eva Ehrentreich-Förster, Frank Fabian BierORCiDGND |
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URL: | http://www.springerlink.com/content/100417 |
DOI: | https://doi.org/10.1007/s00216-009-3074-4 |
ISSN: | 1618-2642 |
Publication type: | Article |
Language: | English |
Year of first publication: | 2009 |
Publication year: | 2009 |
Release date: | 2017/03/25 |
Source: | Analytical and bioanalytical chemistry. - ISSN 1618-2642. - 395 (2009), 6, S. 1937 - 1940 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer review: | Referiert |