Rainer Bolduan, Erwin Zehe

• The objective is to compare the time scale of microbial degradation of the herbicide Isoproturon at the end of earthworm burrows with the time scale of microbial degradation in the surrounding soil matrix. To this end, we developed a method which allows the observation of microbial degradation on Isoproturon in macropores under field conditions. Study area was the well-investigated Weiherbach catchment (Kraichgau, SW Germany). The topsoil of a 12 m(2) large plot parcel was removed, the parcel was covered with a tent and instrumented with TDR and temperature sensors at two depths. After preliminary investigations to optimize application and sampling techniques, the bottom of 55 earthworm burrows, located at a depth of 80-100cm, was inoculated with Isoproturon. Within an interval of 8 d, soil material from the bottom of 5-6 earthworm burrows was taken into the laboratory and analyzed for the Isoproturon concentration for investigation of the degradation kinetics. Furthermore, the degradation of Isoproturon in the soil matrix, thatThe objective is to compare the time scale of microbial degradation of the herbicide Isoproturon at the end of earthworm burrows with the time scale of microbial degradation in the surrounding soil matrix. To this end, we developed a method which allows the observation of microbial degradation on Isoproturon in macropores under field conditions. Study area was the well-investigated Weiherbach catchment (Kraichgau, SW Germany). The topsoil of a 12 m(2) large plot parcel was removed, the parcel was covered with a tent and instrumented with TDR and temperature sensors at two depths. After preliminary investigations to optimize application and sampling techniques, the bottom of 55 earthworm burrows, located at a depth of 80-100cm, was inoculated with Isoproturon. Within an interval of 8 d, soil material from the bottom of 5-6 earthworm burrows was taken into the laboratory and analyzed for the Isoproturon concentration for investigation of the degradation kinetics. Furthermore, the degradation of Isoproturon in the soil matrix, that surrounded the macropores at the field plot, was observed in the laboratory. Microbial degradation of Isoproturon at the bottom of the earthworm burrows was with a DT-50-value of 15.6 d almost as fast as in the topsoil. In the soil matrix that closely surrounded the center of the earthworm burrows, no measurable degradation was observed within 30 d. The clearly slower degradation in the soil matrix may be likely explained by a lower microbial activity that was observed in the surrounding soil matrix. The results give evidence that deterministic modeling of the fate of pesticides once transported into heterogeneous subsoils by preferential flow requires an accuracy of a few centimeters in terms of predicting spatial locations: time scales of microbial degradation in the subsoil drop almost one order of magnitude, in case the herbicides dislocates from the bottom of an earthworm burrow a few centimeter into the surrounding soil matrix. If at all, predictions of such an accuracy can only be achieved at locations at sites where the soil hydraulic properties and the macropore system are known at a very high spatial resolution…