Characterization of a new maleimido functionalization of gold for surface plasmon resonance spectroscopy
- Para-maleimidophenyl (p-MP) modified gold surfaces have been prepared by one-step electrochemical deposition and used in surface plasmon resonance (SPR) studies. Therefore, a FITC mimotope peptide (MP1, 12 aa), a human mucin 1 epitope peptide (MUC, 9 aa) and a protein with their specific antibodies were used as model systems. The peptides were modified with an N-terminal cysteine for covalent and directed coupling to the maleimido functionalized surface by means of Michael addition. The coupling yield of the peptide, the binding characteristics of antibody and the unspecific adsorption of the analytes were investigated. The results expand the spectrum of biosensors usable with p-MP by widely used SPR and support its potential to be versatile for several electrochemical and optical biosensors. This allows the combination of an electrochemical and optical read-out for a broad variety of biomolecular interactions on the same chip. Copyright (c) 2014 John Wiley & Sons, Ltd.
Author details: | Marc Hovestaedt, Henry MemczakGND, Dennis Pleiner, Xin Zhang, Joerg Rappich, Frank Fabian BierORCiDGND, Walter F. M. Stöcklein |
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DOI: | https://doi.org/10.1002/jmr.2396 |
ISSN: | 0952-3499 |
ISSN: | 1099-1352 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/25319618 |
Title of parent work (English): | Journal of molecular recognition : an international journal devoted to research on specific molecular recognition in chemistry, biology, biotechnology and medicine |
Publisher: | Wiley-Blackwell |
Place of publishing: | Hoboken |
Publication type: | Article |
Language: | English |
Year of first publication: | 2014 |
Publication year: | 2014 |
Release date: | 2017/03/27 |
Tag: | aryl diazonium salts; biosensor; cys-peptide; diazonium coupling; maleimidophenyl; surface plasmon resonance |
Volume: | 27 |
Issue: | 12 |
Number of pages: | 7 |
First page: | 707 |
Last Page: | 713 |
Funding institution: | Federal Ministry of Science and Education (BMBF), Germany [FKZ 03 IS2201 A] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer review: | Referiert |