Biomineralization of engineered spider silk protein-based composite materials for bone tissue engineering
- Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.
Author details: | John G. Hardy, Jose Guillermo Torres-Rendon, Aldo Leal-Egaña, Andreas Walther, Helmut SchlaadORCiDGND, Helmut CölfenGND, Thomas R. ScheibelORCiDGND |
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URN: | urn:nbn:de:kobv:517-opus4-400519 |
Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (359) |
Publication type: | Postprint |
Language: | English |
Date of first publication: | 2017/09/22 |
Publication year: | 2017 |
Publishing institution: | Universität Potsdam |
Release date: | 2017/09/22 |
Tag: | biodegradable polymers; biomaterials; biomineralization; bone tissue engineering; recombinant protein; spider silk |
Number of pages: | 13 |
Source: | Materials 9 (2016) Nr. 7. - DOI: 10.3390/ma9070560 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Chemie |
DDC classification: | 6 Technik, Medizin, angewandte Wissenschaften / 60 Technik / 600 Technik, Technologie |
Peer review: | Referiert |
Publishing method: | Open Access |
Grantor: | Multidisciplinary Digital Publishing Institute (MDPI) |
License (German): | CC-BY - Namensnennung 4.0 International |