Toxin-antigen conjugates as selection tools for antibody producing cells
- The generation of antibodies with designated specificity requires cost-intensive and time-consuming screening procedures. Here we present a new method by which hybridoma cells can be selected based on the specificity of the produced antibody by the use of antigen-toxin-conjugates thus eliminating the need of a screening procedure. Initial experiments were done with methotrexate as low molecular weight toxin and fluorescein as model antigen. Methotrexate and a methotrexate-fluorescein conjugate were characterized regarding their toxicity. Afterwards the effect of the fluorescein-specific antibody B13-DE1 on the toxicity of the methotrexate-fluorescein conjugate was determined. Finally, first results showed that hybridoma cells that produce fluorescein specific antibodies are able to grow in the presence of fluorescein-toxin-conjugates.
Author details: | Katrin MesserschmidtORCiDGND, Katja Heilmann |
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DOI: | https://doi.org/10.1016/j.jim.2012.10.010 |
ISSN: | 0022-1759 |
Title of parent work (English): | Journal of immunological methods |
Publisher: | Elsevier |
Place of publishing: | Amsterdam |
Publication type: | Article |
Language: | English |
Year of first publication: | 2013 |
Publication year: | 2013 |
Release date: | 2017/03/26 |
Tag: | Hybridoma technology; Monoclonal antibody; Selection of antibody producing cells |
Volume: | 387 |
Issue: | 1-2 |
Number of pages: | 6 |
First page: | 167 |
Last Page: | 172 |
Funding institution: | Federal Ministry of Education and Research [703] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer review: | Referiert |