Structural and functional analysis of an l-serine O-phosphate decarboxylase involved in norcobamide biosynthesis
- Structural diversity of natural cobamides (Cbas, B12 vitamers) is limited to the nucleotide loop. The loop is connected to the cobalt‐containing corrin ring via an (R)‐1‐aminopropan‐2‐ol O‐2‐phosphate (AP‐P) linker moiety. AP‐P is produced by the l‐threonine O‐3‐phosphate (l‐Thr‐P) decarboxylase CobD. Here, the CobD homolog SMUL_1544 of the organohalide‐respiring epsilonproteobacterium Sulfurospirillum multivorans was characterized as a decarboxylase that produces ethanolamine O‐phosphate (EA‐P) from l‐serine O‐phosphate (l‐Ser‐P). EA‐P is assumed to serve as precursor of the linker moiety of norcobamides that function as cofactors in the respiratory reductive dehalogenase. SMUL_1544 (SmCobD) is a pyridoxal‐5′‐phosphate (PLP)‐containing enzyme. The structural analysis of the SmCobD apoprotein combined with the characterization of truncated mutant proteins uncovered a role of the SmCobD N‐terminus in efficient l‐Ser‐P conversion.
Author details: | Sebastian Keller, Karl M. Wetterhorn, Alison Vecellio, Mark Seeger, Ivan Rayment, Torsten SchubertORCiD |
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DOI: | https://doi.org/10.1002/1873-3468.13543 |
ISSN: | 0014-5793 |
ISSN: | 1873-3468 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31325159 |
Title of parent work (English): | FEBS letters : the journal for rapid publication of short reports in molecular biosciences |
Publisher: | Wiley |
Place of publishing: | Hoboken |
Publication type: | Article |
Language: | English |
Date of first publication: | 2019/07/19 |
Publication year: | 2019 |
Release date: | 2020/12/14 |
Tag: | cobamides; ethanolamine phosphate; norcobamide biosynthesis; serine phosphate decarboxylase |
Volume: | 593 |
Issue: | 21 |
Number of pages: | 14 |
First page: | 3040 |
Last Page: | 3053 |
Funding institution: | German Research Foundation (DFG)German Research Foundation (DFG) [SCHU 2605/1-1]; Strategy and Innovation Grant from Free State of Thuringia [41-5507-2016]; Leibniz Research Cluster InfectoOptics [SAS-2015-HKI]; U.S. Department of Energy, Office of Energy ResearchUnited States Department of Energy (DOE); [R35-GM130399] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer review: | Referiert |
Publishing method: | Open Access / Hybrid Open-Access |