Toxin-antigen conjugates as selection tools for antibody producing cells
- The generation of antibodies with designated specificity requires cost-intensive and time-consuming screening procedures. Here we present a new method by which hybridoma cells can be selected based on the specificity of the produced antibody by the use of antigen-toxin-conjugates thus eliminating the need of a screening procedure. Initial experiments were done with methotrexate as low molecular weight toxin and fluorescein as model antigen. Methotrexate and a methotrexate-fluorescein conjugate were characterized regarding their toxicity. Afterwards the effect of the fluorescein-specific antibody B13-DE1 on the toxicity of the methotrexate-fluorescein conjugate was determined. Finally, first results showed that hybridoma cells that produce fluorescein specific antibodies are able to grow in the presence of fluorescein-toxin-conjugates.
Verfasserangaben: | Katrin MesserschmidtORCiDGND, Katja Heilmann |
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DOI: | https://doi.org/10.1016/j.jim.2012.10.010 |
ISSN: | 0022-1759 |
Titel des übergeordneten Werks (Englisch): | Journal of immunological methods |
Verlag: | Elsevier |
Verlagsort: | Amsterdam |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Erstveröffentlichung: | 2013 |
Erscheinungsjahr: | 2013 |
Datum der Freischaltung: | 26.03.2017 |
Freies Schlagwort / Tag: | Hybridoma technology; Monoclonal antibody; Selection of antibody producing cells |
Band: | 387 |
Ausgabe: | 1-2 |
Seitenanzahl: | 6 |
Erste Seite: | 167 |
Letzte Seite: | 172 |
Fördernde Institution: | Federal Ministry of Education and Research [703] |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer Review: | Referiert |