Investigation of the post mortem zinc protoporphyrin IX fluorescence with respect to its protein-bound and unbound occurrence in aqueous meat extracts
- Zinc protoporphyrin IX (ZnPP) is known to accumulate in most meat products during storage. However, the pathway of its formation is not yet completely clarified. To gain more insights into the specificity of ZnPP occurrence, a SEC-HPLC-UV-fluorescence setup was established to screen the proteins in aqueous meat extracts for their ZnPP fluorescence during incubation. In accordance with previous studies it was identified by SDS-PAGE and MALDI-TOF-MS that ZnPP formation takes place in myoglobin. In this study, valuable new insights into the ZnPP forming pathway were gained, as our results indicated that a significant part of ZnPP - after being formed within the protein - is transitioned into free ZnPP during incubation. Additionally, the obtained results implied that ZnPP may also occur in proteins of higher molecular weight (> 100 kDa).
| Author details: | Amin Ghadiri KhozroughiGND, Tess Waldbach Braga, Janine Wagner, Harshadrai Manilal RawelORCiDGND |
|---|---|
| DOI: | https://doi.org/10.1016/j.foodchem.2019.01.080 |
| ISSN: | 0308-8146 |
| ISSN: | 1873-7072 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30722899 |
| Title of parent work (English): | Food chemistry |
| Publisher: | Elsevier |
| Place of publishing: | Oxford |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2019 |
| Publication year: | 2019 |
| Release date: | 2021/01/21 |
| Tag: | Fluorescence screening; MALDI-TOF-MS; Meat; Post mortem chemistry; SDS-PAGE; SEC-HPLC |
| Volume: | 283 |
| Number of pages: | 6 |
| First page: | 462 |
| Last Page: | 467 |
| Funding institution: | Avicenna foundation from the Federal Ministry of Education and Research of Germany |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Ernährungswissenschaft |
| DDC classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Peer review: | Referiert |
| Publishing method: | Open Access / Bronze Open-Access |
