Sortase-mediated ligation of purely artificial building blocks
- Sortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP-polymer, NP-NP, and polymer-polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction-the conserved peptide LPETG-and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in casesSortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP-polymer, NP-NP, and polymer-polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction-the conserved peptide LPETG-and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated.…
Verfasserangaben: | Xiaolin DaiORCiDGND, Diana M. Mate, Ulrich GlebeORCiDGND, Tayebeh Mirzaei Garakani, Andrea Körner, Ulrich SchwanebergORCiD, Alexander BökerORCiDGND |
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DOI: | https://doi.org/10.3390/polym10020151 |
ISSN: | 2073-4360 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30966187 |
Titel des übergeordneten Werks (Englisch): | Polymers |
Verlag: | MDPI |
Verlagsort: | Basel |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Erstveröffentlichung: | 06.02.2018 |
Erscheinungsjahr: | 2018 |
Datum der Freischaltung: | 26.01.2022 |
Freies Schlagwort / Tag: | block copolymers; enzymes; nanoparticles; sortase-mediated ligation |
Band: | 10 |
Ausgabe: | 2 |
Seitenanzahl: | 13 |
Fördernde Institution: | Alexander von Humboldt-StiftungAlexander von Humboldt Foundation; EUEuropean Union (EU); federal state of North Rhine-Westphali [EFRE 30 00 883 02] |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer Review: | Referiert |
Publikationsweg: | Open Access / Gold Open-Access |
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