Stimuli-responsive membrane activity of cyclic-peptide-polymer conjugates
- Cyclic peptide nanotubes (CPNT) consisting of an even number of amino acids with an alternating chirality are highly interesting materials in a biomedical context due to their ability to insert themselves into cellular membranes. However, unwanted unspecific interactions between CPNT and non-targeted cell membranes are a major drawback. To solve this issue we have synthetized a series of CPNT-polymer conjugates with a cleavable covalent connection between macromolecule and peptide. As a result, the polymers form a stabilizing and shielding shell around the nanotube that can be cleaved on demand to generate membrane active CPNT from non-active conjugates. This approach enables us to control the stacking and lateral aggregation of these materials, thus leading to stimuli responsive membrane activity. Moreover, upon activation, the systems can be adjusted to form nanotubes with an increased length instead of aggregates. We were able to study the dynamics of these systems in detail and prove the concept of stimuli responsive membraneCyclic peptide nanotubes (CPNT) consisting of an even number of amino acids with an alternating chirality are highly interesting materials in a biomedical context due to their ability to insert themselves into cellular membranes. However, unwanted unspecific interactions between CPNT and non-targeted cell membranes are a major drawback. To solve this issue we have synthetized a series of CPNT-polymer conjugates with a cleavable covalent connection between macromolecule and peptide. As a result, the polymers form a stabilizing and shielding shell around the nanotube that can be cleaved on demand to generate membrane active CPNT from non-active conjugates. This approach enables us to control the stacking and lateral aggregation of these materials, thus leading to stimuli responsive membrane activity. Moreover, upon activation, the systems can be adjusted to form nanotubes with an increased length instead of aggregates. We were able to study the dynamics of these systems in detail and prove the concept of stimuli responsive membrane interaction using CPNT-polymer conjugates to permeabilize liposomes as well as mammalian cell membranes.…
Author details: | Matthias HartliebORCiDGND, Sylvain Catrouillet, Agnes Kuroki, Carlos Sanchez-CanoORCiD, Raoul PeltierORCiD, Sebastien PerrierORCiD |
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DOI: | https://doi.org/10.1039/c9sc00756c |
ISSN: | 2041-6520 |
ISSN: | 2041-6539 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31293730 |
Title of parent work (English): | Chemical science |
Publisher: | Royal Society of Chemistry |
Place of publishing: | Cambridge |
Publication type: | Article |
Language: | English |
Date of first publication: | 2019/04/18 |
Publication year: | 2019 |
Release date: | 2021/01/25 |
Volume: | 10 |
Issue: | 21 |
Number of pages: | 8 |
First page: | 5476 |
Last Page: | 5483 |
Funding institution: | Royal Society Wolfson Merit AwardRoyal Society of London [WM130055]; European Research CouncilEuropean Research Council (ERC) [TUSUPO 647106]; CRUK/EPSRCEngineering & Physical Sciences Research Council (EPSRC)Cancer Research UK [C53561/A19933]; German Research Foundation (DFG)German Research Foundation (DFG) [GZ: HA 7725/1-1]; Monash-Warwick Alliance; STFCScience & Technology Facilities Council (STFC) [9-13-668] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Peer review: | Referiert |
Publishing method: | Open Access / Gold Open-Access |
DOAJ gelistet | |
License (English): | Creative Commons - Namensnennung 3.0 Unported |